Restriction map for
pFN21AE2202
(Circular) (Six-base) MAPSORT of: pFN21AE2202.seq Check: 1734 from: 1 to: 7534
pFN21AE2202 7534 bp
With 182 enzymes: SgfI *
September 21, 2016 11:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5344 278
Size: 53 83 186 936 3808 2468
Fragments arranged by size:
3808 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2059 4450 4578 4877 1937
Size: 122 2391 128 299 4594
Fragments arranged by size:
4594 2391 299 128 122
Acc65I G'GTAC_C
Cuts at: 4855 4855
Size: 7534
AclI AA'CG_TT
Cuts at: 1563 5661 6034 1563
Size: 4098 373 3063
Fragments arranged by size:
4098 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3315 3555 3621 3816 4023 4746 5597 6981
Size: 1683 240 66 195 207 723 851 1384
Cuts at: 6981 7249 1632
Size: 268 1917
Fragments arranged by size:
1917 1683 1384 851 723 268 240 207
195 66
AfeI AGC'GCT
Cuts at: 3500 3500
Size: 7534
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7337
AflIII A'CryG_T
Cuts at: 1203 3989 4232 6433 7410 1203
Size: 2786 243 2201 977 1327
Fragments arranged by size:
2786 2201 1327 977 243
AgeI A'CCGG_T
Cuts at: 6365 6365
Size: 7534
AhdI GACnn_n'nnGTC
Cuts at: 6263 6263
Size: 7534
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7534
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7534
AlwNI CAG_nnn'CTG
Cuts at: 5277 6849 5277
Size: 1572 5962
ApaLI G'TGCA_C
Cuts at: 5591 6747 5591
Size: 1156 6378
ApoI r'AATT_y
Cuts at: 1493 2164 2361 2456 2566 2763 2909 3275 3362
Size: 671 197 95 110 197 146 366 87
Cuts at: 3362 3373 3570 3828 4843 5069 5221 7330 1493
Size: 11 197 258 1015 226 152 2109 1697
Fragments arranged by size:
2109 1697 1015 671 366 258 226 197
197 197 152 146 110 95 87 11
AseI AT'TA_AT
Cuts at: 160 1032 6088 160
Size: 872 5056 1606
Fragments arranged by size:
5056 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7534
AvaI C'yCGr_G
Cuts at: 1870 1943 4859 1870
Size: 73 2916 4545
Fragments arranged by size:
4545 2916 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2493 2526 2493
Size: 33 7501
BamHI G'GATC_C
Cuts at: 4864 5231 4864
Size: 367 7167
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4855 6310 618
Size: 325 217 627 3068 1455 1842
Fragments arranged by size:
3068 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4853 1391
Size: 3462 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3755 928
Size: 971 1856 4707
Fragments arranged by size:
4707 1856 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5725 5759 1903
Size: 34 3788 34 3678
Fragments arranged by size:
3788 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3142 4585 5426 6636 3142
Size: 1443 841 1210 4040
Fragments arranged by size:
4040 1443 1210 841
BclI T'GATC_A
Cuts at: 1548 2431 1548
Size: 883 6651
BfrBI ATG'CAT
Cuts at: 3661 3661
Size: 7534
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6145 136
Size: 107 122 71 631 605 4473 1525
Fragments arranged by size:
4473 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2948 3191 3908 7529 2948
Size: 243 717 3621 2953
Fragments arranged by size:
3621 2953 717 243
BlpI GC'TnA_GC
Cuts at: 4951 4951
Size: 7534
Bme1580I G_kGCm'C
Cuts at: 1790 2074 2539 5595 6751 1790
Size: 284 465 3056 1156 2573
Fragments arranged by size:
3056 2573 1156 465 284
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7534
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3104 4620 4749
Size: 416 53 83 399 24 1687 1516 129
Cuts at: 4749 5265 6223 442
Size: 516 958 1753
Fragments arranged by size:
1753 1687 1516 958 516 416 399 129
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7534
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3825 4044 4308 6194 1239
Size: 2586 219 264 1886 2579
Fragments arranged by size:
2586 2579 1886 264 219
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5659 6524 6822 5659
Size: 865 298 6371
Fragments arranged by size:
6371 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2985 6197 882
Size: 617 1486 3212 2219
Fragments arranged by size:
3212 2219 1486 617
BsaAI yAC'GTr
Cuts at: 493 1204 2075 5325 493
Size: 711 871 3250 2702
Fragments arranged by size:
3250 2702 871 711
BsaBI GATnn'nnATC
Cuts at: 4542 7528 4542
Size: 2986 4548
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3580 5341 5723 6358
Size: 53 83 186 936 2047 1761 382 635
Cuts at: 6358 275
Size: 1451
Fragments arranged by size:
2047 1761 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2015 4791 5967 6365 6639 6786 7197 2015
Size: 2776 1176 398 274 147 411 2352
Fragments arranged by size:
2776 2352 1176 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3798 3828 4287 4317 1178
Size: 30 165 30 2395 30 459 30 4395
Fragments arranged by size:
4395 2395 459 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3626 4275 1186
Size: 2440 649 4445
Fragments arranged by size:
4445 2440 649
BseYI C'CCAG_C
Cuts at: 6737 7233 6737
Size: 496 7038
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3003 3933 1163
Size: 573 1267 930 4764
Fragments arranged by size:
4764 1267 930 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4984 5745 6773 1540
Size: 275 186 2983 761 1028 2301
Fragments arranged by size:
2983 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3017 5595 5680 6751 3017
Size: 2578 85 1071 3800
Fragments arranged by size:
3800 2578 1071 85
BsmI GAATG_Cn'
Cuts at: 3226 5045 5138 3226
Size: 1819 93 5622
Fragments arranged by size:
5622 1819 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2074 2539 3017 4853 5595 5680 6751
Size: 399 284 465 478 1836 742 85 1071
Cuts at: 6751 1391
Size: 2174
Fragments arranged by size:
2174 1836 1071 742 478 465 399 284
85
BspEI T'CCGG_A
Cuts at: 4791 7197 4791
Size: 2406 5128
BspHI T'CATG_A
Cuts at: 3820 5423 7153 3820
Size: 1603 1730 4201
Fragments arranged by size:
4201 1730 1603
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4889 844
Size: 4045 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5421 1394
Size: 429 3598 3507
Fragments arranged by size:
3598 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6029 6203 65
Size: 1183 4781 174 1396
Fragments arranged by size:
4781 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6178 6365 1700
Size: 110 4368 187 2869
Fragments arranged by size:
4368 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7534
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7534
BssSI C'ACGA_G
Cuts at: 5594 6606 5594
Size: 1012 6522
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2235 794
Size: 1441 6093
BstBI TT'CG_AA
Cuts at: 6338 6338
Size: 7534
BstEII G'GTnAC_C
Cuts at: 1184 4694 1184
Size: 3510 4024
BstXI CCAn_nnnn'nTGG
Cuts at: 3591 3591
Size: 7534
BstYI r'GATC_y
Cuts at: 1972 2948 3191 3908 4398 4462 4519 4864 5231
Size: 976 243 717 490 64 57 345 367
Cuts at: 5231 5616 5633 7085 7171 7183 7529 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 976 717 490 385 367 346
345 243 86 64 57 17 12
BstZ17I GTA'TAC
Cuts at: 4451 4579 4451
Size: 128 7406
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6043
Fragments arranged by size:
6043 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6795
Fragments arranged by size:
6795 683 56
BtsI GCAGTG_nn'
Cuts at: 3015 5050 5844 5864 3015
Size: 2035 794 20 4685
Fragments arranged by size:
4685 2035 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7534
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7499
DraI TTT'AAA
Cuts at: 3789 4838 3789
Size: 1049 6485
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6541 809
Size: 507 5225 1802
Fragments arranged by size:
5225 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4660 4981 5870
Size: 54 1425 50 275 18 2830 321 889
Cuts at: 5870 7499 8
Size: 1629 43
Fragments arranged by size:
2830 1629 1425 889 321 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4981 1537
Size: 275 3169 4090
Fragments arranged by size:
4090 3169 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2679 3387 3396 5464 1353
Size: 1326 708 9 2068 3423
Fragments arranged by size:
3423 2068 1326 708 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6121 6495 6641 387
Size: 1064 138 4532 374 146 1280
Fragments arranged by size:
4532 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3315 3555 3621 3816 3825 4023 4044
Size: 393 1683 240 66 195 9 198 21
Cuts at: 4044 4308 4746 5597 6194 6981 7249 1239
Size: 264 438 851 597 787 268 1524
Fragments arranged by size:
1683 1524 851 787 597 438 393 268
264 240 198 195 66 21 9
EcoO109I rG'GnC_Cy
Cuts at: 4489 4489
Size: 7534
EcoRI G'AATT_C
Cuts at: 4843 5221 4843
Size: 378 7156
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3177 3320 3517 4077 4447 3177
Size: 143 197 560 370 6264
Fragments arranged by size:
6264 560 370 197 143
FspI TGC'GCA
Cuts at: 6040 6040
Size: 7534
HaeII r_GCGC'y
Cuts at: 1678 3502 6681 1678
Size: 1824 3179 2531
Fragments arranged by size:
3179 2531 1824
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2681 2713 4319 4351 6175
Size: 32 135 32 1109 32 1606 32 1824
Cuts at: 6175 6207 6249 6281 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1824 1606 1109 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2060 4375 4687 4878 5124 669
Size: 988 281 122 2315 312 191 246 3079
Fragments arranged by size:
3079 2315 988 312 281 246 191 122
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7534
HpaI GTT'AAC
Cuts at: 4687 5124 4687
Size: 437 7097
KpnI G_GTAC'C
Cuts at: 4859 4859
Size: 7534
MfeI C'AATT_G
Cuts at: 5133 5133
Size: 7534
MluI A'CGCG_T
Cuts at: 7410 7410
Size: 7534
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2907 4224 6648 6832 1305
Size: 1602 1317 2424 184 2007
Fragments arranged by size:
2424 2007 1602 1317 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4662 10
Size: 54 1425 343 2830 2882
Fragments arranged by size:
2882 2830 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2439 5492 5851 6010 518
Size: 648 1273 3053 359 159 2042
Fragments arranged by size:
3053 2042 1273 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3293 4950 5627 6775 7020 1606
Size: 1687 1657 677 1148 245 2120
Fragments arranged by size:
2120 1687 1657 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6043
Fragments arranged by size:
6043 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7534
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7534
NotI GC'GGCC_GC
Cuts at: 4981 4981
Size: 7534
NsiI A_TGCA'T
Cuts at: 3663 3663
Size: 7534
NspI r_CATG'y
Cuts at: 1132 1229 2852 3993 4658 4892 6437 1132
Size: 97 1623 1141 665 234 1545 2229
Fragments arranged by size:
2229 1623 1545 1141 665 234 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7534
PciI A'CATG_T
Cuts at: 3989 6433 3989
Size: 2444 5090
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7534
PmeI GTTT'AAAC
Cuts at: 4838 4838
Size: 7534
PmlI CAC'GTG
Cuts at: 2075 2075
Size: 7534
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5603 5635 7116 7148 5603
Size: 32 1481 32 5989
Fragments arranged by size:
5989 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4489 4489
Size: 7534
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7534
PsiI TTA'TAA
Cuts at: 2915 4776 5104 2915
Size: 1861 328 5345
Fragments arranged by size:
5345 1861 328
PstI C_TGCA'G
Cuts at: 830 4886 830
Size: 4056 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7534
PvuII CAG'CTG
Cuts at: 3293 3293
Size: 7534
SalI G'TCGA_C
Cuts at: 1936 2058 4876 1936
Size: 122 2818 4594
Fragments arranged by size:
4594 2818 122
SapI GCTCTTCn'nnn_
Cuts at: 3387 3387
Size: 7534
SbfI CC_TGCA'GG
Cuts at: 4886 4886
Size: 7534
ScaI AGT'ACT
Cuts at: 3144 4253 4317 4738 5782 7494 3144
Size: 1109 64 421 1044 1712 3184
Fragments arranged by size:
3184 1712 1109 1044 421 64
SfcI C'TryA_G
Cuts at: 826 1045 2041 2294 4882 6017 6698 6889 826
Size: 219 996 253 2588 1135 681 191 1471
Fragments arranged by size:
2588 1471 1135 996 681 253 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7534
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7534
SmaI CCC'GGG
Cuts at: 1872 4861 1872
Size: 2989 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5638 6539 6801 820
Size: 197 926 3695 901 262 1553
Fragments arranged by size:
3695 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5325 493
Size: 4832 2702
SpeI A'CTAG_T
Cuts at: 152 4186 152
Size: 4034 3500
SphI G_CATG'C
Cuts at: 1132 2852 4892 1132
Size: 1720 2040 3774
Fragments arranged by size:
3774 2040 1720
SspI AAT'ATT
Cuts at: 5 52 3715 4363 5458 5
Size: 47 3663 648 1095 2081
Fragments arranged by size:
3663 2081 1095 648 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7534
StyI C'CwwG_G
Cuts at: 513 1065 2004 4973 513
Size: 552 939 2969 3074
Fragments arranged by size:
3074 2969 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3123 3586 5561 5578 5731 5916 6361
Size: 1255 1543 463 1975 17 153 185 445
Cuts at: 6361 325
Size: 1498
Fragments arranged by size:
1975 1543 1498 1255 463 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2159 2591 3142
Size: 274 80 33 51 1443 182 432 551
Cuts at: 3142 3775 4251 4315 4706 4736 5780 7492 96
Size: 633 476 64 391 30 1044 1712 138
Fragments arranged by size:
1712 1443 1044 633 551 476 432 391
274 182 138 80 64 51 33 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4287 4319 7494 7526 4287
Size: 32 3175 32 4295
Fragments arranged by size:
4295 3175 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7534
XbaI T'CTAG_A
Cuts at: 3236 3905 4870 3236
Size: 669 965 5900
Fragments arranged by size:
5900 965 669
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3591 3591
Size: 7534
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7534
XmaI C'CCGG_G
Cuts at: 1870 4859 1870
Size: 2989 4545
XmnI GAAnn'nnTTC
Cuts at: 3571 4806 5663 3571
Size: 1235 857 5442
Fragments arranged by size:
5442 1235 857
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5342 276
Size: 53 83 186 936 3808 2468
Fragments arranged by size:
3808 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BbeI BbvCI BplI
Bpu10I BsiWI BsmBI Bsu36I DraIII EcoICRI EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI