Restriction map for
pFN21AE2220
(Circular) (Six-base) MAPSORT of: pFN21AE2220.seq Check: 460 from: 1 to: 5227
pFN21AE2220 5227 bp
With 182 enzymes: SgfI *
August 16, 2010 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3037 278
Size: 53 83 186 936 1501 2468
Fragments arranged by size:
2468 1501 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2570 1937
Size: 633 4594
Acc65I G'GTAC_C
Cuts at: 2548 2548
Size: 5227
AclI AA'CG_TT
Cuts at: 1563 3354 3727 1563
Size: 1791 373 3063
Fragments arranged by size:
3063 1791 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3290 4674 4942 1632
Size: 1658 1384 268 1917
Fragments arranged by size:
1917 1658 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5030
AflIII A'CryG_T
Cuts at: 1203 4126 5103 1203
Size: 2923 977 1327
Fragments arranged by size:
2923 1327 977
AgeI A'CCGG_T
Cuts at: 4058 4058
Size: 5227
AhdI GACnn_n'nnGTC
Cuts at: 3956 3956
Size: 5227
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5227
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5227
AlwNI CAG_nnn'CTG
Cuts at: 2100 2970 4542 2100
Size: 870 1572 2785
Fragments arranged by size:
2785 1572 870
ApaLI G'TGCA_C
Cuts at: 3284 4440 3284
Size: 1156 4071
ApoI r'AATT_y
Cuts at: 1493 2536 2762 2914 5023 1493
Size: 1043 226 152 2109 1697
Fragments arranged by size:
2109 1697 1043 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3781 160
Size: 872 2749 1606
Fragments arranged by size:
2749 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5227
AvaI C'yCGr_G
Cuts at: 1870 1943 2552 1870
Size: 73 609 4545
Fragments arranged by size:
4545 609 73
BamHI G'GATC_C
Cuts at: 2557 2924 2557
Size: 367 4860
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2463 2548 4003 618
Size: 325 217 627 676 85 1455 1842
Fragments arranged by size:
1842 1455 676 627 325 217 85
BanII G_rGCy'C
Cuts at: 1391 2546 1391
Size: 1155 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2298 2331 928
Size: 971 399 33 3824
Fragments arranged by size:
3824 971 399 33
BbvCI CC'TCA_GC
Cuts at: 2219 2357 2441 2219
Size: 138 84 5005
Fragments arranged by size:
5005 138 84
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3418 3452 1903
Size: 34 1481 34 3678
Fragments arranged by size:
3678 1481 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3119 4329 3119
Size: 1210 4017
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5227
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3838 136
Size: 107 122 71 631 605 2166 1525
Fragments arranged by size:
2166 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5222 5222
Size: 5227
BlpI GC'TnA_GC
Cuts at: 2644 2644
Size: 5227
Bme1580I G_kGCm'C
Cuts at: 1790 3288 4444 1790
Size: 1498 1156 2573
Fragments arranged by size:
2573 1498 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5227
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2482 2958 3916
Size: 416 53 83 399 24 1065 476 958
Cuts at: 3916 442
Size: 1753
Fragments arranged by size:
1753 1065 958 476 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5227
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2405 3887 1239
Size: 1166 1482 2579
Fragments arranged by size:
2579 1482 1166
Bpu10I CC'TnA_GC
Cuts at: 2219 2357 2441 2490 2219
Size: 138 84 49 4956
Fragments arranged by size:
4956 138 84 49
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3352 4217 4515 3352
Size: 865 298 4064
Fragments arranged by size:
4064 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3890 882
Size: 617 2391 2219
Fragments arranged by size:
2391 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2380 3018 493
Size: 711 1176 638 2702
Fragments arranged by size:
2702 1176 711 638
BsaBI GATnn'nnATC
Cuts at: 5221 5221
Size: 5227
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3034 3416 4051 275
Size: 53 83 186 936 1501 382 635 1451
Fragments arranged by size:
1501 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3660 4058 4332 4479 4890 3660
Size: 398 274 147 411 3997
Fragments arranged by size:
3997 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5002
Fragments arranged by size:
5002 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5227
BseYI C'CCAG_C
Cuts at: 2333 2394 4430 4926 2333
Size: 61 2036 496 2634
Fragments arranged by size:
2634 2036 496 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4654
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2677 3438 4466 1540
Size: 275 186 676 761 1028 2301
Fragments arranged by size:
2301 1028 761 676 275 186
BsiHKAI G_wGCw'C
Cuts at: 3288 3373 4444 3288
Size: 85 1071 4071
Fragments arranged by size:
4071 1071 85
BsmI GAATG_Cn'
Cuts at: 2738 2831 2738
Size: 93 5134
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2546 3288 3373 4444 1391
Size: 399 756 742 85 1071 2174
Fragments arranged by size:
2174 1071 756 742 399 85
BspEI T'CCGG_A
Cuts at: 4890 4890
Size: 5227
BspHI T'CATG_A
Cuts at: 2307 3116 4846 2307
Size: 809 1730 2688
Fragments arranged by size:
2688 1730 809
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2088 2445 2582 844
Size: 1244 357 137 3489
Fragments arranged by size:
3489 1244 357 137
BsrBI CCG'CTC
Cuts at: 1394 1823 2124 2235 3114 1394
Size: 429 301 111 879 3507
Fragments arranged by size:
3507 879 429 301 111
BsrDI GCAATG_nn'
Cuts at: 65 1248 2213 3722 3896 65
Size: 1183 965 1509 174 1396
Fragments arranged by size:
1509 1396 1183 965 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3871 4058 1700
Size: 110 2061 187 2869
Fragments arranged by size:
2869 2061 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5227
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5227
BssSI C'ACGA_G
Cuts at: 3287 4299 3287
Size: 1012 4215
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2437 794
Size: 1643 3584
BstBI TT'CG_AA
Cuts at: 4031 4031
Size: 5227
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5227
BstXI CCAn_nnnn'nTGG
Cuts at: 2116 2116
Size: 5227
BstYI r'GATC_y
Cuts at: 1972 2364 2557 2924 3309 3326 4778 4864 4876
Size: 392 193 367 385 17 1452 86 12
Cuts at: 4876 5222 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 392 385 367 346 193 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2481 513
Size: 552 939 477 3259
Fragments arranged by size:
3259 939 552 477
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4488
Fragments arranged by size:
4488 683 56
BtsI GCAGTG_nn'
Cuts at: 2743 3537 3557 2743
Size: 794 20 4413
Fragments arranged by size:
4413 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5227
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5192
DraI TTT'AAA
Cuts at: 2531 2531
Size: 5227
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4234 809
Size: 507 2918 1802
Fragments arranged by size:
2918 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2674 3563 5192
Size: 54 1425 50 275 18 844 889 1629
Cuts at: 5192 8
Size: 43
Fragments arranged by size:
1629 1425 889 844 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2674 1537
Size: 275 862 4090
Fragments arranged by size:
4090 862 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3157 1353
Size: 1804 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3814 4188 4334 387
Size: 1064 138 2225 374 146 1280
Fragments arranged by size:
2225 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2405 3290 3887 4674 4942 1239
Size: 393 773 885 597 787 268 1524
Fragments arranged by size:
1524 885 787 773 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2034 2398 2445 2034
Size: 364 47 4816
Fragments arranged by size:
4816 364 47
EcoRI G'AATT_C
Cuts at: 2536 2914 2536
Size: 378 4849
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2035 2035
Size: 5227
FspI TGC'GCA
Cuts at: 3733 3733
Size: 5227
HaeII r_GCGC'y
Cuts at: 1678 4374 1678
Size: 2696 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3868 3900 3942 3974 1373
Size: 32 135 32 2296 32 42 32 2626
Fragments arranged by size:
2626 2296 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2165 2571 2817 669
Size: 988 281 227 406 246 3079
Fragments arranged by size:
3079 988 406 281 246 227
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5227
HpaI GTT'AAC
Cuts at: 2817 2817
Size: 5227
KpnI G_GTAC'C
Cuts at: 2552 2552
Size: 5227
MfeI C'AATT_G
Cuts at: 2826 2826
Size: 5227
MluI A'CGCG_T
Cuts at: 5103 5103
Size: 5227
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4341 4525 1305
Size: 3036 184 2007
Fragments arranged by size:
3036 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3405
Fragments arranged by size:
3405 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2114 3185 3544 3703 518
Size: 648 948 1071 359 159 2042
Fragments arranged by size:
2042 1071 948 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2100 2643 3320 4468 4713 1606
Size: 494 543 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 543 494 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2481 513
Size: 552 939 477 3259
Fragments arranged by size:
3259 939 552 477
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5227
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5227
NotI GC'GGCC_GC
Cuts at: 2674 2674
Size: 5227
NspI r_CATG'y
Cuts at: 1132 1229 2585 4130 1132
Size: 97 1356 1545 2229
Fragments arranged by size:
2229 1545 1356 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5227
PciI A'CATG_T
Cuts at: 4126 4126
Size: 5227
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2383 1108
Size: 1275 3952
PfoI T'CCnGG_A
Cuts at: 2340 2340
Size: 5227
PmeI GTTT'AAAC
Cuts at: 2531 2531
Size: 5227
PmlI CAC'GTG
Cuts at: 2380 2380
Size: 5227
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3296 3328 4809 4841 3296
Size: 32 1481 32 3682
Fragments arranged by size:
3682 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2398 2398
Size: 5227
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5227
PsiI TTA'TAA
Cuts at: 2797 2797
Size: 5227
PstI C_TGCA'G
Cuts at: 830 2085 2579 830
Size: 1255 494 3478
Fragments arranged by size:
3478 1255 494
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5227
SalI G'TCGA_C
Cuts at: 1936 2569 1936
Size: 633 4594
SbfI CC_TGCA'GG
Cuts at: 2579 2579
Size: 5227
ScaI AGT'ACT
Cuts at: 3475 5187 3475
Size: 1712 3515
SfcI C'TryA_G
Cuts at: 826 1045 2081 2575 3710 4391 4582 826
Size: 219 1036 494 1135 681 191 1471
Fragments arranged by size:
1471 1135 1036 681 494 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5227
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5227
SmaI CCC'GGG
Cuts at: 1872 2554 1872
Size: 682 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3331 4232 4494 820
Size: 197 926 1388 901 262 1553
Fragments arranged by size:
1553 1388 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3018 493
Size: 2525 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5227
SphI G_CATG'C
Cuts at: 1132 2585 1132
Size: 1453 3774
SspI AAT'ATT
Cuts at: 5 52 3151 5
Size: 47 3099 2081
Fragments arranged by size:
3099 2081 47
StuI AGG'CCT
Cuts at: 1517 2304 1517
Size: 787 4440
StyI C'CwwG_G
Cuts at: 513 1065 2004 2158 2481 2666 513
Size: 552 939 154 323 185 3074
Fragments arranged by size:
3074 939 552 323 185 154
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3254 3271 3424 3609 4054 325
Size: 1255 1674 17 153 185 445 1498
Fragments arranged by size:
1674 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2454 3473 5185
Size: 274 80 33 51 1443 477 1019 1712
Cuts at: 5185 96
Size: 138
Fragments arranged by size:
1712 1443 1019 477 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5187 5219 5187
Size: 32 5195
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5227
XbaI T'CTAG_A
Cuts at: 2563 2563
Size: 5227
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5227
XmaI C'CCGG_G
Cuts at: 1870 2552 1870
Size: 682 4545
XmnI GAAnn'nnTTC
Cuts at: 3356 3356
Size: 5227
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3035 276
Size: 53 83 186 936 1501 2468
Fragments arranged by size:
2468 1501 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI