Restriction map for
pFN21AE2216
(Circular) (Six-base) MAPSORT of: pFN21AE2216.seq Check: 6729 from: 1 to: 5362
pFN21AE2216 5362 bp
With 182 enzymes: SgfI *
August 16, 2010 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3172 278
Size: 53 83 186 936 1636 2468
Fragments arranged by size:
2468 1636 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2705 1937
Size: 768 4594
Acc65I G'GTAC_C
Cuts at: 2683 2683
Size: 5362
AclI AA'CG_TT
Cuts at: 1563 3489 3862 1563
Size: 1926 373 3063
Fragments arranged by size:
3063 1926 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3425 4809 5077 1632
Size: 1793 1384 268 1917
Fragments arranged by size:
1917 1793 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5165
AflIII A'CryG_T
Cuts at: 1203 2292 2415 4261 5238 1203
Size: 1089 123 1846 977 1327
Fragments arranged by size:
1846 1327 1089 977 123
AgeI A'CCGG_T
Cuts at: 4193 4193
Size: 5362
AhdI GACnn_n'nnGTC
Cuts at: 2081 4091 2081
Size: 2010 3352
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5362
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5362
AlwNI CAG_nnn'CTG
Cuts at: 2179 2281 3105 4677 2179
Size: 102 824 1572 2864
Fragments arranged by size:
2864 1572 824 102
ApaLI G'TGCA_C
Cuts at: 3419 4575 3419
Size: 1156 4206
ApoI r'AATT_y
Cuts at: 1493 2589 2671 2897 3049 5158 1493
Size: 1096 82 226 152 2109 1697
Fragments arranged by size:
2109 1697 1096 226 152 82
AseI AT'TA_AT
Cuts at: 160 1032 3916 160
Size: 872 2884 1606
Fragments arranged by size:
2884 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5362
AvaI C'yCGr_G
Cuts at: 1870 1943 2433 2687 1870
Size: 73 490 254 4545
Fragments arranged by size:
4545 490 254 73
BamHI G'GATC_C
Cuts at: 2692 3059 2692
Size: 367 4995
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2683 4138 618
Size: 325 217 627 896 1455 1842
Fragments arranged by size:
1842 1455 896 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2333 2481 2538 2681 1391
Size: 942 148 57 143 4072
Fragments arranged by size:
4072 942 148 143 57
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4391
BbvCI CC'TCA_GC
Cuts at: 2092 2092
Size: 5362
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2414 2448 3553 3587 1903
Size: 34 477 34 1105 34 3678
Fragments arranged by size:
3678 1105 477 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3254 4464 3254
Size: 1210 4152
BclI T'GATC_A
Cuts at: 1548 2128 1548
Size: 580 4782
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3973 136
Size: 107 122 71 631 605 2301 1525
Fragments arranged by size:
2301 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5357 5357
Size: 5362
BlpI GC'TnA_GC
Cuts at: 2625 2779 2625
Size: 154 5208
Bme1580I G_kGCm'C
Cuts at: 1790 3423 4579 1790
Size: 1633 1156 2573
Fragments arranged by size:
2573 1633 1156
BmgBI CAC'GTC
Cuts at: 1318 2416 1318
Size: 1098 4264
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2025 3093 4051
Size: 416 53 83 399 24 608 1068 958
Cuts at: 4051 442
Size: 1753
Fragments arranged by size:
1753 1068 958 608 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2186 1056
Size: 1130 4232
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2032 2032
Size: 5362
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2303 2347 4022 1239
Size: 1064 44 1675 2579
Fragments arranged by size:
2579 1675 1064 44
Bpu10I CC'TnA_GC
Cuts at: 2092 2092
Size: 5362
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3487 4352 4650 3487
Size: 865 298 4199
Fragments arranged by size:
4199 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2192 2430 4025 882
Size: 617 693 238 1595 2219
Fragments arranged by size:
2219 1595 693 617 238
BsaAI yAC'GTr
Cuts at: 493 1204 2295 3153 493
Size: 711 1091 858 2702
Fragments arranged by size:
2702 1091 858 711
BsaBI GATnn'nnATC
Cuts at: 5356 5356
Size: 5362
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3169 3551 4186 275
Size: 53 83 186 936 1636 382 635 1451
Fragments arranged by size:
1636 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2145 3795 4193 4467 4614 5025 2145
Size: 1650 398 274 147 411 2482
Fragments arranged by size:
2482 1650 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2080 2110 1178
Size: 30 165 30 677 30 4430
Fragments arranged by size:
4430 677 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2252 1186
Size: 1066 4296
BseYI C'CCAG_C
Cuts at: 2106 2584 4565 5061 2106
Size: 478 1981 496 2407
Fragments arranged by size:
2407 1981 496 478
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4789
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2812 3573 4601 1540
Size: 275 186 811 761 1028 2301
Fragments arranged by size:
2301 1028 811 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2538 3423 3508 4579 2538
Size: 885 85 1071 3321
Fragments arranged by size:
3321 1071 885 85
BsmI GAATG_Cn'
Cuts at: 2873 2966 2873
Size: 93 5269
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2333 2481 2538 2681 3423 3508 4579
Size: 399 543 148 57 143 742 85 1071
Cuts at: 4579 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 543 399 148 143 85
57
BspEI T'CCGG_A
Cuts at: 2145 5025 2145
Size: 2880 2482
BspHI T'CATG_A
Cuts at: 3251 4981 3251
Size: 1730 3632
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2717 844
Size: 1873 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3249 1394
Size: 429 1426 3507
Fragments arranged by size:
3507 1426 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2394 3857 4031 65
Size: 1183 1146 1463 174 1396
Fragments arranged by size:
1463 1396 1183 1146 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4006 4193 1700
Size: 110 2196 187 2869
Fragments arranged by size:
2869 2196 187 110
BsrGI T'GTAC_A
Cuts at: 96 2418 96
Size: 2322 3040
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5362
BssSI C'ACGA_G
Cuts at: 3422 4434 3422
Size: 1012 4350
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5362
BstBI TT'CG_AA
Cuts at: 4166 4166
Size: 5362
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5362
BstXI CCAn_nnnn'nTGG
Cuts at: 2194 2194
Size: 5362
BstYI r'GATC_y
Cuts at: 1972 2692 3059 3444 3461 4913 4999 5011 5357
Size: 720 367 385 17 1452 86 12 346
Cuts at: 5357 1972
Size: 1977
Fragments arranged by size:
1977 1452 720 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3871
Fragments arranged by size:
3871 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4623
Fragments arranged by size:
4623 683 56
BtsI GCAGTG_nn'
Cuts at: 2259 2555 2619 2636 2878 3672 3692 2259
Size: 296 64 17 242 794 20 3929
Fragments arranged by size:
3929 794 296 242 64 20 17
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5362
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5327
DraI TTT'AAA
Cuts at: 2666 2666
Size: 5362
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4369 809
Size: 507 3053 1802
Fragments arranged by size:
3053 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2809 3698 5327
Size: 54 1425 50 275 18 979 889 1629
Cuts at: 5327 8
Size: 43
Fragments arranged by size:
1629 1425 979 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2809 1537
Size: 275 997 4090
Fragments arranged by size:
4090 997 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3292 1353
Size: 1939 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3949 4323 4469 387
Size: 1064 138 2360 374 146 1280
Fragments arranged by size:
2360 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2536 2536
Size: 5362
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2303 2347 3425 4022 4809 5077 1239
Size: 393 671 44 1078 597 787 268 1524
Fragments arranged by size:
1524 1078 787 671 597 393 268 44
EcoRI G'AATT_C
Cuts at: 2671 3049 2671
Size: 378 4984
FspI TGC'GCA
Cuts at: 3868 3868
Size: 5362
HaeII r_GCGC'y
Cuts at: 1678 4509 1678
Size: 2831 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2067 2080 2099 2112 4003
Size: 32 135 32 495 13 19 13 1891
Cuts at: 4003 4035 4077 4109 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1891 495 135 42 32 32 32
32 19 13 13
HincII GTy'rAC
Cuts at: 669 1657 1938 2706 2952 669
Size: 988 281 768 246 3079
Fragments arranged by size:
3079 988 768 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5362
HpaI GTT'AAC
Cuts at: 2952 2952
Size: 5362
KpnI G_GTAC'C
Cuts at: 2687 2687
Size: 5362
MfeI C'AATT_G
Cuts at: 2961 2961
Size: 5362
MluI A'CGCG_T
Cuts at: 5238 5238
Size: 5362
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2525 4476 4660 1305
Size: 1220 1951 184 2007
Fragments arranged by size:
2007 1951 1220 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3540
Fragments arranged by size:
3540 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3320 3679 3838 518
Size: 648 2154 359 159 2042
Fragments arranged by size:
2154 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2162 2278 2778 3455 4603 4848 1606
Size: 556 116 500 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 556 500 245 116
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3871
Fragments arranged by size:
3871 939 552
NdeI CA'TA_TG
Cuts at: 387 2356 2577 387
Size: 1969 221 3172
Fragments arranged by size:
3172 1969 221
NheI G'CTAG_C
Cuts at: 1052 2182 1052
Size: 1130 4232
NotI GC'GGCC_GC
Cuts at: 2809 2809
Size: 5362
NspI r_CATG'y
Cuts at: 1132 1229 2720 4265 1132
Size: 97 1491 1545 2229
Fragments arranged by size:
2229 1545 1491 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5362
PciI A'CATG_T
Cuts at: 4261 4261
Size: 5362
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2281 1108
Size: 1173 4189
PmeI GTTT'AAAC
Cuts at: 2666 2666
Size: 5362
PmlI CAC'GTG
Cuts at: 2295 2295
Size: 5362
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3431 3463 4944 4976 3431
Size: 32 1481 32 3817
Fragments arranged by size:
3817 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5362
PsiI TTA'TAA
Cuts at: 2932 2932
Size: 5362
PstI C_TGCA'G
Cuts at: 830 2254 2714 830
Size: 1424 460 3478
Fragments arranged by size:
3478 1424 460
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5362
PvuII CAG'CTG
Cuts at: 2162 2278 2162
Size: 116 5246
SacI G_AGCT'C
Cuts at: 2538 2538
Size: 5362
SalI G'TCGA_C
Cuts at: 1936 2704 1936
Size: 768 4594
SbfI CC_TGCA'GG
Cuts at: 2714 2714
Size: 5362
ScaI AGT'ACT
Cuts at: 2526 3610 5322 2526
Size: 1084 1712 2566
Fragments arranged by size:
2566 1712 1084
SfcI C'TryA_G
Cuts at: 826 1045 2250 2710 3845 4526 4717 826
Size: 219 1205 460 1135 681 191 1471
Fragments arranged by size:
1471 1205 1135 681 460 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5362
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5362
SmaI CCC'GGG
Cuts at: 1872 2689 1872
Size: 817 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2433 3466 4367 4629 820
Size: 197 926 490 1033 901 262 1553
Fragments arranged by size:
1553 1033 926 901 490 262 197
SnaBI TAC'GTA
Cuts at: 493 3153 493
Size: 2660 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5362
SphI G_CATG'C
Cuts at: 1132 2720 1132
Size: 1588 3774
SspI AAT'ATT
Cuts at: 5 52 3286 5
Size: 47 3234 2081
Fragments arranged by size:
3234 2081 47
StuI AGG'CCT
Cuts at: 1517 2154 2568 1517
Size: 637 414 4311
Fragments arranged by size:
4311 637 414
StyI C'CwwG_G
Cuts at: 513 1065 2004 2801 513
Size: 552 939 797 3074
Fragments arranged by size:
3074 939 797 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3389 3406 3559 3744 4189 325
Size: 1255 1809 17 153 185 445 1498
Fragments arranged by size:
1809 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2418 2524 3608
Size: 274 80 33 51 1443 441 106 1084
Cuts at: 3608 5320 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1084 441 274 138 106 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2238 2270 2279 2311 5322 5354 2238
Size: 32 9 32 3011 32 2246
Fragments arranged by size:
3011 2246 32 32 32 9
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5362
XbaI T'CTAG_A
Cuts at: 2698 2698
Size: 5362
XhoI C'TCGA_G
Cuts at: 1943 2433 1943
Size: 490 4872
XmaI C'CCGG_G
Cuts at: 1870 2687 1870
Size: 817 4545
XmnI GAAnn'nnTTC
Cuts at: 3491 3491
Size: 5362
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3170 276
Size: 53 83 186 936 1636 2468
Fragments arranged by size:
2468 1636 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI