Restriction map for
pFN21AE2230
(Circular) (Six-base) MAPSORT of: pFN21AE2230.seq Check: 7290 from: 1 to: 6112
pFN21AE2230 6112 bp
With 182 enzymes: SgfI *
July 6, 2012 17:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3922 278
Size: 53 83 186 936 2386 2468
Fragments arranged by size:
2468 2386 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2454 3455 1937
Size: 517 1001 4594
Fragments arranged by size:
4594 1001 517
Acc65I G'GTAC_C
Cuts at: 3433 3433
Size: 6112
AclI AA'CG_TT
Cuts at: 1563 4239 4612 1563
Size: 2676 373 3063
Fragments arranged by size:
3063 2676 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2213 2542 3110 3170 3176 4175 5559 5827
Size: 581 329 568 60 6 999 1384 268
Cuts at: 5827 1632
Size: 1917
Fragments arranged by size:
1917 1384 999 581 568 329 268 60
6
AflII C'TTAA_G
Cuts at: 820 1017 2054 820
Size: 197 1037 4878
Fragments arranged by size:
4878 1037 197
AflIII A'CryG_T
Cuts at: 1203 2200 3342 5011 5988 1203
Size: 997 1142 1669 977 1327
Fragments arranged by size:
1669 1327 1142 997 977
AgeI A'CCGG_T
Cuts at: 4943 4943
Size: 6112
AhdI GACnn_n'nnGTC
Cuts at: 4841 4841
Size: 6112
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6112
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6112
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2238 2270 2238
Size: 32 6080
AlwNI CAG_nnn'CTG
Cuts at: 2738 3088 3855 5427 2738
Size: 350 767 1572 3423
Fragments arranged by size:
3423 1572 767 350
ApaI G_GGCC'C
Cuts at: 3166 3166
Size: 6112
ApaLI G'TGCA_C
Cuts at: 3121 4169 5325 3121
Size: 1048 1156 3908
Fragments arranged by size:
3908 1156 1048
ApoI r'AATT_y
Cuts at: 1493 2495 2662 2857 2961 3173 3389 3421 3647
Size: 1002 167 195 104 212 216 32 226
Cuts at: 3647 3799 5908 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1002 226 216 212 195 167
152 104 32
AseI AT'TA_AT
Cuts at: 160 1032 4666 160
Size: 872 3634 1606
Fragments arranged by size:
3634 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6112
AvaI C'yCGr_G
Cuts at: 1870 1943 3437 1870
Size: 73 1494 4545
Fragments arranged by size:
4545 1494 73
BamHI G'GATC_C
Cuts at: 3442 3809 3442
Size: 367 5745
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3433 4888 618
Size: 325 217 627 1646 1455 1842
Fragments arranged by size:
1842 1646 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2359 2389 3166 3243 3431 1391
Size: 968 30 777 77 188 4072
Fragments arranged by size:
4072 968 777 188 77 30
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3345 928
Size: 971 1446 3695
Fragments arranged by size:
3695 1446 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4303 4337 1903
Size: 34 2366 34 3678
Fragments arranged by size:
3678 2366 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4004 5214 4004
Size: 1210 4902
BclI T'GATC_A
Cuts at: 1548 2776 1548
Size: 1228 4884
BfrBI ATG'CAT
Cuts at: 2773 2773
Size: 6112
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4723 136
Size: 107 122 71 631 605 3051 1525
Fragments arranged by size:
3051 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6107 6107
Size: 6112
BlpI GC'TnA_GC
Cuts at: 3529 3529
Size: 6112
Bme1580I G_kGCm'C
Cuts at: 1790 3125 3166 4173 5329 1790
Size: 1335 41 1007 1156 2573
Fragments arranged by size:
2573 1335 1156 1007 41
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6112
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2353 3843 4801
Size: 416 53 83 399 24 936 1490 958
Cuts at: 4801 442
Size: 1753
Fragments arranged by size:
1753 1490 958 936 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6112
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3257 3257
Size: 6112
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2457 2727 2760 4772 1239
Size: 1218 270 33 2012 2579
Fragments arranged by size:
2579 2012 1218 270 33
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4237 5102 5400 4237
Size: 865 298 4949
Fragments arranged by size:
4949 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2026 2992 4775 882
Size: 617 527 966 1783 2219
Fragments arranged by size:
2219 1783 966 617 527
BsaAI yAC'GTr
Cuts at: 493 1204 3120 3903 493
Size: 711 1916 783 2702
Fragments arranged by size:
2702 1916 783 711
BsaBI GATnn'nnATC
Cuts at: 6106 6106
Size: 6112
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3919 4301 4936 275
Size: 53 83 186 936 2386 382 635 1451
Fragments arranged by size:
2386 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3298 4545 4943 5217 5364 5775 3298
Size: 1247 398 274 147 411 3635
Fragments arranged by size:
3635 1247 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2376 2406 1178
Size: 30 165 30 973 30 4884
Fragments arranged by size:
4884 973 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2397 3231 1186
Size: 1211 834 4067
Fragments arranged by size:
4067 1211 834
BseYI C'CCAG_C
Cuts at: 3236 5315 5811 3236
Size: 2079 496 3537
Fragments arranged by size:
3537 2079 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2751 1163
Size: 573 1015 4524
Fragments arranged by size:
4524 1015 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3562 4323 5351 1540
Size: 275 186 1561 761 1028 2301
Fragments arranged by size:
2301 1561 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3125 4173 4258 5329 3125
Size: 1048 85 1071 3908
Fragments arranged by size:
3908 1071 1048 85
BsmI GAATG_Cn'
Cuts at: 2842 3623 3716 2842
Size: 781 93 5238
Fragments arranged by size:
5238 781 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2359 2389 3125 3166 3243 3431 4173
Size: 399 569 30 736 41 77 188 742
Cuts at: 4173 4258 5329 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 736 569 399 188 85
77 41 30
BspEI T'CCGG_A
Cuts at: 5775 5775
Size: 6112
BspHI T'CATG_A
Cuts at: 4001 5731 4001
Size: 1730 4382
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3467 844
Size: 2623 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3999 1394
Size: 429 2176 3507
Fragments arranged by size:
3507 2176 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2374 2757 4607 4781 65
Size: 1183 1126 383 1850 174 1396
Fragments arranged by size:
1850 1396 1183 1126 383 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4756 4943 1700
Size: 110 2946 187 2869
Fragments arranged by size:
2946 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6112
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6112
BssSI C'ACGA_G
Cuts at: 4172 5184 4172
Size: 1012 5100
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6112
BstBI TT'CG_AA
Cuts at: 4916 4916
Size: 6112
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6112
BstYI r'GATC_y
Cuts at: 1972 3442 3809 4194 4211 5663 5749 5761 6107
Size: 1470 367 385 17 1452 86 12 346
Cuts at: 6107 1972
Size: 1977
Fragments arranged by size:
1977 1470 1452 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2455 2455
Size: 6112
Bsu36I CC'TnA_GG
Cuts at: 2037 2037
Size: 6112
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5560
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5373
Fragments arranged by size:
5373 683 56
BtsI GCAGTG_nn'
Cuts at: 3069 3628 4422 4442 3069
Size: 559 794 20 4739
Fragments arranged by size:
4739 794 559 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6112
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6077
DraI TTT'AAA
Cuts at: 3416 3416
Size: 6112
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5119 809
Size: 507 3803 1802
Fragments arranged by size:
3803 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3135 3559 4448
Size: 54 1425 50 275 18 1305 424 889
Cuts at: 4448 6077 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1305 889 424 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3559 1537
Size: 275 1747 4090
Fragments arranged by size:
4090 1747 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3071 3328 4042 1353
Size: 1718 257 714 3423
Fragments arranged by size:
3423 1718 714 257
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4699 5073 5219 387
Size: 1064 138 3110 374 146 1280
Fragments arranged by size:
3110 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2213 2457 2542 2727 2760 3110 3170
Size: 393 581 244 85 185 33 350 60
Cuts at: 3170 3176 4175 4772 5559 5827 1239
Size: 6 999 597 787 268 1524
Fragments arranged by size:
1524 999 787 597 581 393 350 268
244 185 85 60 33 6
EcoO109I rG'GnC_Cy
Cuts at: 3163 3163
Size: 6112
EcoRI G'AATT_C
Cuts at: 2857 2961 3421 3799 2857
Size: 104 460 378 5170
Fragments arranged by size:
5170 460 378 104
EcoRV GAT'ATC
Cuts at: 2025 2025
Size: 6112
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2080 2534 2080
Size: 454 5658
FspI TGC'GCA
Cuts at: 4618 4618
Size: 6112
HaeII r_GCGC'y
Cuts at: 1678 5259 1678
Size: 3581 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2376 2408 3299 3331 4753
Size: 32 135 32 804 32 891 32 1422
Cuts at: 4753 4785 4827 4859 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1422 891 804 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3456 3702 669
Size: 988 281 1518 246 3079
Fragments arranged by size:
3079 1518 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6112
HpaI GTT'AAC
Cuts at: 3702 3702
Size: 6112
KpnI G_GTAC'C
Cuts at: 3437 3437
Size: 6112
MfeI C'AATT_G
Cuts at: 3711 3711
Size: 6112
MluI A'CGCG_T
Cuts at: 5988 5988
Size: 6112
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2332 5226 5410 1305
Size: 1027 2894 184 2007
Fragments arranged by size:
2894 2007 1027 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4290
Fragments arranged by size:
4290 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2394 2594 3308 4070 4429 4588 518
Size: 648 1228 200 714 762 359 159 2042
Fragments arranged by size:
2042 1228 762 714 648 359 200 159
MspA1I CmG'CkG
Cuts at: 1606 3077 3085 3265 3528 4205 5353 5598 1606
Size: 1471 8 180 263 677 1148 245 2120
Fragments arranged by size:
2120 1471 1148 677 263 245 180 8
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5560
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6112
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6112
NotI GC'GGCC_GC
Cuts at: 3559 3559
Size: 6112
NsiI A_TGCA'T
Cuts at: 2775 2775
Size: 6112
NspI r_CATG'y
Cuts at: 1132 1229 2204 3346 3470 5015 1132
Size: 97 975 1142 124 1545 2229
Fragments arranged by size:
2229 1545 1142 975 124 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6112
PciI A'CATG_T
Cuts at: 2200 3342 5011 2200
Size: 1142 1669 3301
Fragments arranged by size:
3301 1669 1142
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6112
PmeI GTTT'AAAC
Cuts at: 3416 3416
Size: 6112
PmlI CAC'GTG
Cuts at: 3120 3120
Size: 6112
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4181 4213 5694 5726 4181
Size: 32 1481 32 4567
Fragments arranged by size:
4567 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6112
PsiI TTA'TAA
Cuts at: 3682 3682
Size: 6112
PspOMI G'GGCC_C
Cuts at: 3162 3162
Size: 6112
PstI C_TGCA'G
Cuts at: 830 3076 3270 3464 830
Size: 2246 194 194 3478
Fragments arranged by size:
3478 2246 194 194
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6112
PvuII CAG'CTG
Cuts at: 3085 3265 3085
Size: 180 5932
SalI G'TCGA_C
Cuts at: 1936 3454 1936
Size: 1518 4594
SbfI CC_TGCA'GG
Cuts at: 3464 3464
Size: 6112
ScaI AGT'ACT
Cuts at: 4360 6072 4360
Size: 1712 4400
SfcI C'TryA_G
Cuts at: 826 1045 2980 3072 3266 3369 3460 4595 5276
Size: 219 1935 92 194 103 91 1135 681
Cuts at: 5276 5467 826
Size: 191 1471
Fragments arranged by size:
1935 1471 1135 681 219 194 191 103
92 91
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6112
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6112
SmaI CCC'GGG
Cuts at: 1872 3439 1872
Size: 1567 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2054 4216 5117 5379 820
Size: 197 926 111 2162 901 262 1553
Fragments arranged by size:
2162 1553 926 901 262 197 111
SnaBI TAC'GTA
Cuts at: 493 3903 493
Size: 3410 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6112
SphI G_CATG'C
Cuts at: 1132 3470 1132
Size: 2338 3774
SspI AAT'ATT
Cuts at: 5 52 2323 4036 5
Size: 47 2271 1713 2081
Fragments arranged by size:
2271 2081 1713 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6112
StyI C'CwwG_G
Cuts at: 513 1065 3551 513
Size: 552 2486 3074
Fragments arranged by size:
3074 2486 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2973 4139 4156 4309 4494 4939 325
Size: 1255 1393 1166 17 153 185 445 1498
Fragments arranged by size:
1498 1393 1255 1166 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2194 2635 2689
Size: 274 80 33 51 1443 217 441 54
Cuts at: 2689 3014 4358 6070 96
Size: 325 1344 1712 138
Fragments arranged by size:
1712 1443 1344 441 325 274 217 138
80 54 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6072 6104 6072
Size: 32 6080
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6112
XbaI T'CTAG_A
Cuts at: 2719 3448 2719
Size: 729 5383
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6112
XmaI C'CCGG_G
Cuts at: 1870 3437 1870
Size: 1567 4545
XmnI GAAnn'nnTTC
Cuts at: 4241 4241
Size: 6112
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3920 276
Size: 53 83 186 936 2386 2468
Fragments arranged by size:
2468 2386 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI Bpu10I
BsiWI BsmBI BstXI DraIII EcoICRI EcoNI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PfoI PpuMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI