Restriction map for
pFN21AE2223
(Circular) (Six-base) MAPSORT of: pFN21AE2223.seq Check: 9107 from: 1 to: 5719
pFN21AE2223 5719 bp
With 182 enzymes: SgfI *
August 16, 2010 14:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2694 2694
Size: 5719
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3529 278
Size: 53 83 186 936 1993 2468
Fragments arranged by size:
2468 1993 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3062 1937
Size: 1125 4594
Acc65I G'GTAC_C
Cuts at: 3040 3040
Size: 5719
AclI AA'CG_TT
Cuts at: 1563 3846 4219 1563
Size: 2283 373 3063
Fragments arranged by size:
3063 2283 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2065 2909 3782 5166 5434 1632
Size: 433 844 873 1384 268 1917
Fragments arranged by size:
1917 1384 873 844 433 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5522
AflIII A'CryG_T
Cuts at: 1203 4618 5595 1203
Size: 3415 977 1327
Fragments arranged by size:
3415 1327 977
AgeI A'CCGG_T
Cuts at: 4550 4550
Size: 5719
AhdI GACnn_n'nnGTC
Cuts at: 4448 4448
Size: 5719
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5719
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5719
AlwNI CAG_nnn'CTG
Cuts at: 2145 2242 2919 3462 5034 2145
Size: 97 677 543 1572 2830
Fragments arranged by size:
2830 1572 677 543 97
ApaI G_GGCC'C
Cuts at: 2035 2699 2035
Size: 664 5055
ApaLI G'TGCA_C
Cuts at: 3776 4932 3776
Size: 1156 4563
ApoI r'AATT_y
Cuts at: 1493 3028 3254 3406 5515 1493
Size: 1535 226 152 2109 1697
Fragments arranged by size:
2109 1697 1535 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4273 160
Size: 872 3241 1606
Fragments arranged by size:
3241 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5719
AvaI C'yCGr_G
Cuts at: 1870 1943 2067 2184 3044 1870
Size: 73 124 117 860 4545
Fragments arranged by size:
4545 860 124 117 73
BamHI G'GATC_C
Cuts at: 2471 3049 3416 2471
Size: 578 367 4774
Fragments arranged by size:
4774 578 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2383 2845 2898 3040 4495
Size: 325 217 627 596 462 53 142 1455
Cuts at: 4495 618
Size: 1842
Fragments arranged by size:
1842 1455 627 596 462 325 217 142
53
BanII G_rGCy'C
Cuts at: 1391 2035 2370 2699 3038 1391
Size: 644 335 329 339 4072
Fragments arranged by size:
4072 644 339 335 329
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2725 2787 928
Size: 971 826 62 3860
Fragments arranged by size:
3860 971 826 62
BbvCI CC'TCA_GC
Cuts at: 2528 2528
Size: 5719
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3910 3944 1903
Size: 34 1973 34 3678
Fragments arranged by size:
3678 1973 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2183 2303 3611 4821 2183
Size: 120 1308 1210 3081
Fragments arranged by size:
3081 1308 1210 120
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5719
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4330 136
Size: 107 122 71 631 605 2658 1525
Fragments arranged by size:
2658 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5714 5714
Size: 5719
BlpI GC'TnA_GC
Cuts at: 2369 2537 3136 2369
Size: 168 599 4952
Fragments arranged by size:
4952 599 168
Bme1580I G_kGCm'C
Cuts at: 1790 2035 2388 2699 3780 4936 1790
Size: 245 353 311 1081 1156 2573
Fragments arranged by size:
2573 1156 1081 353 311 245
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5719
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2160 2384 3450
Size: 416 53 83 399 24 743 224 1066
Cuts at: 3450 4408 442
Size: 958 1753
Fragments arranged by size:
1753 1066 958 743 416 399 224 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5719
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2516 4379 1239
Size: 1277 1863 2579
Fragments arranged by size:
2579 1863 1277
Bpu10I CC'TnA_GC
Cuts at: 2528 2528
Size: 5719
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3844 4709 5007 3844
Size: 865 298 4556
Fragments arranged by size:
4556 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4382 882
Size: 617 2883 2219
Fragments arranged by size:
2883 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3510 493
Size: 711 2306 2702
Fragments arranged by size:
2702 2306 711
BsaBI GATnn'nnATC
Cuts at: 2642 5713 2642
Size: 3071 2648
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3526 3908 4543 275
Size: 53 83 186 936 1993 382 635 1451
Fragments arranged by size:
1993 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3003 4152 4550 4824 4971 5382 3003
Size: 1149 398 274 147 411 3340
Fragments arranged by size:
3340 1149 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2047 2077 2838 2868 1178
Size: 30 165 30 644 30 761 30 4029
Fragments arranged by size:
4029 761 644 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2068 2822 1186
Size: 882 754 4083
Fragments arranged by size:
4083 882 754
BseYI C'CCAG_C
Cuts at: 2073 2111 2262 4922 5418 2073
Size: 38 151 2660 496 2374
Fragments arranged by size:
2660 2374 496 151 38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2354 1163
Size: 573 618 4528
Fragments arranged by size:
4528 618 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3169 3930 4958 1540
Size: 275 186 1168 761 1028 2301
Fragments arranged by size:
2301 1168 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3780 3865 4936 3780
Size: 85 1071 4563
Fragments arranged by size:
4563 1071 85
BsmI GAATG_Cn'
Cuts at: 2028 2614 3230 3323 2028
Size: 586 616 93 4424
Fragments arranged by size:
4424 616 586 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2035 2370 2388 2699 3038 3780 3865
Size: 399 245 335 18 311 339 742 85
Cuts at: 3865 4936 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 339 335 311 245
85 18
BspEI T'CCGG_A
Cuts at: 3003 5382 3003
Size: 2379 3340
BspHI T'CATG_A
Cuts at: 3608 5338 3608
Size: 1730 3989
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2694 3074 844
Size: 1850 380 3489
Fragments arranged by size:
3489 1850 380
BsrBI CCG'CTC
Cuts at: 1394 1823 3606 1394
Size: 429 1783 3507
Fragments arranged by size:
3507 1783 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2562 4214 4388 65
Size: 1183 1314 1652 174 1396
Fragments arranged by size:
1652 1396 1314 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2248 2329 2931 4363 4550 1700
Size: 110 438 81 602 1432 187 2869
Fragments arranged by size:
2869 1432 602 438 187 110 81
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5719
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5719
BssSI C'ACGA_G
Cuts at: 3779 4791 3779
Size: 1012 4707
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2326 794
Size: 1532 4187
BstBI TT'CG_AA
Cuts at: 4523 4523
Size: 5719
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5719
BstXI CCAn_nnnn'nTGG
Cuts at: 2134 2150 2134
Size: 16 5703
BstYI r'GATC_y
Cuts at: 1972 2471 2766 3049 3416 3801 3818 5270 5356
Size: 499 295 283 367 385 17 1452 86
Cuts at: 5356 5368 5714 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 499 385 367 346 295 283
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2178 2243 2375 2178
Size: 65 132 5522
Fragments arranged by size:
5522 132 65
BtgI C'CryG_G
Cuts at: 513 1065 2004 2402 2700 2787 513
Size: 552 939 398 298 87 3445
Fragments arranged by size:
3445 939 552 398 298 87
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4980
Fragments arranged by size:
4980 683 56
BtsI GCAGTG_nn'
Cuts at: 3235 4029 4049 3235
Size: 794 20 4905
Fragments arranged by size:
4905 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5719
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5684
DraI TTT'AAA
Cuts at: 3023 3023
Size: 5719
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2711 4726 809
Size: 507 1395 2015 1802
Fragments arranged by size:
2015 1802 1395 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2405 3166 4055
Size: 54 1425 50 275 18 575 761 889
Cuts at: 4055 5684 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 761 575 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3166 1537
Size: 275 1354 4090
Fragments arranged by size:
4090 1354 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2159 2436 3649 1353
Size: 806 277 1213 3423
Fragments arranged by size:
3423 1213 806 277
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4306 4680 4826 387
Size: 1064 138 2717 374 146 1280
Fragments arranged by size:
2717 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2065 2516 2909 3782 4379 5166 5434
Size: 393 433 451 393 873 597 787 268
Cuts at: 5434 1239
Size: 1524
Fragments arranged by size:
1524 873 787 597 451 433 393 393
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2668 2668
Size: 5719
EcoO109I rG'GnC_Cy
Cuts at: 2032 2457 2695 2696 2985 2032
Size: 425 238 1 289 4766
Fragments arranged by size:
4766 425 289 238 1
EcoRI G'AATT_C
Cuts at: 3028 3406 3028
Size: 378 5341
FspI TGC'GCA
Cuts at: 2966 4225 2966
Size: 1259 4460
HaeII r_GCGC'y
Cuts at: 1678 4866 1678
Size: 3188 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2296 2328 2648 2680 4360
Size: 32 135 32 724 32 320 32 1680
Cuts at: 4360 4392 4434 4466 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1680 724 320 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3063 3309 669
Size: 988 281 1125 246 3079
Fragments arranged by size:
3079 1125 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5719
HpaI GTT'AAC
Cuts at: 3309 3309
Size: 5719
KpnI G_GTAC'C
Cuts at: 3044 3044
Size: 5719
MfeI C'AATT_G
Cuts at: 3318 3318
Size: 5719
MluI A'CGCG_T
Cuts at: 5595 5595
Size: 5719
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4833 5017 1305
Size: 3528 184 2007
Fragments arranged by size:
3528 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3897
Fragments arranged by size:
3897 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2567 2607 3677 4036 4195 518
Size: 648 1401 40 1070 359 159 2042
Fragments arranged by size:
2042 1401 1070 648 359 159 40
MspA1I CmG'CkG
Cuts at: 1606 2404 2755 2789 2919 3135 3812 4960 5205
Size: 798 351 34 130 216 677 1148 245
Cuts at: 5205 1606
Size: 2120
Fragments arranged by size:
2120 1148 798 677 351 245 216 130
34
NaeI GCC'GGC
Cuts at: 2250 2933 2250
Size: 683 5036
NcoI C'CATG_G
Cuts at: 513 1065 2004 2700 513
Size: 552 939 696 3532
Fragments arranged by size:
3532 939 696 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5719
NgoMIV G'CCGG_C
Cuts at: 2248 2931 2248
Size: 683 5036
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5719
NotI GC'GGCC_GC
Cuts at: 3166 3166
Size: 5719
NspI r_CATG'y
Cuts at: 1132 1229 3077 4622 1132
Size: 97 1848 1545 2229
Fragments arranged by size:
2229 1848 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2048 2691 1348
Size: 700 643 4376
Fragments arranged by size:
4376 700 643
PciI A'CATG_T
Cuts at: 4618 4618
Size: 5719
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2467 2746 2836 1108
Size: 1359 279 90 3991
Fragments arranged by size:
3991 1359 279 90
PfoI T'CCnGG_A
Cuts at: 2295 2295
Size: 5719
PmeI GTTT'AAAC
Cuts at: 3023 3023
Size: 5719
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3788 3820 5301 5333 3788
Size: 32 1481 32 4174
Fragments arranged by size:
4174 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2457 2457
Size: 5719
PshAI GACnn'nnGTC
Cuts at: 1613 2153 1613
Size: 540 5179
PsiI TTA'TAA
Cuts at: 3289 3289
Size: 5719
PspOMI G'GGCC_C
Cuts at: 2031 2695 2031
Size: 664 5055
PstI C_TGCA'G
Cuts at: 830 3071 830
Size: 2241 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5719
PvuII CAG'CTG
Cuts at: 2755 2919 2755
Size: 164 5555
SacII CC_GC'GG
Cuts at: 2405 2790 2405
Size: 385 5334
SalI G'TCGA_C
Cuts at: 1936 3061 1936
Size: 1125 4594
SbfI CC_TGCA'GG
Cuts at: 3071 3071
Size: 5719
ScaI AGT'ACT
Cuts at: 3967 5679 3967
Size: 1712 4007
SfcI C'TryA_G
Cuts at: 826 1045 2285 3067 4202 4883 5074 826
Size: 219 1240 782 1135 681 191 1471
Fragments arranged by size:
1471 1240 1135 782 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5719
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5719
SmaI CCC'GGG
Cuts at: 1872 2069 2186 3046 1872
Size: 197 117 860 4545
Fragments arranged by size:
4545 860 197 117
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3823 4724 4986 820
Size: 197 926 1880 901 262 1553
Fragments arranged by size:
1880 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3510 493
Size: 3017 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5719
SphI G_CATG'C
Cuts at: 1132 3077 1132
Size: 1945 3774
SspI AAT'ATT
Cuts at: 5 52 3643 5
Size: 47 3591 2081
Fragments arranged by size:
3591 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5719
StyI C'CwwG_G
Cuts at: 513 1065 2004 2218 2344 2700 2841 2894 3158
Size: 552 939 214 126 356 141 53 264
Cuts at: 3158 513
Size: 3074
Fragments arranged by size:
3074 939 552 356 264 214 141 126
53
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2422 3746 3763 3916 4101 4546 325
Size: 1255 842 1324 17 153 185 445 1498
Fragments arranged by size:
1498 1324 1255 842 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2199 2337 3965
Size: 274 80 33 51 1443 222 138 1628
Cuts at: 3965 5677 96
Size: 1712 138
Fragments arranged by size:
1712 1628 1443 274 222 138 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2838 2870 5679 5711 2838
Size: 32 2809 32 2846
Fragments arranged by size:
2846 2809 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5719
XbaI T'CTAG_A
Cuts at: 3055 3055
Size: 5719
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5719
XmaI C'CCGG_G
Cuts at: 1870 2067 2184 3044 1870
Size: 197 117 860 4545
Fragments arranged by size:
4545 860 197 117
XmnI GAAnn'nnTTC
Cuts at: 3848 3848
Size: 5719
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3527 276
Size: 53 83 186 936 1993 2468
Fragments arranged by size:
2468 1993 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI
FspAI KasI NarI NruI NsiI PacI PmlI PspXI
PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI