Restriction map for
pFN21AE2190
(Circular) (Six-base) MAPSORT of: pFN21AE2190.seq Check: 8734 from: 1 to: 6298
pFN21AE2190 6298 bp
With 182 enzymes: SgfI *
August 16, 2010 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4108 278
Size: 53 83 186 936 2572 2468
Fragments arranged by size:
2572 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3288 3641 1937
Size: 1351 353 4594
Fragments arranged by size:
4594 1351 353
Acc65I G'GTAC_C
Cuts at: 3619 3619
Size: 6298
AclI AA'CG_TT
Cuts at: 1563 4425 4798 1563
Size: 2862 373 3063
Fragments arranged by size:
3063 2862 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2342 2547 3458 4361 5745 6013 1632
Size: 710 205 911 903 1384 268 1917
Fragments arranged by size:
1917 1384 911 903 710 268 205
AfeI AGC'GCT
Cuts at: 2338 2338
Size: 6298
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6101
AflIII A'CryG_T
Cuts at: 1203 5197 6174 1203
Size: 3994 977 1327
Fragments arranged by size:
3994 1327 977
AgeI A'CCGG_T
Cuts at: 5129 5129
Size: 6298
AhdI GACnn_n'nnGTC
Cuts at: 5027 5027
Size: 6298
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6298
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3369 1184
Size: 2185 4113
AlwNI CAG_nnn'CTG
Cuts at: 2243 2828 3154 4041 5613 2243
Size: 585 326 887 1572 2928
Fragments arranged by size:
2928 1572 887 585 326
ApaLI G'TGCA_C
Cuts at: 2204 2793 4355 5511 2204
Size: 589 1562 1156 2991
Fragments arranged by size:
2991 1562 1156 589
ApoI r'AATT_y
Cuts at: 1493 2734 3158 3607 3833 3985 6094 1493
Size: 1241 424 449 226 152 2109 1697
Fragments arranged by size:
2109 1697 1241 449 424 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4852 160
Size: 872 3820 1606
Fragments arranged by size:
3820 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6298
AvaI C'yCGr_G
Cuts at: 1870 1943 2667 3623 1870
Size: 73 724 956 4545
Fragments arranged by size:
4545 956 724 73
BamHI G'GATC_C
Cuts at: 2833 3460 3628 3995 2833
Size: 627 168 367 5136
Fragments arranged by size:
5136 627 367 168
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3619 5074 618
Size: 325 217 627 1832 1455 1842
Fragments arranged by size:
1842 1832 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3617 1391
Size: 2226 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2120 2272 2348 3002 928
Size: 971 221 152 76 654 4224
Fragments arranged by size:
4224 971 654 221 152 76
BbvCI CC'TCA_GC
Cuts at: 2652 2652
Size: 6298
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4489 4523 1903
Size: 34 2552 34 3678
Fragments arranged by size:
3678 2552 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4190 5400 4190
Size: 1210 5088
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6298
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4909 136
Size: 107 122 71 631 605 3237 1525
Fragments arranged by size:
3237 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2281 2585 6293 2281
Size: 304 3708 2286
Fragments arranged by size:
3708 2286 304
BlpI GC'TnA_GC
Cuts at: 2018 3715 2018
Size: 1697 4601
Bme1580I G_kGCm'C
Cuts at: 1790 2208 2797 3254 4359 5515 1790
Size: 418 589 457 1105 1156 2573
Fragments arranged by size:
2573 1156 1105 589 457 418
BmgBI CAC'GTC
Cuts at: 1318 2014 2234 1318
Size: 696 220 5382
Fragments arranged by size:
5382 696 220
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3328 4029 4987
Size: 416 53 83 399 24 1911 701 958
Cuts at: 4987 442
Size: 1753
Fragments arranged by size:
1911 1753 958 701 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6298
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4958 1239
Size: 3719 2579
Bpu10I CC'TnA_GC
Cuts at: 2652 2652
Size: 6298
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2355 3024 3297 3610 4423 5288 5586 2355
Size: 669 273 313 813 865 298 3067
Fragments arranged by size:
3067 865 813 669 313 298 273
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4961 882
Size: 617 3462 2219
Fragments arranged by size:
3462 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4089 493
Size: 711 2885 2702
Fragments arranged by size:
2885 2702 711
BsaBI GATnn'nnATC
Cuts at: 6292 6292
Size: 6298
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4105 4487 5122 275
Size: 53 83 186 936 2572 382 635 1451
Fragments arranged by size:
2572 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4731 5129 5403 5550 5961 4731
Size: 398 274 147 411 5068
Fragments arranged by size:
5068 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2061 2091 2989 3019 1178
Size: 30 165 30 658 30 898 30 4457
Fragments arranged by size:
4457 898 658 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2650 1186
Size: 1464 4834
BseYI C'CCAG_C
Cuts at: 2863 3015 5501 5997 2863
Size: 152 2486 496 3164
Fragments arranged by size:
3164 2486 496 152
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2256 3365 1163
Size: 573 520 1109 4096
Fragments arranged by size:
4096 1109 573 520
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3748 4509 5537 1540
Size: 275 186 1747 761 1028 2301
Fragments arranged by size:
2301 1747 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2208 2797 4359 4444 5515 2208
Size: 589 1562 85 1071 2991
Fragments arranged by size:
2991 1562 1071 589 85
BsmI GAATG_Cn'
Cuts at: 3809 3902 3809
Size: 93 6205
BsmBI CGTCTCn'nnnn_
Cuts at: 2064 2064
Size: 6298
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2208 2797 3254 3617 4359 4444 5515
Size: 399 418 589 457 363 742 85 1071
Cuts at: 5515 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 589 457 418 399 363
85
BspEI T'CCGG_A
Cuts at: 5961 5961
Size: 6298
BspHI T'CATG_A
Cuts at: 4187 5917 4187
Size: 1730 4568
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2818 3653 844
Size: 1974 835 3489
Fragments arranged by size:
3489 1974 835
BsrBI CCG'CTC
Cuts at: 1394 1823 4185 1394
Size: 429 2362 3507
Fragments arranged by size:
3507 2362 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4793 4967 65
Size: 1183 3545 174 1396
Fragments arranged by size:
3545 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4942 5129 1700
Size: 110 3132 187 2869
Fragments arranged by size:
3132 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2497 96
Size: 2401 3897
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6298
BssSI C'ACGA_G
Cuts at: 4358 5370 4358
Size: 1012 5286
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6298
BstBI TT'CG_AA
Cuts at: 5102 5102
Size: 6298
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6298
BstXI CCAn_nnnn'nTGG
Cuts at: 2572 2572
Size: 6298
BstYI r'GATC_y
Cuts at: 1972 2281 2585 2833 3071 3460 3628 3995 4380
Size: 309 304 248 238 389 168 367 385
Cuts at: 4380 4397 5849 5935 5947 6293 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 389 385 367 346 309 304
248 238 168 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2950 3377 513
Size: 552 939 946 427 3434
Fragments arranged by size:
3434 946 939 552 427
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5559
Fragments arranged by size:
5559 683 56
BtsI GCAGTG_nn'
Cuts at: 3252 3814 4608 4628 3252
Size: 562 794 20 4922
Fragments arranged by size:
4922 794 562 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6298
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6263
DraI TTT'AAA
Cuts at: 3602 3602
Size: 6298
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5305 809
Size: 507 3989 1802
Fragments arranged by size:
3989 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3374 3745 4634
Size: 54 1425 50 275 18 1544 371 889
Cuts at: 4634 6263 8
Size: 1629 43
Fragments arranged by size:
1629 1544 1425 889 371 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3745 1537
Size: 275 1933 4090
Fragments arranged by size:
4090 1933 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2427 4228 1353
Size: 1074 1801 3423
Fragments arranged by size:
3423 1801 1074
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 4885 5259 5405 387
Size: 1064 138 435 2861 374 146 1280
Fragments arranged by size:
2861 1280 1064 435 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2342 2547 3458 4361 4958 5745 6013
Size: 393 710 205 911 903 597 787 268
Cuts at: 6013 1239
Size: 1524
Fragments arranged by size:
1524 911 903 787 710 597 393 268
205
EcoNI CCTnn'n_nnAGG
Cuts at: 3146 3146
Size: 6298
EcoO109I rG'GnC_Cy
Cuts at: 3151 3350 3151
Size: 199 6099
EcoRI G'AATT_C
Cuts at: 3607 3985 3607
Size: 378 5920
EcoRV GAT'ATC
Cuts at: 2294 2294
Size: 6298
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2303 2303
Size: 6298
FspI TGC'GCA
Cuts at: 2846 4804 2846
Size: 1958 4340
HaeII r_GCGC'y
Cuts at: 1678 2340 5445 1678
Size: 662 3105 2531
Fragments arranged by size:
3105 2531 662
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2577 2609 2661 2693 2969
Size: 32 135 32 1005 32 52 32 276
Cuts at: 2969 2989 3001 3021 4939 4971 5013 5045 1373
Size: 20 12 20 1918 32 42 32 2626
Fragments arranged by size:
2626 1918 1005 276 135 52 42 32
32 32 32 32 32 20 20 12
HincII GTy'rAC
Cuts at: 669 1657 1938 2313 3197 3642 3888 669
Size: 988 281 375 884 445 246 3079
Fragments arranged by size:
3079 988 884 445 375 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6298
HpaI GTT'AAC
Cuts at: 3888 3888
Size: 6298
KpnI G_GTAC'C
Cuts at: 3623 3623
Size: 6298
MfeI C'AATT_G
Cuts at: 3897 3897
Size: 6298
MluI A'CGCG_T
Cuts at: 6174 6174
Size: 6298
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2254 5412 5596 1305
Size: 949 3158 184 2007
Fragments arranged by size:
3158 2007 949 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3376 10
Size: 54 1425 343 1544 2932
Fragments arranged by size:
2932 1544 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4256 4615 4774 518
Size: 648 3090 359 159 2042
Fragments arranged by size:
3090 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2867 3714 4391 5539 5784 1606
Size: 1261 847 677 1148 245 2120
Fragments arranged by size:
2120 1261 1148 847 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2950 3377 513
Size: 552 939 946 427 3434
Fragments arranged by size:
3434 946 939 552 427
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6298
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6298
NotI GC'GGCC_GC
Cuts at: 3745 3745
Size: 6298
NspI r_CATG'y
Cuts at: 1132 1229 3656 5201 1132
Size: 97 2427 1545 2229
Fragments arranged by size:
2427 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6298
PciI A'CATG_T
Cuts at: 5197 5197
Size: 6298
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6298
PmeI GTTT'AAAC
Cuts at: 3602 3602
Size: 6298
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4367 4399 5880 5912 4367
Size: 32 1481 32 4753
Fragments arranged by size:
4753 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3151 3350 3151
Size: 199 6099
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6298
PsiI TTA'TAA
Cuts at: 3868 3868
Size: 6298
PstI C_TGCA'G
Cuts at: 830 2389 3013 3103 3650 830
Size: 1559 624 90 547 3478
Fragments arranged by size:
3478 1559 624 547 90
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6298
PvuII CAG'CTG
Cuts at: 2867 2867
Size: 6298
SalI G'TCGA_C
Cuts at: 1936 3640 1936
Size: 1704 4594
SbfI CC_TGCA'GG
Cuts at: 3650 3650
Size: 6298
ScaI AGT'ACT
Cuts at: 2165 4546 6258 2165
Size: 2381 1712 2205
Fragments arranged by size:
2381 2205 1712
SfcI C'TryA_G
Cuts at: 826 1045 2385 3009 3099 3146 3646 4781 5462
Size: 219 1340 624 90 47 500 1135 681
Cuts at: 5462 5653 826
Size: 191 1471
Fragments arranged by size:
1471 1340 1135 681 624 500 219 191
90 47
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6298
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6298
SmaI CCC'GGG
Cuts at: 1872 3625 1872
Size: 1753 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2370 3039 3312 3589 4402 5303
Size: 197 926 427 669 273 277 813 901
Cuts at: 5303 5565 820
Size: 262 1553
Fragments arranged by size:
1553 926 901 813 669 427 277 273
262 197
SnaBI TAC'GTA
Cuts at: 493 4089 493
Size: 3596 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6298
SphI G_CATG'C
Cuts at: 1132 3656 1132
Size: 2524 3774
SspI AAT'ATT
Cuts at: 5 52 2251 4222 5
Size: 47 2199 1971 2081
Fragments arranged by size:
2199 2081 1971 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6298
StyI C'CwwG_G
Cuts at: 513 1065 2004 2950 3377 3737 513
Size: 552 939 946 427 360 3074
Fragments arranged by size:
3074 946 939 552 427 360
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3097 4325 4342 4495 4680 5125 325
Size: 1255 1517 1228 17 153 185 445 1498
Fragments arranged by size:
1517 1498 1255 1228 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2034 2163 2497
Size: 274 80 33 51 1443 57 129 334
Cuts at: 2497 4544 6256 96
Size: 2047 1712 138
Fragments arranged by size:
2047 1712 1443 334 274 138 129 80
57 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2061 2093 2420 2452 6258 6290 2061
Size: 32 327 32 3806 32 2069
Fragments arranged by size:
3806 2069 327 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6298
XbaI T'CTAG_A
Cuts at: 2605 3634 2605
Size: 1029 5269
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6298
XmaI C'CCGG_G
Cuts at: 1870 3623 1870
Size: 1753 4545
XmnI GAAnn'nnTTC
Cuts at: 2250 4427 2250
Size: 2177 4121
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4106 276
Size: 53 83 186 936 2572 2468
Fragments arranged by size:
2572 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI