Restriction map for
pFN21AE2187
(Circular) (Six-base) MAPSORT of: pFN21AE2187.seq Check: 5138 from: 1 to: 6127
pFN21AE2187 6127 bp
With 182 enzymes: SgfI *
August 16, 2010 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3937 278
Size: 53 83 186 936 2401 2468
Fragments arranged by size:
2468 2401 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2090 2962 3470 1937
Size: 153 872 508 4594
Fragments arranged by size:
4594 872 508 153
Acc65I G'GTAC_C
Cuts at: 3448 3448
Size: 6127
AclI AA'CG_TT
Cuts at: 1563 4254 4627 1563
Size: 2691 373 3063
Fragments arranged by size:
3063 2691 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2243 2270 2840 4190 5574 5842 1632
Size: 611 27 570 1350 1384 268 1917
Fragments arranged by size:
1917 1384 1350 611 570 268 27
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5930
AflIII A'CryG_T
Cuts at: 1203 5026 6003 1203
Size: 3823 977 1327
Fragments arranged by size:
3823 1327 977
AgeI A'CCGG_T
Cuts at: 4958 4958
Size: 6127
AhdI GACnn_n'nnGTC
Cuts at: 4856 4856
Size: 6127
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6127
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2279 1184
Size: 1095 5032
AlwNI CAG_nnn'CTG
Cuts at: 2875 3870 5442 2875
Size: 995 1572 3560
Fragments arranged by size:
3560 1572 995
ApaLI G'TGCA_C
Cuts at: 4184 5340 4184
Size: 1156 4971
ApoI r'AATT_y
Cuts at: 1493 2333 2361 2420 2507 3269 3436 3662 3814
Size: 840 28 59 87 762 167 226 152
Cuts at: 3814 5923 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 840 762 226 167 152 87
59 28
AseI AT'TA_AT
Cuts at: 160 1032 4681 160
Size: 872 3649 1606
Fragments arranged by size:
3649 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6127
AvaI C'yCGr_G
Cuts at: 1870 1943 2095 3190 3452 1870
Size: 73 152 1095 262 4545
Fragments arranged by size:
4545 1095 262 152 73
BamHI G'GATC_C
Cuts at: 3457 3824 3457
Size: 367 5760
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3448 4903 618
Size: 325 217 627 1661 1455 1842
Fragments arranged by size:
1842 1661 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2181 2439 3446 1391
Size: 790 258 1007 4072
Fragments arranged by size:
4072 1007 790 258
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3188 928
Size: 971 1289 3867
Fragments arranged by size:
3867 1289 971
BbvCI CC'TCA_GC
Cuts at: 3367 3367
Size: 6127
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3360 3394 4318 4352 1903
Size: 34 1423 34 924 34 3678
Fragments arranged by size:
3678 1423 924 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4019 5229 4019
Size: 1210 4917
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6127
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4738 136
Size: 107 122 71 631 605 3066 1525
Fragments arranged by size:
3066 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6122 6122
Size: 6127
BlpI GC'TnA_GC
Cuts at: 2319 3544 2319
Size: 1225 4902
Bme1580I G_kGCm'C
Cuts at: 1790 4188 5344 1790
Size: 2398 1156 2573
Fragments arranged by size:
2573 2398 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6127
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3308 3858 4816
Size: 416 53 83 399 24 1891 550 958
Cuts at: 4816 442
Size: 1753
Fragments arranged by size:
1891 1753 958 550 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6127
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2856 2972 2856
Size: 116 6011
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2192 2255 2425 2567 2751 2897 2900 4787
Size: 953 63 170 142 184 146 3 1887
Cuts at: 4787 1239
Size: 2579
Fragments arranged by size:
2579 1887 953 184 170 146 142 63
3
Bpu10I CC'TnA_GC
Cuts at: 3367 3367
Size: 6127
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2929 4252 5117 5415 2929
Size: 1323 865 298 3641
Fragments arranged by size:
3641 1323 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2231 4790 882
Size: 617 732 2559 2219
Fragments arranged by size:
2559 2219 732 617
BsaAI yAC'GTr
Cuts at: 493 1204 3202 3918 493
Size: 711 1998 716 2702
Fragments arranged by size:
2702 1998 716 711
BsaBI GATnn'nnATC
Cuts at: 6121 6121
Size: 6127
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3934 4316 4951 275
Size: 53 83 186 936 2401 382 635 1451
Fragments arranged by size:
2401 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4560 4958 5232 5379 5790 4560
Size: 398 274 147 411 4897
Fragments arranged by size:
4897 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3064 3094 3274 3304 3401
Size: 30 165 30 1661 30 180 30 97
Cuts at: 3401 3431 1178
Size: 30 3874
Fragments arranged by size:
3874 1661 180 165 97 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2189 2285 2894 3406 1186
Size: 1003 96 609 512 3907
Fragments arranged by size:
3907 1003 609 512 96
BseYI C'CCAG_C
Cuts at: 3420 5330 5826 3420
Size: 1910 496 3721
Fragments arranged by size:
3721 1910 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3012 3224 1163
Size: 573 1276 212 4066
Fragments arranged by size:
4066 1276 573 212
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2067 3577 4338 5366 1540
Size: 275 186 66 1510 761 1028 2301
Fragments arranged by size:
2301 1510 1028 761 275 186 66
BsiHKAI G_wGCw'C
Cuts at: 2181 2439 4188 4273 5344 2181
Size: 258 1749 85 1071 2964
Fragments arranged by size:
2964 1749 1071 258 85
BsmI GAATG_Cn'
Cuts at: 2851 3638 3731 2851
Size: 787 93 5247
Fragments arranged by size:
5247 787 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2584 2725 2584
Size: 141 5986
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2181 2439 3446 4188 4273 5344 1391
Size: 399 391 258 1007 742 85 1071 2174
Fragments arranged by size:
2174 1071 1007 742 399 391 258 85
BspEI T'CCGG_A
Cuts at: 5790 5790
Size: 6127
BspHI T'CATG_A
Cuts at: 4016 5746 4016
Size: 1730 4397
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2292 3482 844
Size: 1448 1190 3489
Fragments arranged by size:
3489 1448 1190
BsrBI CCG'CTC
Cuts at: 1394 1823 4014 1394
Size: 429 2191 3507
Fragments arranged by size:
3507 2191 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4622 4796 65
Size: 1183 3374 174 1396
Fragments arranged by size:
3374 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4771 4958 1700
Size: 110 2961 187 2869
Fragments arranged by size:
2961 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6127
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6127
BssSI C'ACGA_G
Cuts at: 4187 5199 4187
Size: 1012 5115
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6127
BstBI TT'CG_AA
Cuts at: 4931 4931
Size: 6127
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6127
BstXI CCAn_nnnn'nTGG
Cuts at: 2191 2191
Size: 6127
BstYI r'GATC_y
Cuts at: 1972 3457 3824 4209 4226 5678 5764 5776 6122
Size: 1485 367 385 17 1452 86 12 346
Cuts at: 6122 1972
Size: 1977
Fragments arranged by size:
1977 1485 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2751 3223 3245 3357 513
Size: 552 939 747 472 22 112 3283
Fragments arranged by size:
3283 939 747 552 472 112 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2718 487
Size: 683 56 1492 3896
Fragments arranged by size:
3896 1492 683 56
BtsI GCAGTG_nn'
Cuts at: 2261 3643 4437 4457 2261
Size: 1382 794 20 3931
Fragments arranged by size:
3931 1382 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6127
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6092
DraI TTT'AAA
Cuts at: 2799 3431 2799
Size: 632 5495
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2717 5134 809
Size: 507 1401 2417 1802
Fragments arranged by size:
2417 1802 1401 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2064 3574 4463
Size: 54 1425 50 275 18 234 1510 889
Cuts at: 4463 6092 8
Size: 1629 43
Fragments arranged by size:
1629 1510 1425 889 275 234 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2064 3574 1537
Size: 275 252 1510 4090
Fragments arranged by size:
4090 1510 275 252
EarI CTCTTCn'nnn_
Cuts at: 1353 2895 2941 4057 1353
Size: 1542 46 1116 3423
Fragments arranged by size:
3423 1542 1116 46
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 2156 3318 3330 4714 5088
Size: 1064 138 435 132 1162 12 1384 374
Cuts at: 5088 5234 387
Size: 146 1280
Fragments arranged by size:
1384 1280 1162 1064 435 374 146 138
132 12
EcoICRI GAG'CTC
Cuts at: 2179 2437 2179
Size: 258 5869
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2192 2243 2255 2270 2425 2567 2751
Size: 393 560 51 12 15 155 142 184
Cuts at: 2751 2840 2897 2900 4190 4787 5574 5842 1239
Size: 89 57 3 1290 597 787 268 1524
Fragments arranged by size:
1524 1290 787 597 560 393 268 184
155 142 89 57 51 15 12 3
EcoRI G'AATT_C
Cuts at: 2333 2420 3436 3814 2333
Size: 87 1016 378 4646
Fragments arranged by size:
4646 1016 378 87
EcoRV GAT'ATC
Cuts at: 2746 2746
Size: 6127
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2408 2408
Size: 6127
FspI TGC'GCA
Cuts at: 2288 4633 2288
Size: 2345 3782
HaeII r_GCGC'y
Cuts at: 1678 2019 5274 1678
Size: 341 3255 2531
Fragments arranged by size:
3255 2531 341
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2749 2781 2966 2998 4768
Size: 32 135 32 1177 32 185 32 1770
Cuts at: 4768 4800 4842 4874 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1770 1177 185 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2091 2518 2963 3471 3717 669
Size: 988 281 153 427 445 508 246 3079
Fragments arranged by size:
3079 988 508 445 427 281 246 153
HindIII A'AGCT_T
Cuts at: 748 2672 748
Size: 1924 4203
HpaI GTT'AAC
Cuts at: 3717 3717
Size: 6127
KpnI G_GTAC'C
Cuts at: 3452 3452
Size: 6127
MfeI C'AATT_G
Cuts at: 3726 3726
Size: 6127
MluI A'CGCG_T
Cuts at: 6003 6003
Size: 6127
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5241 5425 1305
Size: 3936 184 2007
Fragments arranged by size:
3936 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4305
Fragments arranged by size:
4305 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4085 4444 4603 518
Size: 648 2919 359 159 2042
Fragments arranged by size:
2919 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3543 4220 5368 5613 1606
Size: 1937 677 1148 245 2120
Fragments arranged by size:
2120 1937 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2751 3223 513
Size: 552 939 747 472 3417
Fragments arranged by size:
3417 939 747 552 472
NdeI CA'TA_TG
Cuts at: 387 3118 387
Size: 2731 3396
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6127
NotI GC'GGCC_GC
Cuts at: 3574 3574
Size: 6127
NspI r_CATG'y
Cuts at: 1132 1229 3485 5030 1132
Size: 97 2256 1545 2229
Fragments arranged by size:
2256 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6127
PciI A'CATG_T
Cuts at: 5026 5026
Size: 6127
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6127
PmeI GTTT'AAAC
Cuts at: 3431 3431
Size: 6127
PmlI CAC'GTG
Cuts at: 3202 3202
Size: 6127
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4196 4228 5709 5741 4196
Size: 32 1481 32 4582
Fragments arranged by size:
4582 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3314 1613
Size: 1701 4426
PsiI TTA'TAA
Cuts at: 3697 3697
Size: 6127
PstI C_TGCA'G
Cuts at: 830 2205 2268 2700 2931 3479 830
Size: 1375 63 432 231 548 3478
Fragments arranged by size:
3478 1375 548 432 231 63
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6127
SacI G_AGCT'C
Cuts at: 2181 2439 2181
Size: 258 5869
SalI G'TCGA_C
Cuts at: 1936 2089 2961 3469 1936
Size: 153 872 508 4594
Fragments arranged by size:
4594 872 508 153
SbfI CC_TGCA'GG
Cuts at: 2700 3479 2700
Size: 779 5348
ScaI AGT'ACT
Cuts at: 4375 6087 4375
Size: 1712 4415
SexAI A'CCwGG_T
Cuts at: 3106 3106
Size: 6127
SfcI C'TryA_G
Cuts at: 826 1045 2201 2264 2696 2927 3475 4610 5291
Size: 219 1156 63 432 231 548 1135 681
Cuts at: 5291 5482 826
Size: 191 1471
Fragments arranged by size:
1471 1156 1135 681 548 432 231 219
191 63
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6127
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6127
SmaI CCC'GGG
Cuts at: 1872 3192 3454 1872
Size: 1320 262 4545
Fragments arranged by size:
4545 1320 262
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2908 4231 5132 5394 820
Size: 197 926 965 1323 901 262 1553
Fragments arranged by size:
1553 1323 965 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3918 493
Size: 3425 2702
SpeI A'CTAG_T
Cuts at: 152 2641 152
Size: 2489 3638
SphI G_CATG'C
Cuts at: 1132 3485 1132
Size: 2353 3774
SspI AAT'ATT
Cuts at: 5 52 4051 5
Size: 47 3999 2081
Fragments arranged by size:
3999 2081 47
StuI AGG'CCT
Cuts at: 1517 3097 3238 1517
Size: 1580 141 4406
Fragments arranged by size:
4406 1580 141
StyI C'CwwG_G
Cuts at: 513 1065 2004 2751 3223 3566 513
Size: 552 939 747 472 343 3074
Fragments arranged by size:
3074 939 747 552 472 343
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3361 4154 4171 4324 4509 4954 325
Size: 1255 1781 793 17 153 185 445 1498
Fragments arranged by size:
1781 1498 1255 793 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4373 6085 96
Size: 274 80 33 51 1443 2396 1712 138
Fragments arranged by size:
2396 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3064 3096 3274 3306 3401 3433 6087 6119 3064
Size: 32 178 32 95 32 2654 32 3072
Fragments arranged by size:
3072 2654 178 95 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6127
XbaI T'CTAG_A
Cuts at: 3463 3463
Size: 6127
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2992 2992
Size: 6127
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6127
XmaI C'CCGG_G
Cuts at: 1870 3190 3452 1870
Size: 1320 262 4545
Fragments arranged by size:
4545 1320 262
XmnI GAAnn'nnTTC
Cuts at: 4256 4256
Size: 6127
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3935 276
Size: 53 83 186 936 2401 2468
Fragments arranged by size:
2468 2401 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PstI PvuI SacI SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoO109I FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII
SanDI SapI SfiI SfoI SrfI SwaI