Restriction map for
pFN21AE2132
(Circular) (Six-base) MAPSORT of: pFN21AE2132.seq Check: 7920 from: 1 to: 7294
pFN21AE2132 7294 bp
With 182 enzymes: SgfI *
August 16, 2010 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5104 278
Size: 53 83 186 936 3568 2468
Fragments arranged by size:
3568 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2021 3237 4149 4637 1937
Size: 84 1216 912 488 4594
Fragments arranged by size:
4594 1216 912 488 84
Acc65I G'GTAC_C
Cuts at: 3542 4615 3542
Size: 1073 6221
AclI AA'CG_TT
Cuts at: 1563 5421 5794 1563
Size: 3858 373 3063
Fragments arranged by size:
3858 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2220 2498 3050 3378 3434 4331 4431 5357
Size: 588 278 552 328 56 897 100 926
Cuts at: 5357 6741 7009 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 926 897 588 552 328 278
268 100 56
AflII C'TTAA_G
Cuts at: 820 1017 3350 820
Size: 197 2333 4764
Fragments arranged by size:
4764 2333 197
AflIII A'CryG_T
Cuts at: 1203 3337 3570 3600 6193 7170 1203
Size: 2134 233 30 2593 977 1327
Fragments arranged by size:
2593 2134 1327 977 233 30
AgeI A'CCGG_T
Cuts at: 6125 6125
Size: 7294
AhdI GACnn_n'nnGTC
Cuts at: 6023 6023
Size: 7294
AleI CACnn'nnGTG
Cuts at: 1166 2336 1166
Size: 1170 6124
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2205 3702 4115 1184
Size: 1021 1497 413 4363
Fragments arranged by size:
4363 1497 1021 413
AlwNI CAG_nnn'CTG
Cuts at: 3562 3790 4571 5037 6609 3562
Size: 228 781 466 1572 4247
Fragments arranged by size:
4247 1572 781 466 228
ApaLI G'TGCA_C
Cuts at: 5351 6507 5351
Size: 1156 6138
ApoI r'AATT_y
Cuts at: 1493 2665 3441 3500 4603 4829 4981 7090 1493
Size: 1172 776 59 1103 226 152 2109 1697
Fragments arranged by size:
2109 1697 1172 1103 776 226 152 59
AseI AT'TA_AT
Cuts at: 160 1032 2604 4214 5848 160
Size: 872 1572 1610 1634 1606
Fragments arranged by size:
1634 1610 1606 1572 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7294
AvaI C'yCGr_G
Cuts at: 1870 1943 2026 2295 2894 2943 4619 1870
Size: 73 83 269 599 49 1676 4545
Fragments arranged by size:
4545 1676 599 269 83 73 49
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3526 3559 3526
Size: 33 7261
BamHI G'GATC_C
Cuts at: 4624 4991 4624
Size: 367 6927
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3542 4615 6070 618
Size: 325 217 627 1755 1073 1455 1842
Fragments arranged by size:
1842 1755 1455 1073 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2189 2363 2979 3617 4047 4613 1391
Size: 798 174 616 638 430 566 4072
Fragments arranged by size:
4072 798 638 616 566 430 174
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2810 2841 3087 4583 928
Size: 971 911 31 246 1496 3639
Fragments arranged by size:
3639 1496 971 911 246 31
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5485 5519 1903
Size: 34 3548 34 3678
Fragments arranged by size:
3678 3548 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5186 6396 5186
Size: 1210 6084
BclI T'GATC_A
Cuts at: 1548 2871 3057 1548
Size: 1323 186 5785
Fragments arranged by size:
5785 1323 186
BfrBI ATG'CAT
Cuts at: 2230 2230
Size: 7294
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2037 3094 4127
Size: 107 122 71 631 605 365 1057 1033
Cuts at: 4127 5905 136
Size: 1778 1525
Fragments arranged by size:
1778 1525 1057 1033 631 605 365 122
107 71
BglII A'GATC_T
Cuts at: 7289 7289
Size: 7294
BlpI GC'TnA_GC
Cuts at: 4222 4711 4222
Size: 489 6805
Bme1580I G_kGCm'C
Cuts at: 1790 2333 2935 4543 5355 6511 1790
Size: 543 602 1608 812 1156 2573
Fragments arranged by size:
2573 1608 1156 812 602 543
BmgBI CAC'GTC
Cuts at: 1318 2203 3603 1318
Size: 885 1400 5009
Fragments arranged by size:
5009 1400 885
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2973 4581 5025
Size: 416 53 83 399 24 1556 1608 444
Cuts at: 5025 5983 442
Size: 958 1753
Fragments arranged by size:
1753 1608 1556 958 444 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7294
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2190 3601 5954 1239
Size: 951 1411 2353 2579
Fragments arranged by size:
2579 2353 1411 951
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5419 6284 6582 5419
Size: 865 298 6131
Fragments arranged by size:
6131 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5957 882
Size: 617 4458 2219
Fragments arranged by size:
4458 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3571 3643 5085 493
Size: 711 2367 72 1442 2702
Fragments arranged by size:
2702 2367 1442 711 72
BsaBI GATnn'nnATC
Cuts at: 3518 7288 3518
Size: 3770 3524
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2018 2072 3985 5101
Size: 53 83 186 936 485 54 1913 1116
Cuts at: 5101 5483 6118 275
Size: 382 635 1451
Fragments arranged by size:
1913 1451 1116 936 635 485 382 186
83 54 53
BsaWI w'CCGG_w
Cuts at: 3076 5727 6125 6399 6546 6957 3076
Size: 2651 398 274 147 411 3413
Fragments arranged by size:
3413 2651 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3017 3047 1178
Size: 30 165 30 1614 30 5425
Fragments arranged by size:
5425 1614 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4040 4052 4055 1186
Size: 2854 12 3 4425
Fragments arranged by size:
4425 2854 12 3
BseYI C'CCAG_C
Cuts at: 2288 2883 3555 3806 6497 6993 2288
Size: 595 672 251 2691 496 2589
Fragments arranged by size:
2691 2589 672 595 496 251
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2349 3642 1163
Size: 573 613 1293 4815
Fragments arranged by size:
4815 1293 613 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2965 4744 5505 6533 1540
Size: 275 186 964 1779 761 1028 2301
Fragments arranged by size:
2301 1779 1028 964 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3691 4047 5355 5440 6511 3691
Size: 356 1308 85 1071 4474
Fragments arranged by size:
4474 1308 1071 356 85
BsiWI C'GTAC_G
Cuts at: 3639 3639
Size: 7294
BsmI GAATG_Cn'
Cuts at: 2230 4805 4898 2230
Size: 2575 93 4626
Fragments arranged by size:
4626 2575 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2104 2104
Size: 7294
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2189 2333 2363 2935 2979 3617 3691
Size: 399 399 144 30 572 44 638 74
Cuts at: 3691 4047 4543 4613 5355 5440 6511 1391
Size: 356 496 70 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 638 572 496 399 399
356 144 85 74 70 44 30
BspEI T'CCGG_A
Cuts at: 3076 6957 3076
Size: 3881 3413
BspHI T'CATG_A
Cuts at: 5183 6913 5183
Size: 1730 5564
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2187 4649 844
Size: 1343 2462 3489
Fragments arranged by size:
3489 2462 1343
BsrBI CCG'CTC
Cuts at: 1394 1823 3033 5181 1394
Size: 429 1210 2148 3507
Fragments arranged by size:
3507 2148 1210 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2967 3795 4074 5789 5963 65
Size: 1183 1719 828 279 1715 174 1396
Fragments arranged by size:
1719 1715 1396 1183 828 279 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2043 5938 6125 1700
Size: 110 233 3895 187 2869
Fragments arranged by size:
3895 2869 233 187 110
BsrGI T'GTAC_A
Cuts at: 96 2670 3573 96
Size: 2574 903 3817
Fragments arranged by size:
3817 2574 903
BssHII G'CGCG_C
Cuts at: 1925 3830 1925
Size: 1905 5389
BssSI C'ACGA_G
Cuts at: 5354 6366 5354
Size: 1012 6282
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2155 2203 4538 794
Size: 1361 48 2335 3550
Fragments arranged by size:
3550 2335 1361 48
BstBI TT'CG_AA
Cuts at: 6098 6098
Size: 7294
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7294
BstXI CCAn_nnnn'nTGG
Cuts at: 4276 4276
Size: 7294
BstYI r'GATC_y
Cuts at: 1972 2386 4624 4991 5376 5393 6845 6931 6943
Size: 414 2238 367 385 17 1452 86 12
Cuts at: 6943 7289 1972
Size: 346 1977
Fragments arranged by size:
2238 1977 1452 414 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3963 3963
Size: 7294
BtgI C'CryG_G
Cuts at: 513 1065 2035 2136 2959 4103 513
Size: 552 970 101 823 1144 3704
Fragments arranged by size:
3704 1144 970 823 552 101
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6555
Fragments arranged by size:
6555 683 56
BtsI GCAGTG_nn'
Cuts at: 3633 4532 4810 5604 5624 3633
Size: 899 278 794 20 5303
Fragments arranged by size:
5303 899 794 278 20
ClaI AT'CG_AT
Cuts at: 1554 3723 1554
Size: 2169 5125
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7259
DraI TTT'AAA
Cuts at: 2628 4598 2628
Size: 1970 5324
DraIII CAC_nnn'GTG
Cuts at: 4538 4538
Size: 7294
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2723 6301 809
Size: 507 1407 3578 1802
Fragments arranged by size:
3578 1802 1407 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2962 4741 5630
Size: 54 1425 50 275 18 1132 1779 889
Cuts at: 5630 7259 8
Size: 1629 43
Fragments arranged by size:
1779 1629 1425 1132 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2962 4741 1537
Size: 275 1150 1779 4090
Fragments arranged by size:
4090 1779 1150 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3044 4275 5224 1353
Size: 1691 1231 949 3423
Fragments arranged by size:
3423 1691 1231 949
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5881 6255 6401 387
Size: 1064 138 4292 374 146 1280
Fragments arranged by size:
4292 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4045 4045
Size: 7294
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2190 2220 2498 3050 3378 3434 3601
Size: 393 558 30 278 552 328 56 167
Cuts at: 3601 4331 4431 5357 5954 6741 7009 1239
Size: 730 100 926 597 787 268 1524
Fragments arranged by size:
1524 926 787 730 597 558 552 393
328 278 268 167 100 56 30
EcoNI CCTnn'n_nnAGG
Cuts at: 3979 3979
Size: 7294
EcoO109I rG'GnC_Cy
Cuts at: 2374 2374
Size: 7294
EcoRI G'AATT_C
Cuts at: 4603 4981 4603
Size: 378 6916
FspI TGC'GCA
Cuts at: 2196 5800 2196
Size: 3604 3690
HaeII r_GCGC'y
Cuts at: 1678 2995 6441 1678
Size: 1317 3446 2531
Fragments arranged by size:
3446 2531 1317
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2078 2110 2117 2149 2345
Size: 32 135 32 506 32 7 32 196
Cuts at: 2345 2377 2378 2410 2667 2699 3673 3705 3802
Size: 32 1 32 257 32 974 32 97
Cuts at: 3802 3834 5935 5967 6009 6041 1373
Size: 32 2101 32 42 32 2626
Fragments arranged by size:
2626 2101 974 506 257 196 135 97
42 32 32 32 32 32 32 32
32 32 32 32 7 1
HincII GTy'rAC
Cuts at: 669 1657 1938 2022 3142 4638 4884 669
Size: 988 281 84 1120 1496 246 3079
Fragments arranged by size:
3079 1496 1120 988 281 246 84
HindIII A'AGCT_T
Cuts at: 748 3062 4424 748
Size: 2314 1362 3618
Fragments arranged by size:
3618 2314 1362
HpaI GTT'AAC
Cuts at: 4884 4884
Size: 7294
KpnI G_GTAC'C
Cuts at: 3546 4619 3546
Size: 1073 6221
MfeI C'AATT_G
Cuts at: 4893 4893
Size: 7294
MluI A'CGCG_T
Cuts at: 7170 7170
Size: 7294
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2732 6408 6592 1305
Size: 1427 3676 184 2007
Fragments arranged by size:
3676 2007 1427 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5472
Fragments arranged by size:
5472 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2336 3252 4058 4097 4274 5252 5611
Size: 648 1170 916 806 39 177 978 359
Cuts at: 5611 5770 518
Size: 159 2042
Fragments arranged by size:
2042 1170 978 916 806 648 359 177
159 39
MspA1I CmG'CkG
Cuts at: 1606 2037 2862 2887 2961 3559 3670 3910 4105
Size: 431 825 25 74 598 111 240 195
Cuts at: 4105 4710 5387 6535 6780 1606
Size: 605 677 1148 245 2120
Fragments arranged by size:
2120 1148 825 677 605 598 431 245
240 195 111 74 25
NaeI GCC'GGC
Cuts at: 2045 2045
Size: 7294
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6742
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7294
NgoMIV G'CCGG_C
Cuts at: 2043 2043
Size: 7294
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7294
NotI GC'GGCC_GC
Cuts at: 2962 4741 2962
Size: 1779 5515
NsiI A_TGCA'T
Cuts at: 2232 2232
Size: 7294
NspI r_CATG'y
Cuts at: 1132 1229 2490 2587 3088 4652 6197 1132
Size: 97 1261 97 501 1564 1545 2229
Fragments arranged by size:
2229 1564 1545 1261 501 97 97
PasI CC'CwG_GG
Cuts at: 1348 3588 1348
Size: 2240 5054
PciI A'CATG_T
Cuts at: 6193 6193
Size: 7294
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2318 3013 1108
Size: 1210 695 5389
Fragments arranged by size:
5389 1210 695
PfoI T'CCnGG_A
Cuts at: 3362 4047 3362
Size: 685 6609
PmeI GTTT'AAAC
Cuts at: 4598 4598
Size: 7294
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5363 5395 6876 6908 5363
Size: 32 1481 32 5749
Fragments arranged by size:
5749 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3984 1613
Size: 2371 4923
PsiI TTA'TAA
Cuts at: 4864 4864
Size: 7294
PstI C_TGCA'G
Cuts at: 830 2184 2394 3094 3567 3681 4646 830
Size: 1354 210 700 473 114 965 3478
Fragments arranged by size:
3478 1354 965 700 473 210 114
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7294
PvuII CAG'CTG
Cuts at: 2887 3559 3670 3910 2887
Size: 672 111 240 6271
Fragments arranged by size:
6271 672 240 111
SacI G_AGCT'C
Cuts at: 4047 4047
Size: 7294
SacII CC_GC'GG
Cuts at: 2038 2962 4106 2038
Size: 924 1144 5226
Fragments arranged by size:
5226 1144 924
SalI G'TCGA_C
Cuts at: 1936 2020 4636 1936
Size: 84 2616 4594
Fragments arranged by size:
4594 2616 84
SbfI CC_TGCA'GG
Cuts at: 3094 4646 3094
Size: 1552 5742
ScaI AGT'ACT
Cuts at: 5542 7254 5542
Size: 1712 5582
SexAI A'CCwGG_T
Cuts at: 3999 3999
Size: 7294
SfcI C'TryA_G
Cuts at: 826 1045 2180 2390 3090 3563 3677 4642 5777
Size: 219 1135 210 700 473 114 965 1135
Cuts at: 5777 6458 6649 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1135 965 700 681 473 219
210 191 114
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7294
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7294
SmaI CCC'GGG
Cuts at: 1872 2945 4621 1872
Size: 1073 1676 4545
Fragments arranged by size:
4545 1676 1073
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3350 5398 6299 6561 820
Size: 197 926 1407 2048 901 262 1553
Fragments arranged by size:
2048 1553 1407 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3643 5085 493
Size: 3150 1442 2702
Fragments arranged by size:
3150 2702 1442
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7294
SphI G_CATG'C
Cuts at: 1132 2490 4652 1132
Size: 1358 2162 3774
Fragments arranged by size:
3774 2162 1358
SspI AAT'ATT
Cuts at: 5 52 5218 5
Size: 47 5166 2081
Fragments arranged by size:
5166 2081 47
StuI AGG'CCT
Cuts at: 1517 2350 2909 1517
Size: 833 559 5902
Fragments arranged by size:
5902 833 559
StyI C'CwwG_G
Cuts at: 513 1065 3399 4733 513
Size: 552 2334 1334 3074
Fragments arranged by size:
3074 2334 1334 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3020 5321 5338 5491 5676 6121 325
Size: 1255 1440 2301 17 153 185 445 1498
Fragments arranged by size:
2301 1498 1440 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2670 3573 3597
Size: 274 80 33 51 1443 693 903 24
Cuts at: 3597 3749 4011 5540 7252 96
Size: 152 262 1529 1712 138
Fragments arranged by size:
1712 1529 1443 903 693 274 262 152
138 80 51 33 24
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3017 3049 7254 7286 3017
Size: 32 4205 32 3025
Fragments arranged by size:
4205 3025 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3146 3790 1316
Size: 1830 644 4820
Fragments arranged by size:
4820 1830 644
XbaI T'CTAG_A
Cuts at: 4630 4630
Size: 7294
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2863 3010 3602 2863
Size: 147 592 6555
Fragments arranged by size:
6555 592 147
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7294
XmaI C'CCGG_G
Cuts at: 1870 2943 4619 1870
Size: 1073 1676 4545
Fragments arranged by size:
4545 1676 1073
XmnI GAAnn'nnTTC
Cuts at: 2499 2703 4183 4482 5423 2499
Size: 204 1480 299 941 4370
Fragments arranged by size:
4370 1480 941 299 204
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5102 276
Size: 53 83 186 936 3568 2468
Fragments arranged by size:
3568 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SacI SacII SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BplI Bpu10I BstZ17I EcoRV FalI FseI FspAI KasI
NarI NruI PacI PmlI PpuMI PspOMI PspXI PsrI
RsrII SanDI SapI SfiI SfoI SrfI SwaI