Restriction map for
pFN21AE2182
(Circular) (Six-base) MAPSORT of: pFN21AE2182.seq Check: 9938 from: 1 to: 5248
pFN21AE2182 5248 bp
With 182 enzymes: SgfI *
July 6, 2012 17:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2138 2138
Size: 5248
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3058 278
Size: 53 83 186 936 1522 2468
Fragments arranged by size:
2468 1522 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2054 2591 1937
Size: 117 537 4594
Fragments arranged by size:
4594 537 117
Acc65I G'GTAC_C
Cuts at: 2569 2569
Size: 5248
AclI AA'CG_TT
Cuts at: 1563 3375 3748 1563
Size: 1812 373 3063
Fragments arranged by size:
3063 1812 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3311 4695 4963 1632
Size: 1679 1384 268 1917
Fragments arranged by size:
1917 1679 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5051
AflIII A'CryG_T
Cuts at: 1203 4147 5124 1203
Size: 2944 977 1327
Fragments arranged by size:
2944 1327 977
AgeI A'CCGG_T
Cuts at: 4079 4079
Size: 5248
AhdI GACnn_n'nnGTC
Cuts at: 3977 3977
Size: 5248
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5248
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5248
AlwNI CAG_nnn'CTG
Cuts at: 2991 4563 2991
Size: 1572 3676
ApaLI G'TGCA_C
Cuts at: 3305 4461 3305
Size: 1156 4092
ApoI r'AATT_y
Cuts at: 1493 2557 2783 2935 5044 1493
Size: 1064 226 152 2109 1697
Fragments arranged by size:
2109 1697 1064 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3802 160
Size: 872 2770 1606
Fragments arranged by size:
2770 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5248
AvaI C'yCGr_G
Cuts at: 1870 1943 2573 1870
Size: 73 630 4545
Fragments arranged by size:
4545 630 73
BamHI G'GATC_C
Cuts at: 2578 2945 2578
Size: 367 4881
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2569 4024 618
Size: 325 217 627 782 1455 1842
Fragments arranged by size:
1842 1455 782 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2244 2567 1391
Size: 853 323 4072
Fragments arranged by size:
4072 853 323
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2405 928
Size: 971 506 3771
Fragments arranged by size:
3771 971 506
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3439 3473 1903
Size: 34 50 34 1418 34 3678
Fragments arranged by size:
3678 1418 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3140 4350 3140
Size: 1210 4038
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5248
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2037 2432 3859
Size: 107 122 71 631 605 365 395 1427
Cuts at: 3859 136
Size: 1525
Fragments arranged by size:
1525 1427 631 605 395 365 122 107
71
BglII A'GATC_T
Cuts at: 5243 5243
Size: 5248
BlpI GC'TnA_GC
Cuts at: 2665 2665
Size: 5248
Bme1580I G_kGCm'C
Cuts at: 1790 3309 4465 1790
Size: 1519 1156 2573
Fragments arranged by size:
2573 1519 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5248
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2979 3937 442
Size: 416 53 83 399 24 1562 958 1753
Fragments arranged by size:
1753 1562 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5248
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3908 1239
Size: 2669 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3373 4238 4536 3373
Size: 865 298 4085
Fragments arranged by size:
4085 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3911 882
Size: 617 2412 2219
Fragments arranged by size:
2412 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3039 493
Size: 711 1835 2702
Fragments arranged by size:
2702 1835 711
BsaBI GATnn'nnATC
Cuts at: 5242 5242
Size: 5248
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3055 3437 4072 275
Size: 53 83 186 936 1522 382 635 1451
Fragments arranged by size:
1522 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2442 3681 4079 4353 4500 4911 2442
Size: 1239 398 274 147 411 2779
Fragments arranged by size:
2779 1239 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5023
Fragments arranged by size:
5023 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2081 2132 2344 2471 1186
Size: 895 51 212 127 3963
Fragments arranged by size:
3963 895 212 127 51
BseYI C'CCAG_C
Cuts at: 4451 4947 4451
Size: 496 4752
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4675
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2698 3459 4487 1540
Size: 275 186 697 761 1028 2301
Fragments arranged by size:
2301 1028 761 697 275 186
BsiHKAI G_wGCw'C
Cuts at: 3309 3394 4465 3309
Size: 85 1071 4092
Fragments arranged by size:
4092 1071 85
BsmI GAATG_Cn'
Cuts at: 2529 2759 2852 2529
Size: 230 93 4925
Fragments arranged by size:
4925 230 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2244 2567 3309 3394 4465 1391
Size: 399 454 323 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 454 399 323 85
BspEI T'CCGG_A
Cuts at: 2442 4911 2442
Size: 2469 2779
BspHI T'CATG_A
Cuts at: 3137 4867 3137
Size: 1730 3518
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2138 2603 844
Size: 1294 465 3489
Fragments arranged by size:
3489 1294 465
BsrBI CCG'CTC
Cuts at: 1394 1823 3135 1394
Size: 429 1312 3507
Fragments arranged by size:
3507 1312 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3743 3917 65
Size: 1183 2495 174 1396
Fragments arranged by size:
2495 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3892 4079 1700
Size: 110 2082 187 2869
Fragments arranged by size:
2869 2082 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5248
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5248
BssSI C'ACGA_G
Cuts at: 3308 4320 3308
Size: 1012 4236
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5248
BstBI TT'CG_AA
Cuts at: 4052 4052
Size: 5248
BstEII G'GTnAC_C
Cuts at: 1184 2150 1184
Size: 966 4282
BstXI CCAn_nnnn'nTGG
Cuts at: 2269 2269
Size: 5248
BstYI r'GATC_y
Cuts at: 1972 2302 2578 2945 3330 3347 4799 4885 4897
Size: 330 276 367 385 17 1452 86 12
Cuts at: 4897 5243 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 346 330 276 86
17 12
BstZ17I GTA'TAC
Cuts at: 2055 2055
Size: 5248
Bsu36I CC'TnA_GG
Cuts at: 2064 2064
Size: 5248
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4696
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4509
Fragments arranged by size:
4509 683 56
BtsI GCAGTG_nn'
Cuts at: 2764 3558 3578 2764
Size: 794 20 4434
Fragments arranged by size:
4434 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5248
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5213
DraI TTT'AAA
Cuts at: 2552 2552
Size: 5248
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4255 809
Size: 507 2939 1802
Fragments arranged by size:
2939 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2391 2433 2695
Size: 54 1425 50 275 18 561 42 262
Cuts at: 2695 3584 5213 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 561 275 262 54 50
43 42 18
EagI C'GGCC_G
Cuts at: 1537 1812 2695 1537
Size: 275 883 4090
Fragments arranged by size:
4090 883 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2109 2337 2472 3178 1353
Size: 756 228 135 706 3423
Fragments arranged by size:
3423 756 706 228 135
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3835 4209 4355 387
Size: 1064 138 2246 374 146 1280
Fragments arranged by size:
2246 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3311 3908 4695 4963 1239
Size: 393 1679 597 787 268 1524
Fragments arranged by size:
1679 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2013 2543 2013
Size: 530 4718
EcoRI G'AATT_C
Cuts at: 2557 2935 2557
Size: 378 4870
FspI TGC'GCA
Cuts at: 3754 3754
Size: 5248
HaeII r_GCGC'y
Cuts at: 1678 4395 1678
Size: 2717 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2156 2188 3889 3921 3963
Size: 32 135 32 584 32 1701 32 42
Cuts at: 3963 3995 1373
Size: 32 2626
Fragments arranged by size:
2626 1701 584 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2592 2838 669
Size: 988 281 654 246 3079
Fragments arranged by size:
3079 988 654 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5248
HpaI GTT'AAC
Cuts at: 2838 2838
Size: 5248
KpnI G_GTAC'C
Cuts at: 2573 2573
Size: 5248
MfeI C'AATT_G
Cuts at: 2847 2847
Size: 5248
MluI A'CGCG_T
Cuts at: 5124 5124
Size: 5248
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4362 4546 1305
Size: 3057 184 2007
Fragments arranged by size:
3057 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2435 10
Size: 54 1425 343 603 2823
Fragments arranged by size:
2823 1425 603 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2381 3206 3565 3724 518
Size: 648 1215 825 359 159 2042
Fragments arranged by size:
2042 1215 825 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2083 2452 2664 3341 4489 4734 1606
Size: 477 369 212 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 477 369 245 212
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4696
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5248
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5248
NotI GC'GGCC_GC
Cuts at: 2695 2695
Size: 5248
NspI r_CATG'y
Cuts at: 1132 1229 2606 4151 1132
Size: 97 1377 1545 2229
Fragments arranged by size:
2229 1545 1377 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5248
PciI A'CATG_T
Cuts at: 4147 4147
Size: 5248
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5248
PmeI GTTT'AAAC
Cuts at: 2552 2552
Size: 5248
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3317 3349 4830 4862 3317
Size: 32 1481 32 3703
Fragments arranged by size:
3703 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5248
PsiI TTA'TAA
Cuts at: 2818 2818
Size: 5248
PstI C_TGCA'G
Cuts at: 830 2088 2149 2600 830
Size: 1258 61 451 3478
Fragments arranged by size:
3478 1258 451 61
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5248
PvuII CAG'CTG
Cuts at: 2083 2083
Size: 5248
SalI G'TCGA_C
Cuts at: 1936 2590 1936
Size: 654 4594
SbfI CC_TGCA'GG
Cuts at: 2600 2600
Size: 5248
ScaI AGT'ACT
Cuts at: 3496 5208 3496
Size: 1712 3536
SfcI C'TryA_G
Cuts at: 826 1045 2084 2145 2596 3731 4412 4603 826
Size: 219 1039 61 451 1135 681 191 1471
Fragments arranged by size:
1471 1135 1039 681 451 219 191 61
SfiI GGCCn_nnn'nGGCC
Cuts at: 2037 2432 2037
Size: 395 4853
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5248
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5248
SmaI CCC'GGG
Cuts at: 1872 2575 1872
Size: 703 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3352 4253 4515 820
Size: 197 926 1409 901 262 1553
Fragments arranged by size:
1553 1409 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3039 493
Size: 2546 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5248
SphI G_CATG'C
Cuts at: 1132 2606 1132
Size: 1474 3774
SspI AAT'ATT
Cuts at: 5 52 3172 5
Size: 47 3120 2081
Fragments arranged by size:
3120 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5248
StyI C'CwwG_G
Cuts at: 513 1065 2412 2687 513
Size: 552 1347 275 3074
Fragments arranged by size:
3074 1347 552 275
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3275 3292 3445 3630 4075 325
Size: 1255 1695 17 153 185 445 1498
Fragments arranged by size:
1695 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3494 5206 96
Size: 274 80 33 51 1443 1517 1712 138
Fragments arranged by size:
1712 1517 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5208 5240 5208
Size: 32 5216
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5248
XbaI T'CTAG_A
Cuts at: 2176 2584 2176
Size: 408 4840
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2419 2419
Size: 5248
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5248
XmaI C'CCGG_G
Cuts at: 1870 2573 1870
Size: 703 4545
XmnI GAAnn'nnTTC
Cuts at: 3377 3377
Size: 5248
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3056 276
Size: 53 83 186 936 1522 2468
Fragments arranged by size:
2468 1522 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI DraIII EcoICRI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfoI
SrfI SwaI