Restriction map for
pFN21AE2203
(Circular) (Six-base) MAPSORT of: pFN21AE2203.seq Check: 9758 from: 1 to: 7330
pFN21AE2203 7330 bp
With 182 enzymes: SgfI *
July 6, 2012 17:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5140 278
Size: 53 83 186 936 3604 2468
Fragments arranged by size:
3604 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3046 4673 1937
Size: 1109 1627 4594
Fragments arranged by size:
4594 1627 1109
Acc65I G'GTAC_C
Cuts at: 4651 4651
Size: 7330
AclI AA'CG_TT
Cuts at: 1563 5457 5830 1563
Size: 3894 373 3063
Fragments arranged by size:
3894 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2147 2960 3152 3281 3527 4175 4437 5393
Size: 515 813 192 129 246 648 262 956
Cuts at: 5393 6777 7045 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 956 813 648 515 268 262
246 192 129
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7133
AflIII A'CryG_T
Cuts at: 1203 3529 6229 7206 1203
Size: 2326 2700 977 1327
Fragments arranged by size:
2700 2326 1327 977
AgeI A'CCGG_T
Cuts at: 6161 6161
Size: 7330
AhdI GACnn_n'nnGTC
Cuts at: 6059 6059
Size: 7330
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7330
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4137 1184
Size: 2953 4377
AlwNI CAG_nnn'CTG
Cuts at: 2989 5073 6645 2989
Size: 2084 1572 3674
Fragments arranged by size:
3674 2084 1572
ApaLI G'TGCA_C
Cuts at: 2590 5387 6543 2590
Size: 2797 1156 3377
Fragments arranged by size:
3377 2797 1156
ApoI r'AATT_y
Cuts at: 1493 3644 4045 4205 4639 4865 5017 7126 1493
Size: 2151 401 160 434 226 152 2109 1697
Fragments arranged by size:
2151 2109 1697 434 401 226 160 152
AseI AT'TA_AT
Cuts at: 160 1032 5884 160
Size: 872 4852 1606
Fragments arranged by size:
4852 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7330
AvaI C'yCGr_G
Cuts at: 1870 1943 4655 1870
Size: 73 2712 4545
Fragments arranged by size:
4545 2712 73
BamHI G'GATC_C
Cuts at: 4660 5027 4660
Size: 367 6963
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3523 4651 6106 618
Size: 325 217 627 1736 1128 1455 1842
Fragments arranged by size:
1842 1736 1455 1128 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2041 3409 3616 4649 1391
Size: 650 1368 207 1033 4072
Fragments arranged by size:
4072 1368 1033 650 207
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3768 4512 928
Size: 971 1869 744 3746
Fragments arranged by size:
3746 1869 971 744
BbvCI CC'TCA_GC
Cuts at: 4137 4137
Size: 7330
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2985 3019 5521 5555 1903
Size: 34 1048 34 2502 34 3678
Fragments arranged by size:
3678 2502 1048 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4561 5222 6432 4561
Size: 661 1210 5459
Fragments arranged by size:
5459 1210 661
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7330
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5941 136
Size: 107 122 71 631 605 4269 1525
Fragments arranged by size:
4269 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7325 7325
Size: 7330
BlpI GC'TnA_GC
Cuts at: 4747 4747
Size: 7330
Bme1580I G_kGCm'C
Cuts at: 1790 2594 5391 6547 1790
Size: 804 2797 1156 2573
Fragments arranged by size:
2797 2573 1156 804
BmgBI CAC'GTC
Cuts at: 1318 4510 1318
Size: 3192 4138
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5061 6019 442
Size: 416 53 83 399 24 3644 958 1753
Fragments arranged by size:
3644 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7330
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5990 1239
Size: 4751 2579
Bpu10I CC'TnA_GC
Cuts at: 4137 4137
Size: 7330
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2821 3366 3795 5455 6320 6618 2821
Size: 545 429 1660 865 298 3533
Fragments arranged by size:
3533 1660 865 545 429 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2528 5993 882
Size: 617 1029 3465 2219
Fragments arranged by size:
3465 2219 1029 617
BsaAI yAC'GTr
Cuts at: 493 1204 5121 493
Size: 711 3917 2702
Fragments arranged by size:
3917 2702 711
BsaBI GATnn'nnATC
Cuts at: 7324 7324
Size: 7330
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5137 5519 6154 275
Size: 53 83 186 936 3604 382 635 1451
Fragments arranged by size:
3604 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5763 6161 6435 6582 6993 5763
Size: 398 274 147 411 6100
Fragments arranged by size:
6100 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4480 4510 1178
Size: 30 165 30 3077 30 3998
Fragments arranged by size:
3998 3077 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3902 4501 1186
Size: 2716 599 4015
Fragments arranged by size:
4015 2716 599
BseYI C'CCAG_C
Cuts at: 2428 6533 7029 2428
Size: 4105 496 2729
Fragments arranged by size:
4105 2729 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3305 1163
Size: 573 1569 5188
Fragments arranged by size:
5188 1569 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3153 4780 5541 6569 1540
Size: 275 186 1152 1627 761 1028 2301
Fragments arranged by size:
2301 1627 1152 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2594 3409 3717 5391 5476 6547 2594
Size: 815 308 1674 85 1071 3377
Fragments arranged by size:
3377 1674 1071 815 308 85
BsmI GAATG_Cn'
Cuts at: 2719 4841 4934 2719
Size: 2122 93 5115
Fragments arranged by size:
5115 2122 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3427 3427
Size: 7330
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2041 2594 3409 3616 3717 4649 5391
Size: 399 251 553 815 207 101 932 742
Cuts at: 5391 5476 6547 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 932 815 742 553 399 251
207 101 85
BspEI T'CCGG_A
Cuts at: 6993 6993
Size: 7330
BspHI T'CATG_A
Cuts at: 2506 2833 5219 6949 2506
Size: 327 2386 1730 2887
Fragments arranged by size:
2887 2386 1730 327
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2134 3203 4685 844
Size: 1290 1069 1482 3489
Fragments arranged by size:
3489 1482 1290 1069
BsrBI CCG'CTC
Cuts at: 1394 1823 2339 5217 1394
Size: 429 516 2878 3507
Fragments arranged by size:
3507 2878 516 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2414 5825 5999 65
Size: 1183 1166 3411 174 1396
Fragments arranged by size:
3411 1396 1183 1166 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3006 4571 5974 6161 1700
Size: 110 1196 1565 1403 187 2869
Fragments arranged by size:
2869 1565 1403 1196 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7330
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7330
BssSI C'ACGA_G
Cuts at: 2585 5390 6402 2585
Size: 2805 1012 3513
Fragments arranged by size:
3513 2805 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7330
BstBI TT'CG_AA
Cuts at: 6134 6134
Size: 7330
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7330
BstXI CCAn_nnnn'nTGG
Cuts at: 2308 3139 3574 2308
Size: 831 435 6064
Fragments arranged by size:
6064 831 435
BstYI r'GATC_y
Cuts at: 1972 2351 2542 3806 4393 4660 5027 5412 5429
Size: 379 191 1264 587 267 367 385 17
Cuts at: 5429 6881 6967 6979 7325 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1264 587 385 379 367 346
267 191 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3183 3183
Size: 7330
BtgI C'CryG_G
Cuts at: 513 1065 2004 3040 513
Size: 552 939 1036 4803
Fragments arranged by size:
4803 1036 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6591
Fragments arranged by size:
6591 683 56
BtsI GCAGTG_nn'
Cuts at: 4106 4846 5640 5660 4106
Size: 740 794 20 5776
Fragments arranged by size:
5776 794 740 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7330
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7295
DraI TTT'AAA
Cuts at: 3396 4051 4634 3396
Size: 655 583 6092
Fragments arranged by size:
6092 655 583
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4521 6337 809
Size: 507 3205 1816 1802
Fragments arranged by size:
3205 1816 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2016 2340 3150
Size: 54 1425 50 275 18 186 324 810
Cuts at: 3150 4103 4777 5666 7295 8
Size: 953 674 889 1629 43
Fragments arranged by size:
1629 1425 953 889 810 674 324 275
186 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3150 4777 1537
Size: 275 1338 1627 4090
Fragments arranged by size:
4090 1627 1338 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2942 3777 3828 4034 4191 4276 4314 4478
Size: 1589 835 51 206 157 85 38 164
Cuts at: 4478 4590 5260 1353
Size: 112 670 3423
Fragments arranged by size:
3423 1589 835 670 206 164 157 112
85 51 38
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5917 6291 6437 387
Size: 1064 138 4328 374 146 1280
Fragments arranged by size:
4328 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3407 3407
Size: 7330
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2147 2960 3152 3281 3527 4175 4437
Size: 393 515 813 192 129 246 648 262
Cuts at: 4437 5393 5990 6777 7045 1239
Size: 956 597 787 268 1524
Fragments arranged by size:
1524 956 813 787 648 597 515 393
268 262 246 192 129
EcoO109I rG'GnC_Cy
Cuts at: 2788 2788
Size: 7330
EcoRI G'AATT_C
Cuts at: 4639 5017 4639
Size: 378 6952
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2348 2348
Size: 7330
FspI TGC'GCA
Cuts at: 5836 5836
Size: 7330
HaeII r_GCGC'y
Cuts at: 1678 6477 1678
Size: 4799 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2050 2082 2920 2952 3656
Size: 32 135 32 478 32 838 32 704
Cuts at: 3656 3688 5971 6003 6045 6077 1373
Size: 32 2283 32 42 32 2626
Fragments arranged by size:
2626 2283 838 704 478 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3047 4674 4920 669
Size: 988 281 1109 1627 246 3079
Fragments arranged by size:
3079 1627 1109 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3246 748
Size: 2498 4832
HpaI GTT'AAC
Cuts at: 4920 4920
Size: 7330
KpnI G_GTAC'C
Cuts at: 4655 4655
Size: 7330
MfeI C'AATT_G
Cuts at: 4929 4929
Size: 7330
MluI A'CGCG_T
Cuts at: 3529 7206 3529
Size: 3677 3653
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6444 6628 1305
Size: 5139 184 2007
Fragments arranged by size:
5139 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4105 10
Size: 54 1425 343 2273 3235
Fragments arranged by size:
3235 2273 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2619 5288 5647 5806 518
Size: 648 1453 2669 359 159 2042
Fragments arranged by size:
2669 2042 1453 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2519 4746 5423 6571 6816 1606
Size: 913 2227 677 1148 245 2120
Fragments arranged by size:
2227 2120 1148 913 677 245
NaeI GCC'GGC
Cuts at: 3008 3008
Size: 7330
NcoI C'CATG_G
Cuts at: 513 1065 2004 3040 513
Size: 552 939 1036 4803
Fragments arranged by size:
4803 1036 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7330
NgoMIV G'CCGG_C
Cuts at: 3006 3006
Size: 7330
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7330
NotI GC'GGCC_GC
Cuts at: 4777 4777
Size: 7330
NspI r_CATG'y
Cuts at: 1132 1229 4688 6233 1132
Size: 97 3459 1545 2229
Fragments arranged by size:
3459 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7330
PciI A'CATG_T
Cuts at: 6229 6229
Size: 7330
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7330
PmeI GTTT'AAAC
Cuts at: 4634 4634
Size: 7330
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5399 5431 6912 6944 5399
Size: 32 1481 32 5785
Fragments arranged by size:
5785 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2788 2788
Size: 7330
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7330
PsiI TTA'TAA
Cuts at: 4900 4900
Size: 7330
PstI C_TGCA'G
Cuts at: 830 2898 3106 4682 830
Size: 2068 208 1576 3478
Fragments arranged by size:
3478 2068 1576 208
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7330
PvuII CAG'CTG
Cuts at: 2519 2519
Size: 7330
SacI G_AGCT'C
Cuts at: 3409 3409
Size: 7330
SalI G'TCGA_C
Cuts at: 1936 3045 4672 1936
Size: 1109 1627 4594
Fragments arranged by size:
4594 1627 1109
SapI GCTCTTCn'nnn_
Cuts at: 2942 4590 2942
Size: 1648 5682
SbfI CC_TGCA'GG
Cuts at: 4682 4682
Size: 7330
ScaI AGT'ACT
Cuts at: 3108 5578 7290 3108
Size: 2470 1712 3148
Fragments arranged by size:
3148 2470 1712
SexAI A'CCwGG_T
Cuts at: 2596 2596
Size: 7330
SfcI C'TryA_G
Cuts at: 826 1045 2894 3102 4678 5813 6494 6685 826
Size: 219 1849 208 1576 1135 681 191 1471
Fragments arranged by size:
1849 1576 1471 1135 681 219 208 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7330
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7330
SmaI CCC'GGG
Cuts at: 1872 4657 1872
Size: 2785 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2800 3381 3810 5434 6335 6597
Size: 197 926 857 581 429 1624 901 262
Cuts at: 6597 820
Size: 1553
Fragments arranged by size:
1624 1553 926 901 857 581 429 262
197
SnaBI TAC'GTA
Cuts at: 493 5121 493
Size: 4628 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7330
SphI G_CATG'C
Cuts at: 1132 4688 1132
Size: 3556 3774
SspI AAT'ATT
Cuts at: 5 52 5254 5
Size: 47 5202 2081
Fragments arranged by size:
5202 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7330
StyI C'CwwG_G
Cuts at: 513 1065 2004 3040 3608 4769 513
Size: 552 939 1036 568 1161 3074
Fragments arranged by size:
3074 1161 1036 939 568 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5357 5374 5527 5712 6157 325
Size: 1255 3777 17 153 185 445 1498
Fragments arranged by size:
3777 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2329 3106 5576
Size: 274 80 33 51 1443 352 777 2470
Cuts at: 5576 7288 96
Size: 1712 138
Fragments arranged by size:
2470 1712 1443 777 352 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4480 4512 7290 7322 4480
Size: 32 2778 32 4488
Fragments arranged by size:
4488 2778 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7330
XbaI T'CTAG_A
Cuts at: 4666 4666
Size: 7330
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3571 3634 3571
Size: 63 7267
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7330
XmaI C'CCGG_G
Cuts at: 1870 4655 1870
Size: 2785 4545
XmnI GAAnn'nnTTC
Cuts at: 4188 4329 5459 4188
Size: 141 1130 6059
Fragments arranged by size:
6059 1130 141
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5138 276
Size: 53 83 186 936 3604 2468
Fragments arranged by size:
3604 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BstZ17I DraIII EcoNI EcoRV FseI
FspAI KasI NarI NruI NsiI PacI PfoI PmlI
PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI
SrfI SwaI