Restriction map for
pFN21AE2240
(Circular) (Six-base) MAPSORT of: pFN21AE2240.seq Check: 3270 from: 1 to: 4945
pFN21AE2240 4945 bp
With 182 enzymes: SgfI *
July 6, 2012 17:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2755 278
Size: 53 83 186 936 1219 2468
Fragments arranged by size:
2468 1219 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2288 1937
Size: 351 4594
Acc65I G'GTAC_C
Cuts at: 2266 2266
Size: 4945
AclI AA'CG_TT
Cuts at: 1563 3072 3445 1563
Size: 1509 373 3063
Fragments arranged by size:
3063 1509 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3008 4392 4660 1632
Size: 1376 1384 268 1917
Fragments arranged by size:
1917 1384 1376 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4748
AflIII A'CryG_T
Cuts at: 1203 3844 4821 1203
Size: 2641 977 1327
Fragments arranged by size:
2641 1327 977
AgeI A'CCGG_T
Cuts at: 3776 3776
Size: 4945
AhdI GACnn_n'nnGTC
Cuts at: 3674 3674
Size: 4945
AleI CACnn'nnGTG
Cuts at: 1166 2171 1166
Size: 1005 3940
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 4945
AlwNI CAG_nnn'CTG
Cuts at: 2688 4260 2688
Size: 1572 3373
ApaLI G'TGCA_C
Cuts at: 3002 4158 3002
Size: 1156 3789
ApoI r'AATT_y
Cuts at: 1493 2254 2480 2632 4741 1493
Size: 761 226 152 2109 1697
Fragments arranged by size:
2109 1697 761 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3499 160
Size: 872 2467 1606
Fragments arranged by size:
2467 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4945
AvaI C'yCGr_G
Cuts at: 1870 1943 2030 2270 1870
Size: 73 87 240 4545
Fragments arranged by size:
4545 240 87 73
BamHI G'GATC_C
Cuts at: 2275 2642 2275
Size: 367 4578
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2183 2266 3721 618
Size: 325 217 627 396 83 1455 1842
Fragments arranged by size:
1842 1455 627 396 325 217 83
BanII G_rGCy'C
Cuts at: 1391 2264 1391
Size: 873 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 3974
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3136 3170 1903
Size: 34 1199 34 3678
Fragments arranged by size:
3678 1199 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2837 4047 2837
Size: 1210 3735
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4945
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3556 136
Size: 107 122 71 631 605 1884 1525
Fragments arranged by size:
1884 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4940 4940
Size: 4945
BlpI GC'TnA_GC
Cuts at: 2362 2362
Size: 4945
Bme1580I G_kGCm'C
Cuts at: 1790 2188 3006 4162 1790
Size: 398 818 1156 2573
Fragments arranged by size:
2573 1156 818 398
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 4945
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2676 3634 442
Size: 416 53 83 399 24 1259 958 1753
Fragments arranged by size:
1753 1259 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 4945
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3605 1239
Size: 2366 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3070 3935 4233 3070
Size: 865 298 3782
Fragments arranged by size:
3782 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2221 3608 882
Size: 617 722 1387 2219
Fragments arranged by size:
2219 1387 722 617
BsaAI yAC'GTr
Cuts at: 493 1204 2736 493
Size: 711 1532 2702
Fragments arranged by size:
2702 1532 711
BsaBI GATnn'nnATC
Cuts at: 4939 4939
Size: 4945
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2752 3134 3769 275
Size: 53 83 186 936 1219 382 635 1451
Fragments arranged by size:
1451 1219 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3378 3776 4050 4197 4608 3378
Size: 398 274 147 411 3715
Fragments arranged by size:
3715 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4720
Fragments arranged by size:
4720 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2104 1186
Size: 918 4027
BseYI C'CCAG_C
Cuts at: 2035 4148 4644 2035
Size: 2113 496 2336
Fragments arranged by size:
2336 2113 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4372
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2395 3156 4184 1540
Size: 275 186 394 761 1028 2301
Fragments arranged by size:
2301 1028 761 394 275 186
BsiHKAI G_wGCw'C
Cuts at: 3006 3091 4162 3006
Size: 85 1071 3789
Fragments arranged by size:
3789 1071 85
BsmI GAATG_Cn'
Cuts at: 2456 2549 2456
Size: 93 4852
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2188 2264 3006 3091 4162 1391
Size: 399 398 76 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 398 85 76
BspEI T'CCGG_A
Cuts at: 4608 4608
Size: 4945
BspHI T'CATG_A
Cuts at: 2834 4564 2834
Size: 1730 3215
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2300 844
Size: 1456 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2832 1394
Size: 429 1009 3507
Fragments arranged by size:
3507 1009 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2022 3440 3614 65
Size: 1183 774 1418 174 1396
Fragments arranged by size:
1418 1396 1183 774 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3589 3776 1700
Size: 110 1779 187 2869
Fragments arranged by size:
2869 1779 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 4945
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 4945
BssSI C'ACGA_G
Cuts at: 3005 4017 3005
Size: 1012 3933
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 4945
BstBI TT'CG_AA
Cuts at: 3749 3749
Size: 4945
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 4945
BstXI CCAn_nnnn'nTGG
Cuts at: 2173 2173
Size: 4945
BstYI r'GATC_y
Cuts at: 1972 2275 2642 3027 3044 4496 4582 4594 4940
Size: 303 367 385 17 1452 86 12 346
Cuts at: 4940 1972
Size: 1977
Fragments arranged by size:
1977 1452 385 367 346 303 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3454
Fragments arranged by size:
3454 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4206
Fragments arranged by size:
4206 683 56
BtsI GCAGTG_nn'
Cuts at: 2054 2461 3255 3275 2054
Size: 407 794 20 3724
Fragments arranged by size:
3724 794 407 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 4945
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4910
DraI TTT'AAA
Cuts at: 2249 2249
Size: 4945
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3952 809
Size: 507 2636 1802
Fragments arranged by size:
2636 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2022 2392 3281
Size: 54 1425 50 275 18 192 370 889
Cuts at: 3281 4910 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 370 275 192 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2392 1537
Size: 275 580 4090
Fragments arranged by size:
4090 580 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2213 2232 2875 1353
Size: 860 19 643 3423
Fragments arranged by size:
3423 860 643 19
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3532 3906 4052 387
Size: 1064 138 1943 374 146 1280
Fragments arranged by size:
1943 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3008 3605 4392 4660 1239
Size: 393 1376 597 787 268 1524
Fragments arranged by size:
1524 1376 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2254 2632 2254
Size: 378 4567
FspI TGC'GCA
Cuts at: 3451 3451
Size: 4945
HaeII r_GCGC'y
Cuts at: 1678 4092 1678
Size: 2414 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3586 3618 3660 3692 1373
Size: 32 135 32 2014 32 42 32 2626
Fragments arranged by size:
2626 2014 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2196 2289 2535 669
Size: 988 281 258 93 246 3079
Fragments arranged by size:
3079 988 281 258 246 93
HindIII A'AGCT_T
Cuts at: 748 748
Size: 4945
HpaI GTT'AAC
Cuts at: 2535 2535
Size: 4945
KpnI G_GTAC'C
Cuts at: 2270 2270
Size: 4945
MfeI C'AATT_G
Cuts at: 2544 2544
Size: 4945
MluI A'CGCG_T
Cuts at: 4821 4821
Size: 4945
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2154 2177 4059 4243 1305
Size: 849 23 1882 184 2007
Fragments arranged by size:
2007 1882 849 184 23
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2024 10
Size: 54 1425 343 192 2931
Fragments arranged by size:
2931 1425 343 192 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2171 2903 3262 3421 518
Size: 648 1005 732 359 159 2042
Fragments arranged by size:
2042 1005 732 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2361 3038 4186 4431 1606
Size: 755 677 1148 245 2120
Fragments arranged by size:
2120 1148 755 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3454
Fragments arranged by size:
3454 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 4945
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 4945
NotI GC'GGCC_GC
Cuts at: 2392 2392
Size: 4945
NspI r_CATG'y
Cuts at: 1132 1229 2303 3848 1132
Size: 97 1074 1545 2229
Fragments arranged by size:
2229 1545 1074 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 4945
PciI A'CATG_T
Cuts at: 3844 3844
Size: 4945
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 4945
PmeI GTTT'AAAC
Cuts at: 2249 2249
Size: 4945
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3014 3046 4527 4559 3014
Size: 32 1481 32 3400
Fragments arranged by size:
3400 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 4945
PsiI TTA'TAA
Cuts at: 2515 2515
Size: 4945
PstI C_TGCA'G
Cuts at: 830 2193 2297 830
Size: 1363 104 3478
Fragments arranged by size:
3478 1363 104
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 4945
SalI G'TCGA_C
Cuts at: 1936 2287 1936
Size: 351 4594
SbfI CC_TGCA'GG
Cuts at: 2297 2297
Size: 4945
ScaI AGT'ACT
Cuts at: 2125 3193 4905 2125
Size: 1068 1712 2165
Fragments arranged by size:
2165 1712 1068
SfcI C'TryA_G
Cuts at: 826 1045 2189 2293 3428 4109 4300 826
Size: 219 1144 104 1135 681 191 1471
Fragments arranged by size:
1471 1144 1135 681 219 191 104
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4945
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 4945
SmaI CCC'GGG
Cuts at: 1872 2272 1872
Size: 400 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3049 3950 4212 820
Size: 197 926 1106 901 262 1553
Fragments arranged by size:
1553 1106 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2736 493
Size: 2243 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 4945
SphI G_CATG'C
Cuts at: 1132 2303 1132
Size: 1171 3774
SspI AAT'ATT
Cuts at: 5 52 2869 5
Size: 47 2817 2081
Fragments arranged by size:
2817 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 4945
StyI C'CwwG_G
Cuts at: 513 1065 2004 2169 2384 513
Size: 552 939 165 215 3074
Fragments arranged by size:
3074 939 552 215 165
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2972 2989 3142 3327 3772 325
Size: 1255 1392 17 153 185 445 1498
Fragments arranged by size:
1498 1392 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2123 3191 4903
Size: 274 80 33 51 1443 146 1068 1712
Cuts at: 4903 96
Size: 138
Fragments arranged by size:
1712 1443 1068 274 146 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4905 4937 4905
Size: 32 4913
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 4945
XbaI T'CTAG_A
Cuts at: 2281 2281
Size: 4945
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2032 2137 2170 2209 2032
Size: 105 33 39 4768
Fragments arranged by size:
4768 105 39 33
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 4945
XmaI C'CCGG_G
Cuts at: 1870 2270 1870
Size: 400 4545
XmnI GAAnn'nnTTC
Cuts at: 3074 3074
Size: 4945
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2753 276
Size: 53 83 186 936 1219 2468
Fragments arranged by size:
2468 1219 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI