Restriction map for
pFN21AE2237
(Circular) (Six-base) MAPSORT of: pFN21AE2237.seq Check: 4001 from: 1 to: 6784
pFN21AE2237 6784 bp
With 182 enzymes: SgfI *
August 16, 2021 15:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2812 2812
Size: 6784
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2015 4594 278
Size: 53 83 186 936 479 2579 2468
Fragments arranged by size:
2579 2468 936 479 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4127 1937
Size: 2190 4594
Acc65I G'GTAC_C
Cuts at: 4105 4105
Size: 6784
AclI AA'CG_TT
Cuts at: 1563 4911 5284 1563
Size: 3348 373 3063
Fragments arranged by size:
3348 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2705 2904 3077 3762 4847 6231 6499 1632
Size: 1073 199 173 685 1085 1384 268 1917
Fragments arranged by size:
1917 1384 1085 1073 685 268 199 173
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6587
AflIII A'CryG_T
Cuts at: 1203 5683 6660 1203
Size: 4480 977 1327
Fragments arranged by size:
4480 1327 977
AgeI A'CCGG_T
Cuts at: 5615 5615
Size: 6784
AhdI GACnn_n'nnGTC
Cuts at: 5513 5513
Size: 6784
AleI CACnn'nnGTG
Cuts at: 1166 3288 1166
Size: 2122 4662
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6784
AlwNI CAG_nnn'CTG
Cuts at: 3355 3941 4527 6099 3355
Size: 586 586 1572 4040
Fragments arranged by size:
4040 1572 586 586
ApaI G_GGCC'C
Cuts at: 2984 3326 3621 2984
Size: 342 295 6147
Fragments arranged by size:
6147 342 295
ApaLI G'TGCA_C
Cuts at: 4841 5997 4841
Size: 1156 5628
ApoI r'AATT_y
Cuts at: 1493 2066 4093 4319 4471 6580 1493
Size: 573 2027 226 152 2109 1697
Fragments arranged by size:
2109 2027 1697 573 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5338 160
Size: 872 4306 1606
Fragments arranged by size:
4306 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6784
AvaI C'yCGr_G
Cuts at: 1870 1943 4109 1870
Size: 73 2166 4545
Fragments arranged by size:
4545 2166 73
BamHI G'GATC_C
Cuts at: 3724 3790 4114 4481 3724
Size: 66 324 367 6027
Fragments arranged by size:
6027 367 324 66
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2301 3239 4105 5560 618
Size: 325 217 627 514 938 866 1455 1842
Fragments arranged by size:
1842 1455 938 866 627 514 325 217
BanII G_rGCy'C
Cuts at: 1391 2198 2264 2825 2984 3101 3326 3621 3704
Size: 807 66 561 159 117 225 295 83
Cuts at: 3704 4103 1391
Size: 399 4072
Fragments arranged by size:
4072 807 561 399 295 225 159 117
83 66
BbeI G_GCGC'C
Cuts at: 3243 3243
Size: 6784
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3387 928
Size: 971 1488 4325
Fragments arranged by size:
4325 1488 971
BbvCI CC'TCA_GC
Cuts at: 3081 3299 3081
Size: 218 6566
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2465 2499 4975 5009 1903
Size: 34 528 34 2476 34 3678
Fragments arranged by size:
3678 2476 528 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2554 3415 4676 5886 2554
Size: 861 1261 1210 3452
Fragments arranged by size:
3452 1261 1210 861
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6784
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3625 5395 136
Size: 107 122 71 631 605 1953 1770 1525
Fragments arranged by size:
1953 1770 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6779 6779
Size: 6784
BlpI GC'TnA_GC
Cuts at: 4201 4201
Size: 6784
Bme1580I G_kGCm'C
Cuts at: 1790 2984 3297 3326 3621 4033 4845 6001 1790
Size: 1194 313 29 295 412 812 1156 2573
Fragments arranged by size:
2573 1194 1156 812 412 313 295 29
BmgBI CAC'GTC
Cuts at: 1318 2606 1318
Size: 1288 5496
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2544 3615 4001
Size: 416 53 83 399 24 1127 1071 386
Cuts at: 4001 4515 5473 442
Size: 514 958 1753
Fragments arranged by size:
1753 1127 1071 958 514 416 399 386
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6784
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3096 3096
Size: 6784
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3044 3701 3861 5444 1239
Size: 1805 657 160 1583 2579
Fragments arranged by size:
2579 1805 1583 657 160
Bpu10I CC'TnA_GC
Cuts at: 3044 3071 3081 3299 3841 3044
Size: 27 10 218 542 5987
Fragments arranged by size:
5987 542 218 27 10
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4909 5774 6072 4909
Size: 865 298 5621
Fragments arranged by size:
5621 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2217 5447 882
Size: 617 718 3230 2219
Fragments arranged by size:
3230 2219 718 617
BsaAI yAC'GTr
Cuts at: 493 1204 4575 493
Size: 711 3371 2702
Fragments arranged by size:
3371 2702 711
BsaBI GATnn'nnATC
Cuts at: 2141 6778 2141
Size: 4637 2147
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2012 3240 3333 3411
Size: 53 83 186 936 479 1228 93 78
Cuts at: 3411 4591 4973 5608 275
Size: 1180 382 635 1451
Fragments arranged by size:
1451 1228 1180 936 635 479 382 186
93 83 78 53
BsaWI w'CCGG_w
Cuts at: 5217 5615 5889 6036 6447 5217
Size: 398 274 147 411 5554
Fragments arranged by size:
5554 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2132 2162 2909 2939 3211
Size: 30 165 30 729 30 747 30 272
Cuts at: 3211 3241 1178
Size: 30 4721
Fragments arranged by size:
4721 747 729 272 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2123 2141 2403 2669 3118 3187 3190 3193
Size: 937 18 262 266 449 69 3 3
Cuts at: 3193 3202 3205 3862 4070 1186
Size: 9 3 657 208 3900
Fragments arranged by size:
3900 937 657 449 266 262 208 69
18 9 3 3 3
BseYI C'CCAG_C
Cuts at: 2585 2597 3087 3325 3495 5987 6483 2585
Size: 12 490 238 170 2492 496 2886
Fragments arranged by size:
2886 2492 496 490 238 170 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2345 2481 2831 3575 1163
Size: 573 609 136 350 744 4372
Fragments arranged by size:
4372 744 609 573 350 136
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4234 4995 6023 1540
Size: 275 186 2233 761 1028 2301
Fragments arranged by size:
2301 2233 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2198 2297 3187 4845 4930 6001 2198
Size: 99 890 1658 85 1071 2981
Fragments arranged by size:
2981 1658 1071 890 99 85
BsmI GAATG_Cn'
Cuts at: 4295 4388 4295
Size: 93 6691
BsmBI CGTCTCn'nnnn_
Cuts at: 2105 2105
Size: 6784
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2198 2264 2297 2825 2984 3101 3187
Size: 399 408 66 33 528 159 117 86
Cuts at: 3187 3297 3326 3621 3704 4033 4103 4845 4930
Size: 110 29 295 83 329 70 742 85
Cuts at: 4930 6001 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 528 408 399 329 295
159 117 110 86 85 83 70 66
33 29
BspEI T'CCGG_A
Cuts at: 6447 6447
Size: 6784
BspHI T'CATG_A
Cuts at: 4673 6403 4673
Size: 1730 5054
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2653 2812 2853 4139 844
Size: 1809 159 41 1286 3489
Fragments arranged by size:
3489 1809 1286 159 41
BsrBI CCG'CTC
Cuts at: 1394 1823 4671 1394
Size: 429 2848 3507
Fragments arranged by size:
3507 2848 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5279 5453 65
Size: 1183 4031 174 1396
Fragments arranged by size:
4031 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3648 4074 5428 5615 1700
Size: 110 1838 426 1354 187 2869
Fragments arranged by size:
2869 1838 1354 426 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6784
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6784
BssSI C'ACGA_G
Cuts at: 4844 5856 4844
Size: 1012 5772
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6784
BstBI TT'CG_AA
Cuts at: 5588 5588
Size: 6784
BstEII G'GTnAC_C
Cuts at: 1184 3450 1184
Size: 2266 4518
BstXI CCAn_nnnn'nTGG
Cuts at: 2875 3163 2875
Size: 288 6496
BstYI r'GATC_y
Cuts at: 1972 2332 3724 3790 4114 4481 4866 4883 6335
Size: 360 1392 66 324 367 385 17 1452
Cuts at: 6335 6421 6433 6779 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1392 385 367 360 346 324
86 66 17 12
Bsu36I CC'TnA_GG
Cuts at: 3562 3562
Size: 6784
BtgI C'CryG_G
Cuts at: 513 1065 2004 2040 2305 2690 3835 513
Size: 552 939 36 265 385 1145 3462
Fragments arranged by size:
3462 1145 939 552 385 265 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6045
Fragments arranged by size:
6045 683 56
BtsI GCAGTG_nn'
Cuts at: 2861 4075 4300 5094 5114 2861
Size: 1214 225 794 20 4531
Fragments arranged by size:
4531 1214 794 225 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6784
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6749
DraI TTT'AAA
Cuts at: 4088 4088
Size: 6784
DraIII CAC_nnn'GTG
Cuts at: 3182 3182
Size: 6784
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5791 809
Size: 507 4475 1802
Fragments arranged by size:
4475 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2037 2247 3650
Size: 54 1425 50 275 18 207 210 1403
Cuts at: 3650 3909 4231 5120 6749 8
Size: 259 322 889 1629 43
Fragments arranged by size:
1629 1425 1403 889 322 275 259 210
207 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4231 1537
Size: 275 2419 4090
Fragments arranged by size:
4090 2419 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2725 2880 3211 4714 1353
Size: 1372 155 331 1503 3423
Fragments arranged by size:
3423 1503 1372 331 155
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5371 5745 5891 387
Size: 1064 138 3782 374 146 1280
Fragments arranged by size:
3782 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2196 2196
Size: 6784
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2705 2904 3044 3077 3701 3762 3861
Size: 393 1073 199 140 33 624 61 99
Cuts at: 3861 4847 5444 6231 6499 1239
Size: 986 597 787 268 1524
Fragments arranged by size:
1524 1073 986 787 624 597 393 268
199 140 99 61 33
EcoO109I rG'GnC_Cy
Cuts at: 2573 3270 3342 3746 3845 2573
Size: 697 72 404 99 5512
Fragments arranged by size:
5512 697 404 99 72
EcoRI G'AATT_C
Cuts at: 4093 4471 4093
Size: 378 6406
FspI TGC'GCA
Cuts at: 3980 5290 3980
Size: 1310 5474
HaeII r_GCGC'y
Cuts at: 1678 3243 5931 1678
Size: 1565 2688 2531
Fragments arranged by size:
2688 2531 1565
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2132 2164 3689 3721 5425
Size: 32 135 32 560 32 1525 32 1704
Cuts at: 5425 5457 5499 5531 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1704 1525 560 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2227 4128 4374 669
Size: 988 281 289 1901 246 3079
Fragments arranged by size:
3079 1901 988 289 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6784
HpaI GTT'AAC
Cuts at: 2227 4374 2227
Size: 2147 4637
KasI G'GCGC_C
Cuts at: 3239 3239
Size: 6784
KpnI G_GTAC'C
Cuts at: 4109 4109
Size: 6784
MfeI C'AATT_G
Cuts at: 4383 4383
Size: 6784
MluI A'CGCG_T
Cuts at: 6660 6660
Size: 6784
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5898 6082 1305
Size: 4593 184 2007
Fragments arranged by size:
4593 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4962
Fragments arranged by size:
4962 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2205 2535 3288 4742 5101 5260 518
Size: 648 1039 330 753 1454 359 159 2042
Fragments arranged by size:
2042 1454 1039 753 648 359 330 159
MspA1I CmG'CkG
Cuts at: 1606 3355 3448 3941 4200 4877 6025 6270 1606
Size: 1749 93 493 259 677 1148 245 2120
Fragments arranged by size:
2120 1749 1148 677 493 259 245 93
NarI GG'CG_CC
Cuts at: 3240 3240
Size: 6784
NcoI C'CATG_G
Cuts at: 513 1065 2004 2305 2690 3835 513
Size: 552 939 301 385 1145 3462
Fragments arranged by size:
3462 1145 939 552 385 301
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6784
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6784
NotI GC'GGCC_GC
Cuts at: 4231 4231
Size: 6784
NspI r_CATG'y
Cuts at: 1132 1229 4015 4142 5687 1132
Size: 97 2786 127 1545 2229
Fragments arranged by size:
2786 2229 1545 127 97
PasI CC'CwG_GG
Cuts at: 1348 3163 3266 1348
Size: 1815 103 4866
Fragments arranged by size:
4866 1815 103
PciI A'CATG_T
Cuts at: 5683 5683
Size: 6784
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2311 3779 1108
Size: 1203 1468 4113
Fragments arranged by size:
4113 1468 1203
PmeI GTTT'AAAC
Cuts at: 4088 4088
Size: 6784
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4853 4885 6366 6398 4853
Size: 32 1481 32 5239
Fragments arranged by size:
5239 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3342 3342
Size: 6784
PshAI GACnn'nnGTC
Cuts at: 1613 2731 1613
Size: 1118 5666
PsiI TTA'TAA
Cuts at: 4354 4354
Size: 6784
PspOMI G'GGCC_C
Cuts at: 2980 3322 3617 2980
Size: 342 295 6147
Fragments arranged by size:
6147 342 295
PstI C_TGCA'G
Cuts at: 830 2091 2467 3036 4136 830
Size: 1261 376 569 1100 3478
Fragments arranged by size:
3478 1261 1100 569 376
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6784
PvuII CAG'CTG
Cuts at: 3355 3941 3355
Size: 586 6198
SacI G_AGCT'C
Cuts at: 2198 2198
Size: 6784
SalI G'TCGA_C
Cuts at: 1936 4126 1936
Size: 2190 4594
SbfI CC_TGCA'GG
Cuts at: 4136 4136
Size: 6784
ScaI AGT'ACT
Cuts at: 5032 6744 5032
Size: 1712 5072
SfcI C'TryA_G
Cuts at: 826 1045 2087 2392 2463 3032 3518 4132 5267
Size: 219 1042 305 71 569 486 614 1135
Cuts at: 5267 5948 6139 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1042 681 614 569 486 305
219 191 71
SfoI GGC'GCC
Cuts at: 3241 3241
Size: 6784
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6784
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6784
SmaI CCC'GGG
Cuts at: 1872 4111 1872
Size: 2239 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4888 5789 6051 820
Size: 197 926 2945 901 262 1553
Fragments arranged by size:
2945 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4575 493
Size: 4082 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6784
SphI G_CATG'C
Cuts at: 1132 4142 1132
Size: 3010 3774
SspI AAT'ATT
Cuts at: 5 52 4708 5
Size: 47 4656 2081
Fragments arranged by size:
4656 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6784
StyI C'CwwG_G
Cuts at: 513 1065 2004 2305 2690 3132 3835 4223 513
Size: 552 939 301 385 442 703 388 3074
Fragments arranged by size:
3074 939 703 552 442 388 385 301
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2721 3039 3400 3767 4811 4828 4981
Size: 1255 1141 318 361 367 1044 17 153
Cuts at: 4981 5166 5611 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1141 1044 445 367 361 318
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3145 5030 6742
Size: 274 80 33 51 1443 1168 1885 1712
Cuts at: 6742 96
Size: 138
Fragments arranged by size:
1885 1712 1443 1168 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3211 3243 6744 6776 3211
Size: 32 3501 32 3219
Fragments arranged by size:
3501 3219 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6784
XbaI T'CTAG_A
Cuts at: 4120 4120
Size: 6784
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2237 2427 2950 3160 3718 2237
Size: 190 523 210 558 5303
Fragments arranged by size:
5303 558 523 210 190
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6784
XmaI C'CCGG_G
Cuts at: 1870 4109 1870
Size: 2239 4545
XmnI GAAnn'nnTTC
Cuts at: 4913 4913
Size: 6784
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2013 4592 276
Size: 53 83 186 936 479 2579 2468
Fragments arranged by size:
2579 2468 936 479 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I
EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfiI SrfI SwaI