Restriction map for
pFN21AE2255
(Circular) (Six-base) MAPSORT of: pFN21AE2255.seq Check: 6499 from: 1 to: 5827
pFN21AE2255 5827 bp
With 182 enzymes: SgfI *
March 28, 2012 12:02 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3637 278
Size: 53 83 186 936 2101 2468
Fragments arranged by size:
2468 2101 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3170 1937
Size: 1233 4594
Acc65I G'GTAC_C
Cuts at: 3148 3148
Size: 5827
AclI AA'CG_TT
Cuts at: 1563 3954 4327 1563
Size: 2391 373 3063
Fragments arranged by size:
3063 2391 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2214 2959 3890 5274 5542 1632
Size: 582 745 931 1384 268 1917
Fragments arranged by size:
1917 1384 931 745 582 268
AfeI AGC'GCT
Cuts at: 2406 2406
Size: 5827
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5630
AflIII A'CryG_T
Cuts at: 1203 4726 5703 1203
Size: 3523 977 1327
Fragments arranged by size:
3523 1327 977
AgeI A'CCGG_T
Cuts at: 4658 4658
Size: 5827
AhdI GACnn_n'nnGTC
Cuts at: 4556 4556
Size: 5827
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5827
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5827
AlwNI CAG_nnn'CTG
Cuts at: 2601 2962 3570 5142 2601
Size: 361 608 1572 3286
Fragments arranged by size:
3286 1572 608 361
ApaI G_GGCC'C
Cuts at: 2479 2479
Size: 5827
ApaLI G'TGCA_C
Cuts at: 3884 5040 3884
Size: 1156 4671
ApoI r'AATT_y
Cuts at: 1493 2105 2173 3136 3362 3514 5623 1493
Size: 612 68 963 226 152 2109 1697
Fragments arranged by size:
2109 1697 963 612 226 152 68
AseI AT'TA_AT
Cuts at: 160 1032 4381 160
Size: 872 3349 1606
Fragments arranged by size:
3349 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5827
AvaI C'yCGr_G
Cuts at: 1870 1943 3072 3122 3152 1870
Size: 73 1129 50 30 4545
Fragments arranged by size:
4545 1129 73 50 30
BamHI G'GATC_C
Cuts at: 3157 3524 3157
Size: 367 5460
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2336 2453 2524 2647 2833
Size: 325 217 627 549 117 71 123 186
Cuts at: 2833 2969 3148 4603 618
Size: 136 179 1455 1842
Fragments arranged by size:
1842 1455 627 549 325 217 186 179
136 123 117 71
BanII G_rGCy'C
Cuts at: 1391 2218 2479 2952 3146 1391
Size: 827 261 473 194 4072
Fragments arranged by size:
4072 827 473 261 194
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2236 2530 2866 928
Size: 971 337 294 336 3889
Fragments arranged by size:
3889 971 337 336 294
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2286 2320 4018 4052 1903
Size: 34 50 34 265 34 1698 34 3678
Fragments arranged by size:
3678 1698 265 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3719 4929 3719
Size: 1210 4617
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5827
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2306 2492 4438
Size: 107 122 71 631 605 634 186 1946
Cuts at: 4438 136
Size: 1525
Fragments arranged by size:
1946 1525 634 631 605 186 122 107
71
BglII A'GATC_T
Cuts at: 5822 5822
Size: 5827
BlpI GC'TnA_GC
Cuts at: 2408 3244 2408
Size: 836 4991
Bme1580I G_kGCm'C
Cuts at: 1790 2209 2341 2479 2529 2652 2838 3888 5044
Size: 419 132 138 50 123 186 1050 1156
Cuts at: 5044 1790
Size: 2573
Fragments arranged by size:
2573 1156 1050 419 186 138 132 123
50
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5827
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2434 3558 4516
Size: 416 53 83 399 24 1017 1124 958
Cuts at: 4516 442
Size: 1753
Fragments arranged by size:
1753 1124 1017 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5827
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2357 2433 2357
Size: 76 5751
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4487 1239
Size: 3248 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3952 4817 5115 3952
Size: 865 298 4664
Fragments arranged by size:
4664 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4490 882
Size: 617 2991 2219
Fragments arranged by size:
2991 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3618 493
Size: 711 2414 2702
Fragments arranged by size:
2702 2414 711
BsaBI GATnn'nnATC
Cuts at: 5821 5821
Size: 5827
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2658 3634 4016 4651
Size: 53 83 186 936 1125 976 382 635
Cuts at: 4651 275
Size: 1451
Fragments arranged by size:
1451 1125 976 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2060 4260 4658 4932 5079 5490 2060
Size: 2200 398 274 147 411 2397
Fragments arranged by size:
2397 2200 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2055 2085 2268 2298 3003
Size: 30 165 30 652 30 183 30 705
Cuts at: 3003 3033 1178
Size: 30 3972
Fragments arranged by size:
3972 705 652 183 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5827
BseYI C'CCAG_C
Cuts at: 2167 2591 2605 3015 5030 5526 2167
Size: 424 14 410 2015 496 2468
Fragments arranged by size:
2468 2015 496 424 410 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5254
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2990 3277 4038 5066 1540
Size: 275 186 989 287 761 1028 2301
Fragments arranged by size:
2301 1028 989 761 287 275 186
BsiHKAI G_wGCw'C
Cuts at: 3888 3973 5044 3888
Size: 85 1071 4671
Fragments arranged by size:
4671 1071 85
BsmI GAATG_Cn'
Cuts at: 3338 3431 3338
Size: 93 5734
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2209 2218 2341 2479 2529 2652 2838
Size: 399 419 9 123 138 50 123 186
Cuts at: 2838 2952 3146 3888 3973 5044 1391
Size: 114 194 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 419 399 194 186 138
123 123 114 85 50 9
BspEI T'CCGG_A
Cuts at: 2060 5490 2060
Size: 3430 2397
BspHI T'CATG_A
Cuts at: 3716 5446 3716
Size: 1730 4097
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2586 2685 3031 3182 844
Size: 1742 99 346 151 3489
Fragments arranged by size:
3489 1742 346 151 99
BsrBI CCG'CTC
Cuts at: 1394 1823 2742 3714 1394
Size: 429 919 972 3507
Fragments arranged by size:
3507 972 919 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4322 4496 65
Size: 1183 3074 174 1396
Fragments arranged by size:
3074 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4471 4658 1700
Size: 110 2661 187 2869
Fragments arranged by size:
2869 2661 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5827
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5827
BssSI C'ACGA_G
Cuts at: 3887 4899 3887
Size: 1012 4815
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2910 794
Size: 2116 3711
BstBI TT'CG_AA
Cuts at: 4631 4631
Size: 5827
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5827
BstXI CCAn_nnnn'nTGG
Cuts at: 2794 2794
Size: 5827
BstYI r'GATC_y
Cuts at: 1972 2980 3157 3524 3909 3926 5378 5464 5476
Size: 1008 177 367 385 17 1452 86 12
Cuts at: 5476 5822 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1008 385 367 346 177 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2117 2939 2117
Size: 822 5005
BtgI C'CryG_G
Cuts at: 513 1065 2436 513
Size: 552 1371 3904
Fragments arranged by size:
3904 1371 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5088
Fragments arranged by size:
5088 683 56
BtsI GCAGTG_nn'
Cuts at: 2074 2502 3343 4137 4157 2074
Size: 428 841 794 20 3744
Fragments arranged by size:
3744 841 794 428 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5827
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5792
DraI TTT'AAA
Cuts at: 3131 3131
Size: 5827
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2240 2729 4834 809
Size: 507 924 489 2105 1802
Fragments arranged by size:
2105 1802 924 507 489
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2361 2433 3274
Size: 54 1425 50 275 18 531 72 841
Cuts at: 3274 4163 5792 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 841 531 275 72 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3274 1537
Size: 275 1462 4090
Fragments arranged by size:
4090 1462 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2474 3757 1353
Size: 1121 1283 3423
Fragments arranged by size:
3423 1283 1121
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2276 3024 4414 4788 4934 387
Size: 1064 138 687 748 1390 374 146 1280
Fragments arranged by size:
1390 1280 1064 748 687 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2214 2959 3890 4487 5274 5542 1239
Size: 393 582 745 931 597 787 268 1524
Fragments arranged by size:
1524 931 787 745 597 582 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3038 3038
Size: 5827
EcoO109I rG'GnC_Cy
Cuts at: 2610 2610
Size: 5827
EcoRI G'AATT_C
Cuts at: 3136 3514 3136
Size: 378 5449
FspI TGC'GCA
Cuts at: 4333 4333
Size: 5827
HaeII r_GCGC'y
Cuts at: 1678 2408 4974 1678
Size: 730 2566 2531
Fragments arranged by size:
2566 2531 730
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2262 2294 2972 3004 4468
Size: 32 135 32 690 32 678 32 1464
Cuts at: 4468 4500 4542 4574 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1464 690 678 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3171 3417 669
Size: 988 281 1233 246 3079
Fragments arranged by size:
3079 1233 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5827
HpaI GTT'AAC
Cuts at: 3417 3417
Size: 5827
KpnI G_GTAC'C
Cuts at: 3152 3152
Size: 5827
MfeI C'AATT_G
Cuts at: 3426 3426
Size: 5827
MluI A'CGCG_T
Cuts at: 5703 5703
Size: 5827
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4941 5125 1305
Size: 3636 184 2007
Fragments arranged by size:
3636 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2435 10
Size: 54 1425 343 603 3402
Fragments arranged by size:
3402 1425 603 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2756 3785 4144 4303 518
Size: 648 1590 1029 359 159 2042
Fragments arranged by size:
2042 1590 1029 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2534 2620 2701 3243 3920 5068 5313 1606
Size: 928 86 81 542 677 1148 245 2120
Fragments arranged by size:
2120 1148 928 677 542 245 86 81
NcoI C'CATG_G
Cuts at: 513 1065 2436 513
Size: 552 1371 3904
Fragments arranged by size:
3904 1371 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5827
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5827
NotI GC'GGCC_GC
Cuts at: 3274 3274
Size: 5827
NspI r_CATG'y
Cuts at: 1132 1229 3185 4730 1132
Size: 97 1956 1545 2229
Fragments arranged by size:
2229 1956 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3004 1348
Size: 1656 4171
PciI A'CATG_T
Cuts at: 4726 4726
Size: 5827
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5827
PmeI GTTT'AAAC
Cuts at: 3131 3131
Size: 5827
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3896 3928 5409 5441 3896
Size: 32 1481 32 4282
Fragments arranged by size:
4282 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2870 1613
Size: 1257 4570
PsiI TTA'TAA
Cuts at: 3397 3397
Size: 5827
PspOMI G'GGCC_C
Cuts at: 2475 2475
Size: 5827
PstI C_TGCA'G
Cuts at: 830 2625 2700 3179 830
Size: 1795 75 479 3478
Fragments arranged by size:
3478 1795 479 75
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5827
PvuII CAG'CTG
Cuts at: 2534 2620 2534
Size: 86 5741
SalI G'TCGA_C
Cuts at: 1936 3169 1936
Size: 1233 4594
SbfI CC_TGCA'GG
Cuts at: 3179 3179
Size: 5827
ScaI AGT'ACT
Cuts at: 2848 4075 5787 2848
Size: 1227 1712 2888
Fragments arranged by size:
2888 1712 1227
SexAI A'CCwGG_T
Cuts at: 2829 2829
Size: 5827
SfcI C'TryA_G
Cuts at: 826 1045 2621 2696 3175 4310 4991 5182 826
Size: 219 1576 75 479 1135 681 191 1471
Fragments arranged by size:
1576 1471 1135 681 479 219 191 75
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5827
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5827
SmaI CCC'GGG
Cuts at: 1872 3074 3124 3154 1872
Size: 1202 50 30 4545
Fragments arranged by size:
4545 1202 50 30
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3931 4832 5094 820
Size: 197 926 1988 901 262 1553
Fragments arranged by size:
1988 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3618 493
Size: 3125 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5827
SphI G_CATG'C
Cuts at: 1132 3185 1132
Size: 2053 3774
SspI AAT'ATT
Cuts at: 5 52 3751 5
Size: 47 3699 2081
Fragments arranged by size:
3699 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5827
StyI C'CwwG_G
Cuts at: 513 1065 2436 2652 3266 513
Size: 552 1371 216 614 3074
Fragments arranged by size:
3074 1371 614 552 216
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2050 2416 2471 3007 3854 3871 4024
Size: 1255 470 366 55 536 847 17 153
Cuts at: 4024 4209 4654 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 847 536 470 445 366 185
153 55 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2846 2928 4073
Size: 274 80 33 51 1443 869 82 1145
Cuts at: 4073 5785 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1145 869 274 138 82 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2055 2087 2268 2300 3003 3035 5787 5819 2055
Size: 32 181 32 703 32 2752 32 2063
Fragments arranged by size:
2752 2063 703 181 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5827
XbaI T'CTAG_A
Cuts at: 3163 3163
Size: 5827
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2164 2164
Size: 5827
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5827
XmaI C'CCGG_G
Cuts at: 1870 3072 3122 3152 1870
Size: 1202 50 30 4545
Fragments arranged by size:
4545 1202 50 30
XmnI GAAnn'nnTTC
Cuts at: 3956 3956
Size: 5827
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3635 276
Size: 53 83 186 936 2101 2468
Fragments arranged by size:
2468 2101 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI
SacII SanDI SapI SfiI SfoI SrfI SwaI