Restriction map for
pFN21AE2210
(Circular) (Six-base) MAPSORT of: pFN21AE2210.seq Check: 2106 from: 1 to: 7342
pFN21AE2210 7342 bp
With 182 enzymes: SgfI *
August 16, 2010 14:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2584 2584
Size: 7342
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5152 278
Size: 53 83 186 936 3616 2468
Fragments arranged by size:
3616 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4685 1937
Size: 2748 4594
Acc65I G'GTAC_C
Cuts at: 4663 4663
Size: 7342
AclI AA'CG_TT
Cuts at: 1563 5469 5842 1563
Size: 3906 373 3063
Fragments arranged by size:
3906 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2858 3630 4232 4472 5405 6789 7057 1632
Size: 1226 772 602 240 933 1384 268 1917
Fragments arranged by size:
1917 1384 1226 933 772 602 268 240
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7145
AflIII A'CryG_T
Cuts at: 1203 3450 6241 7218 1203
Size: 2247 2791 977 1327
Fragments arranged by size:
2791 2247 1327 977
AgeI A'CCGG_T
Cuts at: 6173 6173
Size: 7342
AhdI GACnn_n'nnGTC
Cuts at: 6071 6071
Size: 7342
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7342
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3683 1184
Size: 2499 4843
AlwNI CAG_nnn'CTG
Cuts at: 5085 6657 5085
Size: 1572 5770
ApaI G_GGCC'C
Cuts at: 2218 2218
Size: 7342
ApaLI G'TGCA_C
Cuts at: 5399 6555 5399
Size: 1156 6186
ApoI r'AATT_y
Cuts at: 1493 2270 3488 3563 3969 3989 3998 4517 4589
Size: 777 1218 75 406 20 9 519 72
Cuts at: 4589 4651 4877 5029 7138 1493
Size: 62 226 152 2109 1697
Fragments arranged by size:
2109 1697 1218 777 519 406 226 152
75 72 62 20 9
AseI AT'TA_AT
Cuts at: 160 1032 5896 160
Size: 872 4864 1606
Fragments arranged by size:
4864 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7342
AvaI C'yCGr_G
Cuts at: 1870 1943 4667 1870
Size: 73 2724 4545
Fragments arranged by size:
4545 2724 73
BamHI G'GATC_C
Cuts at: 4672 5039 4672
Size: 367 6975
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4663 6118 618
Size: 325 217 627 2876 1455 1842
Fragments arranged by size:
2876 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2218 3531 4661 1391
Size: 827 1313 1130 4072
Fragments arranged by size:
4072 1313 1130 827
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3329 4130 928
Size: 971 1430 801 4140
Fragments arranged by size:
4140 1430 971 801
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5533 5567 1903
Size: 34 3596 34 3678
Fragments arranged by size:
3678 3596 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5234 6444 5234
Size: 1210 6132
BclI T'GATC_A
Cuts at: 1548 3085 1548
Size: 1537 5805
BfrBI ATG'CAT
Cuts at: 2024 2333 2346 3909 2024
Size: 309 13 1563 5457
Fragments arranged by size:
5457 1563 309 13
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5953 136
Size: 107 122 71 631 605 4281 1525
Fragments arranged by size:
4281 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2224 2757 2884 3416 3498 3945 7337 2224
Size: 533 127 532 82 447 3392 2229
Fragments arranged by size:
3392 2229 533 532 447 127 82
BlpI GC'TnA_GC
Cuts at: 2066 4035 4759 2066
Size: 1969 724 4649
Fragments arranged by size:
4649 1969 724
Bme1580I G_kGCm'C
Cuts at: 1790 2218 5403 6559 1790
Size: 428 3185 1156 2573
Fragments arranged by size:
3185 2573 1156 428
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7342
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5073 6031 442
Size: 416 53 83 399 24 3656 958 1753
Fragments arranged by size:
3656 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7342
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3114 3176 3114
Size: 62 7280
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3545 6002 1239
Size: 2306 2457 2579
Fragments arranged by size:
2579 2457 2306
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5467 6332 6630 5467
Size: 865 298 6179
Fragments arranged by size:
6179 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4041 6005 882
Size: 617 2542 1964 2219
Fragments arranged by size:
2542 2219 1964 617
BsaAI yAC'GTr
Cuts at: 493 1204 5133 493
Size: 711 3929 2702
Fragments arranged by size:
3929 2702 711
BsaBI GATnn'nnATC
Cuts at: 7336 7336
Size: 7342
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5149 5531 6166 275
Size: 53 83 186 936 3616 382 635 1451
Fragments arranged by size:
3616 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2373 5775 6173 6447 6594 7005 2373
Size: 3402 398 274 147 411 2710
Fragments arranged by size:
3402 2710 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4074 4104 1178
Size: 30 165 30 2671 30 4416
Fragments arranged by size:
4416 2671 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4095 4358 1186
Size: 2909 263 4170
Fragments arranged by size:
4170 2909 263
BseYI C'CCAG_C
Cuts at: 6545 7041 6545
Size: 496 6846
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2168 2899 3810 1163
Size: 573 432 731 911 4695
Fragments arranged by size:
4695 911 731 573 432
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4792 5553 6581 1540
Size: 275 186 2791 761 1028 2301
Fragments arranged by size:
2791 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5403 5488 6559 5403
Size: 85 1071 6186
Fragments arranged by size:
6186 1071 85
BsmI GAATG_Cn'
Cuts at: 2335 2560 4853 4946 2335
Size: 225 2293 93 4731
Fragments arranged by size:
4731 2293 225 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2218 3531 4661 5403 5488 6559 1391
Size: 399 428 1313 1130 742 85 1071 2174
Fragments arranged by size:
2174 1313 1130 1071 742 428 399 85
BspEI T'CCGG_A
Cuts at: 2373 7005 2373
Size: 4632 2710
BspHI T'CATG_A
Cuts at: 3082 5231 6961 3082
Size: 2149 1730 3463
Fragments arranged by size:
3463 2149 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2140 2584 4697 844
Size: 1296 444 2113 3489
Fragments arranged by size:
3489 2113 1296 444
BsrBI CCG'CTC
Cuts at: 1394 1823 5229 1394
Size: 429 3406 3507
Fragments arranged by size:
3507 3406 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4626 5837 6011 65
Size: 1183 3378 1211 174 1396
Fragments arranged by size:
3378 1396 1211 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5986 6173 1700
Size: 110 4176 187 2869
Fragments arranged by size:
4176 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7342
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7342
BssSI C'ACGA_G
Cuts at: 5402 6414 5402
Size: 1012 6330
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4507 794
Size: 3713 3629
BstBI TT'CG_AA
Cuts at: 6146 6146
Size: 7342
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7342
BstXI CCAn_nnnn'nTGG
Cuts at: 3178 4138 3178
Size: 960 6382
BstYI r'GATC_y
Cuts at: 1972 2224 2757 2884 3365 3416 3498 3945 4571
Size: 252 533 127 481 51 82 447 626
Cuts at: 4571 4672 5039 5424 5441 6893 6979 6991 7337
Size: 101 367 385 17 1452 86 12 346
Cuts at: 7337 1972
Size: 1977
Fragments arranged by size:
1977 1452 626 533 481 447 385 367
346 252 127 101 86 82 51 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6790
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6603
Fragments arranged by size:
6603 683 56
BtsI GCAGTG_nn'
Cuts at: 2316 2948 3665 4858 5652 5672 2316
Size: 632 717 1193 794 20 3986
Fragments arranged by size:
3986 1193 794 717 632 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7342
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7307
DraI TTT'AAA
Cuts at: 4646 4646
Size: 7342
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3048 6349 809
Size: 507 1732 3301 1802
Fragments arranged by size:
3301 1802 1732 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2850 3045 4789
Size: 54 1425 50 275 18 1020 195 1744
Cuts at: 4789 5678 7307 8
Size: 889 1629 43
Fragments arranged by size:
1744 1629 1425 1020 889 275 195 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4789 1537
Size: 275 2977 4090
Fragments arranged by size:
4090 2977 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2964 4470 5272 1353
Size: 1611 1506 802 3423
Fragments arranged by size:
3423 1611 1506 802
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3686 5929 6303 6449 387
Size: 1064 138 2097 2243 374 146 1280
Fragments arranged by size:
2243 2097 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2858 3545 3630 4232 4472 5405 6002
Size: 393 1226 687 85 602 240 933 597
Cuts at: 6002 6789 7057 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1226 933 787 687 602 597 393
268 240 85
EcoO109I rG'GnC_Cy
Cuts at: 2215 2869 3619 2215
Size: 654 750 5938
Fragments arranged by size:
5938 750 654
EcoRI G'AATT_C
Cuts at: 3969 4589 4651 5029 3969
Size: 620 62 378 6282
Fragments arranged by size:
6282 620 378 62
EcoRV GAT'ATC
Cuts at: 2369 2515 2369
Size: 146 7196
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2903 2903
Size: 7342
FspI TGC'GCA
Cuts at: 5848 5848
Size: 7342
HaeII r_GCGC'y
Cuts at: 1678 6489 1678
Size: 4811 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2744 2776 3230 3262 4176
Size: 32 135 32 1172 32 454 32 914
Cuts at: 4176 4208 5983 6015 6057 6089 1373
Size: 32 1775 32 42 32 2626
Fragments arranged by size:
2626 1775 1172 914 454 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2647 4686 4932 669
Size: 988 281 709 2039 246 3079
Fragments arranged by size:
3079 2039 988 709 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7342
HpaI GTT'AAC
Cuts at: 4932 4932
Size: 7342
KpnI G_GTAC'C
Cuts at: 4667 4667
Size: 7342
MfeI C'AATT_G
Cuts at: 4941 4941
Size: 7342
MluI A'CGCG_T
Cuts at: 7218 7218
Size: 7342
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3417 3946 6456 6640 1305
Size: 2112 529 2510 184 2007
Fragments arranged by size:
2510 2112 2007 529 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2852 3047 10
Size: 54 1425 343 1020 195 4305
Fragments arranged by size:
4305 1425 1020 343 195 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2654 3654 4467 5300 5659 5818 518
Size: 648 1488 1000 813 833 359 159 2042
Fragments arranged by size:
2042 1488 1000 833 813 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4758 5435 6583 6828 1606
Size: 3152 677 1148 245 2120
Fragments arranged by size:
3152 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6790
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7342
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7342
NotI GC'GGCC_GC
Cuts at: 4789 4789
Size: 7342
NsiI A_TGCA'T
Cuts at: 2026 2335 2348 3911 2026
Size: 309 13 1563 5457
Fragments arranged by size:
5457 1563 309 13
NspI r_CATG'y
Cuts at: 1132 1229 2024 3454 4700 6245 1132
Size: 97 795 1430 1246 1545 2229
Fragments arranged by size:
2229 1545 1430 1246 795 97
PasI CC'CwG_GG
Cuts at: 1348 4131 1348
Size: 2783 4559
PciI A'CATG_T
Cuts at: 3450 6241 3450
Size: 2791 4551
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7342
PfoI T'CCnGG_A
Cuts at: 4567 4567
Size: 7342
PmeI GTTT'AAAC
Cuts at: 4646 4646
Size: 7342
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2810 2842 5411 5443 6924 6956 2810
Size: 32 2569 32 1481 32 3196
Fragments arranged by size:
3196 2569 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3619 3619
Size: 7342
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7342
PsiI TTA'TAA
Cuts at: 4912 4912
Size: 7342
PspOMI G'GGCC_C
Cuts at: 2214 2214
Size: 7342
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3111 3143 3111
Size: 32 7310
PstI C_TGCA'G
Cuts at: 830 2595 2943 4694 830
Size: 1765 348 1751 3478
Fragments arranged by size:
3478 1765 1751 348
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7342
SalI G'TCGA_C
Cuts at: 1936 4684 1936
Size: 2748 4594
SapI GCTCTTCn'nnn_
Cuts at: 2964 2964
Size: 7342
SbfI CC_TGCA'GG
Cuts at: 4694 4694
Size: 7342
ScaI AGT'ACT
Cuts at: 3859 5590 7302 3859
Size: 1731 1712 3899
Fragments arranged by size:
3899 1731 1712
SfcI C'TryA_G
Cuts at: 826 1045 2591 2939 3575 4690 5825 6506 6697
Size: 219 1546 348 636 1115 1135 681 191
Cuts at: 6697 826
Size: 1471
Fragments arranged by size:
1546 1471 1135 1115 681 636 348 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7342
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7342
SmaI CCC'GGG
Cuts at: 1872 4669 1872
Size: 2797 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5446 6347 6609 820
Size: 197 926 3503 901 262 1553
Fragments arranged by size:
3503 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5133 493
Size: 4640 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7342
SphI G_CATG'C
Cuts at: 1132 2024 4700 1132
Size: 892 2676 3774
Fragments arranged by size:
3774 2676 892
SspI AAT'ATT
Cuts at: 5 52 3717 5266 5
Size: 47 3665 1549 2081
Fragments arranged by size:
3665 2081 1549 47
StuI AGG'CCT
Cuts at: 1517 2912 1517
Size: 1395 5947
StyI C'CwwG_G
Cuts at: 513 1065 4781 513
Size: 552 3716 3074
Fragments arranged by size:
3716 3074 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5369 5386 5539 5724 6169 325
Size: 1255 3789 17 153 185 445 1498
Fragments arranged by size:
3789 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3194 3857 5588
Size: 274 80 33 51 1443 1217 663 1731
Cuts at: 5588 7300 96
Size: 1712 138
Fragments arranged by size:
1731 1712 1443 1217 663 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7302 7334 7302
Size: 32 7310
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7342
XbaI T'CTAG_A
Cuts at: 4201 4432 4678 4201
Size: 231 246 6865
Fragments arranged by size:
6865 246 231
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7342
XmaI C'CCGG_G
Cuts at: 1870 4667 1870
Size: 2797 4545
XmnI GAAnn'nnTTC
Cuts at: 2424 4155 5471 2424
Size: 1731 1316 4295
Fragments arranged by size:
4295 1731 1316
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5150 276
Size: 53 83 186 936 3616 2468
Fragments arranged by size:
3616 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PspXI PvuII RsrII SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI XcmI