Restriction map for
pFN21AE2173
(Circular) (Six-base) MAPSORT of: pFN21AE2173.seq Check: 4593 from: 1 to: 6898
pFN21AE2173 6898 bp
With 182 enzymes: SgfI *
March 28, 2012 13:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4708 278
Size: 53 83 186 936 3172 2468
Fragments arranged by size:
3172 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4241 1937
Size: 2304 4594
Acc65I G'GTAC_C
Cuts at: 4219 4219
Size: 6898
AclI AA'CG_TT
Cuts at: 1563 5025 5398 1563
Size: 3462 373 3063
Fragments arranged by size:
3462 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2898 3038 3107 3638 4961 6345 6613 1632
Size: 1266 140 69 531 1323 1384 268 1917
Fragments arranged by size:
1917 1384 1323 1266 531 268 140 69
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6701
AflIII A'CryG_T
Cuts at: 1203 5797 6774 1203
Size: 4594 977 1327
Fragments arranged by size:
4594 1327 977
AgeI A'CCGG_T
Cuts at: 5729 5729
Size: 6898
AhdI GACnn_n'nnGTC
Cuts at: 5627 5627
Size: 6898
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6898
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3974 1184
Size: 2790 4108
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3254 3286 3254
Size: 32 6866
AlwNI CAG_nnn'CTG
Cuts at: 2204 2357 2704 2947 3457 3553 4641 6213 2204
Size: 153 347 243 510 96 1088 1572 2889
Fragments arranged by size:
2889 1572 1088 510 347 243 153 96
ApaLI G'TGCA_C
Cuts at: 4955 6111 4955
Size: 1156 5742
ApoI r'AATT_y
Cuts at: 1493 2524 3166 3702 4068 4207 4433 4585 6694
Size: 1031 642 536 366 139 226 152 2109
Cuts at: 6694 1493
Size: 1697
Fragments arranged by size:
2109 1697 1031 642 536 366 226 152
139
AseI AT'TA_AT
Cuts at: 160 1032 3287 3921 5452 160
Size: 872 2255 634 1531 1606
Fragments arranged by size:
2255 1606 1531 872 634
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6898
AvaI C'yCGr_G
Cuts at: 1870 1943 2844 4223 1870
Size: 73 901 1379 4545
Fragments arranged by size:
4545 1379 901 73
AvrII C'CTAG_G
Cuts at: 2315 2315
Size: 6898
BamHI G'GATC_C
Cuts at: 4228 4595 4228
Size: 367 6531
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4219 5674 618
Size: 325 217 627 2432 1455 1842
Fragments arranged by size:
2432 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2462 2860 4217 1391
Size: 1071 398 1357 4072
Fragments arranged by size:
4072 1357 1071 398
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3906 928
Size: 971 2007 3920
Fragments arranged by size:
3920 2007 971
BbvCI CC'TCA_GC
Cuts at: 2705 2705
Size: 6898
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3113 3147 5089 5123 1903
Size: 34 1176 34 1942 34 3678
Fragments arranged by size:
3678 1942 1176 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4790 6000 4790
Size: 1210 5688
BclI T'GATC_A
Cuts at: 1548 2059 4045 1548
Size: 511 1986 4401
Fragments arranged by size:
4401 1986 511
BfrBI ATG'CAT
Cuts at: 2306 2306
Size: 6898
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5509 136
Size: 107 122 71 631 605 3837 1525
Fragments arranged by size:
3837 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2041 3646 6893 2041
Size: 1605 3247 2046
Fragments arranged by size:
3247 2046 1605
BlpI GC'TnA_GC
Cuts at: 4315 4315
Size: 6898
Bme1580I G_kGCm'C
Cuts at: 1790 4959 6115 1790
Size: 3169 1156 2573
Fragments arranged by size:
3169 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6898
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2487 4629 5587
Size: 416 53 83 399 24 1070 2142 958
Cuts at: 5587 442
Size: 1753
Fragments arranged by size:
2142 1753 1070 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6898
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2711 2912 3629 3839 3881 5558 1239
Size: 1472 201 717 210 42 1677 2579
Fragments arranged by size:
2579 1677 1472 717 210 201 42
Bpu10I CC'TnA_GC
Cuts at: 2705 2705
Size: 6898
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2754 5023 5888 6186 2754
Size: 2269 865 298 3466
Fragments arranged by size:
3466 2269 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5561 882
Size: 617 4062 2219
Fragments arranged by size:
4062 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2187 4689 493
Size: 711 983 2502 2702
Fragments arranged by size:
2702 2502 983 711
BsaBI GATnn'nnATC
Cuts at: 3651 6892 3651
Size: 3241 3657
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4705 5087 5722 275
Size: 53 83 186 936 3172 382 635 1451
Fragments arranged by size:
3172 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5331 5729 6003 6150 6561 5331
Size: 398 274 147 411 5668
Fragments arranged by size:
5668 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6673
Fragments arranged by size:
6673 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6898
BseYI C'CCAG_C
Cuts at: 2901 3569 6101 6597 2901
Size: 668 2532 496 3202
Fragments arranged by size:
3202 2532 668 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3117 1163
Size: 573 1381 4944
Fragments arranged by size:
4944 1381 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4348 5109 6137 1540
Size: 275 186 2347 761 1028 2301
Fragments arranged by size:
2347 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2462 4959 5044 6115 2462
Size: 2497 85 1071 3245
Fragments arranged by size:
3245 2497 1071 85
BsmI GAATG_Cn'
Cuts at: 2818 4409 4502 2818
Size: 1591 93 5214
Fragments arranged by size:
5214 1591 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3687 3687
Size: 6898
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2462 2860 4217 4959 5044 6115 1391
Size: 399 672 398 1357 742 85 1071 2174
Fragments arranged by size:
2174 1357 1071 742 672 399 398 85
BspEI T'CCGG_A
Cuts at: 6561 6561
Size: 6898
BspHI T'CATG_A
Cuts at: 4140 4787 6517 4140
Size: 647 1730 4521
Fragments arranged by size:
4521 1730 647
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4253 844
Size: 3409 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4785 1394
Size: 429 2962 3507
Fragments arranged by size:
3507 2962 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3668 5393 5567 65
Size: 1183 2420 1725 174 1396
Fragments arranged by size:
2420 1725 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5542 5729 1700
Size: 110 3732 187 2869
Fragments arranged by size:
3732 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6898
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6898
BssSI C'ACGA_G
Cuts at: 4958 5970 4958
Size: 1012 5886
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6898
BstBI TT'CG_AA
Cuts at: 2215 5702 2215
Size: 3487 3411
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6898
BstXI CCAn_nnnn'nTGG
Cuts at: 3400 3569 4190 3400
Size: 169 621 6108
Fragments arranged by size:
6108 621 169
BstYI r'GATC_y
Cuts at: 1972 2041 2264 2756 3482 3550 3646 4228 4595
Size: 69 223 492 726 68 96 582 367
Cuts at: 4595 4980 4997 6449 6535 6547 6893 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 726 582 492 385 367 346
223 96 86 69 68 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6346
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6159
Fragments arranged by size:
6159 683 56
BtsI GCAGTG_nn'
Cuts at: 2244 3387 4414 5208 5228 2244
Size: 1143 1027 794 20 3914
Fragments arranged by size:
3914 1143 1027 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6898
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6863
DraI TTT'AAA
Cuts at: 2049 2727 4202 2049
Size: 678 1475 4745
Fragments arranged by size:
4745 1475 678
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5905 809
Size: 507 4589 1802
Fragments arranged by size:
4589 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3189 4345 5234
Size: 54 1425 50 275 18 1359 1156 889
Cuts at: 5234 6863 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1359 1156 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4345 1537
Size: 275 2533 4090
Fragments arranged by size:
4090 2533 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3057 3083 3212 4828 1353
Size: 1704 26 129 1616 3423
Fragments arranged by size:
3423 1704 1616 129 26
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3877 5485 5859 6005 387
Size: 1064 138 2288 1608 374 146 1280
Fragments arranged by size:
2288 1608 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2460 2460
Size: 6898
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2711 2898 2912 3038 3107 3629 3638
Size: 393 1079 187 14 126 69 522 9
Cuts at: 3638 3839 3881 4961 5558 6345 6613 1239
Size: 201 42 1080 597 787 268 1524
Fragments arranged by size:
1524 1080 1079 787 597 522 393 268
201 187 126 69 42 14 9
EcoRI G'AATT_C
Cuts at: 4207 4585 4207
Size: 378 6520
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3280 3665 3280
Size: 385 6513
FspI TGC'GCA
Cuts at: 5404 5404
Size: 6898
HaeII r_GCGC'y
Cuts at: 1678 3868 6045 1678
Size: 2190 2177 2531
Fragments arranged by size:
2531 2190 2177
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2648 2680 5539 5571 5613
Size: 32 135 32 1076 32 2859 32 42
Cuts at: 5613 5645 1373
Size: 32 2626
Fragments arranged by size:
2859 2626 1076 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4155 4242 4488 669
Size: 988 281 2217 87 246 3079
Fragments arranged by size:
3079 2217 988 281 246 87
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6898
HpaI GTT'AAC
Cuts at: 4488 4488
Size: 6898
KpnI G_GTAC'C
Cuts at: 4223 4223
Size: 6898
MfeI C'AATT_G
Cuts at: 4041 4497 4041
Size: 456 6442
MluI A'CGCG_T
Cuts at: 6774 6774
Size: 6898
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6012 6196 1305
Size: 4707 184 2007
Fragments arranged by size:
4707 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5076
Fragments arranged by size:
5076 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4856 5215 5374 518
Size: 648 3690 359 159 2042
Fragments arranged by size:
3690 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2204 2701 2905 4314 4991 6139 6384 1606
Size: 598 497 204 1409 677 1148 245 2120
Fragments arranged by size:
2120 1409 1148 677 598 497 245 204
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6346
NdeI CA'TA_TG
Cuts at: 387 4189 387
Size: 3802 3096
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6898
NotI GC'GGCC_GC
Cuts at: 4345 4345
Size: 6898
NsiI A_TGCA'T
Cuts at: 2308 2308
Size: 6898
NspI r_CATG'y
Cuts at: 1132 1229 4256 5801 1132
Size: 97 3027 1545 2229
Fragments arranged by size:
3027 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6898
PciI A'CATG_T
Cuts at: 5797 5797
Size: 6898
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 1108
Size: 902 5996
PfoI T'CCnGG_A
Cuts at: 2928 2928
Size: 6898
PmeI GTTT'AAAC
Cuts at: 4202 4202
Size: 6898
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3875 3907 4967 4999 6480 6512 3875
Size: 32 1060 32 1481 32 4261
Fragments arranged by size:
4261 1481 1060 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2447 1613
Size: 834 6064
PsiI TTA'TAA
Cuts at: 4468 4468
Size: 6898
PspXI vC'TCGA_Gb
Cuts at: 2844 2844
Size: 6898
PstI C_TGCA'G
Cuts at: 830 3033 3048 3237 4250 830
Size: 2203 15 189 1013 3478
Fragments arranged by size:
3478 2203 1013 189 15
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6898
PvuII CAG'CTG
Cuts at: 2204 2701 2905 2204
Size: 497 204 6197
Fragments arranged by size:
6197 497 204
SacI G_AGCT'C
Cuts at: 2462 2462
Size: 6898
SalI G'TCGA_C
Cuts at: 1936 4240 1936
Size: 2304 4594
SbfI CC_TGCA'GG
Cuts at: 4250 4250
Size: 6898
ScaI AGT'ACT
Cuts at: 2953 5146 6858 2953
Size: 2193 1712 2993
Fragments arranged by size:
2993 2193 1712
SfcI C'TryA_G
Cuts at: 826 1045 3029 3044 3233 4246 5381 6062 6253
Size: 219 1984 15 189 1013 1135 681 191
Cuts at: 6253 826
Size: 1471
Fragments arranged by size:
1984 1471 1135 1013 681 219 191 189
15
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6898
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6898
SmaI CCC'GGG
Cuts at: 1872 4225 1872
Size: 2353 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2769 2844 5002 5903 6165 820
Size: 197 926 826 75 2158 901 262 1553
Fragments arranged by size:
2158 1553 926 901 826 262 197 75
SnaBI TAC'GTA
Cuts at: 493 2187 4689 493
Size: 1694 2502 2702
Fragments arranged by size:
2702 2502 1694
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6898
SphI G_CATG'C
Cuts at: 1132 4256 1132
Size: 3124 3774
SspI AAT'ATT
Cuts at: 5 52 4822 5
Size: 47 4770 2081
Fragments arranged by size:
4770 2081 47
StuI AGG'CCT
Cuts at: 1517 2285 2320 3375 1517
Size: 768 35 1055 5040
Fragments arranged by size:
5040 1055 768 35
StyI C'CwwG_G
Cuts at: 513 1065 2315 4337 513
Size: 552 1250 2022 3074
Fragments arranged by size:
3074 2022 1250 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4925 4942 5095 5280 5725 325
Size: 1255 3345 17 153 185 445 1498
Fragments arranged by size:
3345 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2911 2951 5144
Size: 274 80 33 51 1443 934 40 2193
Cuts at: 5144 6856 96
Size: 1712 138
Fragments arranged by size:
2193 1712 1443 934 274 138 80 51
40 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6858 6890 6858
Size: 32 6866
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6898
XbaI T'CTAG_A
Cuts at: 4234 4234
Size: 6898
XhoI C'TCGA_G
Cuts at: 1943 2844 1943
Size: 901 5997
XmaI C'CCGG_G
Cuts at: 1870 4223 1870
Size: 2353 4545
XmnI GAAnn'nnTTC
Cuts at: 3414 3702 5027 3414
Size: 288 1325 5285
Fragments arranged by size:
5285 1325 288
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4706 276
Size: 53 83 186 936 3172 2468
Fragments arranged by size:
3172 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BaeI BbeI BplI BsiWI
BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI
PspOMI PsrI RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI