Restriction map for
pFN21AE2154
(Circular) (Six-base) MAPSORT of: pFN21AE2154.seq Check: 8763 from: 1 to: 5746
pFN21AE2154 5746 bp
With 182 enzymes: SgfI *
May 20, 2016 17:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2188 2259 2188
Size: 71 5675
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3556 278
Size: 53 83 186 936 2020 2468
Fragments arranged by size:
2468 2020 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3089 1937
Size: 1152 4594
Acc65I G'GTAC_C
Cuts at: 3067 3067
Size: 5746
AclI AA'CG_TT
Cuts at: 1563 3873 4246 1563
Size: 2310 373 3063
Fragments arranged by size:
3063 2310 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2930 3021 3809 5193 5461 1632
Size: 1298 91 788 1384 268 1917
Fragments arranged by size:
1917 1384 1298 788 268 91
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5549
AflIII A'CryG_T
Cuts at: 1203 4645 5622 1203
Size: 3442 977 1327
Fragments arranged by size:
3442 1327 977
AgeI A'CCGG_T
Cuts at: 4577 4577
Size: 5746
AhdI GACnn_n'nnGTC
Cuts at: 4475 4475
Size: 5746
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5746
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2690 1184
Size: 1506 4240
AlwNI CAG_nnn'CTG
Cuts at: 2779 2923 3489 5061 2779
Size: 144 566 1572 3464
Fragments arranged by size:
3464 1572 566 144
ApaLI G'TGCA_C
Cuts at: 2216 3803 4959 2216
Size: 1587 1156 3003
Fragments arranged by size:
3003 1587 1156
ApoI r'AATT_y
Cuts at: 1493 2231 2333 3055 3281 3433 5542 1493
Size: 738 102 722 226 152 2109 1697
Fragments arranged by size:
2109 1697 738 722 226 152 102
AseI AT'TA_AT
Cuts at: 160 1032 4300 160
Size: 872 3268 1606
Fragments arranged by size:
3268 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5746
AvaI C'yCGr_G
Cuts at: 1870 1943 2401 2546 2831 3071 1870
Size: 73 458 145 285 240 4545
Fragments arranged by size:
4545 458 285 240 145 73
AvrII C'CTAG_G
Cuts at: 2851 2851
Size: 5746
BamHI G'GATC_C
Cuts at: 3076 3443 3076
Size: 367 5379
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2273 2765 3067 4522 618
Size: 325 217 627 486 492 302 1455 1842
Fragments arranged by size:
1842 1455 627 492 486 325 302 217
BanII G_rGCy'C
Cuts at: 1391 2123 2841 3065 1391
Size: 732 718 224 4072
Fragments arranged by size:
4072 732 718 224
BbeI G_GCGC'C
Cuts at: 2277 2277
Size: 5746
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4775
BbvCI CC'TCA_GC
Cuts at: 2098 2357 2419 2098
Size: 259 62 5425
Fragments arranged by size:
5425 259 62
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3937 3971 1903
Size: 34 2000 34 3678
Fragments arranged by size:
3678 2000 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3638 4848 3638
Size: 1210 4536
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5746
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2043 2548 2756
Size: 107 122 71 631 605 371 505 208
Cuts at: 2756 2836 4357 136
Size: 80 1521 1525
Fragments arranged by size:
1525 1521 631 605 505 371 208 122
107 80 71
BglII A'GATC_T
Cuts at: 2692 5741 2692
Size: 3049 2697
BlpI GC'TnA_GC
Cuts at: 3163 3163
Size: 5746
Bme1580I G_kGCm'C
Cuts at: 1790 2220 2832 3807 4963 1790
Size: 430 612 975 1156 2573
Fragments arranged by size:
2573 1156 975 612 430
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5746
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2693 2875 3477
Size: 416 53 83 399 24 1276 182 602
Cuts at: 3477 4435 442
Size: 958 1753
Fragments arranged by size:
1753 1276 958 602 416 399 182 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5746
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2252 2287 2480 2489 4406 1239
Size: 1013 35 193 9 1917 2579
Fragments arranged by size:
2579 1917 1013 193 35 9
Bpu10I CC'TnA_GC
Cuts at: 2098 2117 2357 2419 2924 2098
Size: 19 240 62 505 4920
Fragments arranged by size:
4920 505 240 62 19
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3871 4736 5034 3871
Size: 865 298 4583
Fragments arranged by size:
4583 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2479 2600 4409 882
Size: 617 980 121 1809 2219
Fragments arranged by size:
2219 1809 980 617 121
BsaAI yAC'GTr
Cuts at: 493 1204 3537 493
Size: 711 2333 2702
Fragments arranged by size:
2702 2333 711
BsaBI GATnn'nnATC
Cuts at: 5740 5740
Size: 5746
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2274 3553 3935 4570
Size: 53 83 186 936 741 1279 382 635
Cuts at: 4570 275
Size: 1451
Fragments arranged by size:
1451 1279 936 741 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2532 4179 4577 4851 4998 5409 2532
Size: 1647 398 274 147 411 2869
Fragments arranged by size:
2869 1647 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2462 2492 2585 2607 2615
Size: 30 165 30 1059 30 93 22 8
Cuts at: 2615 2637 1178
Size: 22 4287
Fragments arranged by size:
4287 1059 165 93 30 30 30 22
22 8
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2255 2687 1186
Size: 1069 432 4245
Fragments arranged by size:
4245 1069 432
BseYI C'CCAG_C
Cuts at: 2993 4949 5445 2993
Size: 1956 496 3294
Fragments arranged by size:
3294 1956 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5173
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3196 3957 4985 1540
Size: 275 186 1195 761 1028 2301
Fragments arranged by size:
2301 1195 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2220 3807 3892 4963 2220
Size: 1587 85 1071 3003
Fragments arranged by size:
3003 1587 1071 85
BsmI GAATG_Cn'
Cuts at: 2046 3257 3350 2046
Size: 1211 93 4442
Fragments arranged by size:
4442 1211 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2123 2220 2832 2841 3065 3807 3892
Size: 399 333 97 612 9 224 742 85
Cuts at: 3892 4963 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 612 399 333 224 97
85 9
BspEI T'CCGG_A
Cuts at: 2532 5409 2532
Size: 2877 2869
BspHI T'CATG_A
Cuts at: 3635 5365 3635
Size: 1730 4016
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2188 2208 2259 2724 3101 844
Size: 1344 20 51 465 377 3489
Fragments arranged by size:
3489 1344 465 377 51 20
BsrBI CCG'CTC
Cuts at: 1394 1823 2145 3633 1394
Size: 429 322 1488 3507
Fragments arranged by size:
3507 1488 429 322
BsrDI GCAATG_nn'
Cuts at: 65 1248 2314 4241 4415 65
Size: 1183 1066 1927 174 1396
Fragments arranged by size:
1927 1396 1183 1066 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2762 4390 4577 1700
Size: 110 952 1628 187 2869
Fragments arranged by size:
2869 1628 952 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5746
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5746
BssSI C'ACGA_G
Cuts at: 2476 3806 4818 2476
Size: 1330 1012 3404
Fragments arranged by size:
3404 1330 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2765 794
Size: 1971 3775
BstBI TT'CG_AA
Cuts at: 4550 4550
Size: 5746
BstEII G'GTnAC_C
Cuts at: 1184 2110 1184
Size: 926 4820
BstYI r'GATC_y
Cuts at: 1972 2368 2692 2907 3076 3443 3828 3845 5297
Size: 396 324 215 169 367 385 17 1452
Cuts at: 5297 5383 5395 5741 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 396 385 367 346 324 215
169 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2627 2627
Size: 5746
BtgI C'CryG_G
Cuts at: 513 1065 2152 2226 513
Size: 552 1087 74 4033
Fragments arranged by size:
4033 1087 552 74
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5007
Fragments arranged by size:
5007 683 56
BtsI GCAGTG_nn'
Cuts at: 3036 3262 4056 4076 3036
Size: 226 794 20 4706
Fragments arranged by size:
4706 794 226 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5746
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5711
DraI TTT'AAA
Cuts at: 3050 3050
Size: 5746
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4753 809
Size: 507 3437 1802
Fragments arranged by size:
3437 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2223 2342 2556
Size: 54 1425 50 275 18 393 119 214
Cuts at: 2556 2748 2820 3033 3193 4082 5711 8
Size: 192 72 213 160 889 1629 43
Fragments arranged by size:
1629 1425 889 393 275 214 213 192
160 119 72 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3193 1537
Size: 275 1381 4090
Fragments arranged by size:
4090 1381 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2414 2732 3676 1353
Size: 1061 318 944 3423
Fragments arranged by size:
3423 1061 944 318
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4333 4707 4853 387
Size: 1064 138 2744 374 146 1280
Fragments arranged by size:
2744 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2252 2287 2480 2489 2930 3021 3809
Size: 393 620 35 193 9 441 91 788
Cuts at: 3809 4406 5193 5461 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 788 787 620 597 441 393 268
193 91 35 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2267 2411 2267
Size: 144 5602
EcoO109I rG'GnC_Cy
Cuts at: 2464 2776 2464
Size: 312 5434
EcoRI G'AATT_C
Cuts at: 2231 2333 3055 3433 2231
Size: 102 722 378 4544
Fragments arranged by size:
4544 722 378 102
FspI TGC'GCA
Cuts at: 2576 4252 2576
Size: 1676 4070
HaeII r_GCGC'y
Cuts at: 1678 2277 4893 1678
Size: 599 2616 2531
Fragments arranged by size:
2616 2531 599
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4387 4419 4461 4493 1373
Size: 32 135 32 2815 32 42 32 2626
Fragments arranged by size:
2815 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2174 3090 3336 669
Size: 988 281 236 916 246 3079
Fragments arranged by size:
3079 988 916 281 246 236
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5746
HpaI GTT'AAC
Cuts at: 3336 3336
Size: 5746
KasI G'GCGC_C
Cuts at: 2273 2273
Size: 5746
KpnI G_GTAC'C
Cuts at: 3071 3071
Size: 5746
MfeI C'AATT_G
Cuts at: 3345 3345
Size: 5746
MluI A'CGCG_T
Cuts at: 5622 5622
Size: 5746
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2583 4860 5044 1305
Size: 1278 2277 184 2007
Fragments arranged by size:
2277 2007 1278 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2558 2750 2822 3035 10
Size: 54 1425 343 726 192 72 213 2721
Fragments arranged by size:
2721 1425 726 343 213 192 72 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3704 4063 4222 518
Size: 648 2538 359 159 2042
Fragments arranged by size:
2538 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2356 2458 2638 2788 3162 3839 4987 5232
Size: 750 102 180 150 374 677 1148 245
Cuts at: 5232 1606
Size: 2120
Fragments arranged by size:
2120 1148 750 677 374 245 180 150
102
NarI GG'CG_CC
Cuts at: 2274 2274
Size: 5746
NcoI C'CATG_G
Cuts at: 513 1065 2152 513
Size: 552 1087 4107
Fragments arranged by size:
4107 1087 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5746
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5746
NotI GC'GGCC_GC
Cuts at: 3193 3193
Size: 5746
NspI r_CATG'y
Cuts at: 1132 1229 2797 2875 3104 4649 1132
Size: 97 1568 78 229 1545 2229
Fragments arranged by size:
2229 1568 1545 229 97 78
PasI CC'CwG_GG
Cuts at: 1348 2039 1348
Size: 691 5055
PciI A'CATG_T
Cuts at: 4645 4645
Size: 5746
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5746
PfoI T'CCnGG_A
Cuts at: 2466 2466
Size: 5746
PmeI GTTT'AAAC
Cuts at: 3050 3050
Size: 5746
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3815 3847 5328 5360 3815
Size: 32 1481 32 4201
Fragments arranged by size:
4201 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2464 2464
Size: 5746
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5746
PsiI TTA'TAA
Cuts at: 3316 3316
Size: 5746
PstI C_TGCA'G
Cuts at: 830 3098 830
Size: 2268 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5746
PvuII CAG'CTG
Cuts at: 2356 2458 2788 2356
Size: 102 330 5314
Fragments arranged by size:
5314 330 102
SalI G'TCGA_C
Cuts at: 1936 3088 1936
Size: 1152 4594
SbfI CC_TGCA'GG
Cuts at: 3098 3098
Size: 5746
ScaI AGT'ACT
Cuts at: 2682 3994 5706 2682
Size: 1312 1712 2722
Fragments arranged by size:
2722 1712 1312
SfcI C'TryA_G
Cuts at: 826 1045 3094 4229 4910 5101 826
Size: 219 2049 1135 681 191 1471
Fragments arranged by size:
2049 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2275 2275
Size: 5746
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5746
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5746
SmaI CCC'GGG
Cuts at: 1872 2548 3073 1872
Size: 676 525 4545
Fragments arranged by size:
4545 676 525
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3850 4751 5013 820
Size: 197 926 1907 901 262 1553
Fragments arranged by size:
1907 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3537 493
Size: 3044 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5746
SphI G_CATG'C
Cuts at: 1132 3104 1132
Size: 1972 3774
SspI AAT'ATT
Cuts at: 5 52 3670 5
Size: 47 3618 2081
Fragments arranged by size:
3618 2081 47
StuI AGG'CCT
Cuts at: 1517 2417 1517
Size: 900 4846
StyI C'CwwG_G
Cuts at: 513 1065 2152 2587 2851 3185 513
Size: 552 1087 435 264 334 3074
Fragments arranged by size:
3074 1087 552 435 334 264
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3773 3790 3943 4128 4573 325
Size: 1255 2193 17 153 185 445 1498
Fragments arranged by size:
2193 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2680 3992 5704
Size: 274 80 33 51 1443 703 1312 1712
Cuts at: 5704 96
Size: 138
Fragments arranged by size:
1712 1443 1312 703 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2462 2494 2607 2639 5706 5738 2462
Size: 32 113 32 3067 32 2470
Fragments arranged by size:
3067 2470 113 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5746
XbaI T'CTAG_A
Cuts at: 2063 2659 3082 2063
Size: 596 423 4727
Fragments arranged by size:
4727 596 423
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2311 2815 2311
Size: 504 5242
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5746
XmaI C'CCGG_G
Cuts at: 1870 2546 3071 1870
Size: 676 525 4545
Fragments arranged by size:
4545 676 525
XmnI GAAnn'nnTTC
Cuts at: 3875 3875
Size: 5746
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3554 276
Size: 53 83 186 936 2020 2468
Fragments arranged by size:
2468 2020 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SrfI SwaI