Restriction map for
pFN21AE2118
(Circular) (Six-base) MAPSORT of: pFN21AE2118.seq Check: 4319 from: 1 to: 7282
pFN21AE2118 7282 bp
With 182 enzymes: SgfI *
March 28, 2012 12:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2697 2697
Size: 7282
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5092 278
Size: 53 83 186 936 3556 2468
Fragments arranged by size:
3556 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2060 2877 4182 4625 1937
Size: 123 817 1305 443 4594
Fragments arranged by size:
4594 1305 817 443 123
Acc65I G'GTAC_C
Cuts at: 4603 4603
Size: 7282
AclI AA'CG_TT
Cuts at: 1563 5409 5782 1563
Size: 3846 373 3063
Fragments arranged by size:
3846 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2528 3440 4240 4344 4401 5345 6729 6997
Size: 896 912 800 104 57 944 1384 268
Cuts at: 6997 1632
Size: 1917
Fragments arranged by size:
1917 1384 944 912 896 800 268 104
57
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7085
AflIII A'CryG_T
Cuts at: 1203 4374 6181 7158 1203
Size: 3171 1807 977 1327
Fragments arranged by size:
3171 1807 1327 977
AgeI A'CCGG_T
Cuts at: 6113 6113
Size: 7282
AhdI GACnn_n'nnGTC
Cuts at: 2884 3844 6011 2884
Size: 960 2167 4155
Fragments arranged by size:
4155 2167 960
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7282
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7282
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2020 2052 2020
Size: 32 7250
AlwNI CAG_nnn'CTG
Cuts at: 3085 3499 3953 4228 5025 6597 3085
Size: 414 454 275 797 1572 3770
Fragments arranged by size:
3770 1572 797 454 414 275
ApaI G_GGCC'C
Cuts at: 2177 2410 3605 4490 2177
Size: 233 1195 885 4969
Fragments arranged by size:
4969 1195 885 233
ApaLI G'TGCA_C
Cuts at: 2542 5339 6495 2542
Size: 2797 1156 3329
Fragments arranged by size:
3329 2797 1156
ApoI r'AATT_y
Cuts at: 1493 4591 4817 4969 7078 1493
Size: 3098 226 152 2109 1697
Fragments arranged by size:
3098 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5836 160
Size: 872 4804 1606
Fragments arranged by size:
4804 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7282
AvaI C'yCGr_G
Cuts at: 1870 1943 2117 3435 3864 3918 4607 1870
Size: 73 174 1318 429 54 689 4545
Fragments arranged by size:
4545 1318 689 429 174 73 54
AvrII C'CTAG_G
Cuts at: 3742 3742
Size: 7282
BamHI G'GATC_C
Cuts at: 3895 4612 4979 3895
Size: 717 367 6198
Fragments arranged by size:
6198 717 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3405 4603 6058 618
Size: 325 217 627 1618 1198 1455 1842
Fragments arranged by size:
1842 1618 1455 1198 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2177 2402 2410 3261 3605 3947 4391 4490
Size: 786 225 8 851 344 342 444 99
Cuts at: 4490 4601 1391
Size: 111 4072
Fragments arranged by size:
4072 851 786 444 344 342 225 111
99 8
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2355 2413 3816 928
Size: 971 456 58 1403 4394
Fragments arranged by size:
4394 1403 971 456 58
BbvCI CC'TCA_GC
Cuts at: 2950 3106 3757 2950
Size: 156 651 6475
Fragments arranged by size:
6475 651 156
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2226 2260 5473 5507 1903
Size: 34 289 34 3213 34 3678
Fragments arranged by size:
3678 3213 289 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5174 6384 5174
Size: 1210 6072
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7282
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2253 2280 2834
Size: 107 122 71 631 605 581 27 554
Cuts at: 2834 3738 3767 5893 136
Size: 904 29 2126 1525
Fragments arranged by size:
2126 1525 904 631 605 581 554 122
107 71 29 27
BglII A'GATC_T
Cuts at: 2757 7277 2757
Size: 4520 2762
BlpI GC'TnA_GC
Cuts at: 3946 4699 3946
Size: 753 6529
Bme1580I G_kGCm'C
Cuts at: 1790 2177 2410 2546 3000 3410 3605 4490 5343
Size: 387 233 136 454 410 195 885 853
Cuts at: 5343 6499 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 885 853 454 410 387 233
195 136
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7282
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4305 5013 5971
Size: 416 53 83 399 24 2888 708 958
Cuts at: 5971 442
Size: 1753
Fragments arranged by size:
2888 1753 958 708 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7282
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4236 4236
Size: 7282
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2986 3323 3738 3990 4563 5942 1239
Size: 1747 337 415 252 573 1379 2579
Fragments arranged by size:
2579 1747 1379 573 415 337 252
Bpu10I CC'TnA_GC
Cuts at: 2401 2950 3106 3377 3757 2401
Size: 549 156 271 380 5926
Fragments arranged by size:
5926 549 380 271 156
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2288 2699 3344 3515 5407 6272 6570 2288
Size: 411 645 171 1892 865 298 3000
Fragments arranged by size:
3000 1892 865 645 411 298 171
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2015 2463 5945 882
Size: 617 516 448 3482 2219
Fragments arranged by size:
3482 2219 617 516 448
BsaAI yAC'GTr
Cuts at: 493 1204 5073 493
Size: 711 3869 2702
Fragments arranged by size:
3869 2702 711
BsaBI GATnn'nnATC
Cuts at: 7276 7276
Size: 7282
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2132 5089 5471 6106
Size: 53 83 186 936 599 2957 382 635
Cuts at: 6106 275
Size: 1451
Fragments arranged by size:
2957 1451 936 635 599 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5715 6113 6387 6534 6945 5715
Size: 398 274 147 411 6052
Fragments arranged by size:
6052 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2020 2050 2536 2566 3613
Size: 30 165 30 617 30 486 30 1047
Cuts at: 3613 3643 4364 4394 1178
Size: 30 721 30 4066
Fragments arranged by size:
4066 1047 721 617 486 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2459 3259 3262 3269 3993 4181 1186
Size: 1273 800 3 7 724 188 4287
Fragments arranged by size:
4287 1273 800 724 188 7 3
BseYI C'CCAG_C
Cuts at: 2189 3890 4082 4089 4102 4442 4564 6485 6981
Size: 1701 192 7 13 340 122 1921 496
Cuts at: 6981 2189
Size: 2490
Fragments arranged by size:
2490 1921 1701 496 340 192 122 13
7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2095 2669 2675 3164 3200 4092 1163
Size: 573 359 574 6 489 36 892 4353
Fragments arranged by size:
4353 892 574 573 489 359 36 6
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4732 5493 6521 1540
Size: 275 186 2731 761 1028 2301
Fragments arranged by size:
2731 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2334 2546 2801 3261 3288 4382 5343 5428 6499
Size: 212 255 460 27 1094 961 85 1071
Cuts at: 6499 2334
Size: 3117
Fragments arranged by size:
3117 1094 1071 961 460 255 212 85
27
BsmI GAATG_Cn'
Cuts at: 4793 4886 4793
Size: 93 7189
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2177 2334 2402 2410 2546 2801 3000
Size: 399 387 157 68 8 136 255 199
Cuts at: 3000 3261 3288 3410 3605 3947 4382 4391 4490
Size: 261 27 122 195 342 435 9 99
Cuts at: 4490 4601 5343 5428 6499 1391
Size: 111 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 435 399 387 342 261
255 199 195 157 136 122 111 99
85 68 27 9 8
BspEI T'CCGG_A
Cuts at: 6945 6945
Size: 7282
BspHI T'CATG_A
Cuts at: 3799 5171 6901 3799
Size: 1372 1730 4180
Fragments arranged by size:
4180 1730 1372
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2394 2691 2697 3687 3849 4637 844
Size: 1550 297 6 990 162 788 3489
Fragments arranged by size:
3489 1550 990 788 297 162 6
BsrBI CCG'CTC
Cuts at: 1394 1823 3400 4351 4363 4483 5169 1394
Size: 429 1577 951 12 120 686 3507
Fragments arranged by size:
3507 1577 951 686 429 120 12
BsrDI GCAATG_nn'
Cuts at: 65 1248 2860 3099 5777 5951 65
Size: 1183 1612 239 2678 174 1396
Fragments arranged by size:
2678 1612 1396 1183 239 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5926 6113 1700
Size: 110 4116 187 2869
Fragments arranged by size:
4116 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4116 96
Size: 4020 3262
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7282
BssSI C'ACGA_G
Cuts at: 5342 6354 5342
Size: 1012 6270
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7282
BstBI TT'CG_AA
Cuts at: 6086 6086
Size: 7282
BstEII G'GTnAC_C
Cuts at: 1184 2193 1184
Size: 1009 6273
BstXI CCAn_nnnn'nTGG
Cuts at: 3716 3716
Size: 7282
BstYI r'GATC_y
Cuts at: 1972 2710 2757 2925 3024 3081 3895 4612 4979
Size: 738 47 168 99 57 814 717 367
Cuts at: 4979 5364 5381 6833 6919 6931 7277 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 814 738 717 385 367 346
168 99 86 57 47 17 12
Bsu36I CC'TnA_GG
Cuts at: 2841 3597 4060 2841
Size: 756 463 6063
Fragments arranged by size:
6063 756 463
BtgI C'CryG_G
Cuts at: 513 1065 2004 2437 2655 3628 3787 513
Size: 552 939 433 218 973 159 4008
Fragments arranged by size:
4008 973 939 552 433 218 159
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2647 487
Size: 683 56 1421 5122
Fragments arranged by size:
5122 1421 683 56
BtsI GCAGTG_nn'
Cuts at: 2249 2263 2396 4109 4798 5592 5612 2249
Size: 14 133 1713 689 794 20 3919
Fragments arranged by size:
3919 1713 794 689 133 20 14
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7282
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7247
DraI TTT'AAA
Cuts at: 4586 4586
Size: 7282
DraIII CAC_nnn'GTG
Cuts at: 3938 3938
Size: 7282
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6289 809
Size: 507 4973 1802
Fragments arranged by size:
4973 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2617 4352 4729
Size: 54 1425 50 275 18 787 1735 377
Cuts at: 4729 5618 7247 8
Size: 889 1629 43
Fragments arranged by size:
1735 1629 1425 889 787 377 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4729 1537
Size: 275 2917 4090
Fragments arranged by size:
4090 2917 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3222 3473 4158 5212 1353
Size: 1869 251 685 1054 3423
Fragments arranged by size:
3423 1869 1054 685 251
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3900 5869 6243 6389 387
Size: 1064 138 2311 1969 374 146 1280
Fragments arranged by size:
2311 1969 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3259 3259
Size: 7282
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2528 2986 3323 3440 3738 3990 4240
Size: 393 896 458 337 117 298 252 250
Cuts at: 4240 4344 4401 4563 5345 5942 6729 6997 1239
Size: 104 57 162 782 597 787 268 1524
Fragments arranged by size:
1524 896 787 782 597 458 393 337
298 268 252 250 162 117 104 57
EcoNI CCTnn'n_nnAGG
Cuts at: 2069 2357 3227 4008 4020 2069
Size: 288 870 781 12 5331
Fragments arranged by size:
5331 870 781 288 12
EcoO109I rG'GnC_Cy
Cuts at: 2125 2173 2272 2362 2406 3207 3439 3601 3602
Size: 48 99 90 44 801 232 162 1
Cuts at: 3602 3676 3739 3752 3861 4013 4056 4086 4486
Size: 74 63 13 109 152 43 30 400
Cuts at: 4486 4487 2125
Size: 1 4920
Fragments arranged by size:
4920 801 400 232 162 152 109 99
90 74 63 48 44 43 30 13
1 1
EcoRI G'AATT_C
Cuts at: 4591 4969 4591
Size: 378 6904
EcoRV GAT'ATC
Cuts at: 3526 3526
Size: 7282
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2827 2827
Size: 7282
FspI TGC'GCA
Cuts at: 2084 5788 2084
Size: 3704 3578
HaeII r_GCGC'y
Cuts at: 1678 2434 3660 6429 1678
Size: 756 1226 2769 2531
Fragments arranged by size:
2769 2531 1226 756
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2702 2734 2870 2902 3089
Size: 32 135 32 1130 32 136 32 187
Cuts at: 3089 3104 3121 3136 3830 3862 3887 3919 4187
Size: 15 17 15 694 32 25 32 268
Cuts at: 4187 4219 4256 4288 4349 4364 4381 4396 5923
Size: 32 37 32 61 15 17 15 1527
Cuts at: 5923 5955 5997 6029 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1527 1130 694 268 187 136 135
61 42 37 32 32 32 32 32
32 32 32 32 32 25 17 17
15 15 15 15
HincII GTy'rAC
Cuts at: 669 1657 1938 2647 2878 4626 4872 669
Size: 988 281 709 231 1748 246 3079
Fragments arranged by size:
3079 1748 988 709 281 246 231
HindIII A'AGCT_T
Cuts at: 748 3390 3467 748
Size: 2642 77 4563
Fragments arranged by size:
4563 2642 77
HpaI GTT'AAC
Cuts at: 4872 4872
Size: 7282
KpnI G_GTAC'C
Cuts at: 4607 4607
Size: 7282
MfeI C'AATT_G
Cuts at: 4881 4881
Size: 7282
MluI A'CGCG_T
Cuts at: 7158 7158
Size: 7282
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3100 4563 6396 6580 1305
Size: 1795 1463 1833 184 2007
Fragments arranged by size:
2007 1833 1795 1463 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5460
Fragments arranged by size:
5460 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2570 2804 5240 5599 5758 518
Size: 648 1404 234 2436 359 159 2042
Fragments arranged by size:
2436 2042 1404 648 359 234 159
MspA1I CmG'CkG
Cuts at: 1606 2189 2596 3110 3352 3698 4106 4698 5375
Size: 583 407 514 242 346 408 592 677
Cuts at: 5375 6523 6768 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 592 583 514 408 407
346 245 242
NcoI C'CATG_G
Cuts at: 513 1065 2004 2655 513
Size: 552 939 651 5140
Fragments arranged by size:
5140 939 651 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7282
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7282
NotI GC'GGCC_GC
Cuts at: 4729 4729
Size: 7282
NspI r_CATG'y
Cuts at: 1132 1229 2731 4237 4378 4640 6185 1132
Size: 97 1502 1506 141 262 1545 2229
Fragments arranged by size:
2229 1545 1506 1502 262 141 97
PasI CC'CwG_GG
Cuts at: 1348 2177 2410 2587 3734 4384 1348
Size: 829 233 177 1147 650 4246
Fragments arranged by size:
4246 1147 829 650 233 177
PciI A'CATG_T
Cuts at: 4374 6181 4374
Size: 1807 5475
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2961 4277 1108
Size: 1853 1316 4113
Fragments arranged by size:
4113 1853 1316
PfoI T'CCnGG_A
Cuts at: 2379 2379
Size: 7282
PmeI GTTT'AAAC
Cuts at: 4586 4586
Size: 7282
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2020 2052 5351 5383 6864 6896 2020
Size: 32 3299 32 1481 32 2406
Fragments arranged by size:
3299 2406 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2125 2362 3207 3676 3752 3861 4056 4086 2125
Size: 237 845 469 76 109 195 30 5321
Fragments arranged by size:
5321 845 469 237 195 109 76 30
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7282
PsiI TTA'TAA
Cuts at: 4852 4852
Size: 7282
PspOMI G'GGCC_C
Cuts at: 2173 2406 3601 4486 2173
Size: 233 1195 885 4969
Fragments arranged by size:
4969 1195 885 233
PstI C_TGCA'G
Cuts at: 830 2151 2244 2391 4634 830
Size: 1321 93 147 2243 3478
Fragments arranged by size:
3478 2243 1321 147 93
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7282
PvuII CAG'CTG
Cuts at: 2189 2596 3110 3352 3698 4106 2189
Size: 407 514 242 346 408 5365
Fragments arranged by size:
5365 514 408 407 346 242
SacI G_AGCT'C
Cuts at: 3261 3261
Size: 7282
SalI G'TCGA_C
Cuts at: 1936 2876 4624 1936
Size: 940 1748 4594
Fragments arranged by size:
4594 1748 940
SanDI GG'GwC_CC
Cuts at: 3752 3861 4086 3752
Size: 109 225 6948
Fragments arranged by size:
6948 225 109
SbfI CC_TGCA'GG
Cuts at: 4634 4634
Size: 7282
ScaI AGT'ACT
Cuts at: 5530 7242 5530
Size: 1712 5570
SfcI C'TryA_G
Cuts at: 826 1045 2147 2240 2387 4630 5765 6446 6637
Size: 219 1102 93 147 2243 1135 681 191
Cuts at: 6637 826
Size: 1471
Fragments arranged by size:
2243 1471 1135 1102 681 219 191 147
93
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7282
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7282
SmaI CCC'GGG
Cuts at: 1872 3437 3866 3920 4609 1872
Size: 1565 429 54 689 4545
Fragments arranged by size:
4545 1565 689 429 54
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2267 2714 3359 3530 5386 6287
Size: 197 926 324 447 645 171 1856 901
Cuts at: 6287 6549 820
Size: 262 1553
Fragments arranged by size:
1856 1553 926 901 645 447 324 262
197 171
SnaBI TAC'GTA
Cuts at: 493 5073 493
Size: 4580 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7282
SphI G_CATG'C
Cuts at: 1132 4640 1132
Size: 3508 3774
SspI AAT'ATT
Cuts at: 5 52 5206 5
Size: 47 5154 2081
Fragments arranged by size:
5154 2081 47
StuI AGG'CCT
Cuts at: 1517 3414 3770 1517
Size: 1897 356 5029
Fragments arranged by size:
5029 1897 356
StyI C'CwwG_G
Cuts at: 513 1065 2004 2655 2778 3660 3742 4721 513
Size: 552 939 651 123 882 82 979 3074
Fragments arranged by size:
3074 979 939 882 651 552 123 82
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2383 2561 2737 3579 5309 5326 5479
Size: 1255 803 178 176 842 1730 17 153
Cuts at: 5479 5664 6109 325
Size: 185 445 1498
Fragments arranged by size:
1730 1498 1255 842 803 445 185 178
176 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3115 4116 5528
Size: 274 80 33 51 1443 1138 1001 1412
Cuts at: 5528 7240 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1412 1138 1001 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2375 2407 2536 2568 3613 3645 7242 7274 2375
Size: 32 129 32 1045 32 3597 32 2383
Fragments arranged by size:
3597 2383 1045 129 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3538 3621 1316
Size: 2222 83 4977
Fragments arranged by size:
4977 2222 83
XbaI T'CTAG_A
Cuts at: 4618 4618
Size: 7282
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2086 2255 2086
Size: 169 7113
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7282
XmaI C'CCGG_G
Cuts at: 1870 3435 3864 3918 4607 1870
Size: 1565 429 54 689 4545
Fragments arranged by size:
4545 1565 689 429 54
XmnI GAAnn'nnTTC
Cuts at: 5411 5411
Size: 7282
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5090 276
Size: 53 83 186 936 3556 2468
Fragments arranged by size:
3556 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BaeI BbeI BfrBI BsiWI BsmBI BstZ17I
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspXI PsrI RsrII SacII SapI SexAI
SfiI SfoI SrfI SwaI