Restriction map for
pFN21AE2137
(Circular) (Six-base) MAPSORT of: pFN21AE2137.seq Check: 5959 from: 1 to: 7387
pFN21AE2137 7387 bp
With 182 enzymes: SgfI *
January 30, 2014 17:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5197 278
Size: 53 83 186 936 3661 2468
Fragments arranged by size:
3661 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4730 1937
Size: 2793 4594
Acc65I G'GTAC_C
Cuts at: 4708 4708
Size: 7387
AclI AA'CG_TT
Cuts at: 1563 5514 5887 1563
Size: 3951 373 3063
Fragments arranged by size:
3951 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 5450 6834 7102 1632
Size: 3818 1384 268 1917
Fragments arranged by size:
3818 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2326 820
Size: 197 1309 5881
Fragments arranged by size:
5881 1309 197
AflIII A'CryG_T
Cuts at: 1203 3220 6286 7263 1203
Size: 2017 3066 977 1327
Fragments arranged by size:
3066 2017 1327 977
AgeI A'CCGG_T
Cuts at: 6218 6218
Size: 7387
AhdI GACnn_n'nnGTC
Cuts at: 6116 6116
Size: 7387
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7387
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7387
AlwNI CAG_nnn'CTG
Cuts at: 2992 3097 5130 6702 2992
Size: 105 2033 1572 3677
Fragments arranged by size:
3677 2033 1572 105
ApaI G_GGCC'C
Cuts at: 2667 2667
Size: 7387
ApaLI G'TGCA_C
Cuts at: 5444 6600 5444
Size: 1156 6231
ApoI r'AATT_y
Cuts at: 1493 2416 3206 4696 4922 5074 7183 1493
Size: 923 790 1490 226 152 2109 1697
Fragments arranged by size:
2109 1697 1490 923 790 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5941 160
Size: 872 4909 1606
Fragments arranged by size:
4909 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7387
AvaI C'yCGr_G
Cuts at: 1870 1943 2970 4712 1870
Size: 73 1027 1742 4545
Fragments arranged by size:
4545 1742 1027 73
AvrII C'CTAG_G
Cuts at: 2294 2294
Size: 7387
BamHI G'GATC_C
Cuts at: 4717 5084 4717
Size: 367 7020
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2703 4708 6163 618
Size: 325 217 627 916 2005 1455 1842
Fragments arranged by size:
2005 1842 1455 916 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2012 2036 2667 3135 4706 1391
Size: 621 24 631 468 1571 4072
Fragments arranged by size:
4072 1571 631 621 468 24
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2049 2856 928
Size: 971 150 807 5459
Fragments arranged by size:
5459 971 807 150
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5578 5612 1903
Size: 34 3641 34 3678
Fragments arranged by size:
3678 3641 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2867 3714 5279 6489 2867
Size: 847 1565 1210 3765
Fragments arranged by size:
3765 1565 1210 847
BclI T'GATC_A
Cuts at: 1548 2886 1548
Size: 1338 6049
BfrBI ATG'CAT
Cuts at: 2573 2573
Size: 7387
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2737 5998 136
Size: 107 122 71 631 605 1065 3261 1525
Fragments arranged by size:
3261 1525 1065 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3018 7382 3018
Size: 4364 3023
BlpI GC'TnA_GC
Cuts at: 4804 4804
Size: 7387
Bme1580I G_kGCm'C
Cuts at: 1790 2667 2706 2971 5448 6604 1790
Size: 877 39 265 2477 1156 2573
Fragments arranged by size:
2573 2477 1156 877 265 39
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7387
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2149 3107 5118
Size: 416 53 83 399 24 732 958 2011
Cuts at: 5118 6076 442
Size: 958 1753
Fragments arranged by size:
2011 1753 958 958 732 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7387
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2237 2490 6047 1239
Size: 998 253 3557 2579
Fragments arranged by size:
3557 2579 998 253
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2482 3544 5512 6377 6675 2482
Size: 1062 1968 865 298 3194
Fragments arranged by size:
3194 1968 1062 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2204 6050 882
Size: 617 705 3846 2219
Fragments arranged by size:
3846 2219 705 617
BsaAI yAC'GTr
Cuts at: 493 1204 5178 493
Size: 711 3974 2702
Fragments arranged by size:
3974 2702 711
BsaBI GATnn'nnATC
Cuts at: 3309 7381 3309
Size: 4072 3315
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2369 5194 5576 6211
Size: 53 83 186 936 836 2825 382 635
Cuts at: 6211 275
Size: 1451
Fragments arranged by size:
2825 1451 936 836 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5820 6218 6492 6639 7050 5820
Size: 398 274 147 411 6157
Fragments arranged by size:
6157 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7162
Fragments arranged by size:
7162 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2042 3229 3266 3695 3779 3863 3901 3947
Size: 856 1187 37 429 84 84 38 46
Cuts at: 3947 3985 4031 4115 4199 4283 4367 4451 4535
Size: 38 46 84 84 84 84 84 84
Cuts at: 4535 1186
Size: 4038
Fragments arranged by size:
4038 1187 856 429 84 84 84 84
84 84 84 84 46 46 38 38
37
BseYI C'CCAG_C
Cuts at: 2771 2809 6590 7086 2771
Size: 38 3781 496 3072
Fragments arranged by size:
3781 3072 496 38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2709 2801 1163
Size: 573 973 92 5749
Fragments arranged by size:
5749 973 573 92
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4837 5598 6626 1540
Size: 275 186 2836 761 1028 2301
Fragments arranged by size:
2836 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3135 5448 5533 6604 3135
Size: 2313 85 1071 3918
Fragments arranged by size:
3918 2313 1071 85
BsmI GAATG_Cn'
Cuts at: 2364 2431 4898 4991 2364
Size: 67 2467 93 4760
Fragments arranged by size:
4760 2467 93 67
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2036 2667 2706 2971 3135 4706
Size: 399 222 24 631 39 265 164 1571
Cuts at: 4706 5448 5533 6604 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1571 1071 742 631 399 265 222
164 85 39 24
BspEI T'CCGG_A
Cuts at: 7050 7050
Size: 7387
BspHI T'CATG_A
Cuts at: 5276 7006 5276
Size: 1730 5657
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3041 4742 844
Size: 2197 1701 3489
Fragments arranged by size:
3489 2197 1701
BsrBI CCG'CTC
Cuts at: 1394 1823 2062 5274 1394
Size: 429 239 3212 3507
Fragments arranged by size:
3507 3212 429 239
BsrDI GCAATG_nn'
Cuts at: 65 1248 5882 6056 65
Size: 1183 4634 174 1396
Fragments arranged by size:
4634 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3858 3942 6031 6218 1700
Size: 110 2048 84 2089 187 2869
Fragments arranged by size:
2869 2089 2048 187 110 84
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7387
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7387
BssSI C'ACGA_G
Cuts at: 5447 6459 5447
Size: 1012 6375
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7387
BstBI TT'CG_AA
Cuts at: 6191 6191
Size: 7387
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7387
BstYI r'GATC_y
Cuts at: 1972 3018 3162 3331 4717 5084 5469 5486 6938
Size: 1046 144 169 1386 367 385 17 1452
Cuts at: 6938 7024 7036 7382 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1386 1046 385 367 346 169
144 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2766 2766
Size: 7387
BtgI C'CryG_G
Cuts at: 513 1065 2070 2659 513
Size: 552 1005 589 5241
Fragments arranged by size:
5241 1005 589 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6648
Fragments arranged by size:
6648 683 56
BtsI GCAGTG_nn'
Cuts at: 2697 4903 5697 5717 2697
Size: 2206 794 20 4367
Fragments arranged by size:
4367 2206 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7387
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7352
DraI TTT'AAA
Cuts at: 4691 4691
Size: 7387
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6394 809
Size: 507 5078 1802
Fragments arranged by size:
5078 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3088 4834 5723
Size: 54 1425 50 275 18 1258 1746 889
Cuts at: 5723 7352 8
Size: 1629 43
Fragments arranged by size:
1746 1629 1425 1258 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4834 1537
Size: 275 3022 4090
Fragments arranged by size:
4090 3022 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2850 2976 3486 5317 1353
Size: 1497 126 510 1831 3423
Fragments arranged by size:
3423 1831 1497 510 126
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5974 6348 6494 387
Size: 1064 138 4385 374 146 1280
Fragments arranged by size:
4385 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3133 3133
Size: 7387
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2237 2490 5450 6047 6834 7102 1239
Size: 393 605 253 2960 597 787 268 1524
Fragments arranged by size:
2960 1524 787 605 597 393 268 253
EcoO109I rG'GnC_Cy
Cuts at: 2664 3426 2664
Size: 762 6625
EcoRI G'AATT_C
Cuts at: 4696 5074 4696
Size: 378 7009
FspI TGC'GCA
Cuts at: 5893 5893
Size: 7387
HaeII r_GCGC'y
Cuts at: 1678 6534 1678
Size: 4856 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 6028 6060 6102 6134 1373
Size: 32 135 32 4456 32 42 32 2626
Fragments arranged by size:
4456 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3218 4731 4977 669
Size: 988 281 1280 1513 246 3079
Fragments arranged by size:
3079 1513 1280 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7387
HpaI GTT'AAC
Cuts at: 4977 4977
Size: 7387
KpnI G_GTAC'C
Cuts at: 4712 4712
Size: 7387
MfeI C'AATT_G
Cuts at: 4986 4986
Size: 7387
MluI A'CGCG_T
Cuts at: 7263 7263
Size: 7387
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3304 6501 6685 1305
Size: 1999 3197 184 2007
Fragments arranged by size:
3197 2007 1999 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5565
Fragments arranged by size:
5565 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5345 5704 5863 518
Size: 648 4179 359 159 2042
Fragments arranged by size:
4179 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2649 2809 4803 5480 6628 6873 1606
Size: 1043 160 1994 677 1148 245 2120
Fragments arranged by size:
2120 1994 1148 1043 677 245 160
NaeI GCC'GGC
Cuts at: 3860 3944 3860
Size: 84 7303
NcoI C'CATG_G
Cuts at: 513 1065 2070 2659 513
Size: 552 1005 589 5241
Fragments arranged by size:
5241 1005 589 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7387
NgoMIV G'CCGG_C
Cuts at: 3858 3942 3858
Size: 84 7303
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7387
NotI GC'GGCC_GC
Cuts at: 4834 4834
Size: 7387
NruI TCG'CGA
Cuts at: 4278 4278
Size: 7387
NsiI A_TGCA'T
Cuts at: 2575 2575
Size: 7387
NspI r_CATG'y
Cuts at: 1132 1229 3224 4745 6290 1132
Size: 97 1995 1521 1545 2229
Fragments arranged by size:
2229 1995 1545 1521 97
PasI CC'CwG_GG
Cuts at: 1348 2735 3299 1348
Size: 1387 564 5436
Fragments arranged by size:
5436 1387 564
PciI A'CATG_T
Cuts at: 3220 6286 3220
Size: 3066 4321
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3097 1108
Size: 1989 5398
PmeI GTTT'AAAC
Cuts at: 4691 4691
Size: 7387
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5456 5488 6969 7001 5456
Size: 32 1481 32 5842
Fragments arranged by size:
5842 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7387
PsiI TTA'TAA
Cuts at: 4957 4957
Size: 7387
PspOMI G'GGCC_C
Cuts at: 2663 2663
Size: 7387
PstI C_TGCA'G
Cuts at: 830 3668 3752 3836 3920 4004 4088 4172 4256
Size: 2838 84 84 84 84 84 84 84
Cuts at: 4256 4340 4424 4508 4592 4676 4739 830
Size: 84 84 84 84 84 63 3478
Fragments arranged by size:
3478 2838 84 84 84 84 84 84
84 84 84 84 84 84 63
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7387
PvuII CAG'CTG
Cuts at: 2649 2649
Size: 7387
SacI G_AGCT'C
Cuts at: 3135 3135
Size: 7387
SalI G'TCGA_C
Cuts at: 1936 4729 1936
Size: 2793 4594
SapI GCTCTTCn'nnn_
Cuts at: 2976 2976
Size: 7387
SbfI CC_TGCA'GG
Cuts at: 4739 4739
Size: 7387
ScaI AGT'ACT
Cuts at: 5635 7347 5635
Size: 1712 5675
SexAI A'CCwGG_T
Cuts at: 2991 2991
Size: 7387
SfcI C'TryA_G
Cuts at: 826 1045 2155 2193 3025 3463 3664 3748 3832
Size: 219 1110 38 832 438 201 84 84
Cuts at: 3832 3916 4000 4084 4168 4252 4336 4420 4504
Size: 84 84 84 84 84 84 84 84
Cuts at: 4504 4588 4672 4735 5870 6551 6742 826
Size: 84 84 63 1135 681 191 1471
Fragments arranged by size:
1471 1135 1110 832 681 438 219 201
191 84 84 84 84 84 84 84
84 84 84 84 84 63 38
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7387
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7387
SmaI CCC'GGG
Cuts at: 1872 2972 4714 1872
Size: 1100 1742 4545
Fragments arranged by size:
4545 1742 1100
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2326 2497 3559 5491 6392 6654
Size: 197 926 383 171 1062 1932 901 262
Cuts at: 6654 820
Size: 1553
Fragments arranged by size:
1932 1553 1062 926 901 383 262 197
171
SnaBI TAC'GTA
Cuts at: 493 5178 493
Size: 4685 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7387
SphI G_CATG'C
Cuts at: 1132 4745 1132
Size: 3613 3774
SspI AAT'ATT
Cuts at: 5 52 5311 5
Size: 47 5259 2081
Fragments arranged by size:
5259 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7387
StyI C'CwwG_G
Cuts at: 513 1065 2070 2112 2294 2315 2364 2659 2892
Size: 552 1005 42 182 21 49 295 233
Cuts at: 2892 4826 513
Size: 1934 3074
Fragments arranged by size:
3074 1934 1005 552 295 233 182 49
42 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3457 5414 5431 5584 5769 6214 325
Size: 1255 1877 1957 17 153 185 445 1498
Fragments arranged by size:
1957 1877 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5633 7345 96
Size: 274 80 33 51 1443 3656 1712 138
Fragments arranged by size:
3656 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7347 7379 7347
Size: 32 7355
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7387
XbaI T'CTAG_A
Cuts at: 4723 4723
Size: 7387
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3451 3451
Size: 7387
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7387
XmaI C'CCGG_G
Cuts at: 1870 2970 4712 1870
Size: 1100 1742 4545
Fragments arranged by size:
4545 1742 1100
XmnI GAAnn'nnTTC
Cuts at: 2636 5516 2636
Size: 2880 4507
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5195 276
Size: 53 83 186 936 3661 2468
Fragments arranged by size:
3661 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV
FalI FseI FspAI KasI NarI PacI PfoI PmlI
PpuMI PspXI PsrI RsrII SacII SanDI SfiI SfoI
SrfI SwaI