Restriction map for
pFN21AE2201
(Circular) (Six-base) MAPSORT of: pFN21AE2201.seq Check: 8204 from: 1 to: 6838
pFN21AE2201 6838 bp
With 182 enzymes: SgfI *
November 2, 2012 18:20 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4648 278
Size: 53 83 186 936 3112 2468
Fragments arranged by size:
3112 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4181 1937
Size: 2244 4594
Acc65I G'GTAC_C
Cuts at: 4159 4159
Size: 6838
AclI AA'CG_TT
Cuts at: 1563 4965 5338 1563
Size: 3402 373 3063
Fragments arranged by size:
3402 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4901 6285 6553 1632
Size: 3269 1384 268 1917
Fragments arranged by size:
3269 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6641
AflIII A'CryG_T
Cuts at: 1203 5737 6714 1203
Size: 4534 977 1327
Fragments arranged by size:
4534 1327 977
AgeI A'CCGG_T
Cuts at: 5669 5669
Size: 6838
AhdI GACnn_n'nnGTC
Cuts at: 2638 4083 5567 2638
Size: 1445 1484 3909
Fragments arranged by size:
3909 1484 1445
AleI CACnn'nnGTG
Cuts at: 1166 2288 3309 1166
Size: 1122 1021 4695
Fragments arranged by size:
4695 1122 1021
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2999 3690 1184
Size: 1815 691 4332
Fragments arranged by size:
4332 1815 691
AlwNI CAG_nnn'CTG
Cuts at: 2603 3536 4088 4581 6153 2603
Size: 933 552 493 1572 3288
Fragments arranged by size:
3288 1572 933 552 493
ApaI G_GGCC'C
Cuts at: 2105 2177 2248 2660 3102 3129 3555 3570 3615
Size: 72 71 412 442 27 426 15 45
Cuts at: 3615 3773 2105
Size: 158 5170
Fragments arranged by size:
5170 442 426 412 158 72 71 45
27 15
ApaLI G'TGCA_C
Cuts at: 4895 6051 4895
Size: 1156 5682
ApoI r'AATT_y
Cuts at: 1493 4147 4373 4525 6634 1493
Size: 2654 226 152 2109 1697
Fragments arranged by size:
2654 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5392 160
Size: 872 4360 1606
Fragments arranged by size:
4360 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6838
AvaI C'yCGr_G
Cuts at: 1870 1943 2036 2152 2203 2567 2643 3101 3226
Size: 73 93 116 51 364 76 458 125
Cuts at: 3226 3587 3766 4163 1870
Size: 361 179 397 4545
Fragments arranged by size:
4545 458 397 364 361 179 125 116
93 76 73 51
BamHI G'GATC_C
Cuts at: 2866 4168 4535 2866
Size: 1302 367 5169
Fragments arranged by size:
5169 1302 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2078 2702 4159 5614 618
Size: 325 217 627 291 624 1457 1455 1842
Fragments arranged by size:
1842 1457 1455 627 624 325 291 217
BanII G_rGCy'C
Cuts at: 1391 2105 2177 2248 2596 2660 3102 3129 3555
Size: 714 72 71 348 64 442 27 426
Cuts at: 3555 3570 3595 3615 3773 3825 4003 4157 1391
Size: 15 25 20 158 52 178 154 4072
Fragments arranged by size:
4072 714 442 426 348 178 158 154
72 71 64 52 27 25 20 15
BbeI G_GCGC'C
Cuts at: 2082 2082
Size: 6838
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5867
BbvCI CC'TCA_GC
Cuts at: 3828 3828
Size: 6838
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3092 3126 5029 5063 1903
Size: 34 1155 34 1903 34 3678
Fragments arranged by size:
3678 1903 1155 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3063 4730 5940 3063
Size: 1667 1210 3961
Fragments arranged by size:
3961 1667 1210
BclI T'GATC_A
Cuts at: 1548 2478 3036 1548
Size: 930 558 5350
Fragments arranged by size:
5350 930 558
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2109 2700 3333
Size: 107 122 71 631 605 437 591 633
Cuts at: 3333 3525 3540 3731 3800 3809 4037 5449 136
Size: 192 15 191 69 9 228 1412 1525
Fragments arranged by size:
1525 1412 633 631 605 591 437 228
192 191 122 107 71 69 15 9
BglII A'GATC_T
Cuts at: 3381 6833 3381
Size: 3452 3386
BlpI GC'TnA_GC
Cuts at: 4255 4255
Size: 6838
Bme1580I G_kGCm'C
Cuts at: 1790 2105 2177 2248 2660 2994 3102 3129 3555
Size: 315 72 71 412 334 108 27 426
Cuts at: 3555 3570 3615 3773 4899 6055 1790
Size: 15 45 158 1126 1156 2573
Fragments arranged by size:
2573 1156 1126 426 412 334 315 158
108 72 71 45 27 15
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6838
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3500 3655 4569
Size: 416 53 83 399 24 2083 155 914
Cuts at: 4569 5527 442
Size: 958 1753
Fragments arranged by size:
2083 1753 958 914 416 399 155 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6838
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2532 2814 3158 3189 5498 1239
Size: 1293 282 344 31 2309 2579
Fragments arranged by size:
2579 2309 1293 344 282 31
Bpu10I CC'TnA_GC
Cuts at: 3813 3828 3813
Size: 15 6823
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4119 4963 5828 6126 4119
Size: 844 865 298 4831
Fragments arranged by size:
4831 865 844 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2759 5501 882
Size: 617 1260 2742 2219
Fragments arranged by size:
2742 2219 1260 617
BsaAI yAC'GTr
Cuts at: 493 1204 4629 493
Size: 711 3425 2702
Fragments arranged by size:
3425 2702 711
BsaBI GATnn'nnATC
Cuts at: 2349 2720 2906 6832 2349
Size: 371 186 3926 2355
Fragments arranged by size:
3926 2355 371 186
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2079 2232 4645 5027
Size: 53 83 186 936 546 153 2413 382
Cuts at: 5027 5662 275
Size: 635 1451
Fragments arranged by size:
2413 1451 936 635 546 382 186 153
83 53
BsaWI w'CCGG_w
Cuts at: 2356 2943 3983 5271 5669 5943 6090 6501 2356
Size: 587 1040 1288 398 274 147 411 2693
Fragments arranged by size:
2693 1288 1040 587 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3360 3390 1178
Size: 30 165 30 1957 30 4626
Fragments arranged by size:
4626 1957 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2505 3161 3609 3669 3965 1186
Size: 1319 656 448 60 296 4059
Fragments arranged by size:
4059 1319 656 448 296 60
BseYI C'CCAG_C
Cuts at: 3447 3642 3903 4014 4098 6041 6537 3447
Size: 195 261 111 84 1943 496 3748
Fragments arranged by size:
3748 1943 496 261 195 111 84
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2258 2729 2900 1163
Size: 573 522 471 171 5101
Fragments arranged by size:
5101 573 522 471 171
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2169 3328 3711 4288 5049 6077
Size: 275 186 168 1159 383 577 761 1028
Cuts at: 6077 1540
Size: 2301
Fragments arranged by size:
2301 1159 1028 761 577 383 275 186
168
BsiHKAI G_wGCw'C
Cuts at: 3147 4003 4899 4984 6055 3147
Size: 856 896 85 1071 3930
Fragments arranged by size:
3930 1071 896 856 85
BsmI GAATG_Cn'
Cuts at: 2998 4349 4442 2998
Size: 1351 93 5394
Fragments arranged by size:
5394 1351 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2105 2177 2248 2596 2660 2994 3102
Size: 399 315 72 71 348 64 334 108
Cuts at: 3102 3129 3147 3555 3570 3595 3615 3773 3825
Size: 27 18 408 15 25 20 158 52
Cuts at: 3825 4003 4157 4899 4984 6055 1391
Size: 178 154 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 408 399 348 334 315
178 158 154 108 85 72 71 64
52 27 25 20 18 15
BspEI T'CCGG_A
Cuts at: 6501 6501
Size: 6838
BspHI T'CATG_A
Cuts at: 2718 3682 4727 6457 2718
Size: 964 1045 1730 3099
Fragments arranged by size:
3099 1730 1045 964
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4193 844
Size: 3349 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4725 1394
Size: 429 2902 3507
Fragments arranged by size:
3507 2902 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5333 5507 65
Size: 1183 4085 174 1396
Fragments arranged by size:
4085 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2054 2738 3790 3808 3842 5482 5669
Size: 110 244 684 1052 18 34 1640 187
Cuts at: 5669 1700
Size: 2869
Fragments arranged by size:
2869 1640 1052 684 244 187 110 34
18
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6838
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6838
BssSI C'ACGA_G
Cuts at: 4898 5910 4898
Size: 1012 5826
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6838
BstBI TT'CG_AA
Cuts at: 5642 5642
Size: 6838
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6838
BstYI r'GATC_y
Cuts at: 1972 2822 2866 2957 3045 3381 4168 4535 4920
Size: 850 44 91 88 336 787 367 385
Cuts at: 4920 4937 6389 6475 6487 6833 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 850 787 385 367 346 336
91 88 86 44 17 12
Bsu36I CC'TnA_GG
Cuts at: 3182 3182
Size: 6838
BtgI C'CryG_G
Cuts at: 513 1065 2727 3711 513
Size: 552 1662 984 3640
Fragments arranged by size:
3640 1662 984 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2289 487
Size: 683 56 1063 5036
Fragments arranged by size:
5036 1063 683 56
BtsI GCAGTG_nn'
Cuts at: 2581 4354 5148 5168 2581
Size: 1773 794 20 4251
Fragments arranged by size:
4251 1773 794 20
ClaI AT'CG_AT
Cuts at: 1554 2979 1554
Size: 1425 5413
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6803
DraI TTT'AAA
Cuts at: 4142 4142
Size: 6838
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5845 809
Size: 507 4529 1802
Fragments arranged by size:
4529 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2166 3019 3325
Size: 54 1425 50 275 18 336 853 306
Cuts at: 3325 3708 3844 3892 3978 4285 5174 6803 8
Size: 383 136 48 86 307 889 1629 43
Fragments arranged by size:
1629 1425 889 853 383 336 307 306
275 136 86 54 50 48 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2166 3325 3708 4285 1537
Size: 275 354 1159 383 577 4090
Fragments arranged by size:
4090 1159 577 383 354 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2439 3241 3867 4768 1353
Size: 1086 802 626 901 3423
Fragments arranged by size:
3423 1086 901 802 626
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2085 2208 2985 4055 5425 5799
Size: 1064 138 496 123 777 1070 1370 374
Cuts at: 5799 5945 387
Size: 146 1280
Fragments arranged by size:
1370 1280 1070 1064 777 496 374 146
138 123
EcoICRI GAG'CTC
Cuts at: 4001 4001
Size: 6838
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2532 2814 3158 3189 4901 5498 6285
Size: 393 900 282 344 31 1712 597 787
Cuts at: 6285 6553 1239
Size: 268 1524
Fragments arranged by size:
1712 1524 900 787 597 393 344 282
268 31
EcoO109I rG'GnC_Cy
Cuts at: 2027 2090 2173 2174 2455 2656 2657 3098 3125
Size: 63 83 1 281 201 1 441 27
Cuts at: 3125 3126 3200 3266 3409 3494 3533 3551 3552
Size: 1 74 66 143 85 39 18 1
Cuts at: 3552 3567 3611 3612 3770 4085 2027
Size: 15 44 1 158 315 4780
Fragments arranged by size:
4780 441 315 281 201 158 143 85
83 74 66 63 44 39 27 18
15 1 1 1 1 1
EcoRI G'AATT_C
Cuts at: 4147 4525 4147
Size: 378 6460
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2824 2824
Size: 6838
FseI GG_CCGG'CC
Cuts at: 2742 2742
Size: 6838
FspI TGC'GCA
Cuts at: 5344 5344
Size: 6838
HaeII r_GCGC'y
Cuts at: 1678 2082 3920 5985 1678
Size: 404 1838 2065 2531
Fragments arranged by size:
2531 2065 1838 404
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2376 2408 2624 2656 2942
Size: 32 135 32 804 32 216 32 286
Cuts at: 2942 2974 3021 3053 3478 3510 4069 4101 5479
Size: 32 47 32 425 32 559 32 1378
Cuts at: 5479 5511 5553 5585 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1378 804 559 425 286 216 135
47 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4182 4428 669
Size: 988 281 2244 246 3079
Fragments arranged by size:
3079 2244 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6838
HpaI GTT'AAC
Cuts at: 4428 4428
Size: 6838
KasI G'GCGC_C
Cuts at: 2078 2078
Size: 6838
KpnI G_GTAC'C
Cuts at: 4163 4163
Size: 6838
MfeI C'AATT_G
Cuts at: 3259 4437 3259
Size: 1178 5660
MluI A'CGCG_T
Cuts at: 6714 6714
Size: 6838
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1989 2319 2539 3490 3831 5952 6136 1305
Size: 684 330 220 951 341 2121 184 2007
Fragments arranged by size:
2121 2007 951 684 341 330 220 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3980 10
Size: 54 1425 343 2148 2868
Fragments arranged by size:
2868 2148 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2288 2981 3309 4796 5155 5314 518
Size: 648 1122 693 328 1487 359 159 2042
Fragments arranged by size:
2042 1487 1122 693 648 359 328 159
MspA1I CmG'CkG
Cuts at: 1606 2242 2556 2729 2779 3394 3701 3707 3713
Size: 636 314 173 50 615 307 6 6
Cuts at: 3713 4254 4931 6079 6324 1606
Size: 541 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 636 615 541 314 307
245 173 50 6 6
NaeI GCC'GGC
Cuts at: 2056 2740 2056
Size: 684 6154
NarI GG'CG_CC
Cuts at: 2079 2079
Size: 6838
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6286
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6838
NgoMIV G'CCGG_C
Cuts at: 2054 2738 2054
Size: 684 6154
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6838
NotI GC'GGCC_GC
Cuts at: 3708 4285 3708
Size: 577 6261
NspI r_CATG'y
Cuts at: 1132 1229 3223 3605 4196 5741 1132
Size: 97 1994 382 591 1545 2229
Fragments arranged by size:
2229 1994 1545 591 382 97
PasI CC'CwG_GG
Cuts at: 1348 2380 3637 1348
Size: 1032 1257 4549
Fragments arranged by size:
4549 1257 1032
PciI A'CATG_T
Cuts at: 5737 5737
Size: 6838
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4088 1108
Size: 2980 3858
PfoI T'CCnGG_A
Cuts at: 2913 3994 2913
Size: 1081 5757
PmeI GTTT'AAAC
Cuts at: 4142 4142
Size: 6838
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4907 4939 6420 6452 4907
Size: 32 1481 32 5293
Fragments arranged by size:
5293 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2027 2455 3200 3266 3409 3494 4085 2027
Size: 428 745 66 143 85 591 4780
Fragments arranged by size:
4780 745 591 428 143 85 66
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6838
PsiI TTA'TAA
Cuts at: 4408 4408
Size: 6838
PspOMI G'GGCC_C
Cuts at: 2101 2173 2244 2656 3098 3125 3551 3566 3611
Size: 72 71 412 442 27 426 15 45
Cuts at: 3611 3769 2101
Size: 158 5170
Fragments arranged by size:
5170 442 426 412 158 72 71 45
27 15
PstI C_TGCA'G
Cuts at: 830 2784 3706 4190 830
Size: 1954 922 484 3478
Fragments arranged by size:
3478 1954 922 484
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6838
PvuII CAG'CTG
Cuts at: 2779 3394 3701 2779
Size: 615 307 5916
Fragments arranged by size:
5916 615 307
SacI G_AGCT'C
Cuts at: 4003 4003
Size: 6838
SacII CC_GC'GG
Cuts at: 2730 3714 2730
Size: 984 5854
SalI G'TCGA_C
Cuts at: 1936 4180 1936
Size: 2244 4594
SanDI GG'GwC_CC
Cuts at: 2455 3266 3409 2455
Size: 811 143 5884
Fragments arranged by size:
5884 811 143
SbfI CC_TGCA'GG
Cuts at: 4190 4190
Size: 6838
ScaI AGT'ACT
Cuts at: 3956 5086 6798 3956
Size: 1130 1712 3996
Fragments arranged by size:
3996 1712 1130
SfcI C'TryA_G
Cuts at: 826 1045 2014 2780 3702 4024 4186 5321 6002
Size: 219 969 766 922 322 162 1135 681
Cuts at: 6002 6193 826
Size: 191 1471
Fragments arranged by size:
1471 1135 969 922 766 681 322 219
191 162
SfiI GGCCn_nnn'nGGCC
Cuts at: 3525 3540 3800 3525
Size: 15 260 6563
Fragments arranged by size:
6563 260 15
SfoI GGC'GCC
Cuts at: 2080 2080
Size: 6838
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6838
SgrAI Cr'CCGG_yG
Cuts at: 1700 2054 1700
Size: 354 6484
SmaI CCC'GGG
Cuts at: 1872 2038 2154 2205 3228 3589 3768 4165 1872
Size: 166 116 51 1023 361 179 397 4545
Fragments arranged by size:
4545 1023 397 361 179 166 116 51
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4134 4942 5843 6105 820
Size: 197 926 2191 808 901 262 1553
Fragments arranged by size:
2191 1553 926 901 808 262 197
SnaBI TAC'GTA
Cuts at: 493 4629 493
Size: 4136 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6838
SphI G_CATG'C
Cuts at: 1132 3223 4196 1132
Size: 2091 973 3774
Fragments arranged by size:
3774 2091 973
SrfI GCCC'GGGC
Cuts at: 2038 2154 2038
Size: 116 6722
SspI AAT'ATT
Cuts at: 5 52 4046 4762 5
Size: 47 3994 716 2081
Fragments arranged by size:
3994 2081 716 47
StuI AGG'CCT
Cuts at: 1517 3866 1517
Size: 2349 4489
StyI C'CwwG_G
Cuts at: 513 1065 2426 2926 3253 4277 513
Size: 552 1361 500 327 1024 3074
Fragments arranged by size:
3074 1361 1024 552 500 327
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3415 3853 4865 4882 5035 5220 5665
Size: 1255 1835 438 1012 17 153 185 445
Cuts at: 5665 325
Size: 1498
Fragments arranged by size:
1835 1498 1255 1012 445 438 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2448 2536 3954
Size: 274 80 33 51 1443 471 88 1418
Cuts at: 3954 5084 6796 96
Size: 1130 1712 138
Fragments arranged by size:
1712 1443 1418 1130 471 274 138 88
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6798 6830 6798
Size: 32 6806
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6838
XbaI T'CTAG_A
Cuts at: 4174 4174
Size: 6838
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2623 2933 3056 3639 2623
Size: 310 123 583 5822
Fragments arranged by size:
5822 583 310 123
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6838
XmaI C'CCGG_G
Cuts at: 1870 2036 2152 2203 3226 3587 3766 4163 1870
Size: 166 116 51 1023 361 179 397 4545
Fragments arranged by size:
4545 1023 397 361 179 166 116 51
XmnI GAAnn'nnTTC
Cuts at: 4967 4967
Size: 6838
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4646 276
Size: 53 83 186 936 3112 2468
Fragments arranged by size:
3112 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SacII SalI SanDI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FspAI
NruI NsiI PacI PmlI PspXI PsrI RsrII SapI
SexAI SwaI