Restriction map for
pFN21AE2133
(Circular) (Six-base) MAPSORT of: pFN21AE2133.seq Check: 6074 from: 1 to: 7684
pFN21AE2133 7684 bp
With 182 enzymes: SgfI *
March 7, 2011 13:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5494 278
Size: 53 83 186 936 3958 2468
Fragments arranged by size:
3958 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5027 1937
Size: 3090 4594
Acc65I G'GTAC_C
Cuts at: 5005 5005
Size: 7684
AclI AA'CG_TT
Cuts at: 1563 5811 6184 1563
Size: 4248 373 3063
Fragments arranged by size:
4248 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4311 5747 7131 7399 1632
Size: 2679 1436 1384 268 1917
Fragments arranged by size:
2679 1917 1436 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7487
AflIII A'CryG_T
Cuts at: 1203 6583 7560 1203
Size: 5380 977 1327
Fragments arranged by size:
5380 1327 977
AgeI A'CCGG_T
Cuts at: 6515 6515
Size: 7684
AhdI GACnn_n'nnGTC
Cuts at: 6413 6413
Size: 7684
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7684
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7684
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3388 3420 3388
Size: 32 7652
AlwNI CAG_nnn'CTG
Cuts at: 5427 6999 5427
Size: 1572 6112
ApaLI G'TGCA_C
Cuts at: 4045 5741 6897 4045
Size: 1696 1156 4832
Fragments arranged by size:
4832 1696 1156
ApoI r'AATT_y
Cuts at: 1493 3829 4130 4204 4276 4403 4412 4993 5219
Size: 2336 301 74 72 127 9 581 226
Cuts at: 5219 5371 7480 1493
Size: 152 2109 1697
Fragments arranged by size:
2336 2109 1697 581 301 226 152 127
74 72 9
AseI AT'TA_AT
Cuts at: 160 1032 6238 160
Size: 872 5206 1606
Fragments arranged by size:
5206 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7684
AvaI C'yCGr_G
Cuts at: 1870 1943 2192 2879 4503 5009 1870
Size: 73 249 687 1624 506 4545
Fragments arranged by size:
4545 1624 687 506 249 73
BamHI G'GATC_C
Cuts at: 5014 5381 5014
Size: 367 7317
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5005 6460 618
Size: 325 217 627 3218 1455 1842
Fragments arranged by size:
3218 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2219 2853 5003 1391
Size: 828 634 2150 4072
Fragments arranged by size:
4072 2150 828 634
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2193 2518 928
Size: 971 294 325 6094
Fragments arranged by size:
6094 971 325 294
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3321 3355 5875 5909 1903
Size: 34 50 34 1300 34 2520 34 3678
Fragments arranged by size:
3678 2520 1300 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2975 3197 3365 5576 6786 2975
Size: 222 168 2211 1210 3873
Fragments arranged by size:
3873 2211 1210 222 168
BclI T'GATC_A
Cuts at: 1548 2100 1548
Size: 552 7132
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 6295 136
Size: 107 122 71 631 605 337 4286 1525
Fragments arranged by size:
4286 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 2089 2746 4363 7679 2089
Size: 657 1617 3316 2094
Fragments arranged by size:
3316 2094 1617 657
BlpI GC'TnA_GC
Cuts at: 5101 5101
Size: 7684
Bme1580I G_kGCm'C
Cuts at: 1790 4049 5745 6901 1790
Size: 2259 1696 1156 2573
Fragments arranged by size:
2573 2259 1696 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7684
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2723 3006 5415
Size: 416 53 83 399 24 1306 283 2409
Cuts at: 5415 6373 442
Size: 958 1753
Fragments arranged by size:
2409 1753 1306 958 416 399 283 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7684
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3519 3992 3519
Size: 473 7211
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2439 3582 6344 1239
Size: 1200 1143 2762 2579
Fragments arranged by size:
2762 2579 1200 1143
Bpu10I CC'TnA_GC
Cuts at: 2218 4968 2218
Size: 2750 4934
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3306 3974 4777 5809 6674 6972 3306
Size: 668 803 1032 865 298 4018
Fragments arranged by size:
4018 1032 865 803 668 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2200 4428 6347 882
Size: 617 701 2228 1919 2219
Fragments arranged by size:
2228 2219 1919 701 617
BsaAI yAC'GTr
Cuts at: 493 1204 3150 5475 493
Size: 711 1946 2325 2702
Fragments arranged by size:
2702 2325 1946 711
BsaBI GATnn'nnATC
Cuts at: 2751 7678 2751
Size: 4927 2757
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2544 3353 5491 5873
Size: 53 83 186 936 1011 809 2138 382
Cuts at: 5873 6508 275
Size: 635 1451
Fragments arranged by size:
2138 1451 1011 936 809 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2978 4681 6117 6515 6789 6936 7347 2978
Size: 1703 1436 398 274 147 411 3315
Fragments arranged by size:
3315 1703 1436 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7459
Fragments arranged by size:
7459 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2388 2751 2897 3650 3745 4349 1186
Size: 1202 363 146 753 95 604 4521
Fragments arranged by size:
4521 1202 753 604 363 146 95
BseYI C'CCAG_C
Cuts at: 4002 4006 4036 4779 6887 7383 4002
Size: 4 30 743 2108 496 4303
Fragments arranged by size:
4303 2108 743 496 30 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3409 1163
Size: 573 1673 5438
Fragments arranged by size:
5438 1673 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2583 5134 5895 6923 1540
Size: 275 186 582 2551 761 1028 2301
Fragments arranged by size:
2551 2301 1028 761 582 275 186
BsiHKAI G_wGCw'C
Cuts at: 2219 3330 4049 5745 5830 6901 2219
Size: 1111 719 1696 85 1071 3002
Fragments arranged by size:
3002 1696 1111 1071 719 85
BsmI GAATG_Cn'
Cuts at: 2729 4677 5195 5288 2729
Size: 1948 518 93 5125
Fragments arranged by size:
5125 1948 518 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3572 3572
Size: 7684
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2219 2853 3330 4049 5003 5745 5830
Size: 399 429 634 477 719 954 742 85
Cuts at: 5830 6901 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 954 742 719 634 477 429
399 85
BspEI T'CCGG_A
Cuts at: 7347 7347
Size: 7684
BspHI T'CATG_A
Cuts at: 2668 4512 5573 7303 2668
Size: 1844 1061 1730 3049
Fragments arranged by size:
3049 1844 1730 1061
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4041 5039 844
Size: 3197 998 3489
Fragments arranged by size:
3489 3197 998
BsrBI CCG'CTC
Cuts at: 1394 1823 5571 1394
Size: 429 3748 3507
Fragments arranged by size:
3748 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2067 2771 4549 6179 6353 65
Size: 1183 819 704 1778 1630 174 1396
Fragments arranged by size:
1778 1630 1396 1183 819 704 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2008 3749 6328 6515 1700
Size: 110 198 1741 2579 187 2869
Fragments arranged by size:
2869 2579 1741 198 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7684
BssHII G'CGCG_C
Cuts at: 1925 2018 1925
Size: 93 7591
BssSI C'ACGA_G
Cuts at: 4016 5744 6756 4016
Size: 1728 1012 4944
Fragments arranged by size:
4944 1728 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2724 794
Size: 1930 5754
BstBI TT'CG_AA
Cuts at: 6488 6488
Size: 7684
BstEII G'GTnAC_C
Cuts at: 1184 3028 3745 1184
Size: 1844 717 5123
Fragments arranged by size:
5123 1844 717
BstXI CCAn_nnnn'nTGG
Cuts at: 4399 4399
Size: 7684
BstYI r'GATC_y
Cuts at: 1972 2089 2746 4363 5014 5381 5766 5783 7235
Size: 117 657 1617 651 367 385 17 1452
Cuts at: 7235 7321 7333 7679 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1617 1452 657 651 385 367 346
117 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2166 2385 2839 4465 513
Size: 552 1101 219 454 1626 3732
Fragments arranged by size:
3732 1626 1101 552 454 219
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4877 487
Size: 683 56 3651 3294
Fragments arranged by size:
3651 3294 683 56
BtsI GCAGTG_nn'
Cuts at: 2273 5200 5994 6014 2273
Size: 2927 794 20 3943
Fragments arranged by size:
3943 2927 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7684
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7649
DraI TTT'AAA
Cuts at: 3124 3591 4723 4988 3124
Size: 467 1132 265 5820
Fragments arranged by size:
5820 1132 467 265
DraIII CAC_nnn'GTG
Cuts at: 4658 4658
Size: 7684
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2549 6691 809
Size: 507 1233 4142 1802
Fragments arranged by size:
4142 1802 1233 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2029 4440 4802
Size: 54 1425 50 275 18 199 2411 362
Cuts at: 4802 4882 5131 6020 7649 8
Size: 80 249 889 1629 43
Fragments arranged by size:
2411 1629 1425 889 362 275 249 199
80 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5131 1537
Size: 275 3319 4090
Fragments arranged by size:
4090 3319 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3004 3093 3426 3668 4326 5614 1353
Size: 1651 89 333 242 658 1288 3423
Fragments arranged by size:
3423 1651 1288 658 333 242 89
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6271 6645 6791 387
Size: 1064 138 4682 374 146 1280
Fragments arranged by size:
4682 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2217 2217
Size: 7684
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2439 3582 4311 5747 6344 7131 7399
Size: 393 807 1143 729 1436 597 787 268
Cuts at: 7399 1239
Size: 1524
Fragments arranged by size:
1524 1436 1143 807 787 729 597 393
268
EcoO109I rG'GnC_Cy
Cuts at: 3602 3602
Size: 7684
EcoRI G'AATT_C
Cuts at: 4276 4993 5371 4276
Size: 717 378 6589
Fragments arranged by size:
6589 717 378
EcoRV GAT'ATC
Cuts at: 4510 4510
Size: 7684
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2532 4385 2532
Size: 1853 5831
FspI TGC'GCA
Cuts at: 6190 6190
Size: 7684
HaeII r_GCGC'y
Cuts at: 1678 6831 1678
Size: 5153 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2835 2867 3145 3177 3614
Size: 32 135 32 1263 32 278 32 437
Cuts at: 3614 3646 3903 3935 4244 4276 4604 4636 6325
Size: 32 257 32 309 32 328 32 1689
Cuts at: 6325 6357 6399 6431 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1689 1263 437 328 309 278 257
135 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5028 5274 669
Size: 988 281 3090 246 3079
Fragments arranged by size:
3090 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7684
HpaI GTT'AAC
Cuts at: 5274 5274
Size: 7684
KpnI G_GTAC'C
Cuts at: 5009 5009
Size: 7684
MfeI C'AATT_G
Cuts at: 3645 5283 3645
Size: 1638 6046
MluI A'CGCG_T
Cuts at: 7560 7560
Size: 7684
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4548 6798 6982 1305
Size: 3243 2250 184 2007
Fragments arranged by size:
3243 2250 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5862
Fragments arranged by size:
5862 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4766 5642 6001 6160 518
Size: 648 3600 876 359 159 2042
Fragments arranged by size:
3600 2042 876 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2035 2041 2243 2639 4006 4843 5100 5777
Size: 429 6 202 396 1367 837 257 677
Cuts at: 5777 6925 7170 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1367 1148 837 677 429 396 257
245 202 6
NaeI GCC'GGC
Cuts at: 2010 2010
Size: 7684
NcoI C'CATG_G
Cuts at: 513 1065 2166 2385 2839 513
Size: 552 1101 219 454 5358
Fragments arranged by size:
5358 1101 552 454 219
NdeI CA'TA_TG
Cuts at: 387 4624 387
Size: 4237 3447
NgoMIV G'CCGG_C
Cuts at: 2008 2008
Size: 7684
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7684
NotI GC'GGCC_GC
Cuts at: 5131 5131
Size: 7684
NspI r_CATG'y
Cuts at: 1132 1229 4216 5042 6587 1132
Size: 97 2987 826 1545 2229
Fragments arranged by size:
2987 2229 1545 826 97
PasI CC'CwG_GG
Cuts at: 1348 2297 1348
Size: 949 6735
PciI A'CATG_T
Cuts at: 6583 6583
Size: 7684
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7684
PmeI GTTT'AAAC
Cuts at: 4988 4988
Size: 7684
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5753 5785 7266 7298 5753
Size: 32 1481 32 6139
Fragments arranged by size:
6139 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7684
PsiI TTA'TAA
Cuts at: 3053 4766 5254 3053
Size: 1713 488 5483
Fragments arranged by size:
5483 1713 488
PspXI vC'TCGA_Gb
Cuts at: 2879 2879
Size: 7684
PstI C_TGCA'G
Cuts at: 830 5036 830
Size: 4206 3478
PvuI CG_AT'CG
Cuts at: 2001 2583 2001
Size: 582 7102
PvuII CAG'CTG
Cuts at: 2035 2639 4006 4843 2035
Size: 604 1367 837 4876
Fragments arranged by size:
4876 1367 837 604
SacI G_AGCT'C
Cuts at: 2219 2219
Size: 7684
SalI G'TCGA_C
Cuts at: 1936 5026 1936
Size: 3090 4594
SbfI CC_TGCA'GG
Cuts at: 5036 5036
Size: 7684
ScaI AGT'ACT
Cuts at: 3502 3770 5932 7644 3502
Size: 268 2162 1712 3542
Fragments arranged by size:
3542 2162 1712 268
SfcI C'TryA_G
Cuts at: 826 1045 3731 4746 5032 6167 6848 7039 826
Size: 219 2686 1015 286 1135 681 191 1471
Fragments arranged by size:
2686 1471 1135 1015 681 286 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7684
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7684
SmaI CCC'GGG
Cuts at: 1872 5011 1872
Size: 3139 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2879 3321 3989 4503 4792 5788
Size: 197 926 936 442 668 514 289 996
Cuts at: 5788 6689 6951 820
Size: 901 262 1553
Fragments arranged by size:
1553 996 936 926 901 668 514 442
289 262 197
SnaBI TAC'GTA
Cuts at: 493 5475 493
Size: 4982 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7684
SphI G_CATG'C
Cuts at: 1132 4216 5042 1132
Size: 3084 826 3774
Fragments arranged by size:
3774 3084 826
SspI AAT'ATT
Cuts at: 5 52 2466 4024 4632 5608 5
Size: 47 2414 1558 608 976 2081
Fragments arranged by size:
2414 2081 1558 976 608 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7684
StyI C'CwwG_G
Cuts at: 513 1065 2166 2385 2839 3562 5123 513
Size: 552 1101 219 454 723 1561 3074
Fragments arranged by size:
3074 1561 1101 723 552 454 219
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2581 3478 4478 5711 5728 5881 6066
Size: 1255 1001 897 1000 1233 17 153 185
Cuts at: 6066 6511 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1233 1001 1000 897 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3500 3699 3768
Size: 274 80 33 51 1443 1523 199 69
Cuts at: 3768 5930 7642 96
Size: 2162 1712 138
Fragments arranged by size:
2162 1712 1523 1443 274 199 138 80
69 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2678 2710 3474 3506 7644 7676 2678
Size: 32 764 32 4138 32 2686
Fragments arranged by size:
4138 2686 764 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7684
XbaI T'CTAG_A
Cuts at: 5020 5020
Size: 7684
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2710 3384 3390 2011
Size: 699 674 6 6305
Fragments arranged by size:
6305 699 674 6
XhoI C'TCGA_G
Cuts at: 1943 2879 4503 1943
Size: 936 1624 5124
Fragments arranged by size:
5124 1624 936
XmaI C'CCGG_G
Cuts at: 1870 5009 1870
Size: 3139 4545
XmnI GAAnn'nnTTC
Cuts at: 4131 4365 5813 4131
Size: 234 1448 6002
Fragments arranged by size:
6002 1448 234
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5492 276
Size: 53 83 186 936 3958 2468
Fragments arranged by size:
3958 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BstZ17I Bsu36I EcoNI FseI FspAI KasI
NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI