Restriction map for
pFN21AE2191
(Circular) (Six-base) MAPSORT of: pFN21AE2191.seq Check: 5770 from: 1 to: 5806
pFN21AE2191 5806 bp
With 182 enzymes: SgfI *
April 19, 2016 16:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3616 278
Size: 53 83 186 936 2080 2468
Fragments arranged by size:
2468 2080 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2658 3149 1937
Size: 721 491 4594
Fragments arranged by size:
4594 721 491
Acc65I G'GTAC_C
Cuts at: 3127 3127
Size: 5806
AclI AA'CG_TT
Cuts at: 1563 3933 4306 1563
Size: 2370 373 3063
Fragments arranged by size:
3063 2370 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2286 2790 3869 5253 5521 1632
Size: 654 504 1079 1384 268 1917
Fragments arranged by size:
1917 1384 1079 654 504 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5609
AflIII A'CryG_T
Cuts at: 1203 4705 5682 1203
Size: 3502 977 1327
Fragments arranged by size:
3502 1327 977
AgeI A'CCGG_T
Cuts at: 4637 4637
Size: 5806
AhdI GACnn_n'nnGTC
Cuts at: 4535 4535
Size: 5806
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5806
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5806
AlwNI CAG_nnn'CTG
Cuts at: 3549 5121 3549
Size: 1572 4234
ApaLI G'TGCA_C
Cuts at: 2784 3863 5019 2784
Size: 1079 1156 3571
Fragments arranged by size:
3571 1156 1079
ApoI r'AATT_y
Cuts at: 1493 2282 2375 2995 3115 3341 3493 5602 1493
Size: 789 93 620 120 226 152 2109 1697
Fragments arranged by size:
2109 1697 789 620 226 152 120 93
AseI AT'TA_AT
Cuts at: 160 1032 2150 4360 160
Size: 872 1118 2210 1606
Fragments arranged by size:
2210 1606 1118 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5806
AvaI C'yCGr_G
Cuts at: 1870 1943 3131 1870
Size: 73 1188 4545
Fragments arranged by size:
4545 1188 73
AvrII C'CTAG_G
Cuts at: 2831 2831
Size: 5806
BamHI G'GATC_C
Cuts at: 3136 3503 3136
Size: 367 5439
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3127 4582 618
Size: 325 217 627 1340 1455 1842
Fragments arranged by size:
1842 1455 1340 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3125 1391
Size: 1734 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2262 2796 928
Size: 971 363 534 3938
Fragments arranged by size:
3938 971 534 363
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3997 4031 1903
Size: 34 2060 34 3678
Fragments arranged by size:
3678 2060 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3698 4908 3698
Size: 1210 4596
BclI T'GATC_A
Cuts at: 1548 2041 1548
Size: 493 5313
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4417 136
Size: 107 122 71 631 605 2745 1525
Fragments arranged by size:
2745 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5801 5801
Size: 5806
BlpI GC'TnA_GC
Cuts at: 3223 3223
Size: 5806
Bme1580I G_kGCm'C
Cuts at: 1790 2788 3867 5023 1790
Size: 998 1079 1156 2573
Fragments arranged by size:
2573 1156 1079 998
BmgBI CAC'GTC
Cuts at: 1318 2050 1318
Size: 732 5074
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3537 4495 442
Size: 416 53 83 399 24 2120 958 1753
Fragments arranged by size:
2120 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5806
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2355 3030 4466 1239
Size: 1116 675 1436 2579
Fragments arranged by size:
2579 1436 1116 675
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3931 4796 5094 3931
Size: 865 298 4643
Fragments arranged by size:
4643 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4469 882
Size: 617 2970 2219
Fragments arranged by size:
2970 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2461 3597 493
Size: 711 1257 1136 2702
Fragments arranged by size:
2702 1257 1136 711
BsaBI GATnn'nnATC
Cuts at: 5800 5800
Size: 5806
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 3613 3995 4630
Size: 53 83 186 936 476 1604 382 635
Cuts at: 4630 275
Size: 1451
Fragments arranged by size:
1604 1451 936 635 476 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4239 4637 4911 5058 5469 4239
Size: 398 274 147 411 4576
Fragments arranged by size:
4576 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5581
Fragments arranged by size:
5581 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2614 2665 1186
Size: 1428 51 4327
Fragments arranged by size:
4327 1428 51
BseYI C'CCAG_C
Cuts at: 2248 5009 5505 2248
Size: 2761 496 2549
Fragments arranged by size:
2761 2549 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5233
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3256 4017 5045 1540
Size: 275 186 1255 761 1028 2301
Fragments arranged by size:
2301 1255 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2668 2788 3867 3952 5023 2668
Size: 120 1079 85 1071 3451
Fragments arranged by size:
3451 1079 1071 120 85
BsmI GAATG_Cn'
Cuts at: 3317 3410 3317
Size: 93 5713
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2668 2788 3125 3867 3952 5023 1391
Size: 399 878 120 337 742 85 1071 2174
Fragments arranged by size:
2174 1071 878 742 399 337 120 85
BspEI T'CCGG_A
Cuts at: 5469 5469
Size: 5806
BspHI T'CATG_A
Cuts at: 2178 3695 5425 2178
Size: 1517 1730 2559
Fragments arranged by size:
2559 1730 1517
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3161 844
Size: 2317 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2016 3693 1394
Size: 429 193 1677 3507
Fragments arranged by size:
3507 1677 429 193
BsrDI GCAATG_nn'
Cuts at: 65 1248 4301 4475 65
Size: 1183 3053 174 1396
Fragments arranged by size:
3053 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4450 4637 1700
Size: 110 2640 187 2869
Fragments arranged by size:
2869 2640 187 110
BsrGI T'GTAC_A
Cuts at: 96 3067 96
Size: 2971 2835
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5806
BssSI C'ACGA_G
Cuts at: 2103 3866 4878 2103
Size: 1763 1012 3031
Fragments arranged by size:
3031 1763 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5806
BstBI TT'CG_AA
Cuts at: 4610 4610
Size: 5806
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5806
BstYI r'GATC_y
Cuts at: 1972 3136 3503 3888 3905 5357 5443 5455 5801
Size: 1164 367 385 17 1452 86 12 346
Cuts at: 5801 1972
Size: 1977
Fragments arranged by size:
1977 1452 1164 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4315
Fragments arranged by size:
4315 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5067
Fragments arranged by size:
5067 683 56
BtsI GCAGTG_nn'
Cuts at: 3322 4116 4136 3322
Size: 794 20 4992
Fragments arranged by size:
4992 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5806
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5771
DraI TTT'AAA
Cuts at: 3110 3110
Size: 5806
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4813 809
Size: 507 3497 1802
Fragments arranged by size:
3497 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3253 4142 5771
Size: 54 1425 50 275 18 1423 889 1629
Cuts at: 5771 8
Size: 43
Fragments arranged by size:
1629 1425 1423 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3253 1537
Size: 275 1441 4090
Fragments arranged by size:
4090 1441 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2860 3736 1353
Size: 1507 876 3423
Fragments arranged by size:
3423 1507 876
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4393 4767 4913 387
Size: 1064 138 2804 374 146 1280
Fragments arranged by size:
2804 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2286 2355 2790 3030 3869 4466 5253
Size: 393 654 69 435 240 839 597 787
Cuts at: 5253 5521 1239
Size: 268 1524
Fragments arranged by size:
1524 839 787 654 597 435 393 268
240 69
EcoO109I rG'GnC_Cy
Cuts at: 2651 2651
Size: 5806
EcoRI G'AATT_C
Cuts at: 3115 3493 3115
Size: 378 5428
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2291 2291
Size: 5806
FspI TGC'GCA
Cuts at: 4312 4312
Size: 5806
HaeII r_GCGC'y
Cuts at: 1678 4953 1678
Size: 3275 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2507 2539 2920 2952 4447
Size: 32 135 32 935 32 381 32 1495
Cuts at: 4447 4479 4521 4553 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1495 935 381 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2818 3150 3396 669
Size: 988 281 880 332 246 3079
Fragments arranged by size:
3079 988 880 332 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5806
HpaI GTT'AAC
Cuts at: 3396 3396
Size: 5806
KpnI G_GTAC'C
Cuts at: 3131 3131
Size: 5806
MfeI C'AATT_G
Cuts at: 2217 3405 2217
Size: 1188 4618
MluI A'CGCG_T
Cuts at: 5682 5682
Size: 5806
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4920 5104 1305
Size: 3615 184 2007
Fragments arranged by size:
3615 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3984
Fragments arranged by size:
3984 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3764 4123 4282 518
Size: 648 2598 359 159 2042
Fragments arranged by size:
2598 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3222 3899 5047 5292 1606
Size: 1616 677 1148 245 2120
Fragments arranged by size:
2120 1616 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4315
Fragments arranged by size:
4315 939 552
NdeI CA'TA_TG
Cuts at: 387 2425 387
Size: 2038 3768
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5806
NotI GC'GGCC_GC
Cuts at: 3253 3253
Size: 5806
NspI r_CATG'y
Cuts at: 1132 1229 3164 4709 1132
Size: 97 1935 1545 2229
Fragments arranged by size:
2229 1935 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5806
PciI A'CATG_T
Cuts at: 4705 4705
Size: 5806
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5806
PmeI GTTT'AAAC
Cuts at: 3110 3110
Size: 5806
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3875 3907 5388 5420 3875
Size: 32 1481 32 4261
Fragments arranged by size:
4261 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2651 2651
Size: 5806
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5806
PsiI TTA'TAA
Cuts at: 2718 3376 2718
Size: 658 5148
PstI C_TGCA'G
Cuts at: 830 2569 2850 3158 830
Size: 1739 281 308 3478
Fragments arranged by size:
3478 1739 308 281
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5806
SalI G'TCGA_C
Cuts at: 1936 3148 1936
Size: 1212 4594
SbfI CC_TGCA'GG
Cuts at: 3158 3158
Size: 5806
ScaI AGT'ACT
Cuts at: 2144 4054 5766 2144
Size: 1910 1712 2184
Fragments arranged by size:
2184 1910 1712
SfcI C'TryA_G
Cuts at: 826 1045 2565 2655 2846 3154 4289 4970 5161
Size: 219 1520 90 191 308 1135 681 191
Cuts at: 5161 826
Size: 1471
Fragments arranged by size:
1520 1471 1135 681 308 219 191 191
90
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5806
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5806
SmaI CCC'GGG
Cuts at: 1872 3133 1872
Size: 1261 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3910 4811 5073 820
Size: 197 926 1967 901 262 1553
Fragments arranged by size:
1967 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3597 493
Size: 3104 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5806
SphI G_CATG'C
Cuts at: 1132 3164 1132
Size: 2032 3774
SspI AAT'ATT
Cuts at: 5 52 3730 5
Size: 47 3678 2081
Fragments arranged by size:
3678 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5806
StyI C'CwwG_G
Cuts at: 513 1065 2004 2831 3245 513
Size: 552 939 827 414 3074
Fragments arranged by size:
3074 939 827 552 414
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3833 3850 4003 4188 4633 325
Size: 1255 2253 17 153 185 445 1498
Fragments arranged by size:
2253 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2142 2226 3067
Size: 274 80 33 51 1443 165 84 841
Cuts at: 3067 4052 5764 96
Size: 985 1712 138
Fragments arranged by size:
1712 1443 985 841 274 165 138 84
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5766 5798 5766
Size: 32 5774
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5806
XbaI T'CTAG_A
Cuts at: 2236 3142 2236
Size: 906 4900
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2320 2320
Size: 5806
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5806
XmaI C'CCGG_G
Cuts at: 1870 3131 1870
Size: 1261 4545
XmnI GAAnn'nnTTC
Cuts at: 3935 3935
Size: 5806
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3614 276
Size: 53 83 186 936 2080 2468
Fragments arranged by size:
2468 2080 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI