Restriction map for
pFN21AE2149
(Circular) (Six-base) MAPSORT of: pFN21AE2149.seq Check: 8614 from: 1 to: 6526
pFN21AE2149 6526 bp
With 182 enzymes: SgfI *
March 28, 2012 12:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2064 3160 3427 2064
Size: 1096 267 5163
Fragments arranged by size:
5163 1096 267
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4336 278
Size: 53 83 186 936 2800 2468
Fragments arranged by size:
2800 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2415 3869 1937
Size: 478 1454 4594
Fragments arranged by size:
4594 1454 478
Acc65I G'GTAC_C
Cuts at: 2253 3847 2253
Size: 1594 4932
AclI AA'CG_TT
Cuts at: 1563 4653 5026 1563
Size: 3090 373 3063
Fragments arranged by size:
3090 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2342 2555 3219 4589 5973 6241 1632
Size: 710 213 664 1370 1384 268 1917
Fragments arranged by size:
1917 1384 1370 710 664 268 213
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6329
AflIII A'CryG_T
Cuts at: 1203 2295 2787 5425 6402 1203
Size: 1092 492 2638 977 1327
Fragments arranged by size:
2638 1327 1092 977 492
AgeI A'CCGG_T
Cuts at: 5357 5357
Size: 6526
AhdI GACnn_n'nnGTC
Cuts at: 5255 5255
Size: 6526
AleI CACnn'nnGTG
Cuts at: 1166 2298 2843 1166
Size: 1132 545 4849
Fragments arranged by size:
4849 1132 545
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6526
AlwNI CAG_nnn'CTG
Cuts at: 2149 2747 3044 3242 3701 4269 5841 2149
Size: 598 297 198 459 568 1572 2834
Fragments arranged by size:
2834 1572 598 568 459 297 198
ApaI G_GGCC'C
Cuts at: 2080 2088 3405 3679 2080
Size: 8 1317 274 4927
Fragments arranged by size:
4927 1317 274 8
ApaLI G'TGCA_C
Cuts at: 3094 4583 5739 3094
Size: 1489 1156 3881
Fragments arranged by size:
3881 1489 1156
ApoI r'AATT_y
Cuts at: 1493 3835 4061 4213 6322 1493
Size: 2342 226 152 2109 1697
Fragments arranged by size:
2342 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5080 160
Size: 872 4048 1606
Fragments arranged by size:
4048 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6526
AvaI C'yCGr_G
Cuts at: 1870 1943 2080 2177 2283 3481 3507 3851 1870
Size: 73 137 97 106 1198 26 344 4545
Fragments arranged by size:
4545 1198 344 137 106 97 73 26
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2245 2278 2245
Size: 33 6493
BamHI G'GATC_C
Cuts at: 3856 4223 3856
Size: 367 6159
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2052 2123 2253 2837 2855
Size: 325 217 627 265 71 130 584 18
Cuts at: 2855 2880 3062 3511 3847 5302 618
Size: 25 182 449 336 1455 1842
Fragments arranged by size:
1842 1455 627 584 449 336 325 265
217 182 130 71 25 18
BanII G_rGCy'C
Cuts at: 1391 2080 2088 2379 2675 2918 3186 3405 3679
Size: 689 8 291 296 243 268 219 274
Cuts at: 3679 3845 1391
Size: 166 4072
Fragments arranged by size:
4072 689 296 291 274 268 243 219
166 8
BbeI G_GCGC'C
Cuts at: 2884 2884
Size: 6526
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5555
BbvCI CC'TCA_GC
Cuts at: 3582 3582
Size: 6526
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2641 2675 2888 2922 4717 4751 1903
Size: 34 704 34 213 34 1795 34 3678
Fragments arranged by size:
3678 1795 704 213 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4418 5628 4418
Size: 1210 5316
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6526
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2050 3347 3428
Size: 107 122 71 631 605 378 1297 81
Cuts at: 3428 3740 5137 136
Size: 312 1397 1525
Fragments arranged by size:
1525 1397 1297 631 605 378 312 122
107 81 71
BglII A'GATC_T
Cuts at: 6521 6521
Size: 6526
BlpI GC'TnA_GC
Cuts at: 2555 2706 2972 3243 3562 3943 2555
Size: 151 266 271 319 381 5138
Fragments arranged by size:
5138 381 319 271 266 151
Bme1580I G_kGCm'C
Cuts at: 1790 2080 2088 2126 2178 2234 2787 3038 3098
Size: 290 8 38 52 56 553 251 60
Cuts at: 3098 3195 3222 3360 3405 3474 3516 3643 3679
Size: 97 27 138 45 69 42 127 36
Cuts at: 3679 4587 5743 1790
Size: 908 1156 2573
Fragments arranged by size:
2573 1156 908 553 290 251 138 127
97 69 60 56 52 45 42 38
36 27 8
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6526
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3347 3630 3730
Size: 416 53 83 399 24 1930 283 100
Cuts at: 3730 4257 5215 442
Size: 527 958 1753
Fragments arranged by size:
1930 1753 958 527 416 399 283 100
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6526
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3793 3793
Size: 6526
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2612 2915 3147 3275 3560 3696 5186 1239
Size: 1373 303 232 128 285 136 1490 2579
Fragments arranged by size:
2579 1490 1373 303 285 232 136 128
Bpu10I CC'TnA_GC
Cuts at: 2046 3582 3690 2046
Size: 1536 108 4882
Fragments arranged by size:
4882 1536 108
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4651 5516 5814 4651
Size: 865 298 5363
Fragments arranged by size:
5363 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2440 5189 882
Size: 617 941 2749 2219
Fragments arranged by size:
2749 2219 941 617
BsaAI yAC'GTr
Cuts at: 493 1204 2173 2235 2266 2296 2788 4317 493
Size: 711 969 62 31 30 492 1529 2702
Fragments arranged by size:
2702 1529 969 711 492 62 31 30
BsaBI GATnn'nnATC
Cuts at: 6520 6520
Size: 6526
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2183 2881 4333 4715
Size: 53 83 186 936 650 698 1452 382
Cuts at: 4715 5350 275
Size: 635 1451
Fragments arranged by size:
1452 1451 936 698 650 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4959 5357 5631 5778 6189 4959
Size: 398 274 147 411 5296
Fragments arranged by size:
5296 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2746 2776 3248 3278 3352
Size: 30 165 30 1343 30 472 30 74
Cuts at: 3352 3382 1178
Size: 30 4322
Fragments arranged by size:
4322 1343 472 165 74 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2291 3272 1186
Size: 1105 981 4440
Fragments arranged by size:
4440 1105 981
BseYI C'CCAG_C
Cuts at: 2062 2775 2932 3282 3570 3608 3706 5729 6225
Size: 713 157 350 288 38 98 2023 496
Cuts at: 6225 2062
Size: 2363
Fragments arranged by size:
2363 2023 713 496 350 288 157 98
38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2257 3399 1163
Size: 573 521 1142 4290
Fragments arranged by size:
4290 1142 573 521
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2634 2868 3976 4737 5765 1540
Size: 275 186 633 234 1108 761 1028 2301
Fragments arranged by size:
2301 1108 1028 761 633 275 234 186
BsiHKAI G_wGCw'C
Cuts at: 2449 3098 4587 4672 5743 2449
Size: 649 1489 85 1071 3232
Fragments arranged by size:
3232 1489 1071 649 85
BsmI GAATG_Cn'
Cuts at: 2295 4037 4130 2295
Size: 1742 93 4691
Fragments arranged by size:
4691 1742 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2080 2088 2126 2178 2234 2379 2449
Size: 399 290 8 38 52 56 145 70
Cuts at: 2449 2675 2787 2918 3038 3098 3186 3195 3222
Size: 226 112 131 120 60 88 9 27
Cuts at: 3222 3360 3405 3474 3516 3643 3679 3845 4587
Size: 138 45 69 42 127 36 166 742
Cuts at: 4587 4672 5743 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 290 226 166 145
138 131 127 120 112 88 85 70
69 60 56 52 45 42 38 36
27 9 8
BspEI T'CCGG_A
Cuts at: 6189 6189
Size: 6526
BspHI T'CATG_A
Cuts at: 4415 6145 4415
Size: 1730 4796
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2064 2105 3160 3427 3807 3881 844
Size: 1220 41 1055 267 380 74 3489
Fragments arranged by size:
3489 1220 1055 380 267 74 41
BsrBI CCG'CTC
Cuts at: 1394 1823 2050 4413 1394
Size: 429 227 2363 3507
Fragments arranged by size:
3507 2363 429 227
BsrDI GCAATG_nn'
Cuts at: 65 1248 5021 5195 65
Size: 1183 3773 174 1396
Fragments arranged by size:
3773 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3065 5170 5357 1700
Size: 110 1255 2105 187 2869
Fragments arranged by size:
2869 2105 1255 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6526
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6526
BssSI C'ACGA_G
Cuts at: 4586 5598 4586
Size: 1012 5514
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2149 2747 794
Size: 1355 598 4573
Fragments arranged by size:
4573 1355 598
BstBI TT'CG_AA
Cuts at: 5330 5330
Size: 6526
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6526
BstYI r'GATC_y
Cuts at: 1972 2404 2511 3856 4223 4608 4625 6077 6163
Size: 432 107 1345 367 385 17 1452 86
Cuts at: 6163 6175 6521 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1345 432 385 367 346 107
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2923 3396 3516 513
Size: 552 1858 473 120 3523
Fragments arranged by size:
3523 1858 552 473 120
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2342 3262 487
Size: 683 56 1116 920 3751
Fragments arranged by size:
3751 1116 920 683 56
BtsI GCAGTG_nn'
Cuts at: 2859 4042 4836 4856 2859
Size: 1183 794 20 4529
Fragments arranged by size:
4529 1183 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6526
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3580 3615 529
Size: 35 3016 35 3440
Fragments arranged by size:
3440 3016 35 35
DraI TTT'AAA
Cuts at: 3830 3830
Size: 6526
DraIII CAC_nnn'GTG
Cuts at: 2566 2566
Size: 6526
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5533 809
Size: 507 4217 1802
Fragments arranged by size:
4217 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2022 2631 2865
Size: 54 1425 50 275 18 192 609 234
Cuts at: 2865 3273 3429 3973 4862 6491 8
Size: 408 156 544 889 1629 43
Fragments arranged by size:
1629 1425 889 609 544 408 275 234
192 156 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2631 2865 3973 1537
Size: 275 819 234 1108 4090
Fragments arranged by size:
4090 1108 819 275 234
EarI CTCTTCn'nnn_
Cuts at: 1353 4456 1353
Size: 3103 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2046 2170 2669 5113 5487 5633
Size: 1064 138 457 124 499 2444 374 146
Cuts at: 5633 387
Size: 1280
Fragments arranged by size:
2444 1280 1064 499 457 374 146 138
124
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2342 2555 2612 2915 3147 3219 3275
Size: 393 710 213 57 303 232 72 56
Cuts at: 3275 3560 3696 4589 5186 5973 6241 1239
Size: 285 136 893 597 787 268 1524
Fragments arranged by size:
1524 893 787 710 597 393 303 285
268 232 213 136 72 57 56
EcoNI CCTnn'n_nnAGG
Cuts at: 2465 2465
Size: 6526
EcoO109I rG'GnC_Cy
Cuts at: 2076 2077 2084 2085 2716 3401 3402 3612 3675
Size: 1 7 1 631 685 1 210 63
Cuts at: 3675 3676 2076
Size: 1 4926
Fragments arranged by size:
4926 685 631 210 63 7 1 1
1 1
EcoRI G'AATT_C
Cuts at: 3835 4213 3835
Size: 378 6148
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2455 2455
Size: 6526
FspI TGC'GCA
Cuts at: 5032 5032
Size: 6526
HaeII r_GCGC'y
Cuts at: 1678 2884 3013 3134 3701 5673 1678
Size: 1206 129 121 567 1972 2531
Fragments arranged by size:
2531 1972 1206 567 129 121
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2981 3013 3040 3072 3248
Size: 32 135 32 1409 32 27 32 176
Cuts at: 3248 3280 5167 5199 5241 5273 1373
Size: 32 1887 32 42 32 2626
Fragments arranged by size:
2626 1887 1409 176 135 42 32 32
32 32 32 32 32 27
HincII GTy'rAC
Cuts at: 669 1657 1938 2416 3870 4116 669
Size: 988 281 478 1454 246 3079
Fragments arranged by size:
3079 1454 988 478 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6526
HpaI GTT'AAC
Cuts at: 4116 4116
Size: 6526
KasI G'GCGC_C
Cuts at: 2880 2880
Size: 6526
KpnI G_GTAC'C
Cuts at: 2257 3851 2257
Size: 1594 4932
MfeI C'AATT_G
Cuts at: 4125 4125
Size: 6526
MluI A'CGCG_T
Cuts at: 6402 6402
Size: 6526
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3699 3804 5640 5824 1305
Size: 2394 105 1836 184 2007
Fragments arranged by size:
2394 2007 1836 184 105
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3275 10
Size: 54 1425 343 1443 3261
Fragments arranged by size:
3261 1443 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2298 2843 4484 4843 5002 518
Size: 648 1132 545 1641 359 159 2042
Fragments arranged by size:
2042 1641 1132 648 545 359 159
MspA1I CmG'CkG
Cuts at: 1606 2028 2508 2747 2902 2971 3203 3942 4619
Size: 422 480 239 155 69 232 739 677
Cuts at: 4619 5767 6012 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 739 677 480 422 245 239
232 155 69
NaeI GCC'GGC
Cuts at: 3067 3067
Size: 6526
NarI GG'CG_CC
Cuts at: 2881 2881
Size: 6526
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5974
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6526
NgoMIV G'CCGG_C
Cuts at: 3065 3065
Size: 6526
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6526
NotI GC'GGCC_GC
Cuts at: 2865 3973 2865
Size: 1108 5418
NspI r_CATG'y
Cuts at: 1132 1229 3457 3884 5429 1132
Size: 97 2228 427 1545 2229
Fragments arranged by size:
2229 2228 1545 427 97
PasI CC'CwG_GG
Cuts at: 1348 3644 1348
Size: 2296 4230
PciI A'CATG_T
Cuts at: 5425 5425
Size: 6526
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2566 1108
Size: 1458 5068
PmeI GTTT'AAAC
Cuts at: 3830 3830
Size: 6526
PmlI CAC'GTG
Cuts at: 2235 2266 2296 2788 2235
Size: 31 30 492 5973
Fragments arranged by size:
5973 492 31 30
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4595 4627 6108 6140 4595
Size: 32 1481 32 4981
Fragments arranged by size:
4981 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6526
PsiI TTA'TAA
Cuts at: 4096 4096
Size: 6526
PspOMI G'GGCC_C
Cuts at: 2076 2084 3401 3675 2076
Size: 8 1317 274 4927
Fragments arranged by size:
4927 1317 274 8
PstI C_TGCA'G
Cuts at: 830 2102 2854 3009 3202 3381 3878 830
Size: 1272 752 155 193 179 497 3478
Fragments arranged by size:
3478 1272 752 497 193 179 155
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6526
PvuII CAG'CTG
Cuts at: 2747 2902 2971 3203 2747
Size: 155 69 232 6070
Fragments arranged by size:
6070 232 155 69
SalI G'TCGA_C
Cuts at: 1936 2414 3868 1936
Size: 478 1454 4594
Fragments arranged by size:
4594 1454 478
SbfI CC_TGCA'GG
Cuts at: 2102 3878 2102
Size: 1776 4750
ScaI AGT'ACT
Cuts at: 4774 6486 4774
Size: 1712 4814
SfcI C'TryA_G
Cuts at: 826 1045 2098 2850 3005 3198 3377 3765 3874
Size: 219 1053 752 155 193 179 388 109
Cuts at: 3874 5009 5690 5881 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1053 752 681 388 219 193
191 179 155 109
SfoI GGC'GCC
Cuts at: 2882 2882
Size: 6526
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6526
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6526
SmaI CCC'GGG
Cuts at: 1872 2082 2285 3483 3509 3853 1872
Size: 210 203 1198 26 344 4545
Fragments arranged by size:
4545 1198 344 210 203 26
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4630 5531 5793 820
Size: 197 926 2687 901 262 1553
Fragments arranged by size:
2687 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4317 493
Size: 3824 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6526
SphI G_CATG'C
Cuts at: 1132 3884 1132
Size: 2752 3774
SspI AAT'ATT
Cuts at: 5 52 4450 5
Size: 47 4398 2081
Fragments arranged by size:
4398 2081 47
StuI AGG'CCT
Cuts at: 1517 2044 1517
Size: 527 5999
StyI C'CwwG_G
Cuts at: 513 1065 2400 3276 3965 513
Size: 552 1335 876 689 3074
Fragments arranged by size:
3074 1335 876 689 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2350 4553 4570 4723 4908 5353 325
Size: 1255 770 2203 17 153 185 445 1498
Fragments arranged by size:
2203 1498 1255 770 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4772 6484 96
Size: 274 80 33 51 1443 2795 1712 138
Fragments arranged by size:
2795 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2280 2312 2746 2778 3352 3384 6486 6518 2280
Size: 32 434 32 574 32 3102 32 2288
Fragments arranged by size:
3102 2288 574 434 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6526
XbaI T'CTAG_A
Cuts at: 3862 3862
Size: 6526
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2407 2407
Size: 6526
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6526
XmaI C'CCGG_G
Cuts at: 1870 2080 2283 3481 3507 3851 1870
Size: 210 203 1198 26 344 4545
Fragments arranged by size:
4545 1198 344 210 203 26
XmnI GAAnn'nnTTC
Cuts at: 4655 4655
Size: 6526
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4334 276
Size: 53 83 186 936 2800 2468
Fragments arranged by size:
2800 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BfrBI BsiWI BsmBI BstXI
BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI NruI NsiI
PacI PfoI PpuMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SrfI SwaI