Restriction map for
pFN21AE2183
(Circular) (Six-base) MAPSORT of: pFN21AE2183.seq Check: 4780 from: 1 to: 6496
pFN21AE2183 6496 bp
With 182 enzymes: SgfI *
July 6, 2012 17:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2899 2899
Size: 6496
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4306 278
Size: 53 83 186 936 2770 2468
Fragments arranged by size:
2770 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3839 1937
Size: 1902 4594
Acc65I G'GTAC_C
Cuts at: 3265 3817 3265
Size: 552 5944
AclI AA'CG_TT
Cuts at: 1563 4623 4996 1563
Size: 3060 373 3063
Fragments arranged by size:
3063 3060 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2282 3663 4559 5943 6211 1632
Size: 650 1381 896 1384 268 1917
Fragments arranged by size:
1917 1384 1381 896 650 268
AflII C'TTAA_G
Cuts at: 820 1017 2933 820
Size: 197 1916 4383
Fragments arranged by size:
4383 1916 197
AflIII A'CryG_T
Cuts at: 1203 5395 6372 1203
Size: 4192 977 1327
Fragments arranged by size:
4192 1327 977
AgeI A'CCGG_T
Cuts at: 5327 5327
Size: 6496
AhdI GACnn_n'nnGTC
Cuts at: 2185 5225 2185
Size: 3040 3456
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6496
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2120 1184
Size: 936 5560
AlwNI CAG_nnn'CTG
Cuts at: 3058 4239 5811 3058
Size: 1181 1572 3743
Fragments arranged by size:
3743 1572 1181
ApaLI G'TGCA_C
Cuts at: 4553 5709 4553
Size: 1156 5340
ApoI r'AATT_y
Cuts at: 1493 2209 2682 3136 3686 3805 4031 4183 6292
Size: 716 473 454 550 119 226 152 2109
Cuts at: 6292 1493
Size: 1697
Fragments arranged by size:
2109 1697 716 550 473 454 226 152
119
AseI AT'TA_AT
Cuts at: 160 1032 2964 3600 5050 160
Size: 872 1932 636 1450 1606
Fragments arranged by size:
1932 1606 1450 872 636
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6496
AvaI C'yCGr_G
Cuts at: 1870 1943 3821 1870
Size: 73 1878 4545
Fragments arranged by size:
4545 1878 73
BamHI G'GATC_C
Cuts at: 3826 4193 3826
Size: 367 6129
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3265 3817 5272 618
Size: 325 217 627 1478 552 1455 1842
Fragments arranged by size:
1842 1478 1455 627 552 325 217
BanII G_rGCy'C
Cuts at: 1391 3006 3097 3815 1391
Size: 1615 91 718 4072
Fragments arranged by size:
4072 1615 718 91
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3651 928
Size: 971 1752 3773
Fragments arranged by size:
3773 1752 971
BbvCI CC'TCA_GC
Cuts at: 2979 2979
Size: 6496
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4687 4721 1903
Size: 34 2750 34 3678
Fragments arranged by size:
3678 2750 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4388 5598 4388
Size: 1210 5286
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6496
BfrBI ATG'CAT
Cuts at: 2119 2119
Size: 6496
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5107 136
Size: 107 122 71 631 605 3435 1525
Fragments arranged by size:
3435 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2155 2164 2440 3148 6491 2155
Size: 9 276 708 3343 2160
Fragments arranged by size:
3343 2160 708 276 9
BlpI GC'TnA_GC
Cuts at: 3913 3913
Size: 6496
Bme1580I G_kGCm'C
Cuts at: 1790 4557 5713 1790
Size: 2767 1156 2573
Fragments arranged by size:
2767 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6496
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4227 5185 442
Size: 416 53 83 399 24 2810 958 1753
Fragments arranged by size:
2810 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6496
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3081 3635 5156 1239
Size: 1842 554 1521 2579
Fragments arranged by size:
2579 1842 1521 554
Bpu10I CC'TnA_GC
Cuts at: 2979 2979
Size: 6496
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2786 4621 5486 5784 2786
Size: 1835 865 298 3498
Fragments arranged by size:
3498 1835 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5159 882
Size: 617 3660 2219
Fragments arranged by size:
3660 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4287 493
Size: 711 3083 2702
Fragments arranged by size:
3083 2702 711
BsaBI GATnn'nnATC
Cuts at: 6490 6490
Size: 6496
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4303 4685 5320 275
Size: 53 83 186 936 2770 382 635 1451
Fragments arranged by size:
2770 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2011 4929 5327 5601 5748 6159 2011
Size: 2918 398 274 147 411 2348
Fragments arranged by size:
2918 2348 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6271
Fragments arranged by size:
6271 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6496
BseYI C'CCAG_C
Cuts at: 3005 3694 5699 6195 3005
Size: 689 2005 496 3306
Fragments arranged by size:
3306 2005 689 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5923
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3946 4707 5735 1540
Size: 275 186 1945 761 1028 2301
Fragments arranged by size:
2301 1945 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3097 4557 4642 5713 3097
Size: 1460 85 1071 3880
Fragments arranged by size:
3880 1460 1071 85
BsmI GAATG_Cn'
Cuts at: 4007 4100 4007
Size: 93 6403
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3006 3097 3815 4557 4642 5713 1391
Size: 399 1216 91 718 742 85 1071 2174
Fragments arranged by size:
2174 1216 1071 742 718 399 91 85
BspEI T'CCGG_A
Cuts at: 6159 6159
Size: 6496
BspHI T'CATG_A
Cuts at: 2058 4385 6115 2058
Size: 2327 1730 2439
Fragments arranged by size:
2439 2327 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2899 3610 3619 3772 3851 844
Size: 2055 711 9 153 79 3489
Fragments arranged by size:
3489 2055 711 153 79 9
BsrBI CCG'CTC
Cuts at: 1394 1823 4383 1394
Size: 429 2560 3507
Fragments arranged by size:
3507 2560 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4991 5165 65
Size: 1183 3743 174 1396
Fragments arranged by size:
3743 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5140 5327 1700
Size: 110 3330 187 2869
Fragments arranged by size:
3330 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6496
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6496
BssSI C'ACGA_G
Cuts at: 3680 4556 5568 3680
Size: 876 1012 4608
Fragments arranged by size:
4608 1012 876
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3625 794
Size: 2831 3665
BstBI TT'CG_AA
Cuts at: 5300 5300
Size: 6496
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6496
BstYI r'GATC_y
Cuts at: 1972 2155 2164 2440 3148 3826 4193 4578 4595
Size: 183 9 276 708 678 367 385 17
Cuts at: 4595 6047 6133 6145 6491 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 708 678 385 367 346 276
183 86 17 12 9
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5944
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5757
Fragments arranged by size:
5757 683 56
BtsI GCAGTG_nn'
Cuts at: 4012 4806 4826 4012
Size: 794 20 5682
Fragments arranged by size:
5682 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6496
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6461
DraI TTT'AAA
Cuts at: 3450 3800 3450
Size: 350 6146
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5503 809
Size: 507 4187 1802
Fragments arranged by size:
4187 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3943 4832 6461
Size: 54 1425 50 275 18 2113 889 1629
Cuts at: 6461 8
Size: 43
Fragments arranged by size:
2113 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3943 1537
Size: 275 2131 4090
Fragments arranged by size:
4090 2131 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4426 1353
Size: 3073 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5083 5457 5603 387
Size: 1064 138 3494 374 146 1280
Fragments arranged by size:
3494 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3095 3095
Size: 6496
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2282 3081 3635 3663 4559 5156 5943
Size: 393 650 799 554 28 896 597 787
Cuts at: 5943 6211 1239
Size: 268 1524
Fragments arranged by size:
1524 896 799 787 650 597 554 393
268 28
EcoO109I rG'GnC_Cy
Cuts at: 3028 3638 3028
Size: 610 5886
EcoRI G'AATT_C
Cuts at: 3805 4183 3805
Size: 378 6118
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2430 2430
Size: 6496
FspI TGC'GCA
Cuts at: 5002 5002
Size: 6496
HaeII r_GCGC'y
Cuts at: 1678 5643 1678
Size: 3965 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2171 2203 2384 2416 3533
Size: 32 135 32 599 32 181 32 1117
Cuts at: 3533 3565 5137 5169 5211 5243 1373
Size: 32 1572 32 42 32 2626
Fragments arranged by size:
2626 1572 1117 599 181 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2042 2243 3840 4086 669
Size: 988 281 104 201 1597 246 3079
Fragments arranged by size:
3079 1597 988 281 246 201 104
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6496
HpaI GTT'AAC
Cuts at: 4086 4086
Size: 6496
KpnI G_GTAC'C
Cuts at: 3269 3821 3269
Size: 552 5944
MfeI C'AATT_G
Cuts at: 4095 4095
Size: 6496
MluI A'CGCG_T
Cuts at: 6372 6372
Size: 6496
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3559 5610 5794 1305
Size: 2254 2051 184 2007
Fragments arranged by size:
2254 2051 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4674
Fragments arranged by size:
4674 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2102 4454 4813 4972 518
Size: 648 936 2352 359 159 2042
Fragments arranged by size:
2352 2042 936 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3912 4589 5737 5982 1606
Size: 2306 677 1148 245 2120
Fragments arranged by size:
2306 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5944
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6496
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6496
NotI GC'GGCC_GC
Cuts at: 3943 3943
Size: 6496
NsiI A_TGCA'T
Cuts at: 2121 2121
Size: 6496
NspI r_CATG'y
Cuts at: 1132 1229 3854 5399 1132
Size: 97 2625 1545 2229
Fragments arranged by size:
2625 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6496
PciI A'CATG_T
Cuts at: 5395 5395
Size: 6496
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6496
PmeI GTTT'AAAC
Cuts at: 3800 3800
Size: 6496
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4565 4597 6078 6110 4565
Size: 32 1481 32 4951
Fragments arranged by size:
4951 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3638 3638
Size: 6496
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6496
PsiI TTA'TAA
Cuts at: 2487 2769 2901 3111 4066 2487
Size: 282 132 210 955 4917
Fragments arranged by size:
4917 955 282 210 132
PstI C_TGCA'G
Cuts at: 830 3259 3769 3848 830
Size: 2429 510 79 3478
Fragments arranged by size:
3478 2429 510 79
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6496
SacI G_AGCT'C
Cuts at: 3097 3097
Size: 6496
SalI G'TCGA_C
Cuts at: 1936 3838 1936
Size: 1902 4594
SbfI CC_TGCA'GG
Cuts at: 3848 3848
Size: 6496
ScaI AGT'ACT
Cuts at: 3786 4744 6456 3786
Size: 958 1712 3826
Fragments arranged by size:
3826 1712 958
SfcI C'TryA_G
Cuts at: 826 1045 2542 2740 3255 3589 3765 3844 4979
Size: 219 1497 198 515 334 176 79 1135
Cuts at: 4979 5660 5851 826
Size: 681 191 1471
Fragments arranged by size:
1497 1471 1135 681 515 334 219 198
191 176 79
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6496
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6496
SmaI CCC'GGG
Cuts at: 1872 3823 1872
Size: 1951 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2801 2933 4600 5501 5763 820
Size: 197 926 858 132 1667 901 262 1553
Fragments arranged by size:
1667 1553 926 901 858 262 197 132
SnaBI TAC'GTA
Cuts at: 493 4287 493
Size: 3794 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6496
SphI G_CATG'C
Cuts at: 1132 3854 1132
Size: 2722 3774
SspI AAT'ATT
Cuts at: 5 52 4420 5
Size: 47 4368 2081
Fragments arranged by size:
4368 2081 47
StuI AGG'CCT
Cuts at: 1517 2775 2875 1517
Size: 1258 100 5138
Fragments arranged by size:
5138 1258 100
StyI C'CwwG_G
Cuts at: 513 1065 3269 3935 513
Size: 552 2204 666 3074
Fragments arranged by size:
3074 2204 666 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4523 4540 4693 4878 5323 325
Size: 1255 2943 17 153 185 445 1498
Fragments arranged by size:
2943 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3784 4742 6454
Size: 274 80 33 51 1443 1807 958 1712
Cuts at: 6454 96
Size: 138
Fragments arranged by size:
1807 1712 1443 958 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6456 6488 6456
Size: 32 6464
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6496
XbaI T'CTAG_A
Cuts at: 3832 3832
Size: 6496
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6496
XmaI C'CCGG_G
Cuts at: 1870 3821 1870
Size: 1951 4545
XmnI GAAnn'nnTTC
Cuts at: 4625 4625
Size: 6496
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4304 276
Size: 53 83 186 936 2770 2468
Fragments arranged by size:
2770 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI