Restriction map for
pFN21AE2226
(Circular) (Six-base) MAPSORT of: pFN21AE2226.seq Check: 2334 from: 1 to: 5764
pFN21AE2226 5764 bp
With 182 enzymes: SgfI *
July 6, 2012 17:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3574 278
Size: 53 83 186 936 2038 2468
Fragments arranged by size:
2468 2038 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3107 1937
Size: 1170 4594
Acc65I G'GTAC_C
Cuts at: 3085 3085
Size: 5764
AclI AA'CG_TT
Cuts at: 1563 3891 4264 1563
Size: 2328 373 3063
Fragments arranged by size:
3063 2328 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3015 3827 5211 5479 1632
Size: 1383 812 1384 268 1917
Fragments arranged by size:
1917 1384 1383 812 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5567
AflIII A'CryG_T
Cuts at: 1203 2604 4663 5640 1203
Size: 1401 2059 977 1327
Fragments arranged by size:
2059 1401 1327 977
AgeI A'CCGG_T
Cuts at: 4595 4595
Size: 5764
AhdI GACnn_n'nnGTC
Cuts at: 2040 2175 4493 2040
Size: 135 2318 3311
Fragments arranged by size:
3311 2318 135
AleI CACnn'nnGTG
Cuts at: 1166 2355 1166
Size: 1189 4575
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5764
AlwNI CAG_nnn'CTG
Cuts at: 2323 3507 5079 2323
Size: 1184 1572 3008
Fragments arranged by size:
3008 1572 1184
ApaLI G'TGCA_C
Cuts at: 3821 4977 3821
Size: 1156 4608
ApoI r'AATT_y
Cuts at: 1493 2476 2895 3073 3299 3451 5560 1493
Size: 983 419 178 226 152 2109 1697
Fragments arranged by size:
2109 1697 983 419 226 178 152
AseI AT'TA_AT
Cuts at: 160 1032 4318 160
Size: 872 3286 1606
Fragments arranged by size:
3286 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5764
AvaI C'yCGr_G
Cuts at: 1870 1943 3089 1870
Size: 73 1146 4545
Fragments arranged by size:
4545 1146 73
BamHI G'GATC_C
Cuts at: 2460 2467 2698 3094 3461 2460
Size: 7 231 396 367 4763
Fragments arranged by size:
4763 396 367 231 7
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2134 2844 3085 4540 618
Size: 325 217 627 347 710 241 1455 1842
Fragments arranged by size:
1842 1455 710 627 347 325 241 217
BanII G_rGCy'C
Cuts at: 1391 2378 2420 2786 3003 3083 1391
Size: 987 42 366 217 80 4072
Fragments arranged by size:
4072 987 366 217 80 42
BbeI G_GCGC'C
Cuts at: 2848 2848
Size: 5764
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3021 928
Size: 971 1122 3671
Fragments arranged by size:
3671 1122 971
BbvCI CC'TCA_GC
Cuts at: 2983 2983
Size: 5764
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1998 2032 3955 3989 1903
Size: 34 61 34 1923 34 3678
Fragments arranged by size:
3678 1923 61 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3656 4866 3656
Size: 1210 4554
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5764
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2314 2411 4375
Size: 107 122 71 631 605 642 97 1964
Cuts at: 4375 136
Size: 1525
Fragments arranged by size:
1964 1525 642 631 605 122 107 97
71
BglII A'GATC_T
Cuts at: 5759 5759
Size: 5764
BlpI GC'TnA_GC
Cuts at: 2108 3002 3012 3181 2108
Size: 894 10 169 4691
Fragments arranged by size:
4691 894 169 10
Bme1580I G_kGCm'C
Cuts at: 1790 3825 4981 1790
Size: 2035 1156 2573
Fragments arranged by size:
2573 2035 1156
BmgBI CAC'GTC
Cuts at: 1318 2605 1318
Size: 1287 4477
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2698 3495 4453
Size: 416 53 83 399 24 1281 797 958
Cuts at: 4453 442
Size: 1753
Fragments arranged by size:
1753 1281 958 797 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5764
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2171 2210 2544 4424 1239
Size: 932 39 334 1880 2579
Fragments arranged by size:
2579 1880 932 334 39
Bpu10I CC'TnA_GC
Cuts at: 2785 2983 2785
Size: 198 5566
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2885 3889 4754 5052 2885
Size: 1004 865 298 3597
Fragments arranged by size:
3597 1004 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4427 882
Size: 617 2928 2219
Fragments arranged by size:
2928 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3555 493
Size: 711 2351 2702
Fragments arranged by size:
2702 2351 711
BsaBI GATnn'nnATC
Cuts at: 2720 5758 2720
Size: 3038 2726
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2845 3571 3953 4588
Size: 53 83 186 936 1312 726 382 635
Cuts at: 4588 275
Size: 1451
Fragments arranged by size:
1451 1312 936 726 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2271 2627 4197 4595 4869 5016 5427 2271
Size: 356 1570 398 274 147 411 2608
Fragments arranged by size:
2608 1570 411 398 356 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2144 2174 2594 2624 1178
Size: 30 165 30 741 30 420 30 4318
Fragments arranged by size:
4318 741 420 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2117 2816 1186
Size: 931 699 4134
Fragments arranged by size:
4134 931 699
BseYI C'CCAG_C
Cuts at: 2240 2433 2649 2770 4967 5463 2240
Size: 193 216 121 2197 496 2541
Fragments arranged by size:
2541 2197 496 216 193 121
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2825 1163
Size: 573 1089 4102
Fragments arranged by size:
4102 1089 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2022 3214 3975 5003 1540
Size: 275 186 21 1192 761 1028 2301
Fragments arranged by size:
2301 1192 1028 761 275 186 21
BsiHKAI G_wGCw'C
Cuts at: 3825 3910 4981 3825
Size: 85 1071 4608
Fragments arranged by size:
4608 1071 85
BsmI GAATG_Cn'
Cuts at: 3275 3368 3275
Size: 93 5671
BsmBI CGTCTCn'nnnn_
Cuts at: 2884 2884
Size: 5764
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2378 2420 2786 3003 3083 3825 3910
Size: 399 588 42 366 217 80 742 85
Cuts at: 3910 4981 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 588 399 366 217 85
80 42
BspEI T'CCGG_A
Cuts at: 2271 5427 2271
Size: 3156 2608
BspHI T'CATG_A
Cuts at: 2724 3653 5383 2724
Size: 929 1730 3105
Fragments arranged by size:
3105 1730 929
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3119 844
Size: 2275 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2089 2205 2554 3651 1394
Size: 429 266 116 349 1097 3507
Fragments arranged by size:
3507 1097 429 349 266 116
BsrDI GCAATG_nn'
Cuts at: 65 1248 2009 4259 4433 65
Size: 1183 761 2250 174 1396
Fragments arranged by size:
2250 1396 1183 761 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2669 4408 4595 1700
Size: 110 859 1739 187 2869
Fragments arranged by size:
2869 1739 859 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5764
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5764
BssSI C'ACGA_G
Cuts at: 2663 3824 4836 2663
Size: 1161 1012 3591
Fragments arranged by size:
3591 1161 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5764
BstBI TT'CG_AA
Cuts at: 4568 4568
Size: 5764
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5764
BstYI r'GATC_y
Cuts at: 1972 2460 2467 2698 2715 3094 3461 3846 3863
Size: 488 7 231 17 379 367 385 17
Cuts at: 3863 5315 5401 5413 5759 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 488 385 379 367 346 231
86 17 17 12 7
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4273
Fragments arranged by size:
4273 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5025
Fragments arranged by size:
5025 683 56
BtsI GCAGTG_nn'
Cuts at: 3280 4074 4094 3280
Size: 794 20 4950
Fragments arranged by size:
4950 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5764
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5729
DraI TTT'AAA
Cuts at: 3068 3068
Size: 5764
DraIII CAC_nnn'GTG
Cuts at: 2334 2334
Size: 5764
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4771 809
Size: 507 3455 1802
Fragments arranged by size:
3455 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2277 2667 3211
Size: 54 1425 50 275 18 447 390 544
Cuts at: 3211 4100 5729 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 544 447 390 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3211 1537
Size: 275 1399 4090
Fragments arranged by size:
4090 1399 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3694 1353
Size: 2341 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4351 4725 4871 387
Size: 1064 138 2762 374 146 1280
Fragments arranged by size:
2762 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2171 2210 2544 3015 3827 4424 5211
Size: 393 539 39 334 471 812 597 787
Cuts at: 5211 5479 1239
Size: 268 1524
Fragments arranged by size:
1524 812 787 597 539 471 393 334
268 39
EcoNI CCTnn'n_nnAGG
Cuts at: 2225 2986 2225
Size: 761 5003
EcoRI G'AATT_C
Cuts at: 2895 3073 3451 2895
Size: 178 378 5208
Fragments arranged by size:
5208 378 178
FspI TGC'GCA
Cuts at: 4270 4270
Size: 5764
HaeII r_GCGC'y
Cuts at: 1678 2848 4911 1678
Size: 1170 2063 2531
Fragments arranged by size:
2531 2063 1170
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2026 2058 2161 2193 2452
Size: 32 135 32 454 32 103 32 259
Cuts at: 2452 2484 2594 2626 2690 2722 4405 4437 4479
Size: 32 110 32 64 32 1683 32 42
Cuts at: 4479 4511 1373
Size: 32 2626
Fragments arranged by size:
2626 1683 454 259 135 110 103 64
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3108 3354 669
Size: 988 281 1170 246 3079
Fragments arranged by size:
3079 1170 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5764
HpaI GTT'AAC
Cuts at: 3354 3354
Size: 5764
KasI G'GCGC_C
Cuts at: 2844 2844
Size: 5764
KpnI G_GTAC'C
Cuts at: 3089 3089
Size: 5764
MfeI C'AATT_G
Cuts at: 3363 3363
Size: 5764
MluI A'CGCG_T
Cuts at: 5640 5640
Size: 5764
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4878 5062 1305
Size: 3573 184 2007
Fragments arranged by size:
3573 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3942
Fragments arranged by size:
3942 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2355 2369 3722 4081 4240 518
Size: 648 1189 14 1353 359 159 2042
Fragments arranged by size:
2042 1353 1189 648 359 159 14
MspA1I CmG'CkG
Cuts at: 1606 2380 2547 2851 3180 3857 5005 5250 1606
Size: 774 167 304 329 677 1148 245 2120
Fragments arranged by size:
2120 1148 774 677 329 304 245 167
NaeI GCC'GGC
Cuts at: 2671 2671
Size: 5764
NarI GG'CG_CC
Cuts at: 2845 2845
Size: 5764
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4273
Fragments arranged by size:
4273 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5764
NgoMIV G'CCGG_C
Cuts at: 2669 2669
Size: 5764
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5764
NotI GC'GGCC_GC
Cuts at: 3211 3211
Size: 5764
NspI r_CATG'y
Cuts at: 1132 1229 3122 4667 1132
Size: 97 1893 1545 2229
Fragments arranged by size:
2229 1893 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3020 1348
Size: 1672 4092
PciI A'CATG_T
Cuts at: 4663 4663
Size: 5764
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5764
PfoI T'CCnGG_A
Cuts at: 2463 2857 2463
Size: 394 5370
PmeI GTTT'AAAC
Cuts at: 3068 3068
Size: 5764
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3833 3865 5346 5378 3833
Size: 32 1481 32 4219
Fragments arranged by size:
4219 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2866 1613
Size: 1253 4511
PsiI TTA'TAA
Cuts at: 3334 3334
Size: 5764
PstI C_TGCA'G
Cuts at: 830 2193 2250 2893 3116 830
Size: 1363 57 643 223 3478
Fragments arranged by size:
3478 1363 643 223 57
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5764
PvuII CAG'CTG
Cuts at: 2547 2851 2547
Size: 304 5460
SalI G'TCGA_C
Cuts at: 1936 3106 1936
Size: 1170 4594
SbfI CC_TGCA'GG
Cuts at: 3116 3116
Size: 5764
ScaI AGT'ACT
Cuts at: 4012 5724 4012
Size: 1712 4052
SexAI A'CCwGG_T
Cuts at: 2130 2343 2130
Size: 213 5551
SfcI C'TryA_G
Cuts at: 826 1045 2189 2246 2889 3112 4247 4928 5119
Size: 219 1144 57 643 223 1135 681 191
Cuts at: 5119 826
Size: 1471
Fragments arranged by size:
1471 1144 1135 681 643 223 219 191
57
SfoI GGC'GCC
Cuts at: 2846 2846
Size: 5764
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5764
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5764
SmaI CCC'GGG
Cuts at: 1872 3091 1872
Size: 1219 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2864 3868 4769 5031 820
Size: 197 926 921 1004 901 262 1553
Fragments arranged by size:
1553 1004 926 921 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3555 493
Size: 3062 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5764
SphI G_CATG'C
Cuts at: 1132 3122 1132
Size: 1990 3774
SspI AAT'ATT
Cuts at: 5 52 3688 5
Size: 47 3636 2081
Fragments arranged by size:
3636 2081 47
StuI AGG'CCT
Cuts at: 1517 2810 1517
Size: 1293 4471
StyI C'CwwG_G
Cuts at: 513 1065 2004 2393 3203 513
Size: 552 939 389 810 3074
Fragments arranged by size:
3074 939 810 552 389
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3033 3791 3808 3961 4146 4591 325
Size: 1255 1453 758 17 153 185 445 1498
Fragments arranged by size:
1498 1453 1255 758 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4010 5722 96
Size: 274 80 33 51 1443 2033 1712 138
Fragments arranged by size:
2033 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2318 2350 2683 2715 5724 5756 2318
Size: 32 333 32 3009 32 2326
Fragments arranged by size:
3009 2326 333 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5764
XbaI T'CTAG_A
Cuts at: 3100 3100
Size: 5764
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2855 2855
Size: 5764
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5764
XmaI C'CCGG_G
Cuts at: 1870 3089 1870
Size: 1219 4545
XmnI GAAnn'nnTTC
Cuts at: 2930 3893 2930
Size: 963 4801
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3572 276
Size: 53 83 186 936 2038 2468
Fragments arranged by size:
2468 2038 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BplI BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoO109I EcoRV
FalI FseI FspAI NruI NsiI PacI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SfiI SrfI SwaI