Restriction map for
pFN21AE2205
(Circular) (Six-base) MAPSORT of: pFN21AE2205.seq Check: 318 from: 1 to: 6478
pFN21AE2205 6478 bp
With 182 enzymes: SgfI *
August 16, 2010 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4288 278
Size: 53 83 186 936 2752 2468
Fragments arranged by size:
2752 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2493 3821 1937
Size: 556 1328 4594
Fragments arranged by size:
4594 1328 556
Acc65I G'GTAC_C
Cuts at: 3799 3799
Size: 6478
AclI AA'CG_TT
Cuts at: 1563 4605 4978 1563
Size: 3042 373 3063
Fragments arranged by size:
3063 3042 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2108 3215 3347 3644 4541 5925 6193 1632
Size: 476 1107 132 297 897 1384 268 1917
Fragments arranged by size:
1917 1384 1107 897 476 297 268 132
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6281
AflIII A'CryG_T
Cuts at: 1203 3577 5377 6354 1203
Size: 2374 1800 977 1327
Fragments arranged by size:
2374 1800 1327 977
AgeI A'CCGG_T
Cuts at: 5309 5309
Size: 6478
AhdI GACnn_n'nnGTC
Cuts at: 5207 5207
Size: 6478
AleI CACnn'nnGTG
Cuts at: 1166 2381 1166
Size: 1215 5263
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6478
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3119 3151 3119
Size: 32 6446
AlwNI CAG_nnn'CTG
Cuts at: 3055 3316 3325 3500 4221 5793 3055
Size: 261 9 175 721 1572 3740
Fragments arranged by size:
3740 1572 721 261 175 9
ApaI G_GGCC'C
Cuts at: 2033 3157 3619 2033
Size: 1124 462 4892
Fragments arranged by size:
4892 1124 462
ApaLI G'TGCA_C
Cuts at: 4535 5691 4535
Size: 1156 5322
ApoI r'AATT_y
Cuts at: 1493 2801 3193 3787 4013 4165 6274 1493
Size: 1308 392 594 226 152 2109 1697
Fragments arranged by size:
2109 1697 1308 594 392 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5032 160
Size: 872 4000 1606
Fragments arranged by size:
4000 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6478
AvaI C'yCGr_G
Cuts at: 1870 1943 3803 1870
Size: 73 1860 4545
Fragments arranged by size:
4545 1860 73
BamHI G'GATC_C
Cuts at: 3808 4175 3808
Size: 367 6111
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3346 3799 5254 618
Size: 325 217 627 1559 453 1455 1842
Fragments arranged by size:
1842 1559 1455 627 453 325 217
BanII G_rGCy'C
Cuts at: 1391 2033 3157 3619 3797 1391
Size: 642 1124 462 178 4072
Fragments arranged by size:
4072 1124 642 462 178
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2701 3091 3647 928
Size: 971 802 390 556 3759
Fragments arranged by size:
3759 971 802 556 390
BbvCI CC'TCA_GC
Cuts at: 2819 2819
Size: 6478
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2362 2396 4669 4703 1903
Size: 34 425 34 2273 34 3678
Fragments arranged by size:
3678 2273 425 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3354 4370 5580 3354
Size: 1016 1210 4252
Fragments arranged by size:
4252 1210 1016
BclI T'GATC_A
Cuts at: 1548 3433 1548
Size: 1885 4593
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5089 136
Size: 107 122 71 631 605 3417 1525
Fragments arranged by size:
3417 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6473 6473
Size: 6478
BlpI GC'TnA_GC
Cuts at: 3895 3895
Size: 6478
Bme1580I G_kGCm'C
Cuts at: 1790 2033 3157 3619 4539 5695 1790
Size: 243 1124 462 920 1156 2573
Fragments arranged by size:
2573 1156 1124 920 462 243
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6478
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3151 3613 4209
Size: 416 53 83 399 24 1734 462 596
Cuts at: 4209 5167 442
Size: 958 1753
Fragments arranged by size:
1753 1734 958 596 462 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6478
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2356 2356
Size: 6478
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1995 3032 5138 1239
Size: 756 1037 2106 2579
Fragments arranged by size:
2579 2106 1037 756
Bpu10I CC'TnA_GC
Cuts at: 2369 2819 2369
Size: 450 6028
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3665 4603 5468 5766 3665
Size: 938 865 298 4377
Fragments arranged by size:
4377 938 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5141 882
Size: 617 3642 2219
Fragments arranged by size:
3642 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4269 493
Size: 711 3065 2702
Fragments arranged by size:
3065 2702 711
BsaBI GATnn'nnATC
Cuts at: 6472 6472
Size: 6478
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4285 4667 5302 275
Size: 53 83 186 936 2752 382 635 1451
Fragments arranged by size:
2752 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4911 5309 5583 5730 6141 4911
Size: 398 274 147 411 5248
Fragments arranged by size:
5248 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6253
Fragments arranged by size:
6253 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2461 3554 1186
Size: 1275 1093 4110
Fragments arranged by size:
4110 1275 1093
BseYI C'CCAG_C
Cuts at: 2180 5681 6177 2180
Size: 3501 496 2481
Fragments arranged by size:
3501 2481 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3047 3050 1163
Size: 573 1311 3 4591
Fragments arranged by size:
4591 1311 573 3
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3928 4689 5717 1540
Size: 275 186 1927 761 1028 2301
Fragments arranged by size:
2301 1927 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4539 4624 5695 4539
Size: 85 1071 5322
Fragments arranged by size:
5322 1071 85
BsmI GAATG_Cn'
Cuts at: 2853 3989 4082 2853
Size: 1136 93 5249
Fragments arranged by size:
5249 1136 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3305 3305
Size: 6478
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2033 3157 3619 3797 4539 4624 5695
Size: 399 243 1124 462 178 742 85 1071
Cuts at: 5695 1391
Size: 2174
Fragments arranged by size:
2174 1124 1071 742 462 399 243 178
85
BspEI T'CCGG_A
Cuts at: 6141 6141
Size: 6478
BspHI T'CATG_A
Cuts at: 3102 4367 6097 3102
Size: 1265 1730 3483
Fragments arranged by size:
3483 1730 1265
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3072 3833 844
Size: 2228 761 3489
Fragments arranged by size:
3489 2228 761
BsrBI CCG'CTC
Cuts at: 1394 1823 2723 4365 1394
Size: 429 900 1642 3507
Fragments arranged by size:
3507 1642 900 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4973 5147 65
Size: 1183 3725 174 1396
Fragments arranged by size:
3725 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5122 5309 1700
Size: 110 3312 187 2869
Fragments arranged by size:
3312 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2385 96
Size: 2289 4189
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6478
BssSI C'ACGA_G
Cuts at: 4538 5550 4538
Size: 1012 5466
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3500 794
Size: 2706 3772
BstBI TT'CG_AA
Cuts at: 5282 5282
Size: 6478
BstEII G'GTnAC_C
Cuts at: 1184 3293 1184
Size: 2109 4369
BstYI r'GATC_y
Cuts at: 1972 2091 2346 2920 3808 4175 4560 4577 6029
Size: 119 255 574 888 367 385 17 1452
Cuts at: 6029 6115 6127 6473 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 888 574 385 367 346 255
119 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2418 3036 513
Size: 552 939 414 618 3955
Fragments arranged by size:
3955 939 618 552 414
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5739
Fragments arranged by size:
5739 683 56
BtsI GCAGTG_nn'
Cuts at: 2586 2920 2985 3994 4788 4808 2586
Size: 334 65 1009 794 20 4256
Fragments arranged by size:
4256 1009 794 334 65 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6478
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6443
DraI TTT'AAA
Cuts at: 3782 3782
Size: 6478
DraIII CAC_nnn'GTG
Cuts at: 2437 2437
Size: 6478
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3436 5485 809
Size: 507 2120 2049 1802
Fragments arranged by size:
2120 2049 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2358 3925 4814
Size: 54 1425 50 275 18 528 1567 889
Cuts at: 4814 6443 8
Size: 1629 43
Fragments arranged by size:
1629 1567 1425 889 528 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3925 1537
Size: 275 2113 4090
Fragments arranged by size:
4090 2113 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2760 3557 4408 1353
Size: 1407 797 851 3423
Fragments arranged by size:
3423 1407 851 797
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5065 5439 5585 387
Size: 1064 138 3476 374 146 1280
Fragments arranged by size:
3476 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1995 2108 3032 3215 3347 3644 4541
Size: 393 363 113 924 183 132 297 897
Cuts at: 4541 5138 5925 6193 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 924 897 787 597 393 363 297
268 183 132 113
EcoNI CCTnn'n_nnAGG
Cuts at: 3680 3680
Size: 6478
EcoO109I rG'GnC_Cy
Cuts at: 2302 3153 3615 2302
Size: 851 462 5165
Fragments arranged by size:
5165 851 462
EcoRI G'AATT_C
Cuts at: 3193 3787 4165 3193
Size: 594 378 5506
Fragments arranged by size:
5506 594 378
EcoRV GAT'ATC
Cuts at: 3733 3733
Size: 6478
FspI TGC'GCA
Cuts at: 4984 4984
Size: 6478
HaeII r_GCGC'y
Cuts at: 1678 5625 1678
Size: 3947 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2568 2600 3634 3666 5119
Size: 32 135 32 996 32 1034 32 1453
Cuts at: 5119 5151 5193 5225 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1453 1034 996 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3822 4068 669
Size: 988 281 1884 246 3079
Fragments arranged by size:
3079 1884 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2650 748
Size: 1902 4576
HpaI GTT'AAC
Cuts at: 4068 4068
Size: 6478
KpnI G_GTAC'C
Cuts at: 3803 3803
Size: 6478
MfeI C'AATT_G
Cuts at: 3587 4077 3587
Size: 490 5988
MluI A'CGCG_T
Cuts at: 6354 6354
Size: 6478
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3141 3495 5592 5776 1305
Size: 1836 354 2097 184 2007
Fragments arranged by size:
2097 2007 1836 354 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2360 10
Size: 54 1425 343 528 4128
Fragments arranged by size:
4128 1425 528 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2381 2533 2829 4436 4795 4954 518
Size: 648 1215 152 296 1607 359 159 2042
Fragments arranged by size:
2042 1607 1215 648 359 296 159 152
MspA1I CmG'CkG
Cuts at: 1606 2065 2504 3031 3894 4571 5719 5964 1606
Size: 459 439 527 863 677 1148 245 2120
Fragments arranged by size:
2120 1148 863 677 527 459 439 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2418 513
Size: 552 939 414 4573
Fragments arranged by size:
4573 939 552 414
NdeI CA'TA_TG
Cuts at: 387 3725 387
Size: 3338 3140
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6478
NotI GC'GGCC_GC
Cuts at: 3925 3925
Size: 6478
NspI r_CATG'y
Cuts at: 1132 1229 3581 3836 5381 1132
Size: 97 2352 255 1545 2229
Fragments arranged by size:
2352 2229 1545 255 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6478
PciI A'CATG_T
Cuts at: 3577 5377 3577
Size: 1800 4678
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2864 3625 1108
Size: 1756 761 3961
Fragments arranged by size:
3961 1756 761
PfoI T'CCnGG_A
Cuts at: 2342 3048 2342
Size: 706 5772
PmeI GTTT'AAAC
Cuts at: 3782 3782
Size: 6478
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3663 3695 4547 4579 6060 6092 3663
Size: 32 852 32 1481 32 4049
Fragments arranged by size:
4049 1481 852 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2897 1613
Size: 1284 5194
PsiI TTA'TAA
Cuts at: 4048 4048
Size: 6478
PspOMI G'GGCC_C
Cuts at: 2029 3153 3615 2029
Size: 1124 462 4892
Fragments arranged by size:
4892 1124 462
PstI C_TGCA'G
Cuts at: 830 3321 3496 3830 830
Size: 2491 175 334 3478
Fragments arranged by size:
3478 2491 334 175
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6478
PvuII CAG'CTG
Cuts at: 2065 2504 3031 2065
Size: 439 527 5512
Fragments arranged by size:
5512 527 439
SalI G'TCGA_C
Cuts at: 1936 3820 1936
Size: 1884 4594
SapI GCTCTTCn'nnn_
Cuts at: 2760 2760
Size: 6478
SbfI CC_TGCA'GG
Cuts at: 3830 3830
Size: 6478
ScaI AGT'ACT
Cuts at: 3338 4726 6438 3338
Size: 1388 1712 3378
Fragments arranged by size:
3378 1712 1388
SexAI A'CCwGG_T
Cuts at: 2476 3342 2476
Size: 866 5612
SfcI C'TryA_G
Cuts at: 826 1045 2490 3317 3425 3492 3826 4961 5642
Size: 219 1445 827 108 67 334 1135 681
Cuts at: 5642 5833 826
Size: 191 1471
Fragments arranged by size:
1471 1445 1135 827 681 334 219 191
108 67
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6478
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6478
SmaI CCC'GGG
Cuts at: 1872 3805 1872
Size: 1933 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3680 4582 5483 5745 820
Size: 197 926 1737 902 901 262 1553
Fragments arranged by size:
1737 1553 926 902 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4269 493
Size: 3776 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6478
SphI G_CATG'C
Cuts at: 1132 3836 1132
Size: 2704 3774
SspI AAT'ATT
Cuts at: 5 52 3389 4402 5
Size: 47 3337 1013 2081
Fragments arranged by size:
3337 2081 1013 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6478
StyI C'CwwG_G
Cuts at: 513 1065 2004 2229 2355 2418 2952 3350 3399
Size: 552 939 225 126 63 534 398 49
Cuts at: 3399 3917 513
Size: 518 3074
Fragments arranged by size:
3074 939 552 534 518 398 225 126
63 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4505 4522 4675 4860 5305 325
Size: 1255 2925 17 153 185 445 1498
Fragments arranged by size:
2925 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2385 3336 4724
Size: 274 80 33 51 1443 408 951 1388
Cuts at: 4724 6436 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1388 951 408 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2233 2265 6438 6470 2233
Size: 32 4173 32 2241
Fragments arranged by size:
4173 2241 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6478
XbaI T'CTAG_A
Cuts at: 2875 3814 2875
Size: 939 5539
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2532 3406 2532
Size: 874 5604
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6478
XmaI C'CCGG_G
Cuts at: 1870 3803 1870
Size: 1933 4545
XmnI GAAnn'nnTTC
Cuts at: 2702 3113 3277 3675 4607 2702
Size: 411 164 398 932 4573
Fragments arranged by size:
4573 932 411 398 164
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4286 276
Size: 53 83 186 936 2752 2468
Fragments arranged by size:
2752 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BfrBI BsiWI
BstXI BstZ17I Bsu36I EcoICRI FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI
SrfI SwaI