Restriction map for
pFN21AE2236
(Circular) (Six-base) MAPSORT of: pFN21AE2236.seq Check: 4842 from: 1 to: 6133
pFN21AE2236 6133 bp
With 182 enzymes: SgfI *
July 6, 2012 17:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3943 278
Size: 53 83 186 936 2407 2468
Fragments arranged by size:
2468 2407 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3476 1937
Size: 1539 4594
Acc65I G'GTAC_C
Cuts at: 3454 3454
Size: 6133
AclI AA'CG_TT
Cuts at: 1563 4260 4633 1563
Size: 2697 373 3063
Fragments arranged by size:
3063 2697 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2284 2718 3334 4196 5580 5848 1632
Size: 652 434 616 862 1384 268 1917
Fragments arranged by size:
1917 1384 862 652 616 434 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5936
AflIII A'CryG_T
Cuts at: 1203 5032 6009 1203
Size: 3829 977 1327
Fragments arranged by size:
3829 1327 977
AgeI A'CCGG_T
Cuts at: 4964 4964
Size: 6133
AhdI GACnn_n'nnGTC
Cuts at: 4862 4862
Size: 6133
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6133
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6133
AlwNI CAG_nnn'CTG
Cuts at: 3876 5448 3876
Size: 1572 4561
ApaLI G'TGCA_C
Cuts at: 4190 5346 4190
Size: 1156 4977
ApoI r'AATT_y
Cuts at: 1493 2975 3442 3668 3820 5929 1493
Size: 1482 467 226 152 2109 1697
Fragments arranged by size:
2109 1697 1482 467 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4687 160
Size: 872 3655 1606
Fragments arranged by size:
3655 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6133
AvaI C'yCGr_G
Cuts at: 1870 1943 3458 1870
Size: 73 1515 4545
Fragments arranged by size:
4545 1515 73
BamHI G'GATC_C
Cuts at: 3463 3830 3463
Size: 367 5766
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2627 2824 3454 4909 618
Size: 325 217 627 840 197 630 1455 1842
Fragments arranged by size:
1842 1455 840 630 627 325 217 197
BanII G_rGCy'C
Cuts at: 1391 2679 2739 3452 1391
Size: 1288 60 713 4072
Fragments arranged by size:
4072 1288 713 60
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5162
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4324 4358 1903
Size: 34 2387 34 3678
Fragments arranged by size:
3678 2387 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4025 5235 4025
Size: 1210 4923
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6133
BfrBI ATG'CAT
Cuts at: 2011 2621 2011
Size: 610 5523
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4744 136
Size: 107 122 71 631 605 3072 1525
Fragments arranged by size:
3072 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6128 6128
Size: 6133
BlpI GC'TnA_GC
Cuts at: 3550 3550
Size: 6133
Bme1580I G_kGCm'C
Cuts at: 1790 2829 4194 5350 1790
Size: 1039 1365 1156 2573
Fragments arranged by size:
2573 1365 1156 1039
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6133
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2737 3864 4822
Size: 416 53 83 399 24 1320 1127 958
Cuts at: 4822 442
Size: 1753
Fragments arranged by size:
1753 1320 1127 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6133
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2104 2104
Size: 6133
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2273 2434 3247 4793 1239
Size: 1034 161 813 1546 2579
Fragments arranged by size:
2579 1546 1034 813 161
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4258 5123 5421 4258
Size: 865 298 4970
Fragments arranged by size:
4970 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4796 882
Size: 617 3297 2219
Fragments arranged by size:
3297 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3924 493
Size: 711 2720 2702
Fragments arranged by size:
2720 2702 711
BsaBI GATnn'nnATC
Cuts at: 6127 6127
Size: 6133
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3940 4322 4957 275
Size: 53 83 186 936 2407 382 635 1451
Fragments arranged by size:
2407 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4566 4964 5238 5385 5796 4566
Size: 398 274 147 411 4903
Fragments arranged by size:
4903 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2288 2318 1178
Size: 30 165 30 885 30 4993
Fragments arranged by size:
4993 885 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2096 1186
Size: 910 5223
BseYI C'CCAG_C
Cuts at: 2106 5336 5832 2106
Size: 3230 496 2407
Fragments arranged by size:
3230 2407 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5560
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3583 4344 5372 1540
Size: 275 186 1582 761 1028 2301
Fragments arranged by size:
2301 1582 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2739 4194 4279 5350 2739
Size: 1455 85 1071 3522
Fragments arranged by size:
3522 1455 1071 85
BsmI GAATG_Cn'
Cuts at: 2018 2205 2775 3644 3737 2018
Size: 187 570 869 93 4414
Fragments arranged by size:
4414 869 570 187 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2679 2739 2829 3452 4194 4279 5350
Size: 399 889 60 90 623 742 85 1071
Cuts at: 5350 1391
Size: 2174
Fragments arranged by size:
2174 1071 889 742 623 399 90 85
60
BspEI T'CCGG_A
Cuts at: 5796 5796
Size: 6133
BspHI T'CATG_A
Cuts at: 2653 4022 5752 2653
Size: 1369 1730 3034
Fragments arranged by size:
3034 1730 1369
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3101 3488 844
Size: 2257 387 3489
Fragments arranged by size:
3489 2257 387
BsrBI CCG'CTC
Cuts at: 1394 1823 4020 1394
Size: 429 2197 3507
Fragments arranged by size:
3507 2197 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3427 4628 4802 65
Size: 1183 2179 1201 174 1396
Fragments arranged by size:
2179 1396 1201 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4777 4964 1700
Size: 110 2967 187 2869
Fragments arranged by size:
2967 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6133
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6133
BssSI C'ACGA_G
Cuts at: 4193 5205 4193
Size: 1012 5121
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6133
BstBI TT'CG_AA
Cuts at: 4937 4937
Size: 6133
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6133
BstYI r'GATC_y
Cuts at: 1972 2098 2849 2951 3463 3830 4215 4232 5684
Size: 126 751 102 512 367 385 17 1452
Cuts at: 5684 5770 5782 6128 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 751 512 385 367 346 126
102 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3306 3306
Size: 6133
BtgI C'CryG_G
Cuts at: 513 1065 3252 513
Size: 552 2187 3394
Fragments arranged by size:
3394 2187 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5394
Fragments arranged by size:
5394 683 56
BtsI GCAGTG_nn'
Cuts at: 3649 4443 4463 3649
Size: 794 20 5319
Fragments arranged by size:
5319 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6133
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6098
DraI TTT'AAA
Cuts at: 2929 3067 3437 2929
Size: 138 370 5625
Fragments arranged by size:
5625 370 138
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5140 809
Size: 507 3824 1802
Fragments arranged by size:
3824 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2662 3580 4469
Size: 54 1425 50 275 18 832 918 889
Cuts at: 4469 6098 8
Size: 1629 43
Fragments arranged by size:
1629 1425 918 889 832 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3580 1537
Size: 275 1768 4090
Fragments arranged by size:
4090 1768 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2421 3012 4063 1353
Size: 1068 591 1051 3423
Fragments arranged by size:
3423 1068 1051 591
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2738 4720 5094 5240 387
Size: 1064 138 1149 1982 374 146 1280
Fragments arranged by size:
1982 1280 1149 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2737 2737
Size: 6133
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2273 2284 2434 2718 3247 3334 4196
Size: 393 641 11 150 284 529 87 862
Cuts at: 4196 4793 5580 5848 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 862 787 641 597 529 393 284
268 150 87 11
EcoNI CCTnn'n_nnAGG
Cuts at: 2138 2138
Size: 6133
EcoRI G'AATT_C
Cuts at: 3442 3820 3442
Size: 378 5755
FspI TGC'GCA
Cuts at: 2264 4639 2264
Size: 2375 3758
HaeII r_GCGC'y
Cuts at: 1678 5280 1678
Size: 3602 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2501 2533 4774 4806 4848
Size: 32 135 32 929 32 2241 32 42
Cuts at: 4848 4880 1373
Size: 32 2626
Fragments arranged by size:
2626 2241 929 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3040 3477 3723 669
Size: 988 281 1102 437 246 3079
Fragments arranged by size:
3079 1102 988 437 281 246
HindIII A'AGCT_T
Cuts at: 748 2700 748
Size: 1952 4181
HpaI GTT'AAC
Cuts at: 3040 3723 3040
Size: 683 5450
KpnI G_GTAC'C
Cuts at: 3458 3458
Size: 6133
MfeI C'AATT_G
Cuts at: 3732 3732
Size: 6133
MluI A'CGCG_T
Cuts at: 6009 6009
Size: 6133
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2115 2199 3173 5247 5431 1305
Size: 810 84 974 2074 184 2007
Fragments arranged by size:
2074 2007 974 810 184 84
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2664 10
Size: 54 1425 343 832 3479
Fragments arranged by size:
3479 1425 832 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2592 4091 4450 4609 518
Size: 648 1426 1499 359 159 2042
Fragments arranged by size:
2042 1499 1426 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3173 3215 3420 3549 4226 5374 5619 1606
Size: 1567 42 205 129 677 1148 245 2120
Fragments arranged by size:
2120 1567 1148 677 245 205 129 42
NcoI C'CATG_G
Cuts at: 513 1065 3252 513
Size: 552 2187 3394
Fragments arranged by size:
3394 2187 552
NdeI CA'TA_TG
Cuts at: 387 3031 387
Size: 2644 3489
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6133
NotI GC'GGCC_GC
Cuts at: 3580 3580
Size: 6133
NsiI A_TGCA'T
Cuts at: 2013 2623 2013
Size: 610 5523
NspI r_CATG'y
Cuts at: 1132 1229 2320 3491 5036 1132
Size: 97 1091 1171 1545 2229
Fragments arranged by size:
2229 1545 1171 1091 97
PasI CC'CwG_GG
Cuts at: 1348 2671 3136 1348
Size: 1323 465 4345
Fragments arranged by size:
4345 1323 465
PciI A'CATG_T
Cuts at: 5032 5032
Size: 6133
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2671 3136 3253 1108
Size: 1563 465 117 3988
Fragments arranged by size:
3988 1563 465 117
PfoI T'CCnGG_A
Cuts at: 3292 3292
Size: 6133
PmeI GTTT'AAAC
Cuts at: 3437 3437
Size: 6133
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4202 4234 5715 5747 4202
Size: 32 1481 32 4588
Fragments arranged by size:
4588 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2292 1613
Size: 679 5454
PsiI TTA'TAA
Cuts at: 3703 3703
Size: 6133
PstI C_TGCA'G
Cuts at: 830 3485 830
Size: 2655 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6133
PvuII CAG'CTG
Cuts at: 3173 3420 3173
Size: 247 5886
SacI G_AGCT'C
Cuts at: 2739 2739
Size: 6133
SalI G'TCGA_C
Cuts at: 1936 3475 1936
Size: 1539 4594
SbfI CC_TGCA'GG
Cuts at: 3485 3485
Size: 6133
ScaI AGT'ACT
Cuts at: 2126 4381 6093 2126
Size: 2255 1712 2166
Fragments arranged by size:
2255 2166 1712
SfcI C'TryA_G
Cuts at: 826 1045 3481 4616 5297 5488 826
Size: 219 2436 1135 681 191 1471
Fragments arranged by size:
2436 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6133
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6133
SmaI CCC'GGG
Cuts at: 1872 3460 1872
Size: 1588 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4237 5138 5400 820
Size: 197 926 2294 901 262 1553
Fragments arranged by size:
2294 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3924 493
Size: 3431 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6133
SphI G_CATG'C
Cuts at: 1132 2320 3491 1132
Size: 1188 1171 3774
Fragments arranged by size:
3774 1188 1171
SspI AAT'ATT
Cuts at: 5 52 4057 5
Size: 47 4005 2081
Fragments arranged by size:
4005 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6133
StyI C'CwwG_G
Cuts at: 513 1065 2194 2212 3252 3572 513
Size: 552 1129 18 1040 320 3074
Fragments arranged by size:
3074 1129 1040 552 320 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4160 4177 4330 4515 4960 325
Size: 1255 2580 17 153 185 445 1498
Fragments arranged by size:
2580 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2124 4379 6091
Size: 274 80 33 51 1443 147 2255 1712
Cuts at: 6091 96
Size: 138
Fragments arranged by size:
2255 1712 1443 274 147 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2288 2320 6093 6125 2288
Size: 32 3773 32 2296
Fragments arranged by size:
3773 2296 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6133
XbaI T'CTAG_A
Cuts at: 3469 3469
Size: 6133
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3388 3388
Size: 6133
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6133
XmaI C'CCGG_G
Cuts at: 1870 3458 1870
Size: 1588 4545
XmnI GAAnn'nnTTC
Cuts at: 4262 4262
Size: 6133
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3941 276
Size: 53 83 186 936 2407 2468
Fragments arranged by size:
2468 2407 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI