Restriction map for
pFN21AE2215
(Circular) (Six-base) MAPSORT of: pFN21AE2215.seq Check: 8324 from: 1 to: 6061
pFN21AE2215 6061 bp
With 182 enzymes: SgfI *
August 16, 2010 14:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3871 278
Size: 53 83 186 936 2335 2468
Fragments arranged by size:
2468 2335 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2105 3129 3404 1937
Size: 168 1024 275 4594
Fragments arranged by size:
4594 1024 275 168
Acc65I G'GTAC_C
Cuts at: 2727 3382 2727
Size: 655 5406
AclI AA'CG_TT
Cuts at: 1563 4188 4561 1563
Size: 2625 373 3063
Fragments arranged by size:
3063 2625 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2515 4124 5508 5776 1632
Size: 883 1609 1384 268 1917
Fragments arranged by size:
1917 1609 1384 883 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5864
AflIII A'CryG_T
Cuts at: 1203 2814 3267 4960 5937 1203
Size: 1611 453 1693 977 1327
Fragments arranged by size:
1693 1611 1327 977 453
AgeI A'CCGG_T
Cuts at: 2772 2943 4892 2772
Size: 171 1949 3941
Fragments arranged by size:
3941 1949 171
AhdI GACnn_n'nnGTC
Cuts at: 4790 4790
Size: 6061
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6061
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6061
AlwNI CAG_nnn'CTG
Cuts at: 3804 5376 3804
Size: 1572 4489
ApaI G_GGCC'C
Cuts at: 2604 2839 2604
Size: 235 5826
ApaLI G'TGCA_C
Cuts at: 4118 5274 4118
Size: 1156 4905
ApoI r'AATT_y
Cuts at: 1493 2252 3370 3596 3748 5857 1493
Size: 759 1118 226 152 2109 1697
Fragments arranged by size:
2109 1697 1118 759 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4615 160
Size: 872 3583 1606
Fragments arranged by size:
3583 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6061
AvaI C'yCGr_G
Cuts at: 1870 1943 2832 3386 1870
Size: 73 889 554 4545
Fragments arranged by size:
4545 889 554 73
BamHI G'GATC_C
Cuts at: 3391 3758 3391
Size: 367 5694
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2034 2384 2727 3382 4837
Size: 325 217 627 247 350 343 655 1455
Cuts at: 4837 618
Size: 1842
Fragments arranged by size:
1842 1455 655 627 350 343 325 247
217
BanII G_rGCy'C
Cuts at: 1391 2539 2604 2839 3300 3380 1391
Size: 1148 65 235 461 80 4072
Fragments arranged by size:
4072 1148 461 235 80 65
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5090
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2335 2369 4252 4286 1903
Size: 34 398 34 1883 34 3678
Fragments arranged by size:
3678 1883 398 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2503 3953 5163 2503
Size: 1450 1210 3401
Fragments arranged by size:
3401 1450 1210
BclI T'GATC_A
Cuts at: 1548 3171 1548
Size: 1623 4438
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2127 2468
Size: 107 122 71 631 605 337 118 341
Cuts at: 2468 4672 136
Size: 2204 1525
Fragments arranged by size:
2204 1525 631 605 341 337 122 118
107 71
BglII A'GATC_T
Cuts at: 6056 6056
Size: 6061
BlpI GC'TnA_GC
Cuts at: 3478 3478
Size: 6061
Bme1580I G_kGCm'C
Cuts at: 1790 2387 2604 2839 4122 5278 1790
Size: 597 217 235 1283 1156 2573
Fragments arranged by size:
2573 1283 1156 597 235 217
BmgBI CAC'GTC
Cuts at: 1318 2290 1318
Size: 972 5089
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3095 3792 4750
Size: 416 53 83 399 24 1678 697 958
Cuts at: 4750 442
Size: 1753
Fragments arranged by size:
1753 1678 958 697 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6061
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2279 3008 3024 3077 3325 4721 1239
Size: 1040 729 16 53 248 1396 2579
Fragments arranged by size:
2579 1396 1040 729 248 53 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4186 5051 5349 4186
Size: 865 298 4898
Fragments arranged by size:
4898 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3095 3279 4724 882
Size: 617 1596 184 1445 2219
Fragments arranged by size:
2219 1596 1445 617 184
BsaAI yAC'GTr
Cuts at: 493 1204 3268 3852 493
Size: 711 2064 584 2702
Fragments arranged by size:
2702 2064 711 584
BsaBI GATnn'nnATC
Cuts at: 6055 6055
Size: 6061
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3868 4250 4885 275
Size: 53 83 186 936 2335 382 635 1451
Fragments arranged by size:
2335 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2607 2625 2772 2943 4494 4892 5166 5313 5724
Size: 18 147 171 1551 398 274 147 411
Cuts at: 5724 2607
Size: 2944
Fragments arranged by size:
2944 1551 411 398 274 171 147 147
18
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2657 2687 2702 2732 3075
Size: 30 165 30 1254 30 15 30 343
Cuts at: 3075 3105 1178
Size: 30 4134
Fragments arranged by size:
4134 1254 343 165 30 30 30 30
30 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2678 2702 1186
Size: 1492 24 4545
Fragments arranged by size:
4545 1492 24
BseYI C'CCAG_C
Cuts at: 2512 2616 2863 5264 5760 2512
Size: 104 247 2401 496 2813
Fragments arranged by size:
2813 2401 496 247 104
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2693 1163
Size: 573 957 4531
Fragments arranged by size:
4531 957 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2126 2431 2484 3511 4272 5300
Size: 275 186 125 305 53 1027 761 1028
Cuts at: 5300 1540
Size: 2301
Fragments arranged by size:
2301 1028 1027 761 305 275 186 125
53
BsiHKAI G_wGCw'C
Cuts at: 2295 2904 3300 4122 4207 5278 2295
Size: 609 396 822 85 1071 3078
Fragments arranged by size:
3078 1071 822 609 396 85
BsmI GAATG_Cn'
Cuts at: 3572 3665 3572
Size: 93 5968
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2295 2387 2539 2604 2839 2904 3300
Size: 399 505 92 152 65 235 65 396
Cuts at: 3300 3380 4122 4207 5278 1391
Size: 80 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 505 399 396 235 152
92 85 80 65 65
BspEI T'CCGG_A
Cuts at: 2625 5724 2625
Size: 3099 2962
BspHI T'CATG_A
Cuts at: 3950 5680 3950
Size: 1730 4331
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3416 844
Size: 2572 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3948 1394
Size: 429 2125 3507
Fragments arranged by size:
3507 2125 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4556 4730 65
Size: 1183 3308 174 1396
Fragments arranged by size:
3308 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2121 2772 2943 3163 4705 4892 1700
Size: 110 311 651 171 220 1542 187 2869
Fragments arranged by size:
2869 1542 651 311 220 187 171 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6061
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6061
BssSI C'ACGA_G
Cuts at: 4121 5133 4121
Size: 1012 5049
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6061
BstBI TT'CG_AA
Cuts at: 4865 4865
Size: 6061
BstEII G'GTnAC_C
Cuts at: 1184 2851 3236 1184
Size: 1667 385 4009
Fragments arranged by size:
4009 1667 385
BstXI CCAn_nnnn'nTGG
Cuts at: 2986 3085 2986
Size: 99 5962
BstYI r'GATC_y
Cuts at: 1972 2910 3391 3758 4143 4160 5612 5698 5710
Size: 938 481 367 385 17 1452 86 12
Cuts at: 5710 6056 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 938 481 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2045 2098 3138 513
Size: 552 939 41 53 1040 3436
Fragments arranged by size:
3436 1040 939 552 53 41
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5322
Fragments arranged by size:
5322 683 56
BtsI GCAGTG_nn'
Cuts at: 2068 2741 3577 4371 4391 2068
Size: 673 836 794 20 3738
Fragments arranged by size:
3738 836 794 673 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6061
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6026
DraI TTT'AAA
Cuts at: 3365 3365
Size: 6061
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2582 2890 5068 809
Size: 507 1266 308 2178 1802
Fragments arranged by size:
2178 1802 1266 507 308
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2123 2428 2469
Size: 54 1425 50 275 18 293 305 41
Cuts at: 2469 2481 3135 3508 4397 6026 8
Size: 12 654 373 889 1629 43
Fragments arranged by size:
1629 1425 889 654 373 305 293 275
54 50 43 41 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 2123 2428 2481 3508 1537
Size: 275 311 305 53 1027 4090
Fragments arranged by size:
4090 1027 311 305 275 53
EarI CTCTTCn'nnn_
Cuts at: 1353 2624 3991 1353
Size: 1271 1367 3423
Fragments arranged by size:
3423 1367 1271
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2145 4648 5022 5168 387
Size: 1064 138 556 2503 374 146 1280
Fragments arranged by size:
2503 1280 1064 556 374 146 138
EcoICRI GAG'CTC
Cuts at: 3298 3298
Size: 6061
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2279 2515 3008 3024 3077 3325 4124
Size: 393 647 236 493 16 53 248 799
Cuts at: 4124 4721 5508 5776 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 799 787 647 597 493 393 268
248 236 53 16
EcoNI CCTnn'n_nnAGG
Cuts at: 2543 2608 2543
Size: 65 5996
EcoO109I rG'GnC_Cy
Cuts at: 2601 2613 2692 2836 2869 2601
Size: 12 79 144 33 5793
Fragments arranged by size:
5793 144 79 33 12
EcoRI G'AATT_C
Cuts at: 3370 3748 3370
Size: 378 5683
FspI TGC'GCA
Cuts at: 4567 4567
Size: 6061
HaeII r_GCGC'y
Cuts at: 1678 3278 5208 1678
Size: 1600 1930 2531
Fragments arranged by size:
2531 1930 1600
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2477 2509 4702 4734 4776
Size: 32 135 32 905 32 2193 32 42
Cuts at: 4776 4808 1373
Size: 32 2626
Fragments arranged by size:
2626 2193 905 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2106 3130 3405 3651 669
Size: 988 281 168 1024 275 246 3079
Fragments arranged by size:
3079 1024 988 281 275 246 168
HindIII A'AGCT_T
Cuts at: 748 2366 748
Size: 1618 4443
HpaI GTT'AAC
Cuts at: 3651 3651
Size: 6061
KpnI G_GTAC'C
Cuts at: 2731 3386 2731
Size: 655 5406
MfeI C'AATT_G
Cuts at: 3660 3660
Size: 6061
MluI A'CGCG_T
Cuts at: 2814 5937 2814
Size: 3123 2938
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2874 5175 5359 1305
Size: 1569 2301 184 2007
Fragments arranged by size:
2301 2007 1569 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3137 10
Size: 54 1425 343 1305 2934
Fragments arranged by size:
2934 1425 1305 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2050 4019 4378 4537 518
Size: 648 884 1969 359 159 2042
Fragments arranged by size:
2042 1969 884 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2040 2525 3055 3193 3477 4154 5302 5547
Size: 434 485 530 138 284 677 1148 245
Cuts at: 5547 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 530 485 434 284 245
138
NaeI GCC'GGC
Cuts at: 2123 2123
Size: 6061
NcoI C'CATG_G
Cuts at: 513 1065 2004 2098 3138 513
Size: 552 939 94 1040 3436
Fragments arranged by size:
3436 1040 939 552 94
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6061
NgoMIV G'CCGG_C
Cuts at: 2121 2121
Size: 6061
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6061
NotI GC'GGCC_GC
Cuts at: 3508 3508
Size: 6061
NspI r_CATG'y
Cuts at: 1132 1229 3419 4964 1132
Size: 97 2190 1545 2229
Fragments arranged by size:
2229 2190 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2545 2695 1348
Size: 1197 150 4714
Fragments arranged by size:
4714 1197 150
PciI A'CATG_T
Cuts at: 4960 4960
Size: 6061
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2551 1108
Size: 1443 4618
PfoI T'CCnGG_A
Cuts at: 2295 2985 3324 2295
Size: 690 339 5032
Fragments arranged by size:
5032 690 339
PmeI GTTT'AAAC
Cuts at: 3365 3365
Size: 6061
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4130 4162 5643 5675 4130
Size: 32 1481 32 4516
Fragments arranged by size:
4516 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6061
PsiI TTA'TAA
Cuts at: 3631 3631
Size: 6061
PspOMI G'GGCC_C
Cuts at: 2600 2835 2600
Size: 235 5826
PstI C_TGCA'G
Cuts at: 830 3413 830
Size: 2583 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6061
PvuII CAG'CTG
Cuts at: 3055 3193 3055
Size: 138 5923
SacI G_AGCT'C
Cuts at: 3300 3300
Size: 6061
SalI G'TCGA_C
Cuts at: 1936 2104 3128 3403 1936
Size: 168 1024 275 4594
Fragments arranged by size:
4594 1024 275 168
SbfI CC_TGCA'GG
Cuts at: 3413 3413
Size: 6061
ScaI AGT'ACT
Cuts at: 4309 6021 4309
Size: 1712 4349
SfcI C'TryA_G
Cuts at: 826 1045 3409 4544 5225 5416 826
Size: 219 2364 1135 681 191 1471
Fragments arranged by size:
2364 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6061
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6061
SmaI CCC'GGG
Cuts at: 1872 2834 3388 1872
Size: 962 554 4545
Fragments arranged by size:
4545 962 554
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4165 5066 5328 820
Size: 197 926 2222 901 262 1553
Fragments arranged by size:
2222 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3852 493
Size: 3359 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6061
SphI G_CATG'C
Cuts at: 1132 3419 1132
Size: 2287 3774
SspI AAT'ATT
Cuts at: 5 52 3985 5
Size: 47 3933 2081
Fragments arranged by size:
3933 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6061
StyI C'CwwG_G
Cuts at: 513 1065 2004 2098 3078 3138 3500 513
Size: 552 939 94 980 60 362 3074
Fragments arranged by size:
3074 980 939 552 362 94 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4088 4105 4258 4443 4888 325
Size: 1255 2508 17 153 185 445 1498
Fragments arranged by size:
2508 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4307 6019 96
Size: 274 80 33 51 1443 2330 1712 138
Fragments arranged by size:
2330 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2702 2708 2734 2740 3075 3107 6021 6053 2702
Size: 6 26 6 335 32 2914 32 2710
Fragments arranged by size:
2914 2710 335 32 32 26 6 6
Tth111I GACn'n_nGTC
Cuts at: 1316 2582 2849 3125 1316
Size: 1266 267 276 4252
Fragments arranged by size:
4252 1266 276 267
XbaI T'CTAG_A
Cuts at: 3397 3397
Size: 6061
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3223 3223
Size: 6061
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6061
XmaI C'CCGG_G
Cuts at: 1870 2832 3386 1870
Size: 962 554 4545
Fragments arranged by size:
4545 962 554
XmnI GAAnn'nnTTC
Cuts at: 2744 4190 2744
Size: 1446 4615
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3869 276
Size: 53 83 186 936 2335 2468
Fragments arranged by size:
2468 2335 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI