Restriction map for
pFN21AE2199
(Circular) (Six-base) MAPSORT of: pFN21AE2199.seq Check: 1648 from: 1 to: 4984
pFN21AE2199 4984 bp
With 182 enzymes: SgfI *
March 28, 2012 12:14 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2794 278
Size: 53 83 186 936 1258 2468
Fragments arranged by size:
2468 1258 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2327 1937
Size: 390 4594
Acc65I G'GTAC_C
Cuts at: 2305 2305
Size: 4984
AclI AA'CG_TT
Cuts at: 1563 3111 3484 1563
Size: 1548 373 3063
Fragments arranged by size:
3063 1548 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2095 3047 4431 4699 1632
Size: 463 952 1384 268 1917
Fragments arranged by size:
1917 1384 952 463 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4787
AflIII A'CryG_T
Cuts at: 1203 3883 4860 1203
Size: 2680 977 1327
Fragments arranged by size:
2680 1327 977
AgeI A'CCGG_T
Cuts at: 3815 3815
Size: 4984
AhdI GACnn_n'nnGTC
Cuts at: 3713 3713
Size: 4984
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 4984
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2241 1184
Size: 1057 3927
AlwNI CAG_nnn'CTG
Cuts at: 2727 4299 2727
Size: 1572 3412
ApaLI G'TGCA_C
Cuts at: 3041 4197 3041
Size: 1156 3828
ApoI r'AATT_y
Cuts at: 1493 2140 2293 2519 2671 4780 1493
Size: 647 153 226 152 2109 1697
Fragments arranged by size:
2109 1697 647 226 153 152
AseI AT'TA_AT
Cuts at: 160 1032 3538 160
Size: 872 2506 1606
Fragments arranged by size:
2506 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4984
AvaI C'yCGr_G
Cuts at: 1870 1943 2309 1870
Size: 73 366 4545
Fragments arranged by size:
4545 366 73
BamHI G'GATC_C
Cuts at: 2314 2681 2314
Size: 367 4617
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2305 3760 618
Size: 325 217 627 518 1455 1842
Fragments arranged by size:
1842 1455 627 518 325 217
BanII G_rGCy'C
Cuts at: 1391 2303 1391
Size: 912 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4013
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3175 3209 1903
Size: 34 50 34 1154 34 3678
Fragments arranged by size:
3678 1154 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2876 4086 2876
Size: 1210 3774
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4984
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3595 136
Size: 107 122 71 631 605 1923 1525
Fragments arranged by size:
1923 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4979 4979
Size: 4984
BlpI GC'TnA_GC
Cuts at: 2401 2401
Size: 4984
Bme1580I G_kGCm'C
Cuts at: 1790 3045 4201 1790
Size: 1255 1156 2573
Fragments arranged by size:
2573 1255 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 4984
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2181 2229 2715
Size: 416 53 83 399 24 764 48 486
Cuts at: 2715 3673 442
Size: 958 1753
Fragments arranged by size:
1753 958 764 486 416 399 83 53
48 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 4984
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2291 3644 1239
Size: 1052 1353 2579
Fragments arranged by size:
2579 1353 1052
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3109 3974 4272 3109
Size: 865 298 3821
Fragments arranged by size:
3821 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2017 3647 882
Size: 617 518 1630 2219
Fragments arranged by size:
2219 1630 617 518
BsaAI yAC'GTr
Cuts at: 493 1204 2775 493
Size: 711 1571 2702
Fragments arranged by size:
2702 1571 711
BsaBI GATnn'nnATC
Cuts at: 4978 4978
Size: 4984
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2791 3173 3808 275
Size: 53 83 186 936 1258 382 635 1451
Fragments arranged by size:
1451 1258 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3417 3815 4089 4236 4647 3417
Size: 398 274 147 411 3754
Fragments arranged by size:
3754 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4759
Fragments arranged by size:
4759 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2027 2255 1186
Size: 841 228 3915
Fragments arranged by size:
3915 841 228
BseYI C'CCAG_C
Cuts at: 4187 4683 4187
Size: 496 4488
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2026 2058 1163
Size: 573 290 32 4089
Fragments arranged by size:
4089 573 290 32
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2100 2434 3195 4223 1540
Size: 275 186 99 334 761 1028 2301
Fragments arranged by size:
2301 1028 761 334 275 186 99
BsiHKAI G_wGCw'C
Cuts at: 2180 3045 3130 4201 2180
Size: 865 85 1071 2963
Fragments arranged by size:
2963 1071 865 85
BsmI GAATG_Cn'
Cuts at: 2495 2588 2495
Size: 93 4891
BsmBI CGTCTCn'nnnn_
Cuts at: 2156 2156
Size: 4984
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2180 2303 3045 3130 4201 1391
Size: 399 390 123 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 390 123 85
BspEI T'CCGG_A
Cuts at: 4647 4647
Size: 4984
BspHI T'CATG_A
Cuts at: 2873 4603 2873
Size: 1730 3254
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 1999 2209 2339 844
Size: 1155 210 130 3489
Fragments arranged by size:
3489 1155 210 130
BsrBI CCG'CTC
Cuts at: 1394 1823 2871 1394
Size: 429 1048 3507
Fragments arranged by size:
3507 1048 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3479 3653 65
Size: 1183 2231 174 1396
Fragments arranged by size:
2231 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2214 3628 3815 1700
Size: 110 404 1414 187 2869
Fragments arranged by size:
2869 1414 404 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 4984
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 4984
BssSI C'ACGA_G
Cuts at: 3044 4056 3044
Size: 1012 3972
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2081 2175 794
Size: 1287 94 3603
Fragments arranged by size:
3603 1287 94
BstBI TT'CG_AA
Cuts at: 3788 3788
Size: 4984
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 4984
BstYI r'GATC_y
Cuts at: 1972 2314 2681 3066 3083 4535 4621 4633 4979
Size: 342 367 385 17 1452 86 12 346
Cuts at: 4979 1972
Size: 1977
Fragments arranged by size:
1977 1452 385 367 346 342 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2046 513
Size: 552 981 3451
Fragments arranged by size:
3451 981 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4245
Fragments arranged by size:
4245 683 56
BtsI GCAGTG_nn'
Cuts at: 2016 2500 3294 3314 2016
Size: 484 794 20 3686
Fragments arranged by size:
3686 794 484 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 4984
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4949
DraI TTT'AAA
Cuts at: 2288 2288
Size: 4984
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3991 809
Size: 507 2675 1802
Fragments arranged by size:
2675 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2431 3320 4949
Size: 54 1425 50 275 18 601 889 1629
Cuts at: 4949 8
Size: 43
Fragments arranged by size:
1629 1425 889 601 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2431 1537
Size: 275 619 4090
Fragments arranged by size:
4090 619 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2914 1353
Size: 1561 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3571 3945 4091 387
Size: 1064 138 1982 374 146 1280
Fragments arranged by size:
1982 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2095 2291 3047 3644 4431 4699 1239
Size: 393 463 196 756 597 787 268 1524
Fragments arranged by size:
1524 787 756 597 463 393 268 196
EcoO109I rG'GnC_Cy
Cuts at: 2065 2065
Size: 4984
EcoRI G'AATT_C
Cuts at: 2293 2671 2293
Size: 378 4606
FspI TGC'GCA
Cuts at: 3490 3490
Size: 4984
HaeII r_GCGC'y
Cuts at: 1678 4131 1678
Size: 2453 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3625 3657 3699 3731 1373
Size: 32 135 32 2053 32 42 32 2626
Fragments arranged by size:
2626 2053 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2328 2574 669
Size: 988 281 390 246 3079
Fragments arranged by size:
3079 988 390 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 4984
HpaI GTT'AAC
Cuts at: 2574 2574
Size: 4984
KpnI G_GTAC'C
Cuts at: 2309 2309
Size: 4984
MfeI C'AATT_G
Cuts at: 2583 2583
Size: 4984
MluI A'CGCG_T
Cuts at: 4860 4860
Size: 4984
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4098 4282 1305
Size: 2793 184 2007
Fragments arranged by size:
2793 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3162
Fragments arranged by size:
3162 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2942 3301 3460 518
Size: 648 1776 359 159 2042
Fragments arranged by size:
2042 1776 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2400 3077 4225 4470 1606
Size: 794 677 1148 245 2120
Fragments arranged by size:
2120 1148 794 677 245
NaeI GCC'GGC
Cuts at: 2216 2216
Size: 4984
NcoI C'CATG_G
Cuts at: 513 1065 2046 513
Size: 552 981 3451
Fragments arranged by size:
3451 981 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 4984
NgoMIV G'CCGG_C
Cuts at: 2214 2214
Size: 4984
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 4984
NotI GC'GGCC_GC
Cuts at: 2431 2431
Size: 4984
NspI r_CATG'y
Cuts at: 1132 1229 2342 3887 1132
Size: 97 1113 1545 2229
Fragments arranged by size:
2229 1545 1113 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 4984
PciI A'CATG_T
Cuts at: 3883 3883
Size: 4984
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 4984
PmeI GTTT'AAAC
Cuts at: 2288 2288
Size: 4984
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3053 3085 4566 4598 3053
Size: 32 1481 32 3439
Fragments arranged by size:
3439 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2065 2065
Size: 4984
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 4984
PsiI TTA'TAA
Cuts at: 2554 2554
Size: 4984
PstI C_TGCA'G
Cuts at: 830 2336 830
Size: 1506 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 4984
SalI G'TCGA_C
Cuts at: 1936 2326 1936
Size: 390 4594
SbfI CC_TGCA'GG
Cuts at: 2336 2336
Size: 4984
ScaI AGT'ACT
Cuts at: 3232 4944 3232
Size: 1712 3272
SexAI A'CCwGG_T
Cuts at: 2061 2061
Size: 4984
SfcI C'TryA_G
Cuts at: 826 1045 2332 3467 4148 4339 826
Size: 219 1287 1135 681 191 1471
Fragments arranged by size:
1471 1287 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4984
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 4984
SmaI CCC'GGG
Cuts at: 1872 2311 1872
Size: 439 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3088 3989 4251 820
Size: 197 926 1145 901 262 1553
Fragments arranged by size:
1553 1145 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2775 493
Size: 2282 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 4984
SphI G_CATG'C
Cuts at: 1132 2342 1132
Size: 1210 3774
SspI AAT'ATT
Cuts at: 5 52 2908 5
Size: 47 2856 2081
Fragments arranged by size:
2856 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 4984
StyI C'CwwG_G
Cuts at: 513 1065 2046 2423 513
Size: 552 981 377 3074
Fragments arranged by size:
3074 981 552 377
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3011 3028 3181 3366 3811 325
Size: 1255 1431 17 153 185 445 1498
Fragments arranged by size:
1498 1431 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3230 4942 96
Size: 274 80 33 51 1443 1253 1712 138
Fragments arranged by size:
1712 1443 1253 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4944 4976 4944
Size: 32 4952
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 4984
XbaI T'CTAG_A
Cuts at: 2320 2320
Size: 4984
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 4984
XmaI C'CCGG_G
Cuts at: 1870 2309 1870
Size: 439 4545
XmnI GAAnn'nnTTC
Cuts at: 3113 3113
Size: 4984
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2792 276
Size: 53 83 186 936 1258 2468
Fragments arranged by size:
2468 1258 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SfiI
SfoI SrfI SwaI XcmI