Restriction map for
pFN21AE2233
(Circular) (Six-base) MAPSORT of: pFN21AE2233.seq Check: 1998 from: 1 to: 6691
pFN21AE2233 6691 bp
With 182 enzymes: SgfI *
August 16, 2010 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4501 278
Size: 53 83 186 936 2965 2468
Fragments arranged by size:
2965 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4034 1937
Size: 2097 4594
Acc65I G'GTAC_C
Cuts at: 4012 4012
Size: 6691
AclI AA'CG_TT
Cuts at: 1563 4818 5191 1563
Size: 3255 373 3063
Fragments arranged by size:
3255 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4754 6138 6406 1632
Size: 3122 1384 268 1917
Fragments arranged by size:
3122 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2276 820
Size: 197 1259 5235
Fragments arranged by size:
5235 1259 197
AflIII A'CryG_T
Cuts at: 1203 3567 5590 6567 1203
Size: 2364 2023 977 1327
Fragments arranged by size:
2364 2023 1327 977
AgeI A'CCGG_T
Cuts at: 5522 5522
Size: 6691
AhdI GACnn_n'nnGTC
Cuts at: 5420 5420
Size: 6691
AleI CACnn'nnGTG
Cuts at: 1166 3072 1166
Size: 1906 4785
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6691
AlwNI CAG_nnn'CTG
Cuts at: 2933 4434 6006 2933
Size: 1501 1572 3618
Fragments arranged by size:
3618 1572 1501
ApaI G_GGCC'C
Cuts at: 2022 2066 2022
Size: 44 6647
ApaLI G'TGCA_C
Cuts at: 4748 5904 4748
Size: 1156 5535
ApoI r'AATT_y
Cuts at: 1493 2462 3551 3682 4000 4226 4378 6487 1493
Size: 969 1089 131 318 226 152 2109 1697
Fragments arranged by size:
2109 1697 1089 969 318 226 152 131
AseI AT'TA_AT
Cuts at: 160 1032 5245 160
Size: 872 4213 1606
Fragments arranged by size:
4213 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6691
AvaI C'yCGr_G
Cuts at: 1870 1943 2059 4016 1870
Size: 73 116 1957 4545
Fragments arranged by size:
4545 1957 116 73
BamHI G'GATC_C
Cuts at: 3010 3721 3839 4021 4388 3010
Size: 711 118 182 367 5313
Fragments arranged by size:
5313 711 367 182 118
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4012 5467 618
Size: 325 217 627 2225 1455 1842
Fragments arranged by size:
2225 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2022 2066 2546 4010 1391
Size: 631 44 480 1464 4072
Fragments arranged by size:
4072 1464 631 480 44
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2120 928
Size: 971 221 5499
Fragments arranged by size:
5499 971 221
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4882 4916 1903
Size: 34 2945 34 3678
Fragments arranged by size:
3678 2945 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4583 5793 4583
Size: 1210 5481
BclI T'GATC_A
Cuts at: 1548 3961 1548
Size: 2413 4278
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5302 136
Size: 107 122 71 631 605 3630 1525
Fragments arranged by size:
3630 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2327 6686 2327
Size: 4359 2332
BlpI GC'TnA_GC
Cuts at: 2553 2638 4108 2553
Size: 85 1470 5136
Fragments arranged by size:
5136 1470 85
Bme1580I G_kGCm'C
Cuts at: 1790 2022 2066 2740 4752 5908 1790
Size: 232 44 674 2012 1156 2573
Fragments arranged by size:
2573 2012 1156 674 232 44
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6691
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4422 5380 442
Size: 416 53 83 399 24 3005 958 1753
Fragments arranged by size:
3005 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3908 1056
Size: 2852 3839
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2134 2578 2134
Size: 444 6247
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2213 2910 5351 1239
Size: 974 697 2441 2579
Fragments arranged by size:
2579 2441 974 697
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2334 4816 5681 5979 2334
Size: 2482 865 298 3046
Fragments arranged by size:
3046 2482 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2348 5354 882
Size: 617 849 3006 2219
Fragments arranged by size:
3006 2219 849 617
BsaAI yAC'GTr
Cuts at: 493 1204 4482 493
Size: 711 3278 2702
Fragments arranged by size:
3278 2702 711
BsaBI GATnn'nnATC
Cuts at: 6685 6685
Size: 6691
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4498 4880 5515 275
Size: 53 83 186 936 2965 382 635 1451
Fragments arranged by size:
2965 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5124 5522 5796 5943 6354 5124
Size: 398 274 147 411 5461
Fragments arranged by size:
5461 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2231 2261 1178
Size: 30 165 30 828 30 5608
Fragments arranged by size:
5608 828 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3558 1186
Size: 2372 4319
BseYI C'CCAG_C
Cuts at: 5894 6390 5894
Size: 496 6195
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6118
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4141 4902 5930 1540
Size: 275 186 2140 761 1028 2301
Fragments arranged by size:
2301 2140 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2518 2546 3069 4752 4837 5908 2518
Size: 28 523 1683 85 1071 3301
Fragments arranged by size:
3301 1683 1071 523 85 28
BsmI GAATG_Cn'
Cuts at: 4202 4295 4202
Size: 93 6598
BsmBI CGTCTCn'nnnn_
Cuts at: 3645 3645
Size: 6691
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2022 2066 2518 2546 2740 3069 4010
Size: 399 232 44 452 28 194 329 941
Cuts at: 4010 4752 4837 5908 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 941 742 452 399 329 232
194 85 44 28
BspEI T'CCGG_A
Cuts at: 6354 6354
Size: 6691
BspHI T'CATG_A
Cuts at: 4580 6310 4580
Size: 1730 4961
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4046 844
Size: 3202 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2306 4578 1394
Size: 429 483 2272 3507
Fragments arranged by size:
3507 2272 483 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2967 3060 5186 5360 65
Size: 1183 1719 93 2126 174 1396
Fragments arranged by size:
2126 1719 1396 1183 174 93
BsrFI r'CCGG_y
Cuts at: 1700 1810 5335 5522 1700
Size: 110 3525 187 2869
Fragments arranged by size:
3525 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6691
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6691
BssSI C'ACGA_G
Cuts at: 4751 5763 4751
Size: 1012 5679
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2225 794
Size: 1431 5260
BstBI TT'CG_AA
Cuts at: 5495 5495
Size: 6691
BstEII G'GTnAC_C
Cuts at: 1184 2315 1184
Size: 1131 5560
BstXI CCAn_nnnn'nTGG
Cuts at: 3284 3284
Size: 6691
BstYI r'GATC_y
Cuts at: 1972 2327 3010 3721 3839 4021 4388 4773 4790
Size: 355 683 711 118 182 367 385 17
Cuts at: 4790 6242 6328 6340 6686 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 711 683 385 367 355 346
182 118 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2787 3462 513
Size: 552 1722 675 3742
Fragments arranged by size:
3742 1722 675 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5952
Fragments arranged by size:
5952 683 56
BtsI GCAGTG_nn'
Cuts at: 2904 3216 4207 5001 5021 2904
Size: 312 991 794 20 4574
Fragments arranged by size:
4574 991 794 312 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6691
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6656
DraI TTT'AAA
Cuts at: 3995 3995
Size: 6691
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2405 5698 809
Size: 507 1089 3293 1802
Fragments arranged by size:
3293 1802 1089 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2760 4138 5027
Size: 54 1425 50 275 18 930 1378 889
Cuts at: 5027 6656 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1378 930 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4138 1537
Size: 275 2326 4090
Fragments arranged by size:
4090 2326 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3161 3766 3894 4621 1353
Size: 1808 605 128 727 3423
Fragments arranged by size:
3423 1808 727 605 128
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2101 5278 5652 5798 387
Size: 1064 138 512 3177 374 146 1280
Fragments arranged by size:
3177 1280 1064 512 374 146 138
EcoICRI GAG'CTC
Cuts at: 2544 2544
Size: 6691
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2213 2910 4754 5351 6138 6406 1239
Size: 393 581 697 1844 597 787 268 1524
Fragments arranged by size:
1844 1524 787 697 597 581 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2019 2063 2019
Size: 44 6647
EcoRI G'AATT_C
Cuts at: 4000 4378 4000
Size: 378 6313
FspI TGC'GCA
Cuts at: 5197 5197
Size: 6691
HaeII r_GCGC'y
Cuts at: 1678 5838 1678
Size: 4160 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2114 2146 2345 2377 2621
Size: 32 135 32 542 32 199 32 244
Cuts at: 2621 2653 3713 3745 5332 5364 5406 5438 1373
Size: 32 1060 32 1587 32 42 32 2626
Fragments arranged by size:
2626 1587 1060 542 244 199 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4035 4281 669
Size: 988 281 2097 246 3079
Fragments arranged by size:
3079 2097 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2139 748
Size: 1391 5300
HpaI GTT'AAC
Cuts at: 4281 4281
Size: 6691
KpnI G_GTAC'C
Cuts at: 4016 4016
Size: 6691
MfeI C'AATT_G
Cuts at: 4290 4290
Size: 6691
MluI A'CGCG_T
Cuts at: 6567 6567
Size: 6691
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5805 5989 1305
Size: 4500 184 2007
Fragments arranged by size:
4500 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2762 10
Size: 54 1425 343 930 3939
Fragments arranged by size:
3939 1425 930 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2313 3072 4649 5008 5167 518
Size: 648 1147 759 1577 359 159 2042
Fragments arranged by size:
2042 1577 1147 759 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4107 4784 5932 6177 1606
Size: 2501 677 1148 245 2120
Fragments arranged by size:
2501 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2787 3462 513
Size: 552 1722 675 3742
Fragments arranged by size:
3742 1722 675 552
NdeI CA'TA_TG
Cuts at: 387 3748 387
Size: 3361 3330
NheI G'CTAG_C
Cuts at: 1052 3904 1052
Size: 2852 3839
NotI GC'GGCC_GC
Cuts at: 4138 4138
Size: 6691
NruI TCG'CGA
Cuts at: 2506 2506
Size: 6691
NspI r_CATG'y
Cuts at: 1132 1229 4049 5594 1132
Size: 97 2820 1545 2229
Fragments arranged by size:
2820 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3617 1348
Size: 2269 4422
PciI A'CATG_T
Cuts at: 5590 5590
Size: 6691
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6691
PmeI GTTT'AAAC
Cuts at: 3995 3995
Size: 6691
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4760 4792 6273 6305 4760
Size: 32 1481 32 5146
Fragments arranged by size:
5146 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6691
PsiI TTA'TAA
Cuts at: 3510 4261 3510
Size: 751 5940
PspOMI G'GGCC_C
Cuts at: 2018 2062 2018
Size: 44 6647
PstI C_TGCA'G
Cuts at: 830 2203 3404 4043 830
Size: 1373 1201 639 3478
Fragments arranged by size:
3478 1373 1201 639
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6691
SacI G_AGCT'C
Cuts at: 2546 2546
Size: 6691
SalI G'TCGA_C
Cuts at: 1936 4033 1936
Size: 2097 4594
SapI GCTCTTCn'nnn_
Cuts at: 3894 3894
Size: 6691
SbfI CC_TGCA'GG
Cuts at: 4043 4043
Size: 6691
ScaI AGT'ACT
Cuts at: 3329 4939 6651 3329
Size: 1610 1712 3369
Fragments arranged by size:
3369 1712 1610
SfcI C'TryA_G
Cuts at: 826 1045 2199 2586 3400 4039 5174 5855 6046
Size: 219 1154 387 814 639 1135 681 191
Cuts at: 6046 826
Size: 1471
Fragments arranged by size:
1471 1154 1135 814 681 639 387 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6691
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6691
SmaI CCC'GGG
Cuts at: 1872 2061 4018 1872
Size: 189 1957 4545
Fragments arranged by size:
4545 1957 189
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2276 2349 4795 5696 5958 820
Size: 197 926 333 73 2446 901 262 1553
Fragments arranged by size:
2446 1553 926 901 333 262 197 73
SnaBI TAC'GTA
Cuts at: 493 4482 493
Size: 3989 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6691
SphI G_CATG'C
Cuts at: 1132 4049 1132
Size: 2917 3774
SspI AAT'ATT
Cuts at: 5 52 3515 4615 5
Size: 47 3463 1100 2081
Fragments arranged by size:
3463 2081 1100 47
StuI AGG'CCT
Cuts at: 1517 3452 1517
Size: 1935 4756
StyI C'CwwG_G
Cuts at: 513 1065 2787 3462 4130 513
Size: 552 1722 675 668 3074
Fragments arranged by size:
3074 1722 675 668 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3630 3859 4718 4735 4888 5073 5518
Size: 1255 2050 229 859 17 153 185 445
Cuts at: 5518 325
Size: 1498
Fragments arranged by size:
2050 1498 1255 859 445 229 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3189 3327 4937
Size: 274 80 33 51 1443 1212 138 1610
Cuts at: 4937 6649 96
Size: 1712 138
Fragments arranged by size:
1712 1610 1443 1212 274 138 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2035 2067 2768 2800 6651 6683 2035
Size: 32 701 32 3851 32 2043
Fragments arranged by size:
3851 2043 701 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2208 1316
Size: 892 5799
XbaI T'CTAG_A
Cuts at: 4027 4027
Size: 6691
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6691
XmaI C'CCGG_G
Cuts at: 1870 2059 4016 1870
Size: 189 1957 4545
Fragments arranged by size:
4545 1957 189
XmnI GAAnn'nnTTC
Cuts at: 2484 4820 2484
Size: 2336 4355
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4499 276
Size: 53 83 186 936 2965 2468
Fragments arranged by size:
2965 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NruI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NsiI
PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII
SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI