Restriction map for
pFN21AE2186
(Circular) (Six-base) MAPSORT of: pFN21AE2186.seq Check: 639 from: 1 to: 6460
pFN21AE2186 6460 bp
With 182 enzymes: SgfI *
July 19, 2019 13:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3461 3730 3461
Size: 269 6191
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4270 278
Size: 53 83 186 936 2734 2468
Fragments arranged by size:
2734 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2970 3803 1937
Size: 1033 833 4594
Fragments arranged by size:
4594 1033 833
Acc65I G'GTAC_C
Cuts at: 2662 3781 2662
Size: 1119 5341
AclI AA'CG_TT
Cuts at: 1563 4587 4960 1563
Size: 3024 373 3063
Fragments arranged by size:
3063 3024 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2624 2668 4523 5907 6175 1632
Size: 992 44 1855 1384 268 1917
Fragments arranged by size:
1917 1855 1384 992 268 44
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6263
AflIII A'CryG_T
Cuts at: 1203 5359 6336 1203
Size: 4156 977 1327
Fragments arranged by size:
4156 1327 977
AgeI A'CCGG_T
Cuts at: 5291 5291
Size: 6460
AhdI GACnn_n'nnGTC
Cuts at: 5189 5189
Size: 6460
AleI CACnn'nnGTG
Cuts at: 1166 2762 1166
Size: 1596 4864
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6460
AlwNI CAG_nnn'CTG
Cuts at: 3193 3612 4203 5775 3193
Size: 419 591 1572 3878
Fragments arranged by size:
3878 1572 591 419
ApaI G_GGCC'C
Cuts at: 2564 2939 3224 3719 2564
Size: 375 285 495 5305
Fragments arranged by size:
5305 495 375 285
ApaLI G'TGCA_C
Cuts at: 2204 2947 4517 5673 2204
Size: 743 1570 1156 2991
Fragments arranged by size:
2991 1570 1156 743
ApoI r'AATT_y
Cuts at: 1493 3769 3995 4147 6256 1493
Size: 2276 226 152 2109 1697
Fragments arranged by size:
2276 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5014 160
Size: 872 3982 1606
Fragments arranged by size:
3982 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6460
AvaI C'yCGr_G
Cuts at: 1870 1943 2387 2536 3463 3497 3674 3785 1870
Size: 73 444 149 927 34 177 111 4545
Fragments arranged by size:
4545 927 444 177 149 111 73 34
BamHI G'GATC_C
Cuts at: 3790 4157 3790
Size: 367 6093
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2116 2476 2527 2647 2662
Size: 325 217 627 329 360 51 120 15
Cuts at: 2662 3314 3650 3781 5236 618
Size: 652 336 131 1455 1842
Fragments arranged by size:
1842 1455 652 627 360 336 329 325
217 131 120 51 15
BanII G_rGCy'C
Cuts at: 1391 2564 2589 2939 3143 3224 3719 3779 1391
Size: 1173 25 350 204 81 495 60 4072
Fragments arranged by size:
4072 1173 495 350 204 81 60 25
BbeI G_GCGC'C
Cuts at: 2480 2531 3654 2480
Size: 51 1123 5286
Fragments arranged by size:
5286 1123 51
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2950 928
Size: 971 1051 4438
Fragments arranged by size:
4438 1051 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4651 4685 1903
Size: 34 2714 34 3678
Fragments arranged by size:
3678 2714 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4352 5562 4352
Size: 1210 5250
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6460
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2373 2474 2485
Size: 107 122 71 631 605 701 101 11
Cuts at: 2485 2891 5071 136
Size: 406 2180 1525
Fragments arranged by size:
2180 1525 701 631 605 406 122 107
101 71 11
BglII A'GATC_T
Cuts at: 6455 6455
Size: 6460
BlpI GC'TnA_GC
Cuts at: 3137 3877 3137
Size: 740 5720
Bme1580I G_kGCm'C
Cuts at: 1790 2121 2208 2564 2598 2652 2939 2951 3224
Size: 331 87 356 34 54 287 12 273
Cuts at: 3224 3251 3719 4521 5677 1790
Size: 27 468 802 1156 2573
Fragments arranged by size:
2573 1156 802 468 356 331 287 273
87 54 34 27 12
BmgBI CAC'GTC
Cuts at: 1318 2754 3552 1318
Size: 1436 798 4226
Fragments arranged by size:
4226 1436 798
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3152 3514 4191
Size: 416 53 83 399 24 1735 362 677
Cuts at: 4191 5149 442
Size: 958 1753
Fragments arranged by size:
1753 1735 958 677 416 399 362 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6460
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2219 3353 5120 1239
Size: 980 1134 1767 2579
Fragments arranged by size:
2579 1767 1134 980
Bpu10I CC'TnA_GC
Cuts at: 2805 2805
Size: 6460
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3503 4585 5450 5748 3503
Size: 1082 865 298 4215
Fragments arranged by size:
4215 1082 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2921 5123 882
Size: 617 1422 2202 2219
Fragments arranged by size:
2219 2202 1422 617
BsaAI yAC'GTr
Cuts at: 493 1204 3178 3757 4251 493
Size: 711 1974 579 494 2702
Fragments arranged by size:
2702 1974 711 579 494
BsaBI GATnn'nnATC
Cuts at: 3350 6454 3350
Size: 3104 3356
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2430 2477 2528 3558
Size: 53 83 186 936 897 47 51 1030
Cuts at: 3558 3651 4267 4649 5284 275
Size: 93 616 382 635 1451
Fragments arranged by size:
1451 1030 936 897 635 616 382 186
93 83 53 51 47
BsaWI w'CCGG_w
Cuts at: 4893 5291 5565 5712 6123 4893
Size: 398 274 147 411 5230
Fragments arranged by size:
5230 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6235
Fragments arranged by size:
6235 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6460
BseYI C'CCAG_C
Cuts at: 2419 2494 2555 2851 3107 3217 3435 5663 6159
Size: 75 61 296 256 110 218 2228 496
Cuts at: 6159 2419
Size: 2720
Fragments arranged by size:
2720 2228 496 296 256 218 110 75
61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2925 1163
Size: 573 1189 4698
Fragments arranged by size:
4698 1189 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2079 2407 3910 4671 5699 1540
Size: 275 186 78 328 1503 761 1028 2301
Fragments arranged by size:
2301 1503 1028 761 328 275 186 78
BsiHKAI G_wGCw'C
Cuts at: 2208 2910 2951 3143 3234 3513 4521 4606 5677
Size: 702 41 192 91 279 1008 85 1071
Cuts at: 5677 2208
Size: 2991
Fragments arranged by size:
2991 1071 1008 702 279 192 91 85
41
BsmI GAATG_Cn'
Cuts at: 2552 3971 4064 2552
Size: 1419 93 4948
Fragments arranged by size:
4948 1419 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2121 2208 2564 2589 2598 2652 2910
Size: 399 331 87 356 25 9 54 258
Cuts at: 2910 2939 2951 3143 3224 3234 3251 3513 3719
Size: 29 12 192 81 10 17 262 206
Cuts at: 3719 3779 4521 4606 5677 1391
Size: 60 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 356 331 262 258
206 192 87 85 81 60 54 29
25 17 12 10 9
BspEI T'CCGG_A
Cuts at: 6123 6123
Size: 6460
BspHI T'CATG_A
Cuts at: 3342 4349 6079 3342
Size: 1007 1730 3723
Fragments arranged by size:
3723 1730 1007
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2256 3246 3461 3730 3815 844
Size: 1412 990 215 269 85 3489
Fragments arranged by size:
3489 1412 990 269 215 85
BsrBI CCG'CTC
Cuts at: 1394 1823 2322 2620 3645 4347 1394
Size: 429 499 298 1025 702 3507
Fragments arranged by size:
3507 1025 702 499 429 298
BsrDI GCAATG_nn'
Cuts at: 65 1248 4955 5129 65
Size: 1183 3707 174 1396
Fragments arranged by size:
3707 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2345 2530 3035 3164 3476 5104 5291
Size: 110 535 185 505 129 312 1628 187
Cuts at: 5291 1700
Size: 2869
Fragments arranged by size:
2869 1628 535 505 312 187 185 129
110
BsrGI T'GTAC_A
Cuts at: 96 2271 96
Size: 2175 4285
BssHII G'CGCG_C
Cuts at: 1925 2335 3487 1925
Size: 410 1152 4898
Fragments arranged by size:
4898 1152 410
BssSI C'ACGA_G
Cuts at: 4520 5532 4520
Size: 1012 5448
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2400 794
Size: 1606 4854
BstBI TT'CG_AA
Cuts at: 5264 5264
Size: 6460
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6460
BstXI CCAn_nnnn'nTGG
Cuts at: 2764 3394 2764
Size: 630 5830
BstYI r'GATC_y
Cuts at: 1972 2229 3564 3790 4157 4542 4559 6011 6097
Size: 257 1335 226 367 385 17 1452 86
Cuts at: 6097 6109 6455 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1335 385 367 346 257 226
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2128 2472 2931 3144 3626 3654
Size: 552 939 124 344 459 213 482 28
Cuts at: 3654 513
Size: 3319
Fragments arranged by size:
3319 939 552 482 459 344 213 124
28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3046 487
Size: 683 56 1820 3901
Fragments arranged by size:
3901 1820 683 56
BtsI GCAGTG_nn'
Cuts at: 3976 4770 4790 3976
Size: 794 20 5646
Fragments arranged by size:
5646 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6460
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6425
DraI TTT'AAA
Cuts at: 3764 3764
Size: 6460
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2435 3586 5467 809
Size: 507 1119 1151 1881 1802
Fragments arranged by size:
1881 1802 1151 1119 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2076 2216 2766
Size: 54 1425 50 275 18 246 140 550
Cuts at: 2766 2985 3030 3162 3478 3634 3657 3907 4796
Size: 219 45 132 316 156 23 250 889
Cuts at: 4796 6425 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 550 316 275 250 246
219 156 140 132 54 50 45 43
23 18
EagI C'GGCC_G
Cuts at: 1537 1812 2076 3907 1537
Size: 275 264 1831 4090
Fragments arranged by size:
4090 1831 275 264
EarI CTCTTCn'nnn_
Cuts at: 1353 3260 3287 4390 1353
Size: 1907 27 1103 3423
Fragments arranged by size:
3423 1907 1103 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5047 5421 5567 387
Size: 1064 138 3458 374 146 1280
Fragments arranged by size:
3458 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3141 3141
Size: 6460
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2219 2624 2668 3353 4523 5120 5907
Size: 393 587 405 44 685 1170 597 787
Cuts at: 5907 6175 1239
Size: 268 1524
Fragments arranged by size:
1524 1170 787 685 597 587 405 393
268 44
EcoO109I rG'GnC_Cy
Cuts at: 2092 2437 2560 2936 3154 3716 2092
Size: 345 123 376 218 562 4836
Fragments arranged by size:
4836 562 376 345 218 123
EcoRI G'AATT_C
Cuts at: 3769 4147 3769
Size: 378 6082
FspI TGC'GCA
Cuts at: 4966 4966
Size: 6460
HaeII r_GCGC'y
Cuts at: 1678 2480 2531 2812 3137 3449 3654 3687 5607
Size: 802 51 281 325 312 205 33 1920
Cuts at: 5607 1678
Size: 2531
Fragments arranged by size:
2531 1920 802 325 312 281 205 51
33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5101 5133 5175 5207 1373
Size: 32 135 32 3529 32 42 32 2626
Fragments arranged by size:
3529 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3804 4050 669
Size: 988 281 1866 246 3079
Fragments arranged by size:
3079 1866 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6460
HpaI GTT'AAC
Cuts at: 4050 4050
Size: 6460
KasI G'GCGC_C
Cuts at: 2476 2527 3650 2476
Size: 51 1123 5286
Fragments arranged by size:
5286 1123 51
KpnI G_GTAC'C
Cuts at: 2666 3785 2666
Size: 1119 5341
MfeI C'AATT_G
Cuts at: 4059 4059
Size: 6460
MluI A'CGCG_T
Cuts at: 6336 6336
Size: 6460
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5574 5758 1305
Size: 4269 184 2007
Fragments arranged by size:
4269 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2768 2987 10
Size: 54 1425 343 936 219 3483
Fragments arranged by size:
3483 1425 936 343 219 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2762 2783 3395 4418 4777 4936 518
Size: 648 1596 21 612 1023 359 159 2042
Fragments arranged by size:
2042 1596 1023 648 612 359 159 21
MspA1I CmG'CkG
Cuts at: 1606 2062 2130 2221 2308 2359 2555 3226 3612
Size: 456 68 91 87 51 196 671 386
Cuts at: 3612 3628 3876 4553 5701 5946 1606
Size: 16 248 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 671 456 386 248 245
196 91 87 68 51 16
NaeI GCC'GGC
Cuts at: 2347 2532 3037 3166 2347
Size: 185 505 129 5641
Fragments arranged by size:
5641 505 185 129
NarI GG'CG_CC
Cuts at: 2477 2528 3651 2477
Size: 51 1123 5286
Fragments arranged by size:
5286 1123 51
NcoI C'CATG_G
Cuts at: 513 1065 2004 3654 513
Size: 552 939 1650 3319
Fragments arranged by size:
3319 1650 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6460
NgoMIV G'CCGG_C
Cuts at: 2345 2530 3035 3164 2345
Size: 185 505 129 5641
Fragments arranged by size:
5641 505 185 129
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6460
NotI GC'GGCC_GC
Cuts at: 2076 3907 2076
Size: 1831 4629
NruI TCG'CGA
Cuts at: 2583 2583
Size: 6460
NspI r_CATG'y
Cuts at: 1132 1229 2158 3818 5363 1132
Size: 97 929 1660 1545 2229
Fragments arranged by size:
2229 1660 1545 929 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6460
PciI A'CATG_T
Cuts at: 5359 5359
Size: 6460
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2715 3000 1108
Size: 1607 285 4568
Fragments arranged by size:
4568 1607 285
PmeI GTTT'AAAC
Cuts at: 3764 3764
Size: 6460
PmlI CAC'GTG
Cuts at: 3757 3757
Size: 6460
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2687 2719 3182 3214 4529 4561 6042 6074 2687
Size: 32 463 32 1315 32 1481 32 3073
Fragments arranged by size:
3073 1481 1315 463 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2437 3154 2437
Size: 717 5743
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6460
PsiI TTA'TAA
Cuts at: 4030 4030
Size: 6460
PspOMI G'GGCC_C
Cuts at: 2560 2935 3220 3715 2560
Size: 375 285 495 5305
Fragments arranged by size:
5305 495 375 285
PstI C_TGCA'G
Cuts at: 830 2900 3812 830
Size: 2070 912 3478
Fragments arranged by size:
3478 2070 912
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6460
PvuII CAG'CTG
Cuts at: 2062 2062
Size: 6460
SacI G_AGCT'C
Cuts at: 3143 3143
Size: 6460
SacII CC_GC'GG
Cuts at: 2131 3629 2131
Size: 1498 4962
SalI G'TCGA_C
Cuts at: 1936 3802 1936
Size: 1866 4594
SanDI GG'GwC_CC
Cuts at: 3154 3154
Size: 6460
SapI GCTCTTCn'nnn_
Cuts at: 3260 3287 3260
Size: 27 6433
SbfI CC_TGCA'GG
Cuts at: 3812 3812
Size: 6460
ScaI AGT'ACT
Cuts at: 3080 3503 3524 4708 6420 3080
Size: 423 21 1184 1712 3120
Fragments arranged by size:
3120 1712 1184 423 21
SexAI A'CCwGG_T
Cuts at: 3020 3020
Size: 6460
SfcI C'TryA_G
Cuts at: 826 1045 2896 3808 4943 5624 5815 826
Size: 219 1851 912 1135 681 191 1471
Fragments arranged by size:
1851 1471 1135 912 681 219 191
SfoI GGC'GCC
Cuts at: 2478 2529 3652 2478
Size: 51 1123 5286
Fragments arranged by size:
5286 1123 51
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6460
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6460
SmaI CCC'GGG
Cuts at: 1872 3465 3787 1872
Size: 1593 322 4545
Fragments arranged by size:
4545 1593 322
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3518 4564 5465 5727 820
Size: 197 926 1575 1046 901 262 1553
Fragments arranged by size:
1575 1553 1046 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4251 493
Size: 3758 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6460
SphI G_CATG'C
Cuts at: 1132 3818 1132
Size: 2686 3774
SrfI GCCC'GGGC
Cuts at: 3465 3465
Size: 6460
SspI AAT'ATT
Cuts at: 5 52 4384 5
Size: 47 4332 2081
Fragments arranged by size:
4332 2081 47
StuI AGG'CCT
Cuts at: 1517 2488 2724 1517
Size: 971 236 5253
Fragments arranged by size:
5253 971 236
StyI C'CwwG_G
Cuts at: 513 1065 2004 2088 2340 3654 3899 513
Size: 552 939 84 252 1314 245 3074
Fragments arranged by size:
3074 1314 939 552 252 245 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3496 4487 4504 4657 4842 5287 325
Size: 1255 1916 991 17 153 185 445 1498
Fragments arranged by size:
1916 1498 1255 991 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2271 3078 3501
Size: 274 80 33 51 1443 294 807 423
Cuts at: 3501 3522 4706 6418 96
Size: 21 1184 1712 138
Fragments arranged by size:
1712 1443 1184 807 423 294 274 138
80 51 33 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6420 6452 6420
Size: 32 6428
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6460
XbaI T'CTAG_A
Cuts at: 3796 3796
Size: 6460
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3127 3394 3127
Size: 267 6193
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6460
XmaI C'CCGG_G
Cuts at: 1870 3463 3785 1870
Size: 1593 322 4545
Fragments arranged by size:
4545 1593 322
XmnI GAAnn'nnTTC
Cuts at: 4589 4589
Size: 6460
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4268 276
Size: 53 83 186 936 2734 2468
Fragments arranged by size:
2734 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI
FseI FspAI NsiI PacI PfoI PspXI PsrI RsrII
SfiI SwaI