Restriction map for
pFN21AE2227
(Circular) (Six-base) MAPSORT of: pFN21AE2227.seq Check: 6034 from: 1 to: 5872
pFN21AE2227 5872 bp
With 182 enzymes: SgfI *
August 16, 2010 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3682 278
Size: 53 83 186 936 2146 2468
Fragments arranged by size:
2468 2146 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3215 1937
Size: 1278 4594
Acc65I G'GTAC_C
Cuts at: 2744 3063 3193 2744
Size: 319 130 5423
Fragments arranged by size:
5423 319 130
AclI AA'CG_TT
Cuts at: 1563 3999 4372 1563
Size: 2436 373 3063
Fragments arranged by size:
3063 2436 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2760 3935 5319 5587 1632
Size: 1128 1175 1384 268 1917
Fragments arranged by size:
1917 1384 1175 1128 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5675
AflIII A'CryG_T
Cuts at: 1203 4771 5748 1203
Size: 3568 977 1327
Fragments arranged by size:
3568 1327 977
AgeI A'CCGG_T
Cuts at: 4703 4703
Size: 5872
AhdI GACnn_n'nnGTC
Cuts at: 4601 4601
Size: 5872
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5872
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5872
AlwNI CAG_nnn'CTG
Cuts at: 2477 2513 3032 3615 5187 2477
Size: 36 519 583 1572 3162
Fragments arranged by size:
3162 1572 583 519 36
ApaLI G'TGCA_C
Cuts at: 3929 5085 3929
Size: 1156 4716
ApoI r'AATT_y
Cuts at: 1493 2414 2668 3073 3181 3407 3559 5668 1493
Size: 921 254 405 108 226 152 2109 1697
Fragments arranged by size:
2109 1697 921 405 254 226 152 108
AseI AT'TA_AT
Cuts at: 160 1032 4426 160
Size: 872 3394 1606
Fragments arranged by size:
3394 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5872
AvaI C'yCGr_G
Cuts at: 1870 1943 2455 3197 1870
Size: 73 512 742 4545
Fragments arranged by size:
4545 742 512 73
BamHI G'GATC_C
Cuts at: 3202 3569 3202
Size: 367 5505
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2051 2639 2744 3063 3193
Size: 325 217 627 264 588 105 319 130
Cuts at: 3193 4648 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 588 325 319 264 217
130 105
BanII G_rGCy'C
Cuts at: 1391 3191 1391
Size: 1800 4072
BbeI G_GCGC'C
Cuts at: 2055 2055
Size: 5872
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4901
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4063 4097 1903
Size: 34 2126 34 3678
Fragments arranged by size:
3678 2126 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3764 4974 3764
Size: 1210 4662
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5872
BfrBI ATG'CAT
Cuts at: 3165 3165
Size: 5872
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2146 2186 4483
Size: 107 122 71 631 605 474 40 2297
Cuts at: 4483 136
Size: 1525
Fragments arranged by size:
2297 1525 631 605 474 122 107 71
40
BglII A'GATC_T
Cuts at: 5867 5867
Size: 5872
BlpI GC'TnA_GC
Cuts at: 3289 3289
Size: 5872
Bme1580I G_kGCm'C
Cuts at: 1790 3933 5089 1790
Size: 2143 1156 2573
Fragments arranged by size:
2573 2143 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5872
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2071 2439 2645
Size: 416 53 83 399 24 654 368 206
Cuts at: 2645 3603 4561 442
Size: 958 958 1753
Fragments arranged by size:
1753 958 958 654 416 399 368 206
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5872
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2107 2267 2370 2535 4532 1239
Size: 868 160 103 165 1997 2579
Fragments arranged by size:
2579 1997 868 165 160 103
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2996 3997 4862 5160 2996
Size: 1001 865 298 3708
Fragments arranged by size:
3708 1001 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4535 882
Size: 617 3036 2219
Fragments arranged by size:
3036 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3663 493
Size: 711 2459 2702
Fragments arranged by size:
2702 2459 711
BsaBI GATnn'nnATC
Cuts at: 5866 5866
Size: 5872
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2052 3679 4061 4696
Size: 53 83 186 936 519 1627 382 635
Cuts at: 4696 275
Size: 1451
Fragments arranged by size:
1627 1451 936 635 519 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4305 4703 4977 5124 5535 4305
Size: 398 274 147 411 4642
Fragments arranged by size:
4642 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2056 2086 2493 2523 1178
Size: 30 165 30 653 30 407 30 4527
Fragments arranged by size:
4527 653 407 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5872
BseYI C'CCAG_C
Cuts at: 2103 2470 5075 5571 2103
Size: 367 2605 496 2404
Fragments arranged by size:
2605 2404 496 367
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2186 2327 1163
Size: 573 450 141 4708
Fragments arranged by size:
4708 573 450 141
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3322 4083 5111 1540
Size: 275 186 1321 761 1028 2301
Fragments arranged by size:
2301 1321 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2089 2157 2603 3933 4018 5089 2089
Size: 68 446 1330 85 1071 2872
Fragments arranged by size:
2872 1330 1071 446 85 68
BsmI GAATG_Cn'
Cuts at: 3383 3476 3383
Size: 93 5779
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2089 2157 2603 3191 3933 4018 5089
Size: 399 299 68 446 588 742 85 1071
Cuts at: 5089 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 588 446 399 299 85
68
BspEI T'CCGG_A
Cuts at: 5535 5535
Size: 5872
BspHI T'CATG_A
Cuts at: 3761 5491 3761
Size: 1730 4142
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3227 844
Size: 2383 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3759 1394
Size: 429 1936 3507
Fragments arranged by size:
3507 1936 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2695 4367 4541 65
Size: 1183 1447 1672 174 1396
Fragments arranged by size:
1672 1447 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4516 4703 1700
Size: 110 2706 187 2869
Fragments arranged by size:
2869 2706 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5872
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5872
BssSI C'ACGA_G
Cuts at: 3932 4944 3932
Size: 1012 4860
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5872
BstBI TT'CG_AA
Cuts at: 4676 4676
Size: 5872
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5872
BstYI r'GATC_y
Cuts at: 1972 2345 3202 3569 3954 3971 5423 5509 5521
Size: 373 857 367 385 17 1452 86 12
Cuts at: 5521 5867 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 857 385 373 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5320
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5133
Fragments arranged by size:
5133 683 56
BtsI GCAGTG_nn'
Cuts at: 2601 2628 3388 4182 4202 2601
Size: 27 760 794 20 4271
Fragments arranged by size:
4271 794 760 27 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5872
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5837
DraI TTT'AAA
Cuts at: 3176 3176
Size: 5872
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2126 4879 809
Size: 507 810 2753 1802
Fragments arranged by size:
2753 1802 810 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2333 2383 3319
Size: 54 1425 50 275 18 503 50 936
Cuts at: 3319 4208 5837 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 936 889 503 275 54 50
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3319 1537
Size: 275 1507 4090
Fragments arranged by size:
4090 1507 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2162 2223 3802 1353
Size: 809 61 1579 3423
Fragments arranged by size:
3423 1579 809 61
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4459 4833 4979 387
Size: 1064 138 2870 374 146 1280
Fragments arranged by size:
2870 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2107 2267 2370 2535 2760 3935 4532
Size: 393 475 160 103 165 225 1175 597
Cuts at: 4532 5319 5587 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1175 787 597 475 393 268 225
165 160 103
EcoO109I rG'GnC_Cy
Cuts at: 2033 2033
Size: 5872
EcoRI G'AATT_C
Cuts at: 3181 3559 3181
Size: 378 5494
FspI TGC'GCA
Cuts at: 4378 4378
Size: 5872
HaeII r_GCGC'y
Cuts at: 1678 2026 2055 2192 2201 5019 1678
Size: 348 29 137 9 2818 2531
Fragments arranged by size:
2818 2531 348 137 29 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2056 2088 2330 2362 4513
Size: 32 135 32 484 32 242 32 2151
Cuts at: 4513 4545 4587 4619 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2151 484 242 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2878 3216 3462 669
Size: 988 281 940 338 246 3079
Fragments arranged by size:
3079 988 940 338 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5872
HpaI GTT'AAC
Cuts at: 2878 3462 2878
Size: 584 5288
KasI G'GCGC_C
Cuts at: 2051 2051
Size: 5872
KpnI G_GTAC'C
Cuts at: 2748 3067 3197 2748
Size: 319 130 5423
Fragments arranged by size:
5423 319 130
MfeI C'AATT_G
Cuts at: 3471 3471
Size: 5872
MluI A'CGCG_T
Cuts at: 5748 5748
Size: 5872
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2344 2966 3146 4986 5170 1305
Size: 1039 622 180 1840 184 2007
Fragments arranged by size:
2007 1840 1039 622 184 180
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4050
Fragments arranged by size:
4050 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3830 4189 4348 518
Size: 648 843 1821 359 159 2042
Fragments arranged by size:
2042 1821 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2041 3288 3965 5113 5358 1606
Size: 435 1247 677 1148 245 2120
Fragments arranged by size:
2120 1247 1148 677 435 245
NarI GG'CG_CC
Cuts at: 2052 2052
Size: 5872
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5320
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5872
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5872
NotI GC'GGCC_GC
Cuts at: 3319 3319
Size: 5872
NsiI A_TGCA'T
Cuts at: 3167 3167
Size: 5872
NspI r_CATG'y
Cuts at: 1132 1229 2650 3230 4775 1132
Size: 97 1421 580 1545 2229
Fragments arranged by size:
2229 1545 1421 580 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5872
PciI A'CATG_T
Cuts at: 4771 4771
Size: 5872
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5872
PfoI T'CCnGG_A
Cuts at: 2926 2926
Size: 5872
PmeI GTTT'AAAC
Cuts at: 3176 3176
Size: 5872
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3941 3973 5454 5486 3941
Size: 32 1481 32 4327
Fragments arranged by size:
4327 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2033 2033
Size: 5872
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5872
PsiI TTA'TAA
Cuts at: 3442 3442
Size: 5872
PstI C_TGCA'G
Cuts at: 830 3224 830
Size: 2394 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5872
SalI G'TCGA_C
Cuts at: 1936 3214 1936
Size: 1278 4594
SapI GCTCTTCn'nnn_
Cuts at: 2162 2162
Size: 5872
SbfI CC_TGCA'GG
Cuts at: 3224 3224
Size: 5872
ScaI AGT'ACT
Cuts at: 4120 5832 4120
Size: 1712 4160
SfcI C'TryA_G
Cuts at: 826 1045 3220 4355 5036 5227 826
Size: 219 2175 1135 681 191 1471
Fragments arranged by size:
2175 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2053 2053
Size: 5872
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5872
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5872
SmaI CCC'GGG
Cuts at: 1872 3199 1872
Size: 1327 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3011 3976 4877 5139 820
Size: 197 926 1068 965 901 262 1553
Fragments arranged by size:
1553 1068 965 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3663 493
Size: 3170 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5872
SphI G_CATG'C
Cuts at: 1132 3230 1132
Size: 2098 3774
SspI AAT'ATT
Cuts at: 5 52 2787 3796 5
Size: 47 2735 1009 2081
Fragments arranged by size:
2735 2081 1009 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5872
StyI C'CwwG_G
Cuts at: 513 1065 3311 513
Size: 552 2246 3074
Fragments arranged by size:
3074 2246 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2608 2627 3899 3916 4069 4254 4699
Size: 1255 1028 19 1272 17 153 185 445
Cuts at: 4699 325
Size: 1498
Fragments arranged by size:
1498 1272 1255 1028 445 185 153 19
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4118 5830 96
Size: 274 80 33 51 1443 2141 1712 138
Fragments arranged by size:
2141 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2274 2306 5832 5864 2274
Size: 32 3526 32 2282
Fragments arranged by size:
3526 2282 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5872
XbaI T'CTAG_A
Cuts at: 3208 3208
Size: 5872
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2857 2011
Size: 846 5026
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5872
XmaI C'CCGG_G
Cuts at: 1870 3197 1870
Size: 1327 4545
XmnI GAAnn'nnTTC
Cuts at: 2580 4001 2580
Size: 1421 4451
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3680 276
Size: 53 83 186 936 2146 2468
Fragments arranged by size:
2468 2146 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV
NruI PacI PmlI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SexAI SfiI SrfI SwaI