Restriction map for
pFN21AE2213
(Circular) (Six-base) MAPSORT of: pFN21AE2213.seq Check: 2571 from: 1 to: 5824
pFN21AE2213 5824 bp
With 182 enzymes: SgfI *
March 28, 2012 12:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2147 2889 2147
Size: 742 5082
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2306 3634 278
Size: 53 83 186 936 770 1328 2468
Fragments arranged by size:
2468 1328 936 770 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3167 1937
Size: 1230 4594
Acc65I G'GTAC_C
Cuts at: 3145 3145
Size: 5824
AclI AA'CG_TT
Cuts at: 1563 3951 4324 1563
Size: 2388 373 3063
Fragments arranged by size:
3063 2388 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2234 2342 2442 3049 3887 5271 5539 1632
Size: 602 108 100 607 838 1384 268 1917
Fragments arranged by size:
1917 1384 838 607 602 268 108 100
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5627
AflIII A'CryG_T
Cuts at: 1203 4723 5700 1203
Size: 3520 977 1327
Fragments arranged by size:
3520 1327 977
AgeI A'CCGG_T
Cuts at: 2346 4655 2346
Size: 2309 3515
AhdI GACnn_n'nnGTC
Cuts at: 4553 4553
Size: 5824
AleI CACnn'nnGTG
Cuts at: 1166 2551 1166
Size: 1385 4439
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5824
AlwNI CAG_nnn'CTG
Cuts at: 2153 2398 3567 5139 2153
Size: 245 1169 1572 2838
Fragments arranged by size:
2838 1572 1169 245
ApaI G_GGCC'C
Cuts at: 2133 2133
Size: 5824
ApaLI G'TGCA_C
Cuts at: 3881 5037 3881
Size: 1156 4668
ApoI r'AATT_y
Cuts at: 1493 3133 3359 3511 5620 1493
Size: 1640 226 152 2109 1697
Fragments arranged by size:
2109 1697 1640 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4378 160
Size: 872 3346 1606
Fragments arranged by size:
3346 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5824
AvaI C'yCGr_G
Cuts at: 1870 1943 3149 1870
Size: 73 1206 4545
Fragments arranged by size:
4545 1206 73
BamHI G'GATC_C
Cuts at: 2009 3154 3521 2009
Size: 1145 367 4312
Fragments arranged by size:
4312 1145 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3145 4600 618
Size: 325 217 627 1358 1455 1842
Fragments arranged by size:
1842 1455 1358 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2133 2190 2665 3143 1391
Size: 742 57 475 478 4072
Fragments arranged by size:
4072 742 478 475 57
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2958 928
Size: 971 1059 3794
Fragments arranged by size:
3794 1059 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4015 4049 1903
Size: 34 2078 34 3678
Fragments arranged by size:
3678 2078 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3716 4926 3716
Size: 1210 4614
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5824
BfrBI ATG'CAT
Cuts at: 2679 2679
Size: 5824
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4435 136
Size: 107 122 71 631 605 2763 1525
Fragments arranged by size:
2763 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5819 5819
Size: 5824
BlpI GC'TnA_GC
Cuts at: 3241 3241
Size: 5824
Bme1580I G_kGCm'C
Cuts at: 1790 2133 3885 5041 1790
Size: 343 1752 1156 2573
Fragments arranged by size:
2573 1752 1156 343
BmgBI CAC'GTC
Cuts at: 1318 2140 1318
Size: 822 5002
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2866 3555 4513
Size: 416 53 83 399 24 1449 689 958
Cuts at: 4513 442
Size: 1753
Fragments arranged by size:
1753 1449 958 689 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5824
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2420 2515 2679 2906 4484 1239
Size: 1181 95 164 227 1578 2579
Fragments arranged by size:
2579 1578 1181 227 164 95
Bpu10I CC'TnA_GC
Cuts at: 2670 2670
Size: 5824
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3949 4814 5112 3949
Size: 865 298 4661
Fragments arranged by size:
4661 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4487 882
Size: 617 2988 2219
Fragments arranged by size:
2988 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3615 493
Size: 711 2411 2702
Fragments arranged by size:
2702 2411 711
BsaBI GATnn'nnATC
Cuts at: 5818 5818
Size: 5824
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2303 2370 3631 4013
Size: 53 83 186 936 770 67 1261 382
Cuts at: 4013 4648 275
Size: 635 1451
Fragments arranged by size:
1451 1261 936 770 635 382 186 83
67 53
BsaWI w'CCGG_w
Cuts at: 2346 2815 4257 4655 4929 5076 5487 2346
Size: 469 1442 398 274 147 411 2683
Fragments arranged by size:
2683 1442 469 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5599
Fragments arranged by size:
5599 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3038 1186
Size: 1852 3972
BseYI C'CCAG_C
Cuts at: 2095 2352 5027 5523 2095
Size: 257 2675 496 2396
Fragments arranged by size:
2675 2396 496 257
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3023 1163
Size: 573 1287 3964
Fragments arranged by size:
3964 1287 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3274 4035 5063 1540
Size: 275 186 1273 761 1028 2301
Fragments arranged by size:
2301 1273 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2190 2665 2876 3885 3970 5041 2190
Size: 475 211 1009 85 1071 2973
Fragments arranged by size:
2973 1071 1009 475 211 85
BsmI GAATG_Cn'
Cuts at: 2681 2922 3335 3428 2681
Size: 241 413 93 5077
Fragments arranged by size:
5077 413 241 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2225 2396 2225
Size: 171 5653
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2133 2190 2665 2876 3143 3885 3970
Size: 399 343 57 475 211 267 742 85
Cuts at: 3970 5041 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 475 399 343 267 211
85 57
BspEI T'CCGG_A
Cuts at: 2815 5487 2815
Size: 2672 3152
BspHI T'CATG_A
Cuts at: 3713 5443 3713
Size: 1730 4094
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2147 2889 3179 844
Size: 1303 742 290 3489
Fragments arranged by size:
3489 1303 742 290
BsrBI CCG'CTC
Cuts at: 1394 1823 2461 3711 1394
Size: 429 638 1250 3507
Fragments arranged by size:
3507 1250 638 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4319 4493 65
Size: 1183 3071 174 1396
Fragments arranged by size:
3071 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2346 4468 4655 1700
Size: 110 536 2122 187 2869
Fragments arranged by size:
2869 2122 536 187 110
BsrGI T'GTAC_A
Cuts at: 96 2829 96
Size: 2733 3091
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5824
BssSI C'ACGA_G
Cuts at: 3884 4896 3884
Size: 1012 4812
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2153 2482 794
Size: 1359 329 4136
Fragments arranged by size:
4136 1359 329
BstBI TT'CG_AA
Cuts at: 4628 4628
Size: 5824
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5824
BstYI r'GATC_y
Cuts at: 1972 2009 2892 3154 3521 3906 3923 5375 5461
Size: 37 883 262 367 385 17 1452 86
Cuts at: 5461 5473 5819 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 883 385 367 346 262 86
37 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2176 2289 513
Size: 552 939 172 113 4048
Fragments arranged by size:
4048 939 552 172 113
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5085
Fragments arranged by size:
5085 683 56
BtsI GCAGTG_nn'
Cuts at: 2154 3340 4134 4154 2154
Size: 1186 794 20 3824
Fragments arranged by size:
3824 1186 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5824
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2896 2931 529
Size: 35 2332 35 3422
Fragments arranged by size:
3422 2332 35 35
DraI TTT'AAA
Cuts at: 3128 3128
Size: 5824
DraIII CAC_nnn'GTG
Cuts at: 2635 2635
Size: 5824
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4831 809
Size: 507 3515 1802
Fragments arranged by size:
3515 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2436 2838 3271
Size: 54 1425 50 275 18 606 402 433
Cuts at: 3271 4160 5789 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 606 433 402 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3271 1537
Size: 275 1459 4090
Fragments arranged by size:
4090 1459 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2084 2418 2817 3058 3754 1353
Size: 731 334 399 241 696 3423
Fragments arranged by size:
3423 731 696 399 334 241
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4411 4785 4931 387
Size: 1064 138 2822 374 146 1280
Fragments arranged by size:
2822 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2188 2663 2188
Size: 475 5349
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2234 2342 2420 2442 2515 2679 2906
Size: 393 602 108 78 22 73 164 227
Cuts at: 2906 3049 3887 4484 5271 5539 1239
Size: 143 838 597 787 268 1524
Fragments arranged by size:
1524 838 787 602 597 393 268 227
164 143 108 78 73 22
EcoO109I rG'GnC_Cy
Cuts at: 2122 2129 2130 2845 2963 3024 2122
Size: 7 1 715 118 61 4922
Fragments arranged by size:
4922 715 118 61 7 1
EcoRI G'AATT_C
Cuts at: 3133 3511 3133
Size: 378 5446
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2767 2828 2767
Size: 61 5763
FspI TGC'GCA
Cuts at: 4330 4330
Size: 5824
HaeII r_GCGC'y
Cuts at: 1678 2175 4971 1678
Size: 497 2796 2531
Fragments arranged by size:
2796 2531 497
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2688 2720 4465 4497 4539
Size: 32 135 32 1116 32 1745 32 42
Cuts at: 4539 4571 1373
Size: 32 2626
Fragments arranged by size:
2626 1745 1116 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2503 3168 3414 669
Size: 988 281 565 665 246 3079
Fragments arranged by size:
3079 988 665 565 281 246
HindIII A'AGCT_T
Cuts at: 748 2247 748
Size: 1499 4325
HpaI GTT'AAC
Cuts at: 3414 3414
Size: 5824
KpnI G_GTAC'C
Cuts at: 3149 3149
Size: 5824
MfeI C'AATT_G
Cuts at: 3423 3423
Size: 5824
MluI A'CGCG_T
Cuts at: 5700 5700
Size: 5824
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4938 5122 1305
Size: 3633 184 2007
Fragments arranged by size:
3633 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2840 10
Size: 54 1425 343 1008 2994
Fragments arranged by size:
2994 1425 1008 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2204 2551 2999 3782 4141 4300 518
Size: 648 1038 347 448 783 359 159 2042
Fragments arranged by size:
2042 1038 783 648 448 359 347 159
MspA1I CmG'CkG
Cuts at: 1606 2420 3240 3917 5065 5310 1606
Size: 814 820 677 1148 245 2120
Fragments arranged by size:
2120 1148 820 814 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2176 513
Size: 552 939 172 4161
Fragments arranged by size:
4161 939 552 172
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5824
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5824
NotI GC'GGCC_GC
Cuts at: 3271 3271
Size: 5824
NsiI A_TGCA'T
Cuts at: 2681 2681
Size: 5824
NspI r_CATG'y
Cuts at: 1132 1229 2320 2683 2998 3046 3182 4727 1132
Size: 97 1091 363 315 48 136 1545 2229
Fragments arranged by size:
2229 1545 1091 363 315 136 97 48
PasI CC'CwG_GG
Cuts at: 1348 2126 1348
Size: 778 5046
PciI A'CATG_T
Cuts at: 4723 4723
Size: 5824
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2398 2918 1108
Size: 1290 520 4014
Fragments arranged by size:
4014 1290 520
PfoI T'CCnGG_A
Cuts at: 2760 2784 2943 2760
Size: 24 159 5641
Fragments arranged by size:
5641 159 24
PmeI GTTT'AAAC
Cuts at: 3128 3128
Size: 5824
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3893 3925 5406 5438 3893
Size: 32 1481 32 4279
Fragments arranged by size:
4279 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2845 2963 3024 2845
Size: 118 61 5645
Fragments arranged by size:
5645 118 61
PshAI GACnn'nnGTC
Cuts at: 1613 2291 1613
Size: 678 5146
PsiI TTA'TAA
Cuts at: 3394 3394
Size: 5824
PspOMI G'GGCC_C
Cuts at: 2129 2129
Size: 5824
PstI C_TGCA'G
Cuts at: 830 2158 2487 3176 830
Size: 1328 329 689 3478
Fragments arranged by size:
3478 1328 689 329
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5824
SacI G_AGCT'C
Cuts at: 2190 2665 2190
Size: 475 5349
SalI G'TCGA_C
Cuts at: 1936 3166 1936
Size: 1230 4594
SapI GCTCTTCn'nnn_
Cuts at: 2418 2418
Size: 5824
SbfI CC_TGCA'GG
Cuts at: 3176 3176
Size: 5824
ScaI AGT'ACT
Cuts at: 4072 5784 4072
Size: 1712 4112
SfcI C'TryA_G
Cuts at: 826 1045 2154 2443 2465 2483 3172 4307 4988
Size: 219 1109 289 22 18 689 1135 681
Cuts at: 4988 5179 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1109 689 681 289 219 191
22 18
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5824
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5824
SmaI CCC'GGG
Cuts at: 1872 3151 1872
Size: 1279 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3928 4829 5091 820
Size: 197 926 1985 901 262 1553
Fragments arranged by size:
1985 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3615 493
Size: 3122 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5824
SphI G_CATG'C
Cuts at: 1132 2320 3182 1132
Size: 1188 862 3774
Fragments arranged by size:
3774 1188 862
SspI AAT'ATT
Cuts at: 5 52 3748 5
Size: 47 3696 2081
Fragments arranged by size:
3696 2081 47
StuI AGG'CCT
Cuts at: 1517 2027 1517
Size: 510 5314
StyI C'CwwG_G
Cuts at: 513 1065 2004 2176 2560 3263 513
Size: 552 939 172 384 703 3074
Fragments arranged by size:
3074 939 703 552 384 172
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3047 3851 3868 4021 4206 4651 325
Size: 1255 1467 804 17 153 185 445 1498
Fragments arranged by size:
1498 1467 1255 804 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2325 2829 4070
Size: 274 80 33 51 1443 348 504 1241
Cuts at: 4070 5782 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1241 504 348 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5784 5816 5784
Size: 32 5792
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5824
XbaI T'CTAG_A
Cuts at: 3160 3160
Size: 5824
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2059 2730 2059
Size: 671 5153
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5824
XmaI C'CCGG_G
Cuts at: 1870 3149 1870
Size: 1279 4545
XmnI GAAnn'nnTTC
Cuts at: 3953 3953
Size: 5824
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2304 3632 276
Size: 53 83 186 936 770 1328 2468
Fragments arranged by size:
2468 1328 936 770 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI
BsiWI BstXI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI
PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI
SrfI SwaI