Restriction map for
pFN21AE2123
(Circular) (Six-base) MAPSORT of: pFN21AE2123.seq Check: 3122 from: 1 to: 7447
pFN21AE2123 7447 bp
With 182 enzymes: SgfI *
February 3, 2015 17:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3353 4378 3353
Size: 1025 6422
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5257 278
Size: 53 83 186 936 3721 2468
Fragments arranged by size:
3721 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4790 1937
Size: 2853 4594
Acc65I G'GTAC_C
Cuts at: 4768 4768
Size: 7447
AclI AA'CG_TT
Cuts at: 1563 5574 5947 1563
Size: 4011 373 3063
Fragments arranged by size:
4011 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3515 4598 4664 5510 6894 7162 1632
Size: 1883 1083 66 846 1384 268 1917
Fragments arranged by size:
1917 1883 1384 1083 846 268 66
AfeI AGC'GCT
Cuts at: 2288 4004 4334 2288
Size: 1716 330 5401
Fragments arranged by size:
5401 1716 330
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7250
AflIII A'CryG_T
Cuts at: 1203 4440 6346 7323 1203
Size: 3237 1906 977 1327
Fragments arranged by size:
3237 1906 1327 977
AgeI A'CCGG_T
Cuts at: 6278 6278
Size: 7447
AhdI GACnn_n'nnGTC
Cuts at: 6176 6176
Size: 7447
AleI CACnn'nnGTG
Cuts at: 1166 4307 1166
Size: 3141 4306
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7447
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2468 2500 4199 4231 2468
Size: 32 1699 32 5684
Fragments arranged by size:
5684 1699 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2473 2777 3547 5190 6762 2473
Size: 304 770 1643 1572 3158
Fragments arranged by size:
3158 1643 1572 770 304
ApaLI G'TGCA_C
Cuts at: 4130 5504 6660 4130
Size: 1374 1156 4917
Fragments arranged by size:
4917 1374 1156
ApoI r'AATT_y
Cuts at: 1493 4756 4982 5134 7243 1493
Size: 3263 226 152 2109 1697
Fragments arranged by size:
3263 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6001 160
Size: 872 4969 1606
Fragments arranged by size:
4969 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7447
AvaI C'yCGr_G
Cuts at: 1870 1943 2294 2822 2844 3350 4772 1870
Size: 73 351 528 22 506 1422 4545
Fragments arranged by size:
4545 1422 528 506 351 73 22
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4387 4420 4387
Size: 33 7414
BamHI G'GATC_C
Cuts at: 3028 3426 4777 5144 3028
Size: 398 1351 367 5331
Fragments arranged by size:
5331 1351 398 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2048 2389 2609 3220 3283
Size: 325 217 627 261 341 220 611 63
Cuts at: 3283 3611 3689 4768 6223 618
Size: 328 78 1079 1455 1842
Fragments arranged by size:
1842 1455 1079 627 611 341 328 325
261 220 217 78 63
BanII G_rGCy'C
Cuts at: 1391 2072 2192 2823 2949 3242 3655 3945 4038
Size: 681 120 631 126 293 413 290 93
Cuts at: 4038 4350 4766 1391
Size: 312 416 4072
Fragments arranged by size:
4072 681 631 416 413 312 293 290
126 120 93
BbeI G_GCGC'C
Cuts at: 2052 3287 2052
Size: 1235 6212
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3506 3516 4594 928
Size: 971 1607 10 1078 3781
Fragments arranged by size:
3781 1607 1078 971 10
BbvCI CC'TCA_GC
Cuts at: 4097 4291 4097
Size: 194 7253
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5638 5672 1903
Size: 34 3701 34 3678
Fragments arranged by size:
3701 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5339 6549 5339
Size: 1210 6237
BclI T'GATC_A
Cuts at: 1548 2217 1548
Size: 669 6778
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2030 2516 3097
Size: 107 122 71 631 605 358 486 581
Cuts at: 3097 6058 136
Size: 2961 1525
Fragments arranged by size:
2961 1525 631 605 581 486 358 122
107 71
BglII A'GATC_T
Cuts at: 3166 3555 7442 3166
Size: 389 3887 3171
Fragments arranged by size:
3887 3171 389
BlpI GC'TnA_GC
Cuts at: 3902 4124 4264 4864 3902
Size: 222 140 600 6485
Fragments arranged by size:
6485 600 222 140
Bme1580I G_kGCm'C
Cuts at: 1790 2612 2728 3225 3614 3692 3710 4134 5508
Size: 822 116 497 389 78 18 424 1374
Cuts at: 5508 6664 1790
Size: 1156 2573
Fragments arranged by size:
2573 1374 1156 822 497 424 389 116
78 18
BmgBI CAC'GTC
Cuts at: 1318 3504 4159 4426 1318
Size: 2186 655 267 4339
Fragments arranged by size:
4339 2186 655 267
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2852 3304 5178
Size: 416 53 83 399 24 1435 452 1874
Cuts at: 5178 6136 442
Size: 958 1753
Fragments arranged by size:
1874 1753 1435 958 452 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2831 1056
Size: 1775 5672
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3577 3631 3577
Size: 54 7393
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2129 2387 2735 2768 2799 2853 2889 2913
Size: 890 258 348 33 31 54 36 24
Cuts at: 2913 2933 2943 2973 3003 3051 3077 3147 3653
Size: 20 10 30 30 48 26 70 506
Cuts at: 3653 3959 4027 4255 4342 4538 6107 1239
Size: 306 68 228 87 196 1569 2579
Fragments arranged by size:
2579 1569 890 506 348 306 258 228
196 87 70 68 54 48 36 33
31 30 30 26 24 20 10
Bpu10I CC'TnA_GC
Cuts at: 4079 4097 4291 4079
Size: 18 194 7235
Fragments arranged by size:
7235 194 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2651 3416 4709 5572 6437 6735 2651
Size: 765 1293 863 865 298 3363
Fragments arranged by size:
3363 1293 865 863 765 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4241 6110 882
Size: 617 2742 1869 2219
Fragments arranged by size:
2742 2219 1869 617
BsaAI yAC'GTr
Cuts at: 493 1204 2686 5238 493
Size: 711 1482 2552 2702
Fragments arranged by size:
2702 2552 1482 711
BsaBI GATnn'nnATC
Cuts at: 4172 7441 4172
Size: 3269 4178
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2049 2097 2118 2508
Size: 53 83 186 936 516 48 21 390
Cuts at: 2508 3284 5254 5636 6271 275
Size: 776 1970 382 635 1451
Fragments arranged by size:
1970 1451 936 776 635 516 390 382
186 83 53 48 21
BsaWI w'CCGG_w
Cuts at: 2650 2941 3531 4569 5880 6278 6552 6699 7110
Size: 291 590 1038 1311 398 274 147 411
Cuts at: 7110 2650
Size: 2987
Fragments arranged by size:
2987 1311 1038 590 411 398 291 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2225 2255 2887 2917 4346
Size: 30 165 30 822 30 632 30 1429
Cuts at: 4346 4376 4659 4689 1178
Size: 30 283 30 3936
Fragments arranged by size:
3936 1429 822 632 283 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2225 2512 2587 3149 3802 4135 4178 4279
Size: 1039 287 75 562 653 333 43 101
Cuts at: 4279 4535 4538 4541 1186
Size: 256 3 3 4092
Fragments arranged by size:
4092 1039 653 562 333 287 256 101
75 43 3 3
BseYI C'CCAG_C
Cuts at: 2742 2852 3252 4025 6650 7146 2742
Size: 110 400 773 2625 496 3043
Fragments arranged by size:
3043 2625 773 496 400 110
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2030 2129 2246 2276 2708 1163
Size: 573 294 99 117 30 432 5902
Fragments arranged by size:
5902 573 432 294 117 99 30
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2034 3665 4015 4140 4897 5658
Size: 275 186 33 1631 350 125 757 761
Cuts at: 5658 6686 1540
Size: 1028 2301
Fragments arranged by size:
2301 1631 1028 761 757 350 275 186
125 33
BsiHKAI G_wGCw'C
Cuts at: 2192 2385 2754 2823 2851 2949 3279 3399 4038
Size: 193 369 69 28 98 330 120 639
Cuts at: 4038 4134 5508 5593 6664 2192
Size: 96 1374 85 1071 2975
Fragments arranged by size:
2975 1374 1071 639 369 330 193 120
98 96 85 69 28
BsmI GAATG_Cn'
Cuts at: 4958 5051 4958
Size: 93 7354
BsmBI CGTCTCn'nnnn_
Cuts at: 3665 3665
Size: 7447
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2072 2192 2385 2612 2728 2754 2823
Size: 399 282 120 193 227 116 26 69
Cuts at: 2823 2851 2949 3225 3242 3279 3399 3614 3655
Size: 28 98 276 17 37 120 215 41
Cuts at: 3655 3692 3710 3945 4038 4134 4350 4766 5508
Size: 37 18 235 93 96 216 416 742
Cuts at: 5508 5593 6664 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 416 399 282 276 235
227 216 215 193 120 120 116 98
96 93 85 69 41 37 37 28
26 18 17
BspEI T'CCGG_A
Cuts at: 2650 2941 3531 7110 2650
Size: 291 590 3579 2987
Fragments arranged by size:
3579 2987 590 291
BspHI T'CATG_A
Cuts at: 5336 7066 5336
Size: 1730 5717
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2181 2487 3353 4378 4802 844
Size: 1337 306 866 1025 424 3489
Fragments arranged by size:
3489 1337 1025 866 424 306
BsrBI CCG'CTC
Cuts at: 1394 1823 3898 4375 5334 1394
Size: 429 2075 477 959 3507
Fragments arranged by size:
3507 2075 959 477 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5942 6116 65
Size: 1183 4694 174 1396
Fragments arranged by size:
4694 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2863 4139 6091 6278 1700
Size: 110 1053 1276 1952 187 2869
Fragments arranged by size:
2869 1952 1276 1053 187 110
BsrGI T'GTAC_A
Cuts at: 96 4443 96
Size: 4347 3100
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7447
BssSI C'ACGA_G
Cuts at: 5507 6519 5507
Size: 1012 6435
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2389 794
Size: 1595 5852
BstBI TT'CG_AA
Cuts at: 6251 6251
Size: 7447
BstEII G'GTnAC_C
Cuts at: 1184 4459 1184
Size: 3275 4172
BstXI CCAn_nnnn'nTGG
Cuts at: 3391 4309 3391
Size: 918 6529
BstYI r'GATC_y
Cuts at: 1972 3028 3166 3426 3534 3555 4581 4777 5144
Size: 1056 138 260 108 21 1026 196 367
Cuts at: 5144 5529 5546 6998 7084 7096 7442 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1056 1026 385 367 346 260
196 138 108 86 21 17 12
Bsu36I CC'TnA_GG
Cuts at: 2778 3971 2778
Size: 1193 6254
BtgI C'CryG_G
Cuts at: 513 1065 2004 2266 3891 4308 4500 513
Size: 552 939 262 1625 417 192 3460
Fragments arranged by size:
3460 1625 939 552 417 262 192
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3838 4096 487
Size: 683 56 2612 258 3838
Fragments arranged by size:
3838 2612 683 258 56
BtsI GCAGTG_nn'
Cuts at: 3229 3738 4963 5757 5777 3229
Size: 509 1225 794 20 4899
Fragments arranged by size:
4899 1225 794 509 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7447
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7412
DraI TTT'AAA
Cuts at: 4751 4751
Size: 7447
DraIII CAC_nnn'GTG
Cuts at: 2389 2389
Size: 7447
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6454 809
Size: 507 5138 1802
Fragments arranged by size:
5138 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2031 3324 3411
Size: 54 1425 50 275 18 201 1293 87
Cuts at: 3411 3662 4137 4201 4894 5783 7412 8
Size: 251 475 64 693 889 1629 43
Fragments arranged by size:
1629 1425 1293 889 693 475 275 251
201 87 64 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2031 3662 4137 4894 1537
Size: 275 219 1631 475 757 4090
Fragments arranged by size:
4090 1631 757 475 275 219
EarI CTCTTCn'nnn_
Cuts at: 1353 3779 5377 1353
Size: 2426 1598 3423
Fragments arranged by size:
3423 2426 1598
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2092 6034 6408 6554 387
Size: 1064 138 503 3942 374 146 1280
Fragments arranged by size:
3942 1280 1064 503 374 146 138
EcoICRI GAG'CTC
Cuts at: 2190 2821 2947 4036 2190
Size: 631 126 1089 5601
Fragments arranged by size:
5601 1089 631 126
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2129 2387 2735 2768 2799 2853 2889
Size: 393 497 258 348 33 31 54 36
Cuts at: 2889 2913 2933 2943 2973 3003 3051 3077 3147
Size: 24 20 10 30 30 48 26 70
Cuts at: 3147 3515 3653 3959 4027 4255 4342 4538 4598
Size: 368 138 306 68 228 87 196 60
Cuts at: 4598 4664 5510 6107 6894 7162 1239
Size: 66 846 597 787 268 1524
Fragments arranged by size:
1524 846 787 597 497 393 368 348
306 268 258 228 196 138 87 70
68 66 60 54 48 36 33 31
30 30 26 24 20 10
EcoNI CCTnn'n_nnAGG
Cuts at: 2249 4520 2249
Size: 2271 5176
EcoO109I rG'GnC_Cy
Cuts at: 2470 3876 4021 2470
Size: 1406 145 5896
Fragments arranged by size:
5896 1406 145
EcoRI G'AATT_C
Cuts at: 4756 5134 4756
Size: 378 7069
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4672 4759 4672
Size: 87 7360
FspI TGC'GCA
Cuts at: 2249 5953 2249
Size: 3704 3743
HaeII r_GCGC'y
Cuts at: 1678 2052 2290 2722 2996 3074 3287 4006 4336
Size: 374 238 432 274 78 213 719 330
Cuts at: 4336 6594 1678
Size: 2258 2531
Fragments arranged by size:
2531 2258 719 432 374 330 274 238
213 78
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2294 2326 2612 2644 2771
Size: 32 135 32 722 32 286 32 127
Cuts at: 2771 2803 3932 3964 4659 4691 6088 6120 6162
Size: 32 1129 32 695 32 1397 32 42
Cuts at: 6162 6194 1373
Size: 32 2626
Fragments arranged by size:
2626 1397 1129 722 695 286 135 127
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2645 4791 5037 669
Size: 988 281 707 2146 246 3079
Fragments arranged by size:
3079 2146 988 707 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7447
HpaI GTT'AAC
Cuts at: 5037 5037
Size: 7447
KasI G'GCGC_C
Cuts at: 2048 3283 2048
Size: 1235 6212
KpnI G_GTAC'C
Cuts at: 4772 4772
Size: 7447
MfeI C'AATT_G
Cuts at: 5046 5046
Size: 7447
MluI A'CGCG_T
Cuts at: 7323 7323
Size: 7447
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2577 2918 4179 6561 6745 1305
Size: 1272 341 1261 2382 184 2007
Fragments arranged by size:
2382 2007 1272 1261 341 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3326 3413 4203 10
Size: 54 1425 343 1494 87 790 3254
Fragments arranged by size:
3254 1494 1425 790 343 87 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3743 4307 4457 5405 5764 5923 518
Size: 648 2577 564 150 948 359 159 2042
Fragments arranged by size:
2577 2042 948 648 564 359 159 150
MspA1I CmG'CkG
Cuts at: 1606 2059 2130 2260 2674 2746 2969 3256 4104
Size: 453 71 130 414 72 223 287 848
Cuts at: 4104 4863 5540 6688 6933 1606
Size: 759 677 1148 245 2120
Fragments arranged by size:
2120 1148 848 759 677 453 414 287
245 223 130 72 71
NaeI GCC'GGC
Cuts at: 2865 4141 2865
Size: 1276 6171
NarI GG'CG_CC
Cuts at: 2049 3284 2049
Size: 1235 6212
NcoI C'CATG_G
Cuts at: 513 1065 2004 4500 513
Size: 552 939 2496 3460
Fragments arranged by size:
3460 2496 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7447
NgoMIV G'CCGG_C
Cuts at: 2863 4139 2863
Size: 1276 6171
NheI G'CTAG_C
Cuts at: 1052 2827 1052
Size: 1775 5672
NotI GC'GGCC_GC
Cuts at: 4894 4894
Size: 7447
NspI r_CATG'y
Cuts at: 1132 1229 2020 2701 4444 4805 6350 1132
Size: 97 791 681 1743 361 1545 2229
Fragments arranged by size:
2229 1743 1545 791 681 361 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7447
PciI A'CATG_T
Cuts at: 4440 6346 4440
Size: 1906 5541
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3235 4390 1108
Size: 2127 1155 4165
Fragments arranged by size:
4165 2127 1155
PfoI T'CCnGG_A
Cuts at: 2472 4185 2472
Size: 1713 5734
PmeI GTTT'AAAC
Cuts at: 4751 4751
Size: 7447
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3563 3595 5516 5548 7029 7061 3563
Size: 32 1921 32 1481 32 3949
Fragments arranged by size:
3949 1921 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2470 2470
Size: 7447
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7447
PsiI TTA'TAA
Cuts at: 5017 5017
Size: 7447
PspXI vC'TCGA_Gb
Cuts at: 2822 2844 2822
Size: 22 7425
PstI C_TGCA'G
Cuts at: 830 3474 3831 4554 4799 830
Size: 2644 357 723 245 3478
Fragments arranged by size:
3478 2644 723 357 245
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7447
PvuII CAG'CTG
Cuts at: 2130 2260 2674 2746 2969 2130
Size: 130 414 72 223 6608
Fragments arranged by size:
6608 414 223 130 72
RsrII CG'GwC_CG
Cuts at: 2346 2346
Size: 7447
SacI G_AGCT'C
Cuts at: 2192 2823 2949 4038 2192
Size: 631 126 1089 5601
Fragments arranged by size:
5601 1089 631 126
SalI G'TCGA_C
Cuts at: 1936 4789 1936
Size: 2853 4594
SbfI CC_TGCA'GG
Cuts at: 4799 4799
Size: 7447
ScaI AGT'ACT
Cuts at: 2627 5695 7407 2627
Size: 3068 1712 2667
Fragments arranged by size:
3068 2667 1712
SexAI A'CCwGG_T
Cuts at: 2385 3723 2385
Size: 1338 6109
SfcI C'TryA_G
Cuts at: 826 1045 2443 3470 3827 3847 4550 4795 5930
Size: 219 1398 1027 357 20 703 245 1135
Cuts at: 5930 6611 6802 826
Size: 681 191 1471
Fragments arranged by size:
1471 1398 1135 1027 703 681 357 245
219 191 20
SfiI GGCCn_nnn'nGGCC
Cuts at: 2030 2030
Size: 7447
SfoI GGC'GCC
Cuts at: 2050 3285 2050
Size: 1235 6212
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7447
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7447
SmaI CCC'GGG
Cuts at: 1872 4774 1872
Size: 2902 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2666 2822 2844 3431 4724 5551
Size: 197 926 723 156 22 587 1293 827
Cuts at: 5551 6452 6714 820
Size: 901 262 1553
Fragments arranged by size:
1553 1293 926 901 827 723 587 262
197 156 22
SnaBI TAC'GTA
Cuts at: 493 5238 493
Size: 4745 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7447
SphI G_CATG'C
Cuts at: 1132 4805 1132
Size: 3673 3774
SspI AAT'ATT
Cuts at: 5 52 5371 5
Size: 47 5319 2081
Fragments arranged by size:
5319 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7447
StyI C'CwwG_G
Cuts at: 513 1065 2004 4500 4662 4734 4886 513
Size: 552 939 2496 162 72 152 3074
Fragments arranged by size:
3074 2496 939 552 162 152 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2477 2866 3323 4340 5474 5491 5644
Size: 1255 897 389 457 1017 1134 17 153
Cuts at: 5644 5829 6274 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1134 1017 897 457 445 389
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2625 4443 5693
Size: 274 80 33 51 1443 648 1818 1250
Cuts at: 5693 7405 96
Size: 1712 138
Fragments arranged by size:
1818 1712 1443 1250 648 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4049 4081 7407 7439 4049
Size: 32 3326 32 4057
Fragments arranged by size:
4057 3326 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4637 1316
Size: 3321 4126
XbaI T'CTAG_A
Cuts at: 2525 3049 4783 2525
Size: 524 1734 5189
Fragments arranged by size:
5189 1734 524
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4082 4306 4082
Size: 224 7223
XhoI C'TCGA_G
Cuts at: 1943 2822 2844 1943
Size: 879 22 6546
Fragments arranged by size:
6546 879 22
XmaI C'CCGG_G
Cuts at: 1870 4772 1870
Size: 2902 4545
XmnI GAAnn'nnTTC
Cuts at: 3797 5576 3797
Size: 1779 5668
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5255 276
Size: 53 83 186 936 3721 2468
Fragments arranged by size:
3721 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI
PstI PvuI PvuII RsrII SacI SalI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
ApaI AscI AvrII BfrBI BsiWI BstZ17I EcoRV FseI
FspAI NruI NsiI PacI PmlI PspOMI PsrI SacII
SanDI SapI SrfI SwaI