Restriction map for
pFN21AE2119
(Circular) (Six-base) MAPSORT of: pFN21AE2119.seq Check: 3228 from: 1 to: 7441
pFN21AE2119 7441 bp
With 182 enzymes: SgfI *
March 28, 2012 14:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5251 278
Size: 53 83 186 936 3715 2468
Fragments arranged by size:
3715 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2259 4784 1937
Size: 322 2525 4594
Fragments arranged by size:
4594 2525 322
Acc65I G'GTAC_C
Cuts at: 4238 4762 4238
Size: 524 6917
AclI AA'CG_TT
Cuts at: 1563 2622 2935 5568 5941 1563
Size: 1059 313 2633 373 3063
Fragments arranged by size:
3063 2633 1059 373 313
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2330 2897 3426 4716 5504 6888 7156 1632
Size: 698 567 529 1290 788 1384 268 1917
Fragments arranged by size:
1917 1384 1290 788 698 567 529 268
AfeI AGC'GCT
Cuts at: 4484 4577 4484
Size: 93 7348
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7244
AflIII A'CryG_T
Cuts at: 1203 4299 6340 7317 1203
Size: 3096 2041 977 1327
Fragments arranged by size:
3096 2041 1327 977
AgeI A'CCGG_T
Cuts at: 6272 6272
Size: 7441
AhdI GACnn_n'nnGTC
Cuts at: 4303 6170 4303
Size: 1867 5574
AleI CACnn'nnGTG
Cuts at: 1166 3924 1166
Size: 2758 4683
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7441
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3178 3210 3178
Size: 32 7409
AlwNI CAG_nnn'CTG
Cuts at: 3014 4636 5184 6756 3014
Size: 1622 548 1572 3699
Fragments arranged by size:
3699 1622 1572 548
ApaI G_GGCC'C
Cuts at: 2103 2109 2103
Size: 6 7435
ApaLI G'TGCA_C
Cuts at: 2940 5498 6654 2940
Size: 2558 1156 3727
Fragments arranged by size:
3727 2558 1156
ApoI r'AATT_y
Cuts at: 1493 2746 3399 3414 3934 4380 4750 4976 5128
Size: 1253 653 15 520 446 370 226 152
Cuts at: 5128 7237 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1253 653 520 446 370 226
152 15
AseI AT'TA_AT
Cuts at: 160 1032 2982 5995 160
Size: 872 1950 3013 1606
Fragments arranged by size:
3013 1950 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7441
AvaI C'yCGr_G
Cuts at: 1870 1943 2102 4766 1870
Size: 73 159 2664 4545
Fragments arranged by size:
4545 2664 159 73
BamHI G'GATC_C
Cuts at: 4771 5138 4771
Size: 367 7074
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4089 4238 4762 6217 618
Size: 325 217 627 2302 149 524 1455 1842
Fragments arranged by size:
2302 1842 1455 627 524 325 217 149
BanII G_rGCy'C
Cuts at: 1391 2103 2109 2473 2694 3072 3647 4760 1391
Size: 712 6 364 221 378 575 1113 4072
Fragments arranged by size:
4072 1113 712 575 378 364 221 6
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2430 4371 928
Size: 971 531 1941 3998
Fragments arranged by size:
3998 1941 971 531
BbvCI CC'TCA_GC
Cuts at: 4150 4150
Size: 7441
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5632 5666 1903
Size: 34 3695 34 3678
Fragments arranged by size:
3695 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4413 5333 6543 4413
Size: 920 1210 5311
Fragments arranged by size:
5311 1210 920
BclI T'GATC_A
Cuts at: 1548 2485 4656 1548
Size: 937 2171 4333
Fragments arranged by size:
4333 2171 937
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2072 4338 6052
Size: 107 122 71 631 605 400 2266 1714
Cuts at: 6052 136
Size: 1525
Fragments arranged by size:
2266 1714 1525 631 605 400 122 107
71
BglII A'GATC_T
Cuts at: 7436 7436
Size: 7441
BlpI GC'TnA_GC
Cuts at: 2996 4858 2996
Size: 1862 5579
Bme1580I G_kGCm'C
Cuts at: 1790 2103 2109 2683 2944 5502 6658 1790
Size: 313 6 574 261 2558 1156 2573
Fragments arranged by size:
2573 2558 1156 574 313 261 6
BmgBI CAC'GTC
Cuts at: 1318 2233 1318
Size: 915 6526
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2028 2643 3779
Size: 416 53 83 399 24 611 615 1136
Cuts at: 3779 5172 6130 442
Size: 1393 958 1753
Fragments arranged by size:
1753 1393 1136 958 615 611 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 4148 4674 1056
Size: 3092 526 3823
Fragments arranged by size:
3823 3092 526
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2031 2031
Size: 7441
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2457 6101 1239
Size: 1218 3644 2579
Fragments arranged by size:
3644 2579 1218
Bpu10I CC'TnA_GC
Cuts at: 2174 4150 4334 2174
Size: 1976 184 5281
Fragments arranged by size:
5281 1976 184
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4525 5566 6431 6729 4525
Size: 1041 865 298 5237
Fragments arranged by size:
5237 1041 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6104 882
Size: 617 4605 2219
Fragments arranged by size:
4605 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5232 493
Size: 711 4028 2702
Fragments arranged by size:
4028 2702 711
BsaBI GATnn'nnATC
Cuts at: 3608 7435 3608
Size: 3827 3614
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3177 3956 5248 5630
Size: 53 83 186 936 1644 779 1292 382
Cuts at: 5630 6265 275
Size: 635 1451
Fragments arranged by size:
1644 1451 1292 936 779 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2017 4221 5874 6272 6546 6693 7104 2017
Size: 2204 1653 398 274 147 411 2354
Fragments arranged by size:
2354 2204 1653 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3507 3537 3932 3962 1178
Size: 30 165 30 2104 30 395 30 4657
Fragments arranged by size:
4657 2104 395 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3637 1186
Size: 2451 4990
BseYI C'CCAG_C
Cuts at: 6644 7140 6644
Size: 496 6945
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3254 3600 1163
Size: 573 1518 346 5004
Fragments arranged by size:
5004 1518 573 346
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2030 2076 2142 4891 5652 6680
Size: 275 186 29 46 66 2749 761 1028
Cuts at: 6680 1540
Size: 2301
Fragments arranged by size:
2749 2301 1028 761 275 186 66 46
29
BsiHKAI G_wGCw'C
Cuts at: 2473 2944 4047 5502 5587 6658 2473
Size: 471 1103 1455 85 1071 3256
Fragments arranged by size:
3256 1455 1103 1071 471 85
BsmI GAATG_Cn'
Cuts at: 4393 4565 4952 5045 4393
Size: 172 387 93 6789
Fragments arranged by size:
6789 387 172 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3947 3947
Size: 7441
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2103 2109 2473 2683 2694 2944 3072
Size: 399 313 6 364 210 11 250 128
Cuts at: 3072 3647 4047 4760 5502 5587 6658 1391
Size: 575 400 713 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 713 575 400 399 364
313 250 210 128 85 11 6
BspEI T'CCGG_A
Cuts at: 4221 7104 4221
Size: 2883 4558
BspHI T'CATG_A
Cuts at: 3222 3273 4432 5330 7060 3222
Size: 51 1159 898 1730 3603
Fragments arranged by size:
3603 1730 1159 898 51
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3375 4023 4796 844
Size: 2531 648 773 3489
Fragments arranged by size:
3489 2531 773 648
BsrBI CCG'CTC
Cuts at: 1394 1823 5328 1394
Size: 429 3505 3507
Fragments arranged by size:
3507 3505 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3099 3123 5936 6110 65
Size: 1183 1851 24 2813 174 1396
Fragments arranged by size:
2813 1851 1396 1183 174 24
BsrFI r'CCGG_y
Cuts at: 1700 1810 2185 6085 6272 1700
Size: 110 375 3900 187 2869
Fragments arranged by size:
3900 2869 375 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7441
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7441
BssSI C'ACGA_G
Cuts at: 3170 4076 5501 6513 3170
Size: 906 1425 1012 4098
Fragments arranged by size:
4098 1425 1012 906
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2899 2913 4636 794
Size: 2105 14 1723 3599
Fragments arranged by size:
3599 2105 1723 14
BstBI TT'CG_AA
Cuts at: 3480 6245 3480
Size: 2765 4676
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7441
BstXI CCAn_nnnn'nTGG
Cuts at: 4416 4416
Size: 7441
BstYI r'GATC_y
Cuts at: 1972 2304 3154 4771 5138 5523 5540 6992 7078
Size: 332 850 1617 367 385 17 1452 86
Cuts at: 7078 7090 7436 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1617 1452 850 385 367 346 332
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4167 4260 4268 4604 4167
Size: 93 8 336 7004
Fragments arranged by size:
7004 336 93 8
BtgI C'CryG_G
Cuts at: 513 1065 2049 3651 3991 513
Size: 552 984 1602 340 3963
Fragments arranged by size:
3963 1602 984 552 340
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6702
Fragments arranged by size:
6702 683 56
BtsI GCAGTG_nn'
Cuts at: 2909 4957 5751 5771 2909
Size: 2048 794 20 4579
Fragments arranged by size:
4579 2048 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7441
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7406
DraI TTT'AAA
Cuts at: 2575 4102 4745 2575
Size: 1527 643 5271
Fragments arranged by size:
5271 1527 643
DraIII CAC_nnn'GTG
Cuts at: 2853 2853
Size: 7441
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6448 809
Size: 507 5132 1802
Fragments arranged by size:
5132 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2027 2073 2361
Size: 54 1425 50 275 18 197 46 288
Cuts at: 2361 3588 4888 5777 7406 8
Size: 1227 1300 889 1629 43
Fragments arranged by size:
1629 1425 1300 1227 889 288 275 197
54 50 46 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2027 2073 4888 1537
Size: 275 215 46 2815 4090
Fragments arranged by size:
4090 2815 275 215 46
EarI CTCTTCn'nnn_
Cuts at: 1353 2732 3248 3817 5371 1353
Size: 1379 516 569 1554 3423
Fragments arranged by size:
3423 1554 1379 569 516
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2401 2620 6028 6402 6548 387
Size: 1064 138 812 219 3408 374 146 1280
Fragments arranged by size:
3408 1280 1064 812 374 219 146 138
EcoICRI GAG'CTC
Cuts at: 2471 2471
Size: 7441
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2330 2457 2897 3426 4716 5504 6101
Size: 393 698 127 440 529 1290 788 597
Cuts at: 6101 6888 7156 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1290 788 787 698 597 529 440
393 268 127
EcoO109I rG'GnC_Cy
Cuts at: 4264 4264
Size: 7441
EcoRI G'AATT_C
Cuts at: 4750 5128 4750
Size: 378 7063
EcoRV GAT'ATC
Cuts at: 2371 3682 2371
Size: 1311 6130
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2402 2402
Size: 7441
FspI TGC'GCA
Cuts at: 5947 5947
Size: 7441
HaeII r_GCGC'y
Cuts at: 1678 2013 4486 4579 6588 1678
Size: 335 2473 93 2009 2531
Fragments arranged by size:
2531 2473 2009 335 93
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2315 2347 2726 2758 3139
Size: 32 135 32 743 32 379 32 381
Cuts at: 3139 3171 3276 3308 4289 4321 6082 6114 6156
Size: 32 105 32 981 32 1761 32 42
Cuts at: 6156 6188 1373
Size: 32 2626
Fragments arranged by size:
2626 1761 981 743 381 379 135 105
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2260 4785 5031 669
Size: 988 281 322 2525 246 3079
Fragments arranged by size:
3079 2525 988 322 281 246
HindIII A'AGCT_T
Cuts at: 748 2870 748
Size: 2122 5319
HpaI GTT'AAC
Cuts at: 5031 5031
Size: 7441
KpnI G_GTAC'C
Cuts at: 4242 4766 4242
Size: 524 6917
MfeI C'AATT_G
Cuts at: 2587 5040 2587
Size: 2453 4988
MluI A'CGCG_T
Cuts at: 7317 7317
Size: 7441
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3887 6555 6739 1305
Size: 2582 2668 184 2007
Fragments arranged by size:
2668 2582 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3590 10
Size: 54 1425 343 1758 3861
Fragments arranged by size:
3861 1758 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3272 3924 4593 5399 5758 5917 518
Size: 648 2106 652 669 806 359 159 2042
Fragments arranged by size:
2106 2042 806 669 652 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2041 2063 2072 2350 2595 4154 4857 5534
Size: 435 22 9 278 245 1559 703 677
Cuts at: 5534 6682 6927 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1559 1148 703 677 435 278 245
245 22 9
NcoI C'CATG_G
Cuts at: 513 1065 3991 513
Size: 552 2926 3963
Fragments arranged by size:
3963 2926 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7441
NheI G'CTAG_C
Cuts at: 1052 4144 4670 1052
Size: 3092 526 3823
Fragments arranged by size:
3823 3092 526
NotI GC'GGCC_GC
Cuts at: 2027 2073 4888 2027
Size: 46 2815 4580
Fragments arranged by size:
4580 2815 46
NspI r_CATG'y
Cuts at: 1132 1229 3620 4003 4303 4799 6344 1132
Size: 97 2391 383 300 496 1545 2229
Fragments arranged by size:
2391 2229 1545 496 383 300 97
PasI CC'CwG_GG
Cuts at: 1348 2090 3002 1348
Size: 742 912 5787
Fragments arranged by size:
5787 912 742
PciI A'CATG_T
Cuts at: 4299 6340 4299
Size: 2041 5400
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3597 1108
Size: 2489 4952
PfoI T'CCnGG_A
Cuts at: 2373 2373
Size: 7441
PmeI GTTT'AAAC
Cuts at: 4745 4745
Size: 7441
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5510 5542 7023 7055 5510
Size: 32 1481 32 5896
Fragments arranged by size:
5896 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4264 4264
Size: 7441
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7441
PsiI TTA'TAA
Cuts at: 5011 5011
Size: 7441
PspOMI G'GGCC_C
Cuts at: 2099 2105 2099
Size: 6 7435
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4265 4297 4265
Size: 32 7409
PstI C_TGCA'G
Cuts at: 830 2166 2202 4641 4793 830
Size: 1336 36 2439 152 3478
Fragments arranged by size:
3478 2439 1336 152 36
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7441
PvuII CAG'CTG
Cuts at: 2063 2350 4154 2063
Size: 287 1804 5350
Fragments arranged by size:
5350 1804 287
SacI G_AGCT'C
Cuts at: 2473 2473
Size: 7441
SalI G'TCGA_C
Cuts at: 1936 2258 4783 1936
Size: 322 2525 4594
Fragments arranged by size:
4594 2525 322
SbfI CC_TGCA'GG
Cuts at: 4793 4793
Size: 7441
ScaI AGT'ACT
Cuts at: 2861 3768 5689 7401 2861
Size: 907 1921 1712 2901
Fragments arranged by size:
2901 1921 1712 907
SexAI A'CCwGG_T
Cuts at: 4535 4535
Size: 7441
SfcI C'TryA_G
Cuts at: 826 1045 2162 2198 2218 3905 4486 4520 4637
Size: 219 1117 36 20 1687 581 34 117
Cuts at: 4637 4789 5924 6605 6796 826
Size: 152 1135 681 191 1471
Fragments arranged by size:
1687 1471 1135 1117 681 581 219 191
152 117 36 34 20
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7441
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7441
SmaI CCC'GGG
Cuts at: 1872 2104 4768 1872
Size: 232 2664 4545
Fragments arranged by size:
4545 2664 232
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4504 5545 6446 6708 820
Size: 197 926 2561 1041 901 262 1553
Fragments arranged by size:
2561 1553 1041 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5232 493
Size: 4739 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7441
SphI G_CATG'C
Cuts at: 1132 3620 4799 1132
Size: 2488 1179 3774
Fragments arranged by size:
3774 2488 1179
SrfI GCCC'GGGC
Cuts at: 2104 2104
Size: 7441
SspI AAT'ATT
Cuts at: 5 52 5365 5
Size: 47 5313 2081
Fragments arranged by size:
5313 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7441
StyI C'CwwG_G
Cuts at: 513 1065 2965 3639 3991 4514 4880 513
Size: 552 1900 674 352 523 366 3074
Fragments arranged by size:
3074 1900 674 552 523 366 352
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4601 5468 5485 5638 5823 6268 325
Size: 1255 3021 867 17 153 185 445 1498
Fragments arranged by size:
3021 1498 1255 867 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2859 3766 5687
Size: 274 80 33 51 1443 882 907 1921
Cuts at: 5687 7399 96
Size: 1712 138
Fragments arranged by size:
1921 1712 1443 907 882 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3507 3539 7401 7433 3507
Size: 32 3862 32 3515
Fragments arranged by size:
3862 3515 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7441
XbaI T'CTAG_A
Cuts at: 4294 4777 4294
Size: 483 6958
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3137 4413 3137
Size: 1276 6165
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7441
XmaI C'CCGG_G
Cuts at: 1870 2102 4766 1870
Size: 232 2664 4545
Fragments arranged by size:
4545 2664 232
XmnI GAAnn'nnTTC
Cuts at: 2427 4217 5570 2427
Size: 1790 1353 4298
Fragments arranged by size:
4298 1790 1353
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5249 276
Size: 53 83 186 936 3715 2468
Fragments arranged by size:
3715 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I
EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspXI RsrII SacII SanDI SapI
SfiI SfoI SwaI