Restriction map for
pFN21AE2180
(Circular) (Six-base) MAPSORT of: pFN21AE2180.seq Check: 930 from: 1 to: 5956
pFN21AE2180 5956 bp
With 182 enzymes: SgfI *
March 28, 2012 13:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2827 2827
Size: 5956
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3766 278
Size: 53 83 186 936 2230 2468
Fragments arranged by size:
2468 2230 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3015 3299 1937
Size: 1078 284 4594
Fragments arranged by size:
4594 1078 284
Acc65I G'GTAC_C
Cuts at: 3277 3277
Size: 5956
AclI AA'CG_TT
Cuts at: 1563 4083 4456 1563
Size: 2520 373 3063
Fragments arranged by size:
3063 2520 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2356 2486 4019 5403 5671 1632
Size: 724 130 1533 1384 268 1917
Fragments arranged by size:
1917 1533 1384 724 268 130
AfeI AGC'GCT
Cuts at: 2067 2315 2395 2672 3176 2067
Size: 248 80 277 504 4847
Fragments arranged by size:
4847 504 277 248 80
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5759
AflIII A'CryG_T
Cuts at: 1203 4855 5832 1203
Size: 3652 977 1327
Fragments arranged by size:
3652 1327 977
AgeI A'CCGG_T
Cuts at: 4787 4787
Size: 5956
AhdI GACnn_n'nnGTC
Cuts at: 4685 4685
Size: 5956
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5956
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5956
AlwNI CAG_nnn'CTG
Cuts at: 2420 3249 3699 5271 2420
Size: 829 450 1572 3105
Fragments arranged by size:
3105 1572 829 450
ApaI G_GGCC'C
Cuts at: 2945 2945
Size: 5956
ApaLI G'TGCA_C
Cuts at: 2429 4013 5169 2429
Size: 1584 1156 3216
Fragments arranged by size:
3216 1584 1156
ApoI r'AATT_y
Cuts at: 1493 3265 3491 3643 5752 1493
Size: 1772 226 152 2109 1697
Fragments arranged by size:
2109 1772 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4510 160
Size: 872 3478 1606
Fragments arranged by size:
3478 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5956
AvaI C'yCGr_G
Cuts at: 1870 1943 2697 2846 2978 3281 1870
Size: 73 754 149 132 303 4545
Fragments arranged by size:
4545 754 303 149 132 73
BamHI G'GATC_C
Cuts at: 3286 3653 3286
Size: 367 5589
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2953 3071 3277 4732 618
Size: 325 217 627 1166 118 206 1455 1842
Fragments arranged by size:
1842 1455 1166 627 325 217 206 118
BanII G_rGCy'C
Cuts at: 1391 2163 2945 3275 1391
Size: 772 782 330 4072
Fragments arranged by size:
4072 782 772 330
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4985
BbvCI CC'TCA_GC
Cuts at: 2254 2254
Size: 5956
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2257 2291 4147 4181 1903
Size: 34 320 34 1856 34 3678
Fragments arranged by size:
3678 1856 320 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3848 5058 3848
Size: 1210 4746
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5956
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4567 136
Size: 107 122 71 631 605 2895 1525
Fragments arranged by size:
2895 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5951 5951
Size: 5956
BlpI GC'TnA_GC
Cuts at: 3373 3373
Size: 5956
Bme1580I G_kGCm'C
Cuts at: 1790 2433 2455 2945 2956 3074 4017 5173 1790
Size: 643 22 490 11 118 943 1156 2573
Fragments arranged by size:
2573 1156 943 643 490 118 22 11
BmgBI CAC'GTC
Cuts at: 1318 2805 2829 1318
Size: 1487 24 4445
Fragments arranged by size:
4445 1487 24
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3687 4645 442
Size: 416 53 83 399 24 2270 958 1753
Fragments arranged by size:
2270 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5956
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2354 2954 4616 1239
Size: 1115 600 1662 2579
Fragments arranged by size:
2579 1662 1115 600
Bpu10I CC'TnA_GC
Cuts at: 2204 2254 2567 2204
Size: 50 313 5593
Fragments arranged by size:
5593 313 50
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2311 2807 4081 4946 5244 2311
Size: 496 1274 865 298 3023
Fragments arranged by size:
3023 1274 865 496 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4619 882
Size: 617 3120 2219
Fragments arranged by size:
3120 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3747 493
Size: 711 2543 2702
Fragments arranged by size:
2702 2543 711
BsaBI GATnn'nnATC
Cuts at: 2279 5950 2279
Size: 3671 2285
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2843 3763 4145 4780
Size: 53 83 186 936 1310 920 382 635
Cuts at: 4780 275
Size: 1451
Fragments arranged by size:
1451 1310 936 920 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4389 4787 5061 5208 5619 4389
Size: 398 274 147 411 4726
Fragments arranged by size:
4726 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5731
Fragments arranged by size:
5731 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2519 1186
Size: 1333 4623
BseYI C'CCAG_C
Cuts at: 2046 2052 2590 2944 5159 5655 2046
Size: 6 538 354 2215 496 2347
Fragments arranged by size:
2347 2215 538 496 354 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2510 1163
Size: 573 774 4609
Fragments arranged by size:
4609 774 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2909 3139 3406 4167 5195 1540
Size: 275 186 908 230 267 761 1028 2301
Fragments arranged by size:
2301 1028 908 761 275 267 230 186
BsiHKAI G_wGCw'C
Cuts at: 2079 2169 2433 4017 4102 5173 2079
Size: 90 264 1584 85 1071 2862
Fragments arranged by size:
2862 1584 1071 264 90 85
BsmI GAATG_Cn'
Cuts at: 2294 3467 3560 2294
Size: 1173 93 4690
Fragments arranged by size:
4690 1173 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2079 2163 2169 2433 2455 2945 2956
Size: 399 289 84 6 264 22 490 11
Cuts at: 2956 3074 3275 4017 4102 5173 1391
Size: 118 201 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 490 399 289 264 201
118 85 84 22 11 6
BspEI T'CCGG_A
Cuts at: 5619 5619
Size: 5956
BspHI T'CATG_A
Cuts at: 3845 5575 3845
Size: 1730 4226
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2827 3311 844
Size: 1983 484 3489
Fragments arranged by size:
3489 1983 484
BsrBI CCG'CTC
Cuts at: 1394 1823 3843 1394
Size: 429 2020 3507
Fragments arranged by size:
3507 2020 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2192 4451 4625 65
Size: 1183 944 2259 174 1396
Fragments arranged by size:
2259 1396 1183 944 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2208 2962 3104 4600 4787 1700
Size: 110 398 754 142 1496 187 2869
Fragments arranged by size:
2869 1496 754 398 187 142 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5956
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5956
BssSI C'ACGA_G
Cuts at: 4016 5028 4016
Size: 1012 4944
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3129 3132 794
Size: 2335 3 3618
Fragments arranged by size:
3618 2335 3
BstBI TT'CG_AA
Cuts at: 3021 4760 3021
Size: 1739 4217
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5956
BstXI CCAn_nnnn'nTGG
Cuts at: 2070 2070
Size: 5956
BstYI r'GATC_y
Cuts at: 1972 2349 3286 3653 4038 4055 5507 5593 5605
Size: 377 937 367 385 17 1452 86 12
Cuts at: 5605 5951 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 937 385 377 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2057 2572 2797 2859 3075 3139
Size: 552 939 53 515 225 62 216 64
Cuts at: 3139 513
Size: 3330
Fragments arranged by size:
3330 939 552 515 225 216 64 62
53
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2395 487
Size: 683 56 1169 4048
Fragments arranged by size:
4048 1169 683 56
BtsI GCAGTG_nn'
Cuts at: 2546 2769 3181 3472 4266 4286 2546
Size: 223 412 291 794 20 4216
Fragments arranged by size:
4216 794 412 291 223 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5956
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5921
DraI TTT'AAA
Cuts at: 3260 3260
Size: 5956
DraIII CAC_nnn'GTG
Cuts at: 2184 2437 3237 2184
Size: 253 800 4903
Fragments arranged by size:
4903 800 253
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4963 809
Size: 507 3647 1802
Fragments arranged by size:
3647 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2060 2906 2926
Size: 54 1425 50 275 18 230 846 20
Cuts at: 2926 3136 3142 3403 4292 5921 8
Size: 210 6 261 889 1629 43
Fragments arranged by size:
1629 1425 889 846 275 261 230 210
54 50 43 20 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 2906 3136 3403 1537
Size: 275 1094 230 267 4090
Fragments arranged by size:
4090 1094 275 267 230
EarI CTCTTCn'nnn_
Cuts at: 1353 3213 3886 1353
Size: 1860 673 3423
Fragments arranged by size:
3423 1860 673
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2135 2797 4543 4917 5063 387
Size: 1064 138 546 662 1746 374 146 1280
Fragments arranged by size:
1746 1280 1064 662 546 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2354 2356 2486 2954 4019 4616 5403
Size: 393 722 2 130 468 1065 597 787
Cuts at: 5403 5671 1239
Size: 268 1524
Fragments arranged by size:
1524 1065 787 722 597 468 393 268
130 2
EcoNI CCTnn'n_nnAGG
Cuts at: 2515 2515
Size: 5956
EcoO109I rG'GnC_Cy
Cuts at: 2563 2578 2563
Size: 15 5941
EcoRI G'AATT_C
Cuts at: 3265 3643 3265
Size: 378 5578
FspI TGC'GCA
Cuts at: 2876 4462 2876
Size: 1586 4370
HaeII r_GCGC'y
Cuts at: 1678 2016 2069 2317 2397 2674 2870 2976 3105
Size: 338 53 248 80 277 196 106 129
Cuts at: 3105 3178 5103 1678
Size: 73 1925 2531
Fragments arranged by size:
2531 1925 338 277 248 196 129 106
80 73 53
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4597 4629 4671 4703 1373
Size: 32 135 32 3025 32 42 32 2626
Fragments arranged by size:
3025 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3300 3546 669
Size: 988 281 1362 246 3079
Fragments arranged by size:
3079 1362 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5956
HpaI GTT'AAC
Cuts at: 3546 3546
Size: 5956
KpnI G_GTAC'C
Cuts at: 3281 3281
Size: 5956
MfeI C'AATT_G
Cuts at: 3555 3555
Size: 5956
MluI A'CGCG_T
Cuts at: 5832 5832
Size: 5956
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2065 5070 5254 1305
Size: 760 3005 184 2007
Fragments arranged by size:
3005 2007 760 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4134
Fragments arranged by size:
4134 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2241 3914 4273 4432 518
Size: 648 1075 1673 359 159 2042
Fragments arranged by size:
2042 1673 1075 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2059 2420 2814 2932 2948 3141 3372 4049
Size: 453 361 394 118 16 193 231 677
Cuts at: 4049 5197 5442 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 453 394 361 245 231
193 118 16
NaeI GCC'GGC
Cuts at: 2210 2964 3106 2210
Size: 754 142 5060
Fragments arranged by size:
5060 754 142
NcoI C'CATG_G
Cuts at: 513 1065 2004 2572 513
Size: 552 939 568 3897
Fragments arranged by size:
3897 939 568 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5956
NgoMIV G'CCGG_C
Cuts at: 2208 2962 3104 2208
Size: 754 142 5060
Fragments arranged by size:
5060 754 142
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5956
NotI GC'GGCC_GC
Cuts at: 3136 3403 3136
Size: 267 5689
NspI r_CATG'y
Cuts at: 1132 1229 2779 3130 3314 4859 1132
Size: 97 1550 351 184 1545 2229
Fragments arranged by size:
2229 1550 1545 351 184 97
PasI CC'CwG_GG
Cuts at: 1348 2455 1348
Size: 1107 4849
PciI A'CATG_T
Cuts at: 4855 4855
Size: 5956
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5956
PfoI T'CCnGG_A
Cuts at: 2580 2580
Size: 5956
PmeI GTTT'AAAC
Cuts at: 3260 3260
Size: 5956
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4025 4057 5538 5570 4025
Size: 32 1481 32 4411
Fragments arranged by size:
4411 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2578 2578
Size: 5956
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5956
PsiI TTA'TAA
Cuts at: 3526 3526
Size: 5956
PspOMI G'GGCC_C
Cuts at: 2941 2941
Size: 5956
PstI C_TGCA'G
Cuts at: 830 2553 2622 2648 3308 830
Size: 1723 69 26 660 3478
Fragments arranged by size:
3478 1723 660 69 26
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5956
PvuII CAG'CTG
Cuts at: 2420 2814 2932 2420
Size: 394 118 5444
Fragments arranged by size:
5444 394 118
SacII CC_GC'GG
Cuts at: 2060 3142 2060
Size: 1082 4874
SalI G'TCGA_C
Cuts at: 1936 3298 1936
Size: 1362 4594
SanDI GG'GwC_CC
Cuts at: 2578 2578
Size: 5956
SbfI CC_TGCA'GG
Cuts at: 2622 3308 2622
Size: 686 5270
ScaI AGT'ACT
Cuts at: 4204 5916 4204
Size: 1712 4244
SexAI A'CCwGG_T
Cuts at: 3009 3009
Size: 5956
SfcI C'TryA_G
Cuts at: 826 1045 2549 2618 2644 3304 4439 5120 5311
Size: 219 1504 69 26 660 1135 681 191
Cuts at: 5311 826
Size: 1471
Fragments arranged by size:
1504 1471 1135 681 660 219 191 69
26
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5956
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5956
SmaI CCC'GGG
Cuts at: 1872 2699 2980 3283 1872
Size: 827 281 303 4545
Fragments arranged by size:
4545 827 303 281
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2326 2822 4060 4961 5223 820
Size: 197 926 383 496 1238 901 262 1553
Fragments arranged by size:
1553 1238 926 901 496 383 262 197
SnaBI TAC'GTA
Cuts at: 493 3747 493
Size: 3254 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5956
SphI G_CATG'C
Cuts at: 1132 3314 1132
Size: 2182 3774
SspI AAT'ATT
Cuts at: 5 52 3880 5
Size: 47 3828 2081
Fragments arranged by size:
3828 2081 47
StuI AGG'CCT
Cuts at: 1517 2202 2471 2510 2616 1517
Size: 685 269 39 106 4857
Fragments arranged by size:
4857 685 269 106 39
StyI C'CwwG_G
Cuts at: 513 1065 2004 2572 3395 513
Size: 552 939 568 823 3074
Fragments arranged by size:
3074 939 823 568 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2062 2140 2604 3983 4000 4153 4338
Size: 1255 482 78 464 1379 17 153 185
Cuts at: 4338 4783 325
Size: 445 1498
Fragments arranged by size:
1498 1379 1255 482 464 445 185 153
78 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4202 5914 96
Size: 274 80 33 51 1443 2225 1712 138
Fragments arranged by size:
2225 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2423 2455 5916 5948 2423
Size: 32 3461 32 2431
Fragments arranged by size:
3461 2431 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3011 1316
Size: 1695 4261
XbaI T'CTAG_A
Cuts at: 3292 3292
Size: 5956
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2134 2134
Size: 5956
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5956
XmaI C'CCGG_G
Cuts at: 1870 2697 2978 3281 1870
Size: 827 281 303 4545
Fragments arranged by size:
4545 827 303 281
XmnI GAAnn'nnTTC
Cuts at: 4085 4085
Size: 5956
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3764 276
Size: 53 83 186 936 2230 2468
Fragments arranged by size:
2468 2230 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacII SalI SanDI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI
KasI NarI NruI NsiI PacI PmlI PspXI PsrI
RsrII SacI SapI SfiI SfoI SrfI SwaI