Restriction map for
pFN21AE2261
(Circular) (Six-base) MAPSORT of: pFN21AE2261.seq Check: 1886 from: 1 to: 6883
pFN21AE2261 6883 bp
With 182 enzymes: SgfI *
March 28, 2012 12:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3395 3395
Size: 6883
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4693 278
Size: 53 83 186 936 3157 2468
Fragments arranged by size:
3157 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2196 4226 1937
Size: 259 2030 4594
Fragments arranged by size:
4594 2030 259
Acc65I G'GTAC_C
Cuts at: 4204 4204
Size: 6883
AclI AA'CG_TT
Cuts at: 1563 5010 5383 1563
Size: 3447 373 3063
Fragments arranged by size:
3447 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2123 2823 2862 3288 3756 4946 6330 6598
Size: 491 700 39 426 468 1190 1384 268
Cuts at: 6598 1632
Size: 1917
Fragments arranged by size:
1917 1384 1190 700 491 468 426 268
39
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6686
AflIII A'CryG_T
Cuts at: 1203 2311 5782 6759 1203
Size: 1108 3471 977 1327
Fragments arranged by size:
3471 1327 1108 977
AgeI A'CCGG_T
Cuts at: 5714 5714
Size: 6883
AhdI GACnn_n'nnGTC
Cuts at: 5612 5612
Size: 6883
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6883
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2441 1184
Size: 1257 5626
AlwNI CAG_nnn'CTG
Cuts at: 4626 6198 4626
Size: 1572 5311
ApaI G_GGCC'C
Cuts at: 3933 3933
Size: 6883
ApaLI G'TGCA_C
Cuts at: 2482 4940 6096 2482
Size: 2458 1156 3269
Fragments arranged by size:
3269 2458 1156
ApoI r'AATT_y
Cuts at: 1493 2245 3064 3805 4049 4192 4418 4570 6679
Size: 752 819 741 244 143 226 152 2109
Cuts at: 6679 1493
Size: 1697
Fragments arranged by size:
2109 1697 819 752 741 244 226 152
143
AseI AT'TA_AT
Cuts at: 160 1032 5437 160
Size: 872 4405 1606
Fragments arranged by size:
4405 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6883
AvaI C'yCGr_G
Cuts at: 1870 1943 2049 4102 4208 1870
Size: 73 106 2053 106 4545
Fragments arranged by size:
4545 2053 106 106 73
BamHI G'GATC_C
Cuts at: 4213 4580 4213
Size: 367 6516
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4204 5659 618
Size: 325 217 627 2417 1455 1842
Fragments arranged by size:
2417 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2876 3933 4202 1391
Size: 1485 1057 269 4072
Fragments arranged by size:
4072 1485 1057 269
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3417 928
Size: 971 1518 4394
Fragments arranged by size:
4394 1518 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5074 5108 1903
Size: 34 3137 34 3678
Fragments arranged by size:
3678 3137 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4775 5985 4775
Size: 1210 5673
BclI T'GATC_A
Cuts at: 1548 2455 3085 3760 1548
Size: 907 630 675 4671
Fragments arranged by size:
4671 907 675 630
BfrBI ATG'CAT
Cuts at: 2203 3116 3260 2203
Size: 913 144 5826
Fragments arranged by size:
5826 913 144
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5494 136
Size: 107 122 71 631 605 3822 1525
Fragments arranged by size:
3822 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2398 4106 6878 2398
Size: 1708 2772 2403
Fragments arranged by size:
2772 2403 1708
BlpI GC'TnA_GC
Cuts at: 4300 4300
Size: 6883
Bme1580I G_kGCm'C
Cuts at: 1790 2486 3933 4015 4944 6100 1790
Size: 696 1447 82 929 1156 2573
Fragments arranged by size:
2573 1447 1156 929 696 82
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6883
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2636 3392 3624
Size: 416 53 83 399 24 1219 756 232
Cuts at: 3624 3945 4614 5572 442
Size: 321 669 958 1753
Fragments arranged by size:
1753 1219 958 756 669 416 399 321
232 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6883
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2241 3245 3765 5543 1239
Size: 1002 1004 520 1778 2579
Fragments arranged by size:
2579 1778 1004 1002 520
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5008 5873 6171 5008
Size: 865 298 5720
Fragments arranged by size:
5720 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5546 882
Size: 617 4047 2219
Fragments arranged by size:
4047 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4674 493
Size: 711 3470 2702
Fragments arranged by size:
3470 2702 711
BsaBI GATnn'nnATC
Cuts at: 6877 6877
Size: 6883
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2682 4690 5072 5707
Size: 53 83 186 936 1149 2008 382 635
Cuts at: 5707 275
Size: 1451
Fragments arranged by size:
2008 1451 1149 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5316 5714 5988 6135 6546 5316
Size: 398 274 147 411 5653
Fragments arranged by size:
5653 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3738 3768 1178
Size: 30 165 30 2335 30 4293
Fragments arranged by size:
4293 2335 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2303 3452 4090 4146 1186
Size: 1117 1149 638 56 3923
Fragments arranged by size:
3923 1149 1117 638 56
BseYI C'CCAG_C
Cuts at: 3887 6086 6582 3887
Size: 2199 496 4188
Fragments arranged by size:
4188 2199 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2949 1163
Size: 573 1213 5097
Fragments arranged by size:
5097 1213 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4333 5094 6122 1540
Size: 275 186 2332 761 1028 2301
Fragments arranged by size:
2332 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2486 2876 3522 4944 5029 6100 2486
Size: 390 646 1422 85 1071 3269
Fragments arranged by size:
3269 1422 1071 646 390 85
BsmI GAATG_Cn'
Cuts at: 2604 4394 4487 2604
Size: 1790 93 5000
Fragments arranged by size:
5000 1790 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2170 2170
Size: 6883
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2486 2876 3522 3933 4015 4202 4944
Size: 399 696 390 646 411 82 187 742
Cuts at: 4944 5029 6100 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 696 646 411 399 390
187 85 82
BspEI T'CCGG_A
Cuts at: 6546 6546
Size: 6883
BspHI T'CATG_A
Cuts at: 3289 4772 6502 3289
Size: 1483 1730 3670
Fragments arranged by size:
3670 1730 1483
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2885 3395 4238 844
Size: 2041 510 843 3489
Fragments arranged by size:
3489 2041 843 510
BsrBI CCG'CTC
Cuts at: 1394 1823 3349 4770 1394
Size: 429 1526 1421 3507
Fragments arranged by size:
3507 1526 1421 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2889 5378 5552 65
Size: 1183 1641 2489 174 1396
Fragments arranged by size:
2489 1641 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5527 5714 1700
Size: 110 3717 187 2869
Fragments arranged by size:
3717 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6883
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6883
BssSI C'ACGA_G
Cuts at: 3900 4943 5955 3900
Size: 1043 1012 4828
Fragments arranged by size:
4828 1043 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3266 794
Size: 2472 4411
BstBI TT'CG_AA
Cuts at: 3914 5687 3914
Size: 1773 5110
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6883
BstXI CCAn_nnnn'nTGG
Cuts at: 2305 2305
Size: 6883
BstYI r'GATC_y
Cuts at: 1972 2398 2637 4106 4213 4580 4965 4982 6434
Size: 426 239 1469 107 367 385 17 1452
Cuts at: 6434 6520 6532 6878 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1469 1452 426 385 367 346 239
107 86 17 12
BstZ17I GTA'TAC
Cuts at: 2197 2197
Size: 6883
Bsu36I CC'TnA_GG
Cuts at: 2346 4179 2346
Size: 1833 5050
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5392
Fragments arranged by size:
5392 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6144
Fragments arranged by size:
6144 683 56
BtsI GCAGTG_nn'
Cuts at: 2184 2937 4399 5193 5213 2184
Size: 753 1462 794 20 3854
Fragments arranged by size:
3854 1462 794 753 20
ClaI AT'CG_AT
Cuts at: 1554 2206 1554
Size: 652 6231
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6848
DraI TTT'AAA
Cuts at: 4187 4187
Size: 6883
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5890 809
Size: 507 4574 1802
Fragments arranged by size:
4574 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2127 4130 4330
Size: 54 1425 50 275 18 297 2003 200
Cuts at: 4330 5219 6848 8
Size: 889 1629 43
Fragments arranged by size:
2003 1629 1425 889 297 275 200 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4330 1537
Size: 275 2518 4090
Fragments arranged by size:
4090 2518 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2881 2949 3209 3709 3732 3802 4813 1353
Size: 1528 68 260 500 23 70 1011 3423
Fragments arranged by size:
3423 1528 1011 500 260 70 68 23
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5470 5844 5990 387
Size: 1064 138 3881 374 146 1280
Fragments arranged by size:
3881 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2874 2874
Size: 6883
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2123 2241 2823 2862 3245 3288 3756
Size: 393 491 118 582 39 383 43 468
Cuts at: 3756 3765 4946 5543 6330 6598 1239
Size: 9 1181 597 787 268 1524
Fragments arranged by size:
1524 1181 787 597 582 491 468 393
383 268 118 43 39 9
EcoNI CCTnn'n_nnAGG
Cuts at: 4178 4178
Size: 6883
EcoO109I rG'GnC_Cy
Cuts at: 3728 3929 3728
Size: 201 6682
EcoRI G'AATT_C
Cuts at: 4192 4570 4192
Size: 378 6505
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2394 2394
Size: 6883
FspI TGC'GCA
Cuts at: 3452 5389 3452
Size: 1937 4946
FspAI rTGC'GCAy
Cuts at: 3452 3452
Size: 6883
HaeII r_GCGC'y
Cuts at: 1678 6030 1678
Size: 4352 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5524 5556 5598 5630 1373
Size: 32 135 32 3952 32 42 32 2626
Fragments arranged by size:
3952 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2680 2913 4227 4473 669
Size: 988 281 742 233 1314 246 3079
Fragments arranged by size:
3079 1314 988 742 281 246 233
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6883
HpaI GTT'AAC
Cuts at: 4473 4473
Size: 6883
KpnI G_GTAC'C
Cuts at: 4208 4208
Size: 6883
MfeI C'AATT_G
Cuts at: 2171 2451 2629 4482 2171
Size: 280 178 1853 4572
Fragments arranged by size:
4572 1853 280 178
MluI A'CGCG_T
Cuts at: 6759 6759
Size: 6883
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5997 6181 1305
Size: 4692 184 2007
Fragments arranged by size:
4692 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5061
Fragments arranged by size:
5061 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2469 4841 5200 5359 518
Size: 648 1303 2372 359 159 2042
Fragments arranged by size:
2372 2042 1303 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2059 3473 3891 4299 4976 6124 6369 1606
Size: 453 1414 418 408 677 1148 245 2120
Fragments arranged by size:
2120 1414 1148 677 453 418 408 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5392
Fragments arranged by size:
5392 939 552
NdeI CA'TA_TG
Cuts at: 387 3262 387
Size: 2875 4008
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6883
NotI GC'GGCC_GC
Cuts at: 4330 4330
Size: 6883
NsiI A_TGCA'T
Cuts at: 2205 3118 3262 2205
Size: 913 144 5826
Fragments arranged by size:
5826 913 144
NspI r_CATG'y
Cuts at: 1132 1229 2203 2315 2787 2911 3194 4241 5786
Size: 97 974 112 472 124 283 1047 1545
Cuts at: 5786 1132
Size: 2229
Fragments arranged by size:
2229 1545 1047 974 472 283 124 112
97
PasI CC'CwG_GG
Cuts at: 1348 4015 1348
Size: 2667 4216
PciI A'CATG_T
Cuts at: 2311 5782 2311
Size: 3471 3412
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3104 1108
Size: 1996 4887
PmeI GTTT'AAAC
Cuts at: 4187 4187
Size: 6883
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4952 4984 6465 6497 4952
Size: 32 1481 32 5338
Fragments arranged by size:
5338 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3728 3728
Size: 6883
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6883
PsiI TTA'TAA
Cuts at: 4453 4453
Size: 6883
PspOMI G'GGCC_C
Cuts at: 3929 3929
Size: 6883
PstI C_TGCA'G
Cuts at: 830 2574 3472 4235 830
Size: 1744 898 763 3478
Fragments arranged by size:
3478 1744 898 763
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6883
PvuII CAG'CTG
Cuts at: 2059 3473 3891 2059
Size: 1414 418 5051
Fragments arranged by size:
5051 1414 418
SacI G_AGCT'C
Cuts at: 2876 2876
Size: 6883
SalI G'TCGA_C
Cuts at: 1936 4225 1936
Size: 2289 4594
SanDI GG'GwC_CC
Cuts at: 3728 3728
Size: 6883
SapI GCTCTTCn'nnn_
Cuts at: 2881 2881
Size: 6883
SbfI CC_TGCA'GG
Cuts at: 4235 4235
Size: 6883
ScaI AGT'ACT
Cuts at: 3787 5131 6843 3787
Size: 1344 1712 3827
Fragments arranged by size:
3827 1712 1344
SfcI C'TryA_G
Cuts at: 826 1045 2190 2570 2729 3468 3489 3679 4231
Size: 219 1145 380 159 739 21 190 552
Cuts at: 4231 5366 6047 6238 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1145 1135 739 681 552 380 219
191 190 159 21
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6883
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6883
SmaI CCC'GGG
Cuts at: 1872 4210 1872
Size: 2338 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2049 4102 4987 5888 6150 820
Size: 197 926 106 2053 885 901 262 1553
Fragments arranged by size:
2053 1553 926 901 885 262 197 106
SnaBI TAC'GTA
Cuts at: 493 4674 493
Size: 4181 2702
SpeI A'CTAG_T
Cuts at: 152 3056 152
Size: 2904 3979
SphI G_CATG'C
Cuts at: 1132 2787 3194 4241 1132
Size: 1655 407 1047 3774
Fragments arranged by size:
3774 1655 1047 407
SspI AAT'ATT
Cuts at: 5 52 4807 5
Size: 47 4755 2081
Fragments arranged by size:
4755 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6883
StyI C'CwwG_G
Cuts at: 513 1065 2004 2694 4322 513
Size: 552 939 690 1628 3074
Fragments arranged by size:
3074 1628 939 690 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2475 2832 3558 4074 4910 4927 5080
Size: 1255 895 357 726 516 836 17 153
Cuts at: 5080 5265 5710 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 895 836 726 516 445 357
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2574 3785 5129
Size: 274 80 33 51 1443 597 1211 1344
Cuts at: 5129 6841 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1344 1211 597 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2235 2267 3578 3610 3738 3770 6843 6875 2235
Size: 32 1311 32 128 32 3073 32 2243
Fragments arranged by size:
3073 2243 1311 128 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6883
XbaI T'CTAG_A
Cuts at: 4219 4219
Size: 6883
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2692 3605 2692
Size: 913 5970
XhoI C'TCGA_G
Cuts at: 1943 2049 4102 1943
Size: 106 2053 4724
Fragments arranged by size:
4724 2053 106
XmaI C'CCGG_G
Cuts at: 1870 4208 1870
Size: 2338 4545
XmnI GAAnn'nnTTC
Cuts at: 2355 4053 5012 2355
Size: 1698 959 4226
Fragments arranged by size:
4226 1698 959
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4691 276
Size: 53 83 186 936 3157 2468
Fragments arranged by size:
3157 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SanDI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI
Bpu10I BsiWI DraIII EcoRV FseI KasI NaeI NarI
NgoMIV NruI PacI PfoI PmlI PspXI PsrI RsrII
SacII SexAI SfiI SfoI SrfI SwaI