Restriction map for
pFN21AE2188
(Circular) (Six-base) MAPSORT of: pFN21AE2188.seq Check: 720 from: 1 to: 5974
pFN21AE2188 5974 bp
With 182 enzymes: SgfI *
August 20, 2012 11:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3784 278
Size: 53 83 186 936 2248 2468
Fragments arranged by size:
2468 2248 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2128 2679 2993 3317 1937
Size: 191 551 314 324 4594
Fragments arranged by size:
4594 551 324 314 191
Acc65I G'GTAC_C
Cuts at: 3295 3295
Size: 5974
AclI AA'CG_TT
Cuts at: 1563 4101 4474 1563
Size: 2538 373 3063
Fragments arranged by size:
3063 2538 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2242 2567 2638 4037 5421 5689 1632
Size: 610 325 71 1399 1384 268 1917
Fragments arranged by size:
1917 1399 1384 610 325 268 71
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5777
AflIII A'CryG_T
Cuts at: 1203 4873 5850 1203
Size: 3670 977 1327
Fragments arranged by size:
3670 1327 977
AgeI A'CCGG_T
Cuts at: 4805 4805
Size: 5974
AhdI GACnn_n'nnGTC
Cuts at: 4703 4703
Size: 5974
AleI CACnn'nnGTG
Cuts at: 1166 2783 3172 1166
Size: 1617 389 3968
Fragments arranged by size:
3968 1617 389
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5974
AlwNI CAG_nnn'CTG
Cuts at: 2213 2276 3717 5289 2213
Size: 63 1441 1572 2898
Fragments arranged by size:
2898 1572 1441 63
ApaI G_GGCC'C
Cuts at: 2029 2868 2029
Size: 839 5135
ApaLI G'TGCA_C
Cuts at: 4031 5187 4031
Size: 1156 4818
ApoI r'AATT_y
Cuts at: 1493 3021 3283 3509 3661 5770 1493
Size: 1528 262 226 152 2109 1697
Fragments arranged by size:
2109 1697 1528 262 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4528 160
Size: 872 3496 1606
Fragments arranged by size:
3496 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5974
AvaI C'yCGr_G
Cuts at: 1870 1943 3299 1870
Size: 73 1356 4545
Fragments arranged by size:
4545 1356 73
BamHI G'GATC_C
Cuts at: 3304 3671 3304
Size: 367 5607
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3295 4750 618
Size: 325 217 627 1508 1455 1842
Fragments arranged by size:
1842 1508 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2029 2051 2316 2868 3293 1391
Size: 638 22 265 552 425 4072
Fragments arranged by size:
4072 638 552 425 265 22
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2026 928
Size: 971 127 4876
Fragments arranged by size:
4876 971 127
BbvCI CC'TCA_GC
Cuts at: 2364 2364
Size: 5974
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4165 4199 1903
Size: 34 2228 34 3678
Fragments arranged by size:
3678 2228 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3866 5076 3866
Size: 1210 4764
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5974
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4585 136
Size: 107 122 71 631 605 2913 1525
Fragments arranged by size:
2913 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3162 5969 3162
Size: 2807 3167
BlpI GC'TnA_GC
Cuts at: 3391 3391
Size: 5974
Bme1580I G_kGCm'C
Cuts at: 1790 2029 2868 4035 5191 1790
Size: 239 839 1167 1156 2573
Fragments arranged by size:
2573 1167 1156 839 239
BmgBI CAC'GTC
Cuts at: 1318 2032 1318
Size: 714 5260
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2959 3104 3705
Size: 416 53 83 399 24 1542 145 601
Cuts at: 3705 4663 442
Size: 958 1753
Fragments arranged by size:
1753 1542 958 601 416 399 145 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5974
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2372 2957 3104 3229 4634 1239
Size: 1133 585 147 125 1405 2579
Fragments arranged by size:
2579 1405 1133 585 147 125
Bpu10I CC'TnA_GC
Cuts at: 2364 2946 3192 2364
Size: 582 246 5146
Fragments arranged by size:
5146 582 246
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2510 2701 4099 4964 5262 2510
Size: 191 1398 865 298 3222
Fragments arranged by size:
3222 1398 865 298 191
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2620 4637 882
Size: 617 1121 2017 2219
Fragments arranged by size:
2219 2017 1121 617
BsaAI yAC'GTr
Cuts at: 493 1204 2781 3765 493
Size: 711 1577 984 2702
Fragments arranged by size:
2702 1577 984 711
BsaBI GATnn'nnATC
Cuts at: 5968 5968
Size: 5974
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2980 3781 4163 4798
Size: 53 83 186 936 1447 801 382 635
Cuts at: 4798 275
Size: 1451
Fragments arranged by size:
1451 1447 936 801 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4407 4805 5079 5226 5637 4407
Size: 398 274 147 411 4744
Fragments arranged by size:
4744 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2822 2852 3202 3232 1178
Size: 30 165 30 1419 30 350 30 3920
Fragments arranged by size:
3920 1419 350 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2156 2381 1186
Size: 970 225 4779
Fragments arranged by size:
4779 970 225
BseYI C'CCAG_C
Cuts at: 2100 2502 2711 2751 2820 3088 5177 5673 2100
Size: 402 209 40 69 268 2089 496 2401
Fragments arranged by size:
2401 2089 496 402 268 209 69 40
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5401
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2061 2883 3424 4185 5213 1540
Size: 275 186 60 822 541 761 1028 2301
Fragments arranged by size:
2301 1028 822 761 541 275 186 60
BsiHKAI G_wGCw'C
Cuts at: 2769 4035 4120 5191 2769
Size: 1266 85 1071 3552
Fragments arranged by size:
3552 1266 1071 85
BsmI GAATG_Cn'
Cuts at: 3485 3578 3485
Size: 93 5881
BsmBI CGTCTCn'nnnn_
Cuts at: 2987 2987
Size: 5974
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2029 2051 2316 2769 2868 3293 4035
Size: 399 239 22 265 453 99 425 742
Cuts at: 4035 4120 5191 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 453 425 399 265 239
99 85 22
BspEI T'CCGG_A
Cuts at: 5637 5637
Size: 5974
BspHI T'CATG_A
Cuts at: 3863 5593 3863
Size: 1730 4244
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3329 844
Size: 2485 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3243 3861 1394
Size: 429 1420 618 3507
Fragments arranged by size:
3507 1420 618 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4469 4643 65
Size: 1183 3221 174 1396
Fragments arranged by size:
3221 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4618 4805 1700
Size: 110 2808 187 2869
Fragments arranged by size:
2869 2808 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5974
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5974
BssSI C'ACGA_G
Cuts at: 4034 5046 4034
Size: 1012 4962
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5974
BstBI TT'CG_AA
Cuts at: 4778 4778
Size: 5974
BstEII G'GTnAC_C
Cuts at: 1184 2224 1184
Size: 1040 4934
BstYI r'GATC_y
Cuts at: 1972 3162 3304 3671 4056 4073 5525 5611 5623
Size: 1190 142 367 385 17 1452 86 12
Cuts at: 5623 5969 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1190 385 367 346 142 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2406 513
Size: 552 939 402 4081
Fragments arranged by size:
4081 939 552 402
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2797 487
Size: 683 56 1571 3664
Fragments arranged by size:
3664 1571 683 56
BtsI GCAGTG_nn'
Cuts at: 2476 2584 3490 4284 4304 2476
Size: 108 906 794 20 4146
Fragments arranged by size:
4146 906 794 108 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5974
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5939
DraI TTT'AAA
Cuts at: 3278 3278
Size: 5974
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4981 809
Size: 507 3665 1802
Fragments arranged by size:
3665 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2198 2442 2622
Size: 54 1425 50 275 18 368 244 180
Cuts at: 2622 3181 3421 4310 5939 8
Size: 559 240 889 1629 43
Fragments arranged by size:
1629 1425 889 559 368 275 244 240
180 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3421 1537
Size: 275 1609 4090
Fragments arranged by size:
4090 1609 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2441 2774 3904 1353
Size: 1088 333 1130 3423
Fragments arranged by size:
3423 1130 1088 333
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3062 4561 4935 5081 387
Size: 1064 138 1473 1499 374 146 1280
Fragments arranged by size:
1499 1473 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2242 2372 2567 2638 2957 3104 3229
Size: 393 610 130 195 71 319 147 125
Cuts at: 3229 4037 4634 5421 5689 1239
Size: 808 597 787 268 1524
Fragments arranged by size:
1524 808 787 610 597 393 319 268
195 147 130 125 71
EcoO109I rG'GnC_Cy
Cuts at: 2025 2824 2864 3213 2025
Size: 799 40 349 4786
Fragments arranged by size:
4786 799 349 40
EcoRI G'AATT_C
Cuts at: 3021 3283 3661 3021
Size: 262 378 5334
Fragments arranged by size:
5334 378 262
FspI TGC'GCA
Cuts at: 4480 4480
Size: 5974
HaeII r_GCGC'y
Cuts at: 1678 5121 1678
Size: 3443 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2110 2142 2234 2266 2357
Size: 32 135 32 538 32 92 32 91
Cuts at: 2357 2389 2822 2854 4615 4647 4689 4721 1373
Size: 32 433 32 1761 32 42 32 2626
Fragments arranged by size:
2626 1761 538 433 135 92 91 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2994 3318 3564 669
Size: 988 281 1056 324 246 3079
Fragments arranged by size:
3079 1056 988 324 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5974
HpaI GTT'AAC
Cuts at: 3564 3564
Size: 5974
KpnI G_GTAC'C
Cuts at: 3299 3299
Size: 5974
MfeI C'AATT_G
Cuts at: 3573 3573
Size: 5974
MluI A'CGCG_T
Cuts at: 5850 5850
Size: 5974
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5088 5272 1305
Size: 3783 184 2007
Fragments arranged by size:
3783 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2200 2444 3183 10
Size: 54 1425 343 368 244 739 2801
Fragments arranged by size:
2801 1425 739 368 343 244 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2783 2924 3172 3183 3268 3932 4291
Size: 648 1617 141 248 11 85 664 359
Cuts at: 4291 4450 518
Size: 159 2042
Fragments arranged by size:
2042 1617 664 648 359 248 159 141
85 11
MspA1I CmG'CkG
Cuts at: 1606 2104 2506 2658 3390 4067 5215 5460 1606
Size: 498 402 152 732 677 1148 245 2120
Fragments arranged by size:
2120 1148 732 677 498 402 245 152
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4483
Fragments arranged by size:
4483 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5974
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5974
NotI GC'GGCC_GC
Cuts at: 3421 3421
Size: 5974
NspI r_CATG'y
Cuts at: 1132 1229 2077 3332 4877 1132
Size: 97 848 1255 1545 2229
Fragments arranged by size:
2229 1545 1255 848 97
PasI CC'CwG_GG
Cuts at: 1348 2161 1348
Size: 813 5161
PciI A'CATG_T
Cuts at: 4873 4873
Size: 5974
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3017 1108
Size: 1909 4065
PmeI GTTT'AAAC
Cuts at: 3278 3278
Size: 5974
PmlI CAC'GTG
Cuts at: 2781 2781
Size: 5974
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4043 4075 5556 5588 4043
Size: 32 1481 32 4429
Fragments arranged by size:
4429 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2824 3213 2824
Size: 389 5585
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5974
PsiI TTA'TAA
Cuts at: 3544 3544
Size: 5974
PspOMI G'GGCC_C
Cuts at: 2025 2864 2025
Size: 839 5135
PstI C_TGCA'G
Cuts at: 830 2591 3326 830
Size: 1761 735 3478
Fragments arranged by size:
3478 1761 735
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5974
PvuII CAG'CTG
Cuts at: 2104 2506 2658 2104
Size: 402 152 5420
Fragments arranged by size:
5420 402 152
SalI G'TCGA_C
Cuts at: 1936 2992 3316 1936
Size: 1056 324 4594
Fragments arranged by size:
4594 1056 324
SapI GCTCTTCn'nnn_
Cuts at: 2774 2774
Size: 5974
SbfI CC_TGCA'GG
Cuts at: 3326 3326
Size: 5974
ScaI AGT'ACT
Cuts at: 4222 5934 4222
Size: 1712 4262
SfcI C'TryA_G
Cuts at: 826 1045 2587 3322 4457 5138 5329 826
Size: 219 1542 735 1135 681 191 1471
Fragments arranged by size:
1542 1471 1135 735 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5974
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5974
SmaI CCC'GGG
Cuts at: 1872 3301 1872
Size: 1429 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2525 2716 4078 4979 5241 820
Size: 197 926 582 191 1362 901 262 1553
Fragments arranged by size:
1553 1362 926 901 582 262 197 191
SnaBI TAC'GTA
Cuts at: 493 3765 493
Size: 3272 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5974
SphI G_CATG'C
Cuts at: 1132 3332 1132
Size: 2200 3774
SspI AAT'ATT
Cuts at: 5 52 2911 3898 5
Size: 47 2859 987 2081
Fragments arranged by size:
2859 2081 987 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5974
StyI C'CwwG_G
Cuts at: 513 1065 2004 3413 513
Size: 552 939 1409 3074
Fragments arranged by size:
3074 1409 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2094 2114 2516 2773 2869 4001 4018
Size: 1255 514 20 402 257 96 1132 17
Cuts at: 4018 4171 4356 4801 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1132 514 445 402 257 185
153 96 20 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2291 4220 5932
Size: 274 80 33 51 1443 314 1929 1712
Cuts at: 5932 96
Size: 138
Fragments arranged by size:
1929 1712 1443 314 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2801 2833 3202 3234 5934 5966 2801
Size: 32 369 32 2700 32 2809
Fragments arranged by size:
2809 2700 369 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5974
XbaI T'CTAG_A
Cuts at: 3310 3310
Size: 5974
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2158 2452 2158
Size: 294 5680
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5974
XmaI C'CCGG_G
Cuts at: 1870 3299 1870
Size: 1429 4545
XmnI GAAnn'nnTTC
Cuts at: 4103 4103
Size: 5974
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3782 276
Size: 53 83 186 936 2248 2468
Fragments arranged by size:
2468 2248 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PspXI PsrI RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI