Restriction map for
pFN21AE2174
(Circular) (Six-base) MAPSORT of: pFN21AE2174.seq Check: 5856 from: 1 to: 5545
pFN21AE2174 5545 bp
With 182 enzymes: SgfI *
August 16, 2010 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3355 278
Size: 53 83 186 936 1819 2468
Fragments arranged by size:
2468 1819 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2888 1937
Size: 951 4594
Acc65I G'GTAC_C
Cuts at: 2866 2866
Size: 5545
AclI AA'CG_TT
Cuts at: 1563 3672 4045 1563
Size: 2109 373 3063
Fragments arranged by size:
3063 2109 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3608 4992 5260 1632
Size: 1976 1384 268 1917
Fragments arranged by size:
1976 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5348
AflIII A'CryG_T
Cuts at: 1203 4444 5421 1203
Size: 3241 977 1327
Fragments arranged by size:
3241 1327 977
AgeI A'CCGG_T
Cuts at: 4376 4376
Size: 5545
AhdI GACnn_n'nnGTC
Cuts at: 2401 4274 2401
Size: 1873 3672
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5545
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2831 1184
Size: 1647 3898
AlwNI CAG_nnn'CTG
Cuts at: 3288 4860 3288
Size: 1572 3973
ApaI G_GGCC'C
Cuts at: 2346 2346
Size: 5545
ApaLI G'TGCA_C
Cuts at: 3602 4758 3602
Size: 1156 4389
ApoI r'AATT_y
Cuts at: 1493 2288 2854 3080 3232 5341 1493
Size: 795 566 226 152 2109 1697
Fragments arranged by size:
2109 1697 795 566 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4099 160
Size: 872 3067 1606
Fragments arranged by size:
3067 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5545
AvaI C'yCGr_G
Cuts at: 1870 1943 2514 2684 2735 2870 1870
Size: 73 571 170 51 135 4545
Fragments arranged by size:
4545 571 170 135 73 51
BamHI G'GATC_C
Cuts at: 2875 3242 2875
Size: 367 5178
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2080 2274 2576 2866 4321
Size: 325 217 627 293 194 302 290 1455
Cuts at: 4321 618
Size: 1842
Fragments arranged by size:
1842 1455 627 325 302 293 290 217
194
BanII G_rGCy'C
Cuts at: 1391 2120 2346 2864 1391
Size: 729 226 518 4072
Fragments arranged by size:
4072 729 518 226
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4574
BbvCI CC'TCA_GC
Cuts at: 2420 2420
Size: 5545
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3736 3770 1903
Size: 34 1799 34 3678
Fragments arranged by size:
3678 1799 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3437 4647 3437
Size: 1210 4335
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5545
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4156 136
Size: 107 122 71 631 605 2484 1525
Fragments arranged by size:
2484 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2058 2100 5540 2058
Size: 42 3440 2063
Fragments arranged by size:
3440 2063 42
BlpI GC'TnA_GC
Cuts at: 2962 2962
Size: 5545
Bme1580I G_kGCm'C
Cuts at: 1790 2346 2579 3606 4762 1790
Size: 556 233 1027 1156 2573
Fragments arranged by size:
2573 1156 1027 556 233
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5545
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3276 4234 442
Size: 416 53 83 399 24 1859 958 1753
Fragments arranged by size:
1859 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5545
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2139 2236 2139
Size: 97 5448
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2231 2270 4205 1239
Size: 992 39 1935 2579
Fragments arranged by size:
2579 1935 992 39
Bpu10I CC'TnA_GC
Cuts at: 2420 2420
Size: 5545
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2576 2765 2783 3670 4535 4833 2576
Size: 189 18 887 865 298 3288
Fragments arranged by size:
3288 887 865 298 189 18
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2042 4208 882
Size: 617 543 2166 2219
Fragments arranged by size:
2219 2166 617 543
BsaAI yAC'GTr
Cuts at: 493 1204 3336 493
Size: 711 2132 2702
Fragments arranged by size:
2702 2132 711
BsaBI GATnn'nnATC
Cuts at: 5539 5539
Size: 5545
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2538 3352 3734 4369
Size: 53 83 186 936 1005 814 382 635
Cuts at: 4369 275
Size: 1451
Fragments arranged by size:
1451 1005 936 814 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2013 2146 3978 4376 4650 4797 5208 2013
Size: 133 1832 398 274 147 411 2350
Fragments arranged by size:
2350 1832 411 398 274 147 133
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2141 2171 1178
Size: 30 165 30 738 30 4552
Fragments arranged by size:
4552 738 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2153 2404 2470 1186
Size: 967 251 66 4261
Fragments arranged by size:
4261 967 251 66
BseYI C'CCAG_C
Cuts at: 2193 2378 4748 5244 2193
Size: 185 2370 496 2494
Fragments arranged by size:
2494 2370 496 185
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2183 2810 1163
Size: 573 447 627 3898
Fragments arranged by size:
3898 627 573 447
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2995 3756 4784 1540
Size: 275 186 994 761 1028 2301
Fragments arranged by size:
2301 1028 994 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2619 3606 3691 4762 2619
Size: 987 85 1071 3402
Fragments arranged by size:
3402 1071 987 85
BsmI GAATG_Cn'
Cuts at: 3056 3149 3056
Size: 93 5452
BsmBI CGTCTCn'nnnn_
Cuts at: 2768 2768
Size: 5545
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2120 2346 2579 2619 2864 3606 3691
Size: 399 330 226 233 40 245 742 85
Cuts at: 3691 4762 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 330 245 233 226
85 40
BspEI T'CCGG_A
Cuts at: 2146 5208 2146
Size: 3062 2483
BspHI T'CATG_A
Cuts at: 3434 5164 3434
Size: 1730 3815
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2900 844
Size: 2056 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3432 1394
Size: 429 1609 3507
Fragments arranged by size:
3507 1609 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4040 4214 65
Size: 1183 2792 174 1396
Fragments arranged by size:
2792 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2556 4189 4376 1700
Size: 110 746 1633 187 2869
Fragments arranged by size:
2869 1633 746 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5545
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5545
BssSI C'ACGA_G
Cuts at: 3605 4617 3605
Size: 1012 4533
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5545
BstBI TT'CG_AA
Cuts at: 4349 4349
Size: 5545
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5545
BstXI CCAn_nnnn'nTGG
Cuts at: 2353 2353
Size: 5545
BstYI r'GATC_y
Cuts at: 1972 2058 2100 2568 2875 3242 3627 3644 5096
Size: 86 42 468 307 367 385 17 1452
Cuts at: 5096 5182 5194 5540 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 468 385 367 346 307 86
86 42 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2368 2640 513
Size: 552 1303 272 3418
Fragments arranged by size:
3418 1303 552 272
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2644 487
Size: 683 56 1418 3388
Fragments arranged by size:
3388 1418 683 56
BtsI GCAGTG_nn'
Cuts at: 2323 3061 3855 3875 2323
Size: 738 794 20 3993
Fragments arranged by size:
3993 794 738 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5545
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5510
DraI TTT'AAA
Cuts at: 2849 2849
Size: 5545
DraIII CAC_nnn'GTG
Cuts at: 2626 2626
Size: 5545
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4552 809
Size: 507 3236 1802
Fragments arranged by size:
3236 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2694 2992 3881
Size: 54 1425 50 275 18 864 298 889
Cuts at: 3881 5510 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 864 298 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2992 1537
Size: 275 1180 4090
Fragments arranged by size:
4090 1180 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2250 2302 2798 3475 1353
Size: 897 52 496 677 3423
Fragments arranged by size:
3423 897 677 496 52
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2044 2152 2365 4132 4506 4652
Size: 1064 138 455 108 213 1767 374 146
Cuts at: 4652 387
Size: 1280
Fragments arranged by size:
1767 1280 1064 455 374 213 146 138
108
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2231 2270 3608 4205 4992 5260 1239
Size: 393 599 39 1338 597 787 268 1524
Fragments arranged by size:
1524 1338 787 599 597 393 268 39
EcoO109I rG'GnC_Cy
Cuts at: 2245 2548 2245
Size: 303 5242
EcoRI G'AATT_C
Cuts at: 2854 3232 2854
Size: 378 5167
FspI TGC'GCA
Cuts at: 4051 4051
Size: 5545
HaeII r_GCGC'y
Cuts at: 1678 2448 2497 2648 2731 2798 4692 1678
Size: 770 49 151 83 67 1894 2531
Fragments arranged by size:
2531 1894 770 151 83 67 49
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2141 2173 2387 2419 4186
Size: 32 135 32 569 32 214 32 1767
Cuts at: 4186 4218 4260 4292 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1767 569 214 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2889 3135 669
Size: 988 281 951 246 3079
Fragments arranged by size:
3079 988 951 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5545
HpaI GTT'AAC
Cuts at: 3135 3135
Size: 5545
KpnI G_GTAC'C
Cuts at: 2870 2870
Size: 5545
MfeI C'AATT_G
Cuts at: 3144 3144
Size: 5545
MluI A'CGCG_T
Cuts at: 5421 5421
Size: 5545
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2003 2468 4659 4843 1305
Size: 698 465 2191 184 2007
Fragments arranged by size:
2191 2007 698 465 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3723
Fragments arranged by size:
3723 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3503 3862 4021 518
Size: 648 2337 359 159 2042
Fragments arranged by size:
2337 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2197 2281 2424 2751 2961 3638 4786 5031
Size: 591 84 143 327 210 677 1148 245
Cuts at: 5031 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 591 327 245 210 143
84
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4993
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5545
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5545
NotI GC'GGCC_GC
Cuts at: 2992 2992
Size: 5545
NspI r_CATG'y
Cuts at: 1132 1229 2602 2903 4448 1132
Size: 97 1373 301 1545 2229
Fragments arranged by size:
2229 1545 1373 301 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5545
PciI A'CATG_T
Cuts at: 4444 4444
Size: 5545
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2626 1108
Size: 1518 4027
PmeI GTTT'AAAC
Cuts at: 2849 2849
Size: 5545
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3614 3646 5127 5159 3614
Size: 32 1481 32 4000
Fragments arranged by size:
4000 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2245 2548 2245
Size: 303 5242
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5545
PsiI TTA'TAA
Cuts at: 3115 3115
Size: 5545
PspOMI G'GGCC_C
Cuts at: 2342 2342
Size: 5545
PspXI vC'TCGA_Gb
Cuts at: 2735 2735
Size: 5545
PstI C_TGCA'G
Cuts at: 830 2232 2897 830
Size: 1402 665 3478
Fragments arranged by size:
3478 1402 665
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5545
PvuII CAG'CTG
Cuts at: 2197 2281 2197
Size: 84 5461
SalI G'TCGA_C
Cuts at: 1936 2887 1936
Size: 951 4594
SapI GCTCTTCn'nnn_
Cuts at: 2798 2798
Size: 5545
SbfI CC_TGCA'GG
Cuts at: 2897 2897
Size: 5545
ScaI AGT'ACT
Cuts at: 2741 3793 5505 2741
Size: 1052 1712 2781
Fragments arranged by size:
2781 1712 1052
SfcI C'TryA_G
Cuts at: 826 1045 2228 2893 4028 4709 4900 826
Size: 219 1183 665 1135 681 191 1471
Fragments arranged by size:
1471 1183 1135 681 665 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5545
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5545
SmaI CCC'GGG
Cuts at: 1872 2516 2872 1872
Size: 644 356 4545
Fragments arranged by size:
4545 644 356
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2591 2735 2780 2798 3649 4550
Size: 197 926 648 144 45 18 851 901
Cuts at: 4550 4812 820
Size: 262 1553
Fragments arranged by size:
1553 926 901 851 648 262 197 144
45 18
SnaBI TAC'GTA
Cuts at: 493 3336 493
Size: 2843 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5545
SphI G_CATG'C
Cuts at: 1132 2903 1132
Size: 1771 3774
SspI AAT'ATT
Cuts at: 5 52 3469 5
Size: 47 3417 2081
Fragments arranged by size:
3417 2081 47
StuI AGG'CCT
Cuts at: 1517 2390 1517
Size: 873 4672
StyI C'CwwG_G
Cuts at: 513 1065 2508 2721 2984 513
Size: 552 1443 213 263 3074
Fragments arranged by size:
3074 1443 552 263 213
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3572 3589 3742 3927 4372 325
Size: 1255 1992 17 153 185 445 1498
Fragments arranged by size:
1992 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2739 3791 5503
Size: 274 80 33 51 1443 762 1052 1712
Cuts at: 5503 96
Size: 138
Fragments arranged by size:
1712 1443 1052 762 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5505 5537 5505
Size: 32 5513
Tth111I GACn'n_nGTC
Cuts at: 1316 2546 1316
Size: 1230 4315
XbaI T'CTAG_A
Cuts at: 2881 2881
Size: 5545
XhoI C'TCGA_G
Cuts at: 1943 2735 1943
Size: 792 4753
XmaI C'CCGG_G
Cuts at: 1870 2514 2870 1870
Size: 644 356 4545
Fragments arranged by size:
4545 644 356
XmnI GAAnn'nnTTC
Cuts at: 3674 3674
Size: 5545
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3353 276
Size: 53 83 186 936 1819 2468
Fragments arranged by size:
2468 1819 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PsrI RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI XcmI