Restriction map for
pFN21AE2181
(Circular) (Six-base) MAPSORT of: pFN21AE2181.seq Check: 1609 from: 1 to: 5425
pFN21AE2181 5425 bp
With 182 enzymes: SgfI *
July 6, 2012 17:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3235 278
Size: 53 83 186 936 1699 2468
Fragments arranged by size:
2468 1699 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2768 1937
Size: 831 4594
Acc65I G'GTAC_C
Cuts at: 2746 2746
Size: 5425
AclI AA'CG_TT
Cuts at: 1563 2227 3552 3925 1563
Size: 664 1325 373 3063
Fragments arranged by size:
3063 1325 664 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3488 4872 5140 1632
Size: 1856 1384 268 1917
Fragments arranged by size:
1917 1856 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2132 820
Size: 197 1115 4113
Fragments arranged by size:
4113 1115 197
AflIII A'CryG_T
Cuts at: 1203 4324 5301 1203
Size: 3121 977 1327
Fragments arranged by size:
3121 1327 977
AgeI A'CCGG_T
Cuts at: 4256 4256
Size: 5425
AhdI GACnn_n'nnGTC
Cuts at: 4154 4154
Size: 5425
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5425
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5425
AlwNI CAG_nnn'CTG
Cuts at: 3168 4740 3168
Size: 1572 3853
ApaLI G'TGCA_C
Cuts at: 3482 4638 3482
Size: 1156 4269
ApoI r'AATT_y
Cuts at: 1493 2624 2734 2960 3112 5221 1493
Size: 1131 110 226 152 2109 1697
Fragments arranged by size:
2109 1697 1131 226 152 110
AseI AT'TA_AT
Cuts at: 160 1032 3979 160
Size: 872 2947 1606
Fragments arranged by size:
2947 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5425
AvaI C'yCGr_G
Cuts at: 1870 1943 2096 2750 1870
Size: 73 153 654 4545
Fragments arranged by size:
4545 654 153 73
BamHI G'GATC_C
Cuts at: 2755 3122 2755
Size: 367 5058
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2070 2465 2746 4201 618
Size: 325 217 627 283 395 281 1455 1842
Fragments arranged by size:
1842 1455 627 395 325 283 281 217
BanII G_rGCy'C
Cuts at: 1391 2047 2744 1391
Size: 656 697 4072
Fragments arranged by size:
4072 697 656
BbeI G_GCGC'C
Cuts at: 2074 2074
Size: 5425
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2669 928
Size: 971 770 3684
Fragments arranged by size:
3684 971 770
BbvCI CC'TCA_GC
Cuts at: 2009 2102 2009
Size: 93 5332
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3616 3650 1903
Size: 34 50 34 1595 34 3678
Fragments arranged by size:
3678 1595 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3317 4527 3317
Size: 1210 4215
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5425
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4036 136
Size: 107 122 71 631 605 2364 1525
Fragments arranged by size:
2364 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5420 5420
Size: 5425
BlpI GC'TnA_GC
Cuts at: 2842 2842
Size: 5425
Bme1580I G_kGCm'C
Cuts at: 1790 3486 4642 1790
Size: 1696 1156 2573
Fragments arranged by size:
2573 1696 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5425
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3156 4114 442
Size: 416 53 83 399 24 1739 958 1753
Fragments arranged by size:
1753 1739 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5425
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4085 1239
Size: 2846 2579
Bpu10I CC'TnA_GC
Cuts at: 2009 2102 2009
Size: 93 5332
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3550 4415 4713 3550
Size: 865 298 4262
Fragments arranged by size:
4262 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4088 882
Size: 617 2589 2219
Fragments arranged by size:
2589 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2374 3216 493
Size: 711 1170 842 2702
Fragments arranged by size:
2702 1170 842 711
BsaBI GATnn'nnATC
Cuts at: 5419 5419
Size: 5425
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2071 3232 3614 4249
Size: 53 83 186 936 538 1161 382 635
Cuts at: 4249 275
Size: 1451
Fragments arranged by size:
1451 1161 936 635 538 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3858 4256 4530 4677 5088 3858
Size: 398 274 147 411 4195
Fragments arranged by size:
4195 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5200
Fragments arranged by size:
5200 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2026 1186
Size: 840 4585
BseYI C'CCAG_C
Cuts at: 2038 2156 2328 2516 4628 5124 2038
Size: 118 172 188 2112 496 2339
Fragments arranged by size:
2339 2112 496 188 172 118
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4852
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2057 2875 3636 4664 1540
Size: 275 186 56 818 761 1028 2301
Fragments arranged by size:
2301 1028 818 761 275 186 56
BsiHKAI G_wGCw'C
Cuts at: 3486 3571 4642 3486
Size: 85 1071 4269
Fragments arranged by size:
4269 1071 85
BsmI GAATG_Cn'
Cuts at: 2936 3029 2936
Size: 93 5332
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2047 2744 3486 3571 4642 1391
Size: 399 257 697 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 697 399 257 85
BspEI T'CCGG_A
Cuts at: 5088 5088
Size: 5425
BspHI T'CATG_A
Cuts at: 2529 3314 5044 2529
Size: 785 1730 2910
Fragments arranged by size:
2910 1730 785
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2780 844
Size: 1936 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3312 1394
Size: 429 1489 3507
Fragments arranged by size:
3507 1489 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2469 2514 3920 4094 65
Size: 1183 1221 45 1406 174 1396
Fragments arranged by size:
1406 1396 1221 1183 174 45
BsrFI r'CCGG_y
Cuts at: 1700 1810 2052 2067 4069 4256 1700
Size: 110 242 15 2002 187 2869
Fragments arranged by size:
2869 2002 242 187 110 15
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5425
BssHII G'CGCG_C
Cuts at: 1925 2024 1925
Size: 99 5326
BssSI C'ACGA_G
Cuts at: 3485 4497 3485
Size: 1012 4413
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5425
BstBI TT'CG_AA
Cuts at: 4229 4229
Size: 5425
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5425
BstYI r'GATC_y
Cuts at: 1972 2755 3122 3507 3524 4976 5062 5074 5420
Size: 783 367 385 17 1452 86 12 346
Cuts at: 5420 1972
Size: 1977
Fragments arranged by size:
1977 1452 783 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4873
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4686
Fragments arranged by size:
4686 683 56
BtsI GCAGTG_nn'
Cuts at: 2941 3735 3755 2941
Size: 794 20 4611
Fragments arranged by size:
4611 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5425
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5390
DraI TTT'AAA
Cuts at: 2729 2729
Size: 5425
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2144 4432 809
Size: 507 828 2288 1802
Fragments arranged by size:
2288 1802 828 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2054 2872 3761
Size: 54 1425 50 275 18 224 818 889
Cuts at: 3761 5390 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 818 275 224 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2054 2872 1537
Size: 275 242 818 4090
Fragments arranged by size:
4090 818 275 242
EarI CTCTTCn'nnn_
Cuts at: 1353 2339 3355 1353
Size: 986 1016 3423
Fragments arranged by size:
3423 1016 986
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4012 4386 4532 387
Size: 1064 138 2423 374 146 1280
Fragments arranged by size:
2423 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3488 4085 4872 5140 1239
Size: 393 1856 597 787 268 1524
Fragments arranged by size:
1856 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2170 2426 2170
Size: 256 5169
EcoRI G'AATT_C
Cuts at: 2734 3112 2734
Size: 378 5047
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2147 2147
Size: 5425
FspI TGC'GCA
Cuts at: 3931 3931
Size: 5425
HaeII r_GCGC'y
Cuts at: 1678 2074 4572 1678
Size: 396 2498 2531
Fragments arranged by size:
2531 2498 396
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2090 2122 2524 2556 4066
Size: 32 135 32 518 32 402 32 1510
Cuts at: 4066 4098 4140 4172 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1510 518 402 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2769 3015 669
Size: 988 281 831 246 3079
Fragments arranged by size:
3079 988 831 281 246
HindIII A'AGCT_T
Cuts at: 748 2297 2344 2686 748
Size: 1549 47 342 3487
Fragments arranged by size:
3487 1549 342 47
HpaI GTT'AAC
Cuts at: 3015 3015
Size: 5425
KasI G'GCGC_C
Cuts at: 2070 2070
Size: 5425
KpnI G_GTAC'C
Cuts at: 2750 2750
Size: 5425
MfeI C'AATT_G
Cuts at: 3024 3024
Size: 5425
MluI A'CGCG_T
Cuts at: 5301 5301
Size: 5425
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4539 4723 1305
Size: 3234 184 2007
Fragments arranged by size:
3234 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3603
Fragments arranged by size:
3603 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2463 3383 3742 3901 518
Size: 648 1297 920 359 159 2042
Fragments arranged by size:
2042 1297 920 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2160 2841 3518 4666 4911 1606
Size: 554 681 677 1148 245 2120
Fragments arranged by size:
2120 1148 681 677 554 245
NaeI GCC'GGC
Cuts at: 2054 2069 2054
Size: 15 5410
NarI GG'CG_CC
Cuts at: 2071 2071
Size: 5425
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4873
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5425
NgoMIV G'CCGG_C
Cuts at: 2052 2067 2052
Size: 15 5410
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5425
NotI GC'GGCC_GC
Cuts at: 2872 2872
Size: 5425
NspI r_CATG'y
Cuts at: 1132 1229 2783 4328 1132
Size: 97 1554 1545 2229
Fragments arranged by size:
2229 1554 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5425
PciI A'CATG_T
Cuts at: 4324 4324
Size: 5425
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5425
PfoI T'CCnGG_A
Cuts at: 2219 2219
Size: 5425
PmeI GTTT'AAAC
Cuts at: 2729 2729
Size: 5425
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3494 3526 5007 5039 3494
Size: 32 1481 32 3880
Fragments arranged by size:
3880 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2170 2170
Size: 5425
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5425
PsiI TTA'TAA
Cuts at: 2556 2696 2995 2556
Size: 140 299 4986
Fragments arranged by size:
4986 299 140
PstI C_TGCA'G
Cuts at: 830 2777 830
Size: 1947 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5425
PvuII CAG'CTG
Cuts at: 2160 2160
Size: 5425
SalI G'TCGA_C
Cuts at: 1936 2767 1936
Size: 831 4594
SapI GCTCTTCn'nnn_
Cuts at: 2339 2339
Size: 5425
SbfI CC_TGCA'GG
Cuts at: 2777 2777
Size: 5425
ScaI AGT'ACT
Cuts at: 3673 5385 3673
Size: 1712 3713
SfcI C'TryA_G
Cuts at: 826 1045 2773 3908 4589 4780 826
Size: 219 1728 1135 681 191 1471
Fragments arranged by size:
1728 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2072 2072
Size: 5425
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5425
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5425
SmaI CCC'GGG
Cuts at: 1872 2752 1872
Size: 880 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2132 3529 4430 4692 820
Size: 197 926 189 1397 901 262 1553
Fragments arranged by size:
1553 1397 926 901 262 197 189
SnaBI TAC'GTA
Cuts at: 493 3216 493
Size: 2723 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5425
SphI G_CATG'C
Cuts at: 1132 2783 1132
Size: 1651 3774
SspI AAT'ATT
Cuts at: 5 52 3349 5
Size: 47 3297 2081
Fragments arranged by size:
3297 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5425
StyI C'CwwG_G
Cuts at: 513 1065 2864 513
Size: 552 1799 3074
Fragments arranged by size:
3074 1799 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3452 3469 3622 3807 4252 325
Size: 1255 1872 17 153 185 445 1498
Fragments arranged by size:
1872 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3671 5383 96
Size: 274 80 33 51 1443 1694 1712 138
Fragments arranged by size:
1712 1694 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5385 5417 5385
Size: 32 5393
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5425
XbaI T'CTAG_A
Cuts at: 2761 2761
Size: 5425
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5425
XmaI C'CCGG_G
Cuts at: 1870 2750 1870
Size: 880 4545
XmnI GAAnn'nnTTC
Cuts at: 2127 2262 3554 2127
Size: 135 1292 3998
Fragments arranged by size:
3998 1292 135
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3233 276
Size: 53 83 186 936 1699 2468
Fragments arranged by size:
2468 1699 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FseI FspAI NruI NsiI PacI PmlI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI
SfiI SrfI SwaI XcmI