Restriction map for
pFN21AE2256
(Circular) (Six-base) MAPSORT of: pFN21AE2256.seq Check: 2136 from: 1 to: 5962
pFN21AE2256 5962 bp
With 182 enzymes: SgfI *
July 20, 2018 18:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2352 3772 278
Size: 53 83 186 936 816 1420 2468
Fragments arranged by size:
2468 1420 936 816 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2280 2586 3305 1937
Size: 343 306 719 4594
Fragments arranged by size:
4594 719 343 306
Acc65I G'GTAC_C
Cuts at: 3283 3283
Size: 5962
AclI AA'CG_TT
Cuts at: 1563 4089 4462 1563
Size: 2526 373 3063
Fragments arranged by size:
3063 2526 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4025 5409 5677 1632
Size: 2393 1384 268 1917
Fragments arranged by size:
2393 1917 1384 268
AfeI AGC'GCT
Cuts at: 2666 2666
Size: 5962
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5765
AflIII A'CryG_T
Cuts at: 1203 4861 5838 1203
Size: 3658 977 1327
Fragments arranged by size:
3658 1327 977
AgeI A'CCGG_T
Cuts at: 4793 4793
Size: 5962
AhdI GACnn_n'nnGTC
Cuts at: 4691 4691
Size: 5962
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5962
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3263 1184
Size: 2079 3883
AlwNI CAG_nnn'CTG
Cuts at: 2464 3115 3705 5277 2464
Size: 651 590 1572 3149
Fragments arranged by size:
3149 1572 651 590
ApaLI G'TGCA_C
Cuts at: 2359 3098 4019 5175 2359
Size: 739 921 1156 3146
Fragments arranged by size:
3146 1156 921 739
ApoI r'AATT_y
Cuts at: 1493 3271 3497 3649 5758 1493
Size: 1778 226 152 2109 1697
Fragments arranged by size:
2109 1778 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4516 160
Size: 872 3484 1606
Fragments arranged by size:
3484 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5962
AvaI C'yCGr_G
Cuts at: 1870 1943 2814 3287 1870
Size: 73 871 473 4545
Fragments arranged by size:
4545 871 473 73
BamHI G'GATC_C
Cuts at: 3292 3659 3292
Size: 367 5595
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2474 2506 3283 4738 618
Size: 325 217 627 687 32 777 1455 1842
Fragments arranged by size:
1842 1455 777 687 627 325 217 32
BanII G_rGCy'C
Cuts at: 1391 2318 3281 1391
Size: 927 963 4072
Fragments arranged by size:
4072 963 927
BbeI G_GCGC'C
Cuts at: 2478 2510 2478
Size: 32 5930
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3202 3226 928
Size: 971 1303 24 3664
Fragments arranged by size:
3664 1303 971 24
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4153 4187 1903
Size: 34 2216 34 3678
Fragments arranged by size:
3678 2216 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3854 5064 3854
Size: 1210 4752
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5962
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2066 2339 3142
Size: 107 122 71 631 605 394 273 803
Cuts at: 3142 4573 136
Size: 1431 1525
Fragments arranged by size:
1525 1431 803 631 605 394 273 122
107 71
BglII A'GATC_T
Cuts at: 5957 5957
Size: 5962
BlpI GC'TnA_GC
Cuts at: 2312 3379 2312
Size: 1067 4895
Bme1580I G_kGCm'C
Cuts at: 1790 2363 3102 3138 4023 5179 1790
Size: 573 739 36 885 1156 2573
Fragments arranged by size:
2573 1156 885 739 573 36
BmgBI CAC'GTC
Cuts at: 1318 2533 2681 1318
Size: 1215 148 4599
Fragments arranged by size:
4599 1215 148
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2650 3693 4651
Size: 416 53 83 399 24 1233 1043 958
Cuts at: 4651 442
Size: 1753
Fragments arranged by size:
1753 1233 1043 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5962
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2585 4622 1239
Size: 1346 2037 2579
Fragments arranged by size:
2579 2037 1346
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4087 4952 5250 4087
Size: 865 298 4799
Fragments arranged by size:
4799 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4625 882
Size: 617 3126 2219
Fragments arranged by size:
3126 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2481 2581 3753 493
Size: 711 1277 100 1172 2702
Fragments arranged by size:
2702 1277 1172 711 100
BsaBI GATnn'nnATC
Cuts at: 5956 5956
Size: 5962
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2204 2331 2349 2475
Size: 53 83 186 936 671 127 18 126
Cuts at: 2475 2507 2946 3083 3769 4151 4786 275
Size: 32 439 137 686 382 635 1451
Fragments arranged by size:
1451 936 686 671 635 439 382 186
137 127 126 83 53 32 18
BsaWI w'CCGG_w
Cuts at: 4395 4793 5067 5214 5625 4395
Size: 398 274 147 411 4732
Fragments arranged by size:
4732 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2989 3019 1178
Size: 30 165 30 1586 30 4121
Fragments arranged by size:
4121 1586 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2237 2240 1186
Size: 1051 3 4908
Fragments arranged by size:
4908 1051 3
BseYI C'CCAG_C
Cuts at: 2045 2096 2667 2717 2852 3166 5165 5661 2045
Size: 51 571 50 135 314 1999 496 2346
Fragments arranged by size:
2346 1999 571 496 314 135 51 50
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2678 1163
Size: 573 942 4447
Fragments arranged by size:
4447 942 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2061 2238 2542 2701 3022 3412
Size: 275 186 60 177 304 159 321 390
Cuts at: 3412 4173 5201 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1028 761 390 321 304 275 186
177 159 60
BsiHKAI G_wGCw'C
Cuts at: 2151 2363 2603 2938 3102 4023 4108 5179 2151
Size: 212 240 335 164 921 85 1071 2934
Fragments arranged by size:
2934 1071 921 335 240 212 164 85
BsmI GAATG_Cn'
Cuts at: 3473 3566 3473
Size: 93 5869
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2151 2318 2363 2603 2938 3102 3138
Size: 399 361 167 45 240 335 164 36
Cuts at: 3138 3281 4023 4108 5179 1391
Size: 143 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 361 335 240 167
164 143 85 45 36
BspEI T'CCGG_A
Cuts at: 5625 5625
Size: 5962
BspHI T'CATG_A
Cuts at: 3851 5581 3851
Size: 1730 4232
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3317 844
Size: 2473 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2272 2623 3849 1394
Size: 429 449 351 1226 3507
Fragments arranged by size:
3507 1226 449 429 351
BsrDI GCAATG_nn'
Cuts at: 65 1248 4457 4631 65
Size: 1183 3209 174 1396
Fragments arranged by size:
3209 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2056 2436 2454 3017 3049 4606 4793
Size: 110 246 380 18 563 32 1557 187
Cuts at: 4793 1700
Size: 2869
Fragments arranged by size:
2869 1557 563 380 246 187 110 32
18
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5962
BssHII G'CGCG_C
Cuts at: 1925 2191 2703 1925
Size: 266 512 5184
Fragments arranged by size:
5184 512 266
BssSI C'ACGA_G
Cuts at: 2165 4022 5034 2165
Size: 1857 1012 3093
Fragments arranged by size:
3093 1857 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2464 794
Size: 1670 4292
BstBI TT'CG_AA
Cuts at: 4766 4766
Size: 5962
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5962
BstXI CCAn_nnnn'nTGG
Cuts at: 2395 2395
Size: 5962
BstYI r'GATC_y
Cuts at: 1972 3292 3659 4044 4061 5513 5599 5611 5957
Size: 1320 367 385 17 1452 86 12 346
Cuts at: 5957 1972
Size: 1977
Fragments arranged by size:
1977 1452 1320 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2022 2061 2343 2412 2493 2653
Size: 552 939 18 39 282 69 81 160
Cuts at: 2653 3140 513
Size: 487 3335
Fragments arranged by size:
3335 939 552 487 282 160 81 69
39 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5223
Fragments arranged by size:
5223 683 56
BtsI GCAGTG_nn'
Cuts at: 3478 4272 4292 3478
Size: 794 20 5148
Fragments arranged by size:
5148 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5962
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5927
DraI TTT'AAA
Cuts at: 3266 3266
Size: 5962
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2582 4969 809
Size: 507 1266 2387 1802
Fragments arranged by size:
2387 1802 1266 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2058 2181 2235
Size: 54 1425 50 275 18 228 123 54
Cuts at: 2235 2340 2409 2415 2539 2698 3019 3409 4298
Size: 105 69 6 124 159 321 390 889
Cuts at: 4298 5927 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 390 321 275 228 159
124 123 105 69 54 54 50 43
18 6
EagI C'GGCC_G
Cuts at: 1537 1812 2058 2235 2539 2698 3019 3409 1537
Size: 275 246 177 304 159 321 390 4090
Fragments arranged by size:
4090 390 321 304 275 246 177 159
EarI CTCTTCn'nnn_
Cuts at: 1353 2279 3892 1353
Size: 926 1613 3423
Fragments arranged by size:
3423 1613 926
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4549 4923 5069 387
Size: 1064 138 2960 374 146 1280
Fragments arranged by size:
2960 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2585 4025 4622 5409 5677 1239
Size: 393 953 1440 597 787 268 1524
Fragments arranged by size:
1524 1440 953 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2765 2861 2765
Size: 96 5866
EcoO109I rG'GnC_Cy
Cuts at: 2770 2819 2866 3043 3063 3244 2770
Size: 49 47 177 20 181 5488
Fragments arranged by size:
5488 181 177 49 47 20
EcoRI G'AATT_C
Cuts at: 3271 3649 3271
Size: 378 5584
FseI GG_CCGG'CC
Cuts at: 2060 2060
Size: 5962
FspI TGC'GCA
Cuts at: 4468 4468
Size: 5962
HaeII r_GCGC'y
Cuts at: 1678 2117 2478 2510 2668 3116 3156 3186 5109
Size: 439 361 32 158 448 40 30 1923
Cuts at: 5109 1678
Size: 2531
Fragments arranged by size:
2531 1923 448 439 361 158 40 32
30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4603 4635 4677 4709 1373
Size: 32 135 32 3031 32 42 32 2626
Fragments arranged by size:
3031 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3178 3306 3552 669
Size: 988 281 1240 128 246 3079
Fragments arranged by size:
3079 1240 988 281 246 128
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5962
HpaI GTT'AAC
Cuts at: 3552 3552
Size: 5962
KasI G'GCGC_C
Cuts at: 2474 2506 2474
Size: 32 5930
KpnI G_GTAC'C
Cuts at: 3287 3287
Size: 5962
MfeI C'AATT_G
Cuts at: 3561 3561
Size: 5962
MluI A'CGCG_T
Cuts at: 5838 5838
Size: 5962
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2952 5076 5260 1305
Size: 1647 2124 184 2007
Fragments arranged by size:
2124 2007 1647 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2183 2342 10
Size: 54 1425 343 351 159 3630
Fragments arranged by size:
3630 1425 351 343 159 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2342 2393 3920 4279 4438 518
Size: 648 1176 51 1527 359 159 2042
Fragments arranged by size:
2042 1527 1176 648 359 159 51
MspA1I CmG'CkG
Cuts at: 1606 2024 2063 2425 2461 2495 2655 2721 3142
Size: 418 39 362 36 34 160 66 421
Cuts at: 3142 3378 4055 5203 5448 1606
Size: 236 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 421 418 362 245 236
160 66 39 36 34
NaeI GCC'GGC
Cuts at: 2058 2456 2058
Size: 398 5564
NarI GG'CG_CC
Cuts at: 2475 2507 2475
Size: 32 5930
NcoI C'CATG_G
Cuts at: 513 1065 2004 2343 513
Size: 552 939 339 4132
Fragments arranged by size:
4132 939 552 339
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5962
NgoMIV G'CCGG_C
Cuts at: 2056 2454 2056
Size: 398 5564
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5962
NotI GC'GGCC_GC
Cuts at: 2235 2539 3409 2235
Size: 304 870 4788
Fragments arranged by size:
4788 870 304
NspI r_CATG'y
Cuts at: 1132 1229 3320 4865 1132
Size: 97 2091 1545 2229
Fragments arranged by size:
2229 2091 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5962
PciI A'CATG_T
Cuts at: 4861 4861
Size: 5962
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5962
PmeI GTTT'AAAC
Cuts at: 3266 3266
Size: 5962
PmlI CAC'GTG
Cuts at: 2481 2481
Size: 5962
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4031 4063 5544 5576 4031
Size: 32 1481 32 4417
Fragments arranged by size:
4417 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2770 2819 2866 3043 2770
Size: 49 47 177 5689
Fragments arranged by size:
5689 177 49 47
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5962
PsiI TTA'TAA
Cuts at: 3532 3532
Size: 5962
PstI C_TGCA'G
Cuts at: 830 2202 2664 3314 830
Size: 1372 462 650 3478
Fragments arranged by size:
3478 1372 650 462
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5962
PvuII CAG'CTG
Cuts at: 2461 2461
Size: 5962
SacII CC_GC'GG
Cuts at: 2025 2064 2496 2656 3143 2025
Size: 39 432 160 487 4844
Fragments arranged by size:
4844 487 432 160 39
SalI G'TCGA_C
Cuts at: 1936 3304 1936
Size: 1368 4594
SanDI GG'GwC_CC
Cuts at: 2819 2819
Size: 5962
SapI GCTCTTCn'nnn_
Cuts at: 2279 2279
Size: 5962
SbfI CC_TGCA'GG
Cuts at: 3314 3314
Size: 5962
ScaI AGT'ACT
Cuts at: 4210 5922 4210
Size: 1712 4250
SfcI C'TryA_G
Cuts at: 826 1045 2198 2660 3310 4445 5126 5317 826
Size: 219 1153 462 650 1135 681 191 1471
Fragments arranged by size:
1471 1153 1135 681 650 462 219 191
SfoI GGC'GCC
Cuts at: 2476 2508 2476
Size: 32 5930
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5962
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5962
SmaI CCC'GGG
Cuts at: 1872 2816 3289 1872
Size: 944 473 4545
Fragments arranged by size:
4545 944 473
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4066 4967 5229 820
Size: 197 926 2123 901 262 1553
Fragments arranged by size:
2123 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3753 493
Size: 3260 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5962
SphI G_CATG'C
Cuts at: 1132 3320 1132
Size: 2188 3774
SspI AAT'ATT
Cuts at: 5 52 3886 5
Size: 47 3834 2081
Fragments arranged by size:
3834 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5962
StyI C'CwwG_G
Cuts at: 513 1065 2004 2343 3401 513
Size: 552 939 339 1058 3074
Fragments arranged by size:
3074 1058 939 552 339
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3989 4006 4159 4344 4789 325
Size: 1255 2409 17 153 185 445 1498
Fragments arranged by size:
2409 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4208 5920 96
Size: 274 80 33 51 1443 2231 1712 138
Fragments arranged by size:
2231 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2989 3021 5922 5954 2989
Size: 32 2901 32 2997
Fragments arranged by size:
2997 2901 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2948 1316
Size: 1632 4330
XbaI T'CTAG_A
Cuts at: 3298 3298
Size: 5962
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2870 2870
Size: 5962
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5962
XmaI C'CCGG_G
Cuts at: 1870 2814 3287 1870
Size: 944 473 4545
Fragments arranged by size:
4545 944 473
XmnI GAAnn'nnTTC
Cuts at: 4091 4091
Size: 5962
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2350 3770 276
Size: 53 83 186 936 816 1420 2468
Fragments arranged by size:
2468 1420 936 816 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FalI FspAI NruI NsiI PacI PfoI PspOMI
PspXI PsrI RsrII SacI SexAI SfiI SrfI SwaI