Restriction map for
pFN21AE2225
(Circular) (Six-base) MAPSORT of: pFN21AE2225.seq Check: 8623 from: 1 to: 6121
pFN21AE2225 6121 bp
With 182 enzymes: SgfI *
July 6, 2012 17:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3931 278
Size: 53 83 186 936 2395 2468
Fragments arranged by size:
2468 2395 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3464 1937
Size: 1527 4594
Acc65I G'GTAC_C
Cuts at: 3442 3442
Size: 6121
AclI AA'CG_TT
Cuts at: 1563 4248 4621 1563
Size: 2685 373 3063
Fragments arranged by size:
3063 2685 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4184 5568 5836 1632
Size: 2552 1384 268 1917
Fragments arranged by size:
2552 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5924
AflIII A'CryG_T
Cuts at: 1203 5020 5997 1203
Size: 3817 977 1327
Fragments arranged by size:
3817 1327 977
AgeI A'CCGG_T
Cuts at: 4952 4952
Size: 6121
AhdI GACnn_n'nnGTC
Cuts at: 4850 4850
Size: 6121
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6121
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6121
AlwNI CAG_nnn'CTG
Cuts at: 3864 5436 3864
Size: 1572 4549
ApaLI G'TGCA_C
Cuts at: 4178 5334 4178
Size: 1156 4965
ApoI r'AATT_y
Cuts at: 1493 2258 3095 3334 3377 3430 3656 3808 5917
Size: 765 837 239 43 53 226 152 2109
Cuts at: 5917 1493
Size: 1697
Fragments arranged by size:
2109 1697 837 765 239 226 152 53
43
AseI AT'TA_AT
Cuts at: 160 1032 3227 4675 160
Size: 872 2195 1448 1606
Fragments arranged by size:
2195 1606 1448 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6121
AvaI C'yCGr_G
Cuts at: 1870 1943 3446 1870
Size: 73 1503 4545
Fragments arranged by size:
4545 1503 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2006 2039 2670 2703 2006
Size: 33 631 33 5424
Fragments arranged by size:
5424 631 33 33
BamHI G'GATC_C
Cuts at: 3451 3818 3451
Size: 367 5754
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3442 4897 618
Size: 325 217 627 1655 1455 1842
Fragments arranged by size:
1842 1655 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3440 1391
Size: 2049 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2963 928
Size: 971 1064 4086
Fragments arranged by size:
4086 1064 971
BbvCI CC'TCA_GC
Cuts at: 3276 3276
Size: 6121
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4312 4346 1903
Size: 34 2375 34 3678
Fragments arranged by size:
3678 2375 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2671 4013 5223 2671
Size: 1342 1210 3569
Fragments arranged by size:
3569 1342 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6121
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4732 136
Size: 107 122 71 631 605 3060 1525
Fragments arranged by size:
3060 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2350 6116 2350
Size: 3766 2355
BlpI GC'TnA_GC
Cuts at: 3538 3538
Size: 6121
Bme1580I G_kGCm'C
Cuts at: 1790 3243 4182 5338 1790
Size: 1453 939 1156 2573
Fragments arranged by size:
2573 1453 1156 939
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6121
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3079 3158 3852
Size: 416 53 83 399 24 1662 79 694
Cuts at: 3852 4810 442
Size: 958 1753
Fragments arranged by size:
1753 1662 958 694 416 399 83 79
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6121
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2193 2292 4781 1239
Size: 954 99 2489 2579
Fragments arranged by size:
2579 2489 954 99
Bpu10I CC'TnA_GC
Cuts at: 3276 3276
Size: 6121
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4246 5111 5409 4246
Size: 865 298 4958
Fragments arranged by size:
4958 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2516 4784 882
Size: 617 1017 2268 2219
Fragments arranged by size:
2268 2219 1017 617
BsaAI yAC'GTr
Cuts at: 493 1204 3912 493
Size: 711 2708 2702
Fragments arranged by size:
2708 2702 711
BsaBI GATnn'nnATC
Cuts at: 6115 6115
Size: 6121
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2586 3928 4310 4945
Size: 53 83 186 936 1053 1342 382 635
Cuts at: 4945 275
Size: 1451
Fragments arranged by size:
1451 1342 1053 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4554 4952 5226 5373 5784 4554
Size: 398 274 147 411 4891
Fragments arranged by size:
4891 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3084 3114 1178
Size: 30 165 30 1681 30 4185
Fragments arranged by size:
4185 1681 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2158 1186
Size: 972 5149
BseYI C'CCAG_C
Cuts at: 2030 5324 5820 2030
Size: 3294 496 2331
Fragments arranged by size:
3294 2331 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5548
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3571 4332 5360 1540
Size: 275 186 1570 761 1028 2301
Fragments arranged by size:
2301 1570 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3207 4182 4267 5338 3207
Size: 975 85 1071 3990
Fragments arranged by size:
3990 1071 975 85
BsmI GAATG_Cn'
Cuts at: 2330 3193 3632 3725 2330
Size: 863 439 93 4726
Fragments arranged by size:
4726 863 439 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3207 3243 3440 4182 4267 5338 1391
Size: 399 1417 36 197 742 85 1071 2174
Fragments arranged by size:
2174 1417 1071 742 399 197 85 36
BspEI T'CCGG_A
Cuts at: 5784 5784
Size: 6121
BspHI T'CATG_A
Cuts at: 2092 4010 5740 2092
Size: 1918 1730 2473
Fragments arranged by size:
2473 1918 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2778 3476 844
Size: 1934 698 3489
Fragments arranged by size:
3489 1934 698
BsrBI CCG'CTC
Cuts at: 1394 1823 4008 1394
Size: 429 2185 3507
Fragments arranged by size:
3507 2185 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3370 4616 4790 65
Size: 1183 2122 1246 174 1396
Fragments arranged by size:
2122 1396 1246 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4765 4952 1700
Size: 110 2955 187 2869
Fragments arranged by size:
2955 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6121
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6121
BssSI C'ACGA_G
Cuts at: 4181 5193 4181
Size: 1012 5109
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2192 794
Size: 1398 4723
BstBI TT'CG_AA
Cuts at: 4925 4925
Size: 6121
BstEII G'GTnAC_C
Cuts at: 1184 2986 1184
Size: 1802 4319
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 6121
BstYI r'GATC_y
Cuts at: 1972 2350 2486 2863 3451 3818 4203 4220 5672
Size: 378 136 377 588 367 385 17 1452
Cuts at: 5672 5758 5770 6116 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 588 385 378 377 367 346
136 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2870 513
Size: 552 1805 3764
Fragments arranged by size:
3764 1805 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5382
Fragments arranged by size:
5382 683 56
BtsI GCAGTG_nn'
Cuts at: 3637 4431 4451 3637
Size: 794 20 5307
Fragments arranged by size:
5307 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6121
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6086
DraI TTT'AAA
Cuts at: 3285 3425 3285
Size: 140 5981
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5128 809
Size: 507 3812 1802
Fragments arranged by size:
3812 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3568 4457 6086
Size: 54 1425 50 275 18 1738 889 1629
Cuts at: 6086 8
Size: 43
Fragments arranged by size:
1738 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3568 1537
Size: 275 1756 4090
Fragments arranged by size:
4090 1756 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2151 2209 2667 3255 4051 1353
Size: 798 58 458 588 796 3423
Fragments arranged by size:
3423 798 796 588 458 58
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4708 5082 5228 387
Size: 1064 138 3119 374 146 1280
Fragments arranged by size:
3119 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2193 2292 4184 4781 5568 5836 1239
Size: 393 561 99 1892 597 787 268 1524
Fragments arranged by size:
1892 1524 787 597 561 393 268 99
EcoRI G'AATT_C
Cuts at: 3430 3808 3430
Size: 378 5743
FspI TGC'GCA
Cuts at: 4627 4627
Size: 6121
HaeII r_GCGC'y
Cuts at: 1678 5268 1678
Size: 3590 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2059 2091 2147 2179 2666
Size: 32 135 32 487 32 56 32 487
Cuts at: 2666 2698 4762 4794 4836 4868 1373
Size: 32 2064 32 42 32 2626
Fragments arranged by size:
2626 2064 487 487 135 56 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3465 3711 669
Size: 988 281 1527 246 3079
Fragments arranged by size:
3079 1527 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6121
HpaI GTT'AAC
Cuts at: 3711 3711
Size: 6121
KpnI G_GTAC'C
Cuts at: 3446 3446
Size: 6121
MfeI C'AATT_G
Cuts at: 2663 3720 2663
Size: 1057 5064
MluI A'CGCG_T
Cuts at: 5997 5997
Size: 6121
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3329 5235 5419 1305
Size: 2024 1906 184 2007
Fragments arranged by size:
2024 2007 1906 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4299
Fragments arranged by size:
4299 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 4079 4438 4597 518
Size: 648 843 2070 359 159 2042
Fragments arranged by size:
2070 2042 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2030 2312 3537 4214 5362 5607 1606
Size: 424 282 1225 677 1148 245 2120
Fragments arranged by size:
2120 1225 1148 677 424 282 245
NcoI C'CATG_G
Cuts at: 513 1065 2870 513
Size: 552 1805 3764
Fragments arranged by size:
3764 1805 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6121
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6121
NotI GC'GGCC_GC
Cuts at: 3568 3568
Size: 6121
NspI r_CATG'y
Cuts at: 1132 1229 3389 3479 5024 1132
Size: 97 2160 90 1545 2229
Fragments arranged by size:
2229 2160 1545 97 90
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6121
PciI A'CATG_T
Cuts at: 5020 5020
Size: 6121
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6121
PfoI T'CCnGG_A
Cuts at: 2502 2502
Size: 6121
PmeI GTTT'AAAC
Cuts at: 3425 3425
Size: 6121
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4190 4222 5703 5735 4190
Size: 32 1481 32 4576
Fragments arranged by size:
4576 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6121
PsiI TTA'TAA
Cuts at: 3017 3691 3017
Size: 674 5447
PstI C_TGCA'G
Cuts at: 830 2594 3473 830
Size: 1764 879 3478
Fragments arranged by size:
3478 1764 879
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6121
PvuII CAG'CTG
Cuts at: 2030 2312 2030
Size: 282 5839
SalI G'TCGA_C
Cuts at: 1936 3463 1936
Size: 1527 4594
SbfI CC_TGCA'GG
Cuts at: 3473 3473
Size: 6121
ScaI AGT'ACT
Cuts at: 4369 6081 4369
Size: 1712 4409
SfcI C'TryA_G
Cuts at: 826 1045 2590 2833 3469 4604 5285 5476 826
Size: 219 1545 243 636 1135 681 191 1471
Fragments arranged by size:
1545 1471 1135 681 636 243 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6121
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6121
SmaI CCC'GGG
Cuts at: 1872 3448 1872
Size: 1576 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4225 5126 5388 820
Size: 197 926 2282 901 262 1553
Fragments arranged by size:
2282 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3912 493
Size: 3419 2702
SpeI A'CTAG_T
Cuts at: 152 3353 152
Size: 3201 2920
SphI G_CATG'C
Cuts at: 1132 3479 1132
Size: 2347 3774
SspI AAT'ATT
Cuts at: 5 52 3314 4045 5
Size: 47 3262 731 2081
Fragments arranged by size:
3262 2081 731 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6121
StyI C'CwwG_G
Cuts at: 513 1065 2870 3560 513
Size: 552 1805 690 3074
Fragments arranged by size:
3074 1805 690 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2022 2974 4148 4165 4318 4503 4948
Size: 1255 442 952 1174 17 153 185 445
Cuts at: 4948 325
Size: 1498
Fragments arranged by size:
1498 1255 1174 952 445 442 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3245 4367 6079
Size: 274 80 33 51 1443 1268 1122 1712
Cuts at: 6079 96
Size: 138
Fragments arranged by size:
1712 1443 1268 1122 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3084 3116 6081 6113 3084
Size: 32 2965 32 3092
Fragments arranged by size:
3092 2965 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6121
XbaI T'CTAG_A
Cuts at: 3457 3457
Size: 6121
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2027 2027
Size: 6121
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6121
XmaI C'CCGG_G
Cuts at: 1870 3446 1870
Size: 1576 4545
XmnI GAAnn'nnTTC
Cuts at: 4250 4250
Size: 6121
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3929 276
Size: 53 83 186 936 2395 2468
Fragments arranged by size:
2468 2395 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI