Restriction map for
pFN21AE2195
(Circular) (Six-base) MAPSORT of: pFN21AE2195.seq Check: 6891 from: 1 to: 5959
pFN21AE2195 5959 bp
With 182 enzymes: SgfI *
January 30, 2014 17:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3769 278
Size: 53 83 186 936 2233 2468
Fragments arranged by size:
2468 2233 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3302 1937
Size: 1365 4594
Acc65I G'GTAC_C
Cuts at: 3280 3280
Size: 5959
AclI AA'CG_TT
Cuts at: 1563 4086 4459 1563
Size: 2523 373 3063
Fragments arranged by size:
3063 2523 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4022 5406 5674 1632
Size: 2390 1384 268 1917
Fragments arranged by size:
2390 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5762
AflIII A'CryG_T
Cuts at: 1203 2516 4858 5835 1203
Size: 1313 2342 977 1327
Fragments arranged by size:
2342 1327 1313 977
AgeI A'CCGG_T
Cuts at: 4790 4790
Size: 5959
AhdI GACnn_n'nnGTC
Cuts at: 4688 4688
Size: 5959
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5959
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2514 1184
Size: 1330 4629
AlwNI CAG_nnn'CTG
Cuts at: 2132 3702 5274 2132
Size: 1570 1572 2817
Fragments arranged by size:
2817 1572 1570
ApaLI G'TGCA_C
Cuts at: 4016 5172 4016
Size: 1156 4803
ApoI r'AATT_y
Cuts at: 1493 3268 3494 3646 5755 1493
Size: 1775 226 152 2109 1697
Fragments arranged by size:
2109 1775 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3110 3222 4513 160
Size: 872 2078 112 1291 1606
Fragments arranged by size:
2078 1606 1291 872 112
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
AvaI C'yCGr_G
Cuts at: 1870 1943 2016 2084 2615 3284 1870
Size: 73 73 68 531 669 4545
Fragments arranged by size:
4545 669 531 73 73 68
BamHI G'GATC_C
Cuts at: 3028 3289 3656 3028
Size: 261 367 5331
Fragments arranged by size:
5331 367 261
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2080 2238 2485 3280 4735
Size: 325 217 627 293 158 247 795 1455
Cuts at: 4735 618
Size: 1842
Fragments arranged by size:
1842 1455 795 627 325 293 247 217
158
BanII G_rGCy'C
Cuts at: 1391 2430 2721 3278 1391
Size: 1039 291 557 4072
Fragments arranged by size:
4072 1039 557 291
BbeI G_GCGC'C
Cuts at: 2084 2242 2489 2084
Size: 158 247 5554
Fragments arranged by size:
5554 247 158
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2519 3100 928
Size: 971 620 581 3787
Fragments arranged by size:
3787 971 620 581
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2158 2192 2383 2417 3057 3091 4150
Size: 34 221 34 191 34 640 34 1059
Cuts at: 4150 4184 1903
Size: 34 3678
Fragments arranged by size:
3678 1059 640 221 191 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3851 5061 3851
Size: 1210 4749
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5959
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2483 4570 136
Size: 107 122 71 631 605 811 2087 1525
Fragments arranged by size:
2087 1525 811 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3021 5954 3021
Size: 2933 3026
BlpI GC'TnA_GC
Cuts at: 3376 3376
Size: 5959
Bme1580I G_kGCm'C
Cuts at: 1790 2818 4020 5176 1790
Size: 1028 1202 1156 2573
Fragments arranged by size:
2573 1202 1156 1028
BmgBI CAC'GTC
Cuts at: 1318 2622 1318
Size: 1304 4655
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2686 2812 3690
Size: 416 53 83 399 24 1269 126 878
Cuts at: 3690 4648 442
Size: 958 1753
Fragments arranged by size:
1753 1269 958 878 416 399 126 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5959
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2109 4619 1239
Size: 870 2510 2579
Fragments arranged by size:
2579 2510 870
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4084 4949 5247 4084
Size: 865 298 4796
Fragments arranged by size:
4796 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2006 2895 4622 882
Size: 617 507 889 1727 2219
Fragments arranged by size:
2219 1727 889 617 507
BsaAI yAC'GTr
Cuts at: 493 1204 3750 493
Size: 711 2546 2702
Fragments arranged by size:
2702 2546 711
BsaBI GATnn'nnATC
Cuts at: 5953 5953
Size: 5959
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2081 2239 2486 3766
Size: 53 83 186 936 548 158 247 1280
Cuts at: 3766 4148 4783 275
Size: 382 635 1451
Fragments arranged by size:
1451 1280 936 635 548 382 247 186
158 83 53
BsaWI w'CCGG_w
Cuts at: 4392 4790 5064 5211 5622 4392
Size: 398 274 147 411 4729
Fragments arranged by size:
4729 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2107 2137 1178
Size: 30 165 30 704 30 5000
Fragments arranged by size:
5000 704 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2422 1186
Size: 1236 4723
BseYI C'CCAG_C
Cuts at: 2584 5162 5658 2584
Size: 2578 496 2885
Fragments arranged by size:
2885 2578 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2029 2377 3135 1163
Size: 573 293 348 758 3987
Fragments arranged by size:
3987 758 573 348 293
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2331 2838 3409 4170 5198 1540
Size: 275 186 330 507 571 761 1028 2301
Fragments arranged by size:
2301 1028 761 571 507 330 275 186
BsiHKAI G_wGCw'C
Cuts at: 2370 2562 2946 4020 4105 5176 2370
Size: 192 384 1074 85 1071 3153
Fragments arranged by size:
3153 1074 1071 384 192 85
BsmI GAATG_Cn'
Cuts at: 2981 3470 3563 2981
Size: 489 93 5377
Fragments arranged by size:
5377 489 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2834 2834
Size: 5959
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2370 2430 2562 2721 2818 2946 3278
Size: 399 580 60 132 159 97 128 332
Cuts at: 3278 4020 4105 5176 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 580 399 332 159 132
128 97 85 60
BspEI T'CCGG_A
Cuts at: 5622 5622
Size: 5959
BspHI T'CATG_A
Cuts at: 3848 5578 3848
Size: 1730 4229
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3051 3314 844
Size: 2207 263 3489
Fragments arranged by size:
3489 2207 263
BsrBI CCG'CTC
Cuts at: 1394 1823 2467 2896 3846 1394
Size: 429 644 429 950 3507
Fragments arranged by size:
3507 950 644 429 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4454 4628 65
Size: 1183 3206 174 1396
Fragments arranged by size:
3206 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2235 2578 4603 4790 1700
Size: 110 425 343 2025 187 2869
Fragments arranged by size:
2869 2025 425 343 187 110
BsrGI T'GTAC_A
Cuts at: 96 3228 96
Size: 3132 2827
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5959
BssSI C'ACGA_G
Cuts at: 2219 3209 4019 5031 2219
Size: 990 810 1012 3147
Fragments arranged by size:
3147 1012 990 810
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5959
BstBI TT'CG_AA
Cuts at: 3067 4763 3067
Size: 1696 4263
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5959
BstXI CCAn_nnnn'nTGG
Cuts at: 2455 2455
Size: 5959
BstYI r'GATC_y
Cuts at: 1972 3021 3028 3057 3289 3656 4041 4058 5510
Size: 1049 7 29 232 367 385 17 1452
Cuts at: 5510 5596 5608 5954 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1049 385 367 346 232 86
29 17 12 7
BtgI C'CryG_G
Cuts at: 513 1065 2157 2454 2796 513
Size: 552 1092 297 342 3676
Fragments arranged by size:
3676 1092 552 342 297
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2428 487
Size: 683 56 1202 4018
Fragments arranged by size:
4018 1202 683 56
BtsI GCAGTG_nn'
Cuts at: 2944 3475 4269 4289 2944
Size: 531 794 20 4614
Fragments arranged by size:
4614 794 531 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5959
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5924
DraI TTT'AAA
Cuts at: 3263 3263
Size: 5959
DraIII CAC_nnn'GTG
Cuts at: 3158 3158
Size: 5959
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4966 809
Size: 507 3650 1802
Fragments arranged by size:
3650 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2208 2457 2475
Size: 54 1425 50 275 18 378 249 18
Cuts at: 2475 2553 2754 2844 3406 4295 5924 8
Size: 78 201 90 562 889 1629 43
Fragments arranged by size:
1629 1425 889 562 378 275 249 201
90 78 54 50 43 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 3406 1537
Size: 275 1594 4090
Fragments arranged by size:
4090 1594 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2671 3889 1353
Size: 1318 1218 3423
Fragments arranged by size:
3423 1318 1218
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2083 4546 4920 5066 387
Size: 1064 138 494 2463 374 146 1280
Fragments arranged by size:
2463 1280 1064 494 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2109 4022 4619 5406 5674 1239
Size: 393 477 1913 597 787 268 1524
Fragments arranged by size:
1913 1524 787 597 477 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2767 2767
Size: 5959
EcoRI G'AATT_C
Cuts at: 3268 3646 3268
Size: 378 5581
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2281 2281
Size: 5959
FspI TGC'GCA
Cuts at: 4465 4465
Size: 5959
HaeII r_GCGC'y
Cuts at: 1678 2084 2242 2294 2454 2474 2489 2729 5106
Size: 406 158 52 160 20 15 240 2377
Cuts at: 5106 1678
Size: 2531
Fragments arranged by size:
2531 2377 406 240 160 158 52 20
15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2132 2164 2911 2943 3013
Size: 32 135 32 560 32 747 32 70
Cuts at: 3013 3045 4600 4632 4674 4706 1373
Size: 32 1555 32 42 32 2626
Fragments arranged by size:
2626 1555 747 560 135 70 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3303 3549 669
Size: 988 281 1365 246 3079
Fragments arranged by size:
3079 1365 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5959
HpaI GTT'AAC
Cuts at: 3549 3549
Size: 5959
KasI G'GCGC_C
Cuts at: 2080 2238 2485 2080
Size: 158 247 5554
Fragments arranged by size:
5554 247 158
KpnI G_GTAC'C
Cuts at: 3284 3284
Size: 5959
MfeI C'AATT_G
Cuts at: 3558 3558
Size: 5959
MluI A'CGCG_T
Cuts at: 2516 5835 2516
Size: 3319 2640
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5073 5257 1305
Size: 3768 184 2007
Fragments arranged by size:
3768 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2210 2477 2756 10
Size: 54 1425 343 378 267 279 3213
Fragments arranged by size:
3213 1425 378 343 279 267 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2072 2339 2496 2870 3224 3917 4276
Size: 648 906 267 157 374 354 693 359
Cuts at: 4276 4435 518
Size: 159 2042
Fragments arranged by size:
2042 906 693 648 374 359 354 267
159 157
MspA1I CmG'CkG
Cuts at: 1606 2781 3375 4052 5200 5445 1606
Size: 1175 594 677 1148 245 2120
Fragments arranged by size:
2120 1175 1148 677 594 245
NarI GG'CG_CC
Cuts at: 2081 2239 2486 2081
Size: 158 247 5554
Fragments arranged by size:
5554 247 158
NcoI C'CATG_G
Cuts at: 513 1065 2454 513
Size: 552 1389 4018
Fragments arranged by size:
4018 1389 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5959
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5959
NotI GC'GGCC_GC
Cuts at: 3406 3406
Size: 5959
NspI r_CATG'y
Cuts at: 1132 1229 2077 3317 4862 1132
Size: 97 848 1240 1545 2229
Fragments arranged by size:
2229 1545 1240 848 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5959
PciI A'CATG_T
Cuts at: 4858 4858
Size: 5959
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2132 3025 1108
Size: 1024 893 4042
Fragments arranged by size:
4042 1024 893
PmeI GTTT'AAAC
Cuts at: 3263 3263
Size: 5959
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4028 4060 5541 5573 4028
Size: 32 1481 32 4414
Fragments arranged by size:
4414 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5959
PsiI TTA'TAA
Cuts at: 3529 3529
Size: 5959
PstI C_TGCA'G
Cuts at: 830 3119 3311 830
Size: 2289 192 3478
Fragments arranged by size:
3478 2289 192
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5959
SalI G'TCGA_C
Cuts at: 1936 3301 1936
Size: 1365 4594
SapI GCTCTTCn'nnn_
Cuts at: 2671 2671
Size: 5959
SbfI CC_TGCA'GG
Cuts at: 3311 3311
Size: 5959
ScaI AGT'ACT
Cuts at: 4207 5919 4207
Size: 1712 4247
SexAI A'CCwGG_T
Cuts at: 2301 2301
Size: 5959
SfcI C'TryA_G
Cuts at: 826 1045 3115 3307 4442 5123 5314 826
Size: 219 2070 192 1135 681 191 1471
Fragments arranged by size:
2070 1471 1135 681 219 192 191
SfoI GGC'GCC
Cuts at: 2082 2240 2487 2082
Size: 158 247 5554
Fragments arranged by size:
5554 247 158
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5959
SmaI CCC'GGG
Cuts at: 1872 2018 2617 3286 1872
Size: 146 599 669 4545
Fragments arranged by size:
4545 669 599 146
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4063 4964 5226 820
Size: 197 926 2120 901 262 1553
Fragments arranged by size:
2120 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3750 493
Size: 3257 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5959
SphI G_CATG'C
Cuts at: 1132 3317 1132
Size: 2185 3774
SspI AAT'ATT
Cuts at: 5 52 3883 5
Size: 47 3831 2081
Fragments arranged by size:
3831 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5959
StyI C'CwwG_G
Cuts at: 513 1065 2454 2790 3032 3398 513
Size: 552 1389 336 242 366 3074
Fragments arranged by size:
3074 1389 552 366 336 242
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2852 2869 3986 4003 4156 4341 4786
Size: 1255 1272 17 1117 17 153 185 445
Cuts at: 4786 325
Size: 1498
Fragments arranged by size:
1498 1272 1255 1117 445 185 153 17
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2598 3166 3228
Size: 274 80 33 51 1443 621 568 62
Cuts at: 3228 4205 5917 96
Size: 977 1712 138
Fragments arranged by size:
1712 1443 977 621 568 274 138 80
62 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2107 2139 5919 5951 2107
Size: 32 3780 32 2115
Fragments arranged by size:
3780 2115 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2303 1316
Size: 987 4972
XbaI T'CTAG_A
Cuts at: 3295 3295
Size: 5959
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2767 2854 2767
Size: 87 5872
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5959
XmaI C'CCGG_G
Cuts at: 1870 2016 2615 3284 1870
Size: 146 599 669 4545
Fragments arranged by size:
4545 669 599 146
XmnI GAAnn'nnTTC
Cuts at: 2264 4088 2264
Size: 1824 4135
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3767 276
Size: 53 83 186 936 2233 2468
Fragments arranged by size:
2468 2233 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoNI
EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SfiI SrfI SwaI