Restriction map for
pFN21AE2116
(Circular) (Six-base) MAPSORT of: pFN21AE2116.seq Check: 7932 from: 1 to: 7330
pFN21AE2116 7330 bp
With 182 enzymes: SgfI *
July 6, 2012 17:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5140 278
Size: 53 83 186 936 3604 2468
Fragments arranged by size:
3604 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2730 2832 3886 3915 4673 1937
Size: 793 102 1054 29 758 4594
Fragments arranged by size:
4594 1054 793 758 102 29
Acc65I G'GTAC_C
Cuts at: 4651 4651
Size: 7330
AclI AA'CG_TT
Cuts at: 1563 5457 5830 1563
Size: 3894 373 3063
Fragments arranged by size:
3894 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3215 5393 6777 7045 1632
Size: 1583 2178 1384 268 1917
Fragments arranged by size:
2178 1917 1583 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7133
AflIII A'CryG_T
Cuts at: 1203 3440 6229 7206 1203
Size: 2237 2789 977 1327
Fragments arranged by size:
2789 2237 1327 977
AgeI A'CCGG_T
Cuts at: 6161 6161
Size: 7330
AhdI GACnn_n'nnGTC
Cuts at: 6059 6059
Size: 7330
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7330
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7330
AlwNI CAG_nnn'CTG
Cuts at: 5073 6645 5073
Size: 1572 5758
ApaLI G'TGCA_C
Cuts at: 5387 6543 5387
Size: 1156 6174
ApoI r'AATT_y
Cuts at: 1493 2409 2857 3068 3425 3472 3498 3677 3722
Size: 916 448 211 357 47 26 179 45
Cuts at: 3722 4110 4139 4639 4865 5017 7126 1493
Size: 388 29 500 226 152 2109 1697
Fragments arranged by size:
2109 1697 916 500 448 388 357 226
211 179 152 47 45 29 26
AseI AT'TA_AT
Cuts at: 160 1032 2238 3354 5884 160
Size: 872 1206 1116 2530 1606
Fragments arranged by size:
2530 1606 1206 1116 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7330
AvaI C'yCGr_G
Cuts at: 1870 1943 2222 2838 3273 4655 1870
Size: 73 279 616 435 1382 4545
Fragments arranged by size:
4545 1382 616 435 279 73
BamHI G'GATC_C
Cuts at: 4660 5027 4660
Size: 367 6963
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2309 4651 6106 618
Size: 325 217 627 522 2342 1455 1842
Fragments arranged by size:
2342 1842 1455 627 522 325 217
BanII G_rGCy'C
Cuts at: 1391 3204 4649 1391
Size: 1813 1445 4072
Fragments arranged by size:
4072 1813 1445
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2568 2625 928
Size: 971 669 57 5633
Fragments arranged by size:
5633 971 669 57
BbvCI CC'TCA_GC
Cuts at: 2484 2484
Size: 7330
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5521 5555 1903
Size: 34 3584 34 3678
Fragments arranged by size:
3678 3584 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5222 6432 5222
Size: 1210 6120
BclI T'GATC_A
Cuts at: 1548 2976 4035 1548
Size: 1428 1059 4843
Fragments arranged by size:
4843 1428 1059
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5941 136
Size: 107 122 71 631 605 4269 1525
Fragments arranged by size:
4269 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4060 7325 4060
Size: 3265 4065
BlpI GC'TnA_GC
Cuts at: 2284 4747 2284
Size: 2463 4867
Bme1580I G_kGCm'C
Cuts at: 1790 2314 4439 5391 6547 1790
Size: 524 2125 952 1156 2573
Fragments arranged by size:
2573 2125 1156 952 524
BmgBI CAC'GTC
Cuts at: 1318 3590 1318
Size: 2272 5058
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3869 3965 5061
Size: 416 53 83 399 24 2452 96 1096
Cuts at: 5061 6019 442
Size: 958 1753
Fragments arranged by size:
2452 1753 1096 958 416 399 96 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2070 1056
Size: 1014 6316
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5990 1239
Size: 4751 2579
Bpu10I CC'TnA_GC
Cuts at: 2484 2484
Size: 7330
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2315 2623 4546 5455 6320 6618 2315
Size: 308 1923 909 865 298 3027
Fragments arranged by size:
3027 1923 909 865 308 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5993 882
Size: 617 4494 2219
Fragments arranged by size:
4494 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4605 5121 493
Size: 711 3401 516 2702
Fragments arranged by size:
3401 2702 711 516
BsaBI GATnn'nnATC
Cuts at: 2259 7324 2259
Size: 5065 2265
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5137 5519 6154 275
Size: 53 83 186 936 3604 382 635 1451
Fragments arranged by size:
3604 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2505 4552 5763 6161 6435 6582 6993 2505
Size: 2047 1211 398 274 147 411 2842
Fragments arranged by size:
2842 2047 1211 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3150 3180 1178
Size: 30 165 30 1747 30 5328
Fragments arranged by size:
5328 1747 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7330
BseYI C'CCAG_C
Cuts at: 6533 7029 6533
Size: 496 6834
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2000 2134 1163
Size: 573 264 134 6359
Fragments arranged by size:
6359 573 264 134
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4780 5541 6569 1540
Size: 275 186 2779 761 1028 2301
Fragments arranged by size:
2779 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4450 5391 5476 6547 4450
Size: 941 85 1071 5233
Fragments arranged by size:
5233 1071 941 85
BsmI GAATG_Cn'
Cuts at: 3463 4841 4934 3463
Size: 1378 93 5859
Fragments arranged by size:
5859 1378 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2314 3204 4439 4450 4649 5391 5476
Size: 399 524 890 1235 11 199 742 85
Cuts at: 5476 6547 1391
Size: 1071 2174
Fragments arranged by size:
2174 1235 1071 890 742 524 399 199
85 11
BspEI T'CCGG_A
Cuts at: 2505 4552 6993 2505
Size: 2047 2441 2842
Fragments arranged by size:
2842 2441 2047
BspHI T'CATG_A
Cuts at: 5219 6949 5219
Size: 1730 5600
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4685 844
Size: 3841 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5217 1394
Size: 429 3394 3507
Fragments arranged by size:
3507 3394 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3070 5825 5999 65
Size: 1183 1822 2755 174 1396
Fragments arranged by size:
2755 1822 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5974 6161 1700
Size: 110 4164 187 2869
Fragments arranged by size:
4164 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3507 3895 96
Size: 3411 388 3531
Fragments arranged by size:
3531 3411 388
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7330
BssSI C'ACGA_G
Cuts at: 5390 6402 5390
Size: 1012 6318
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2600 794
Size: 1806 5524
BstBI TT'CG_AA
Cuts at: 3119 6134 3119
Size: 3015 4315
BstEII G'GTnAC_C
Cuts at: 1184 4070 4222 4512 1184
Size: 2886 152 290 4002
Fragments arranged by size:
4002 2886 290 152
BstYI r'GATC_y
Cuts at: 1972 2842 4060 4660 5027 5412 5429 6881 6967
Size: 870 1218 600 367 385 17 1452 86
Cuts at: 6967 6979 7325 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1218 870 600 385 367 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 2833 3887 2833
Size: 1054 6276
BtgI C'CryG_G
Cuts at: 513 1065 2004 3906 513
Size: 552 939 1902 3937
Fragments arranged by size:
3937 1902 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2954 487
Size: 683 56 1728 4863
Fragments arranged by size:
4863 1728 683 56
BtsI GCAGTG_nn'
Cuts at: 4846 5640 5660 4846
Size: 794 20 6516
Fragments arranged by size:
6516 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7330
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3135 3170 529
Size: 35 2571 35 4689
Fragments arranged by size:
4689 2571 35 35
DraI TTT'AAA
Cuts at: 2371 3990 4634 2371
Size: 1619 644 5067
Fragments arranged by size:
5067 1619 644
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6337 809
Size: 507 5021 1802
Fragments arranged by size:
5021 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4777 5666 7295
Size: 54 1425 50 275 18 2947 889 1629
Cuts at: 7295 8
Size: 43
Fragments arranged by size:
2947 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4777 1537
Size: 275 2965 4090
Fragments arranged by size:
4090 2965 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2388 2461 2550 3192 3339 4421 5260 1353
Size: 1035 73 89 642 147 1082 839 3423
Fragments arranged by size:
3423 1082 1035 839 642 147 89 73
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5917 6291 6437 387
Size: 1064 138 4328 374 146 1280
Fragments arranged by size:
4328 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3215 5393 5990 6777 7045 1239
Size: 393 1583 2178 597 787 268 1524
Fragments arranged by size:
2178 1583 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 4455 4455
Size: 7330
EcoO109I rG'GnC_Cy
Cuts at: 2687 3269 2687
Size: 582 6748
EcoRI G'AATT_C
Cuts at: 3498 4639 5017 3498
Size: 1141 378 5811
Fragments arranged by size:
5811 1141 378
EcoRV GAT'ATC
Cuts at: 4050 4050
Size: 7330
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2304 4592 2304
Size: 2288 5042
FspI TGC'GCA
Cuts at: 5836 5836
Size: 7330
HaeII r_GCGC'y
Cuts at: 1678 2358 6477 1678
Size: 680 4119 2531
Fragments arranged by size:
4119 2531 680
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2684 2716 4072 4104 5971
Size: 32 135 32 1112 32 1356 32 1867
Cuts at: 5971 6003 6045 6077 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1867 1356 1112 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2124 4674 4920 669
Size: 988 281 186 2550 246 3079
Fragments arranged by size:
3079 2550 988 281 246 186
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7330
HpaI GTT'AAC
Cuts at: 2124 4920 2124
Size: 2796 4534
KpnI G_GTAC'C
Cuts at: 4655 4655
Size: 7330
MfeI C'AATT_G
Cuts at: 4929 4929
Size: 7330
MluI A'CGCG_T
Cuts at: 7206 7206
Size: 7330
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2201 6444 6628 1305
Size: 896 4243 184 2007
Fragments arranged by size:
4243 2007 896 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5508
Fragments arranged by size:
5508 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5288 5647 5806 518
Size: 648 4122 359 159 2042
Fragments arranged by size:
4122 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2087 3733 4746 5423 6571 6816 1606
Size: 481 1646 1013 677 1148 245 2120
Fragments arranged by size:
2120 1646 1148 1013 677 481 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3906 513
Size: 552 939 1902 3937
Fragments arranged by size:
3937 1902 939 552
NdeI CA'TA_TG
Cuts at: 387 4218 387
Size: 3831 3499
NheI G'CTAG_C
Cuts at: 1052 2066 1052
Size: 1014 6316
NotI GC'GGCC_GC
Cuts at: 4777 4777
Size: 7330
NspI r_CATG'y
Cuts at: 1132 1229 2598 3444 3508 4627 4688 6233 1132
Size: 97 1369 846 64 1119 61 1545 2229
Fragments arranged by size:
2229 1545 1369 1119 846 97 64 61
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7330
PciI A'CATG_T
Cuts at: 3440 6229 3440
Size: 2789 4541
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3764 1108
Size: 2656 4674
PmeI GTTT'AAAC
Cuts at: 4634 4634
Size: 7330
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3413 3445 5399 5431 6912 6944 3413
Size: 32 1954 32 1481 32 3799
Fragments arranged by size:
3799 1954 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3269 3269
Size: 7330
PshAI GACnn'nnGTC
Cuts at: 1613 2986 1613
Size: 1373 5957
PsiI TTA'TAA
Cuts at: 2232 3051 4900 2232
Size: 819 1849 4662
Fragments arranged by size:
4662 1849 819
PspXI vC'TCGA_Gb
Cuts at: 2222 3273 2222
Size: 1051 6279
PstI C_TGCA'G
Cuts at: 830 3652 4682 830
Size: 2822 1030 3478
Fragments arranged by size:
3478 2822 1030
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7330
PvuII CAG'CTG
Cuts at: 2087 3733 2087
Size: 1646 5684
SalI G'TCGA_C
Cuts at: 1936 4672 1936
Size: 2736 4594
SapI GCTCTTCn'nnn_
Cuts at: 3192 3192
Size: 7330
SbfI CC_TGCA'GG
Cuts at: 4682 4682
Size: 7330
ScaI AGT'ACT
Cuts at: 3858 4247 5578 7290 3858
Size: 389 1331 1712 3898
Fragments arranged by size:
3898 1712 1331 389
SfcI C'TryA_G
Cuts at: 826 1045 3535 3648 3916 4323 4678 5813 6494
Size: 219 2490 113 268 407 355 1135 681
Cuts at: 6494 6685 826
Size: 191 1471
Fragments arranged by size:
2490 1471 1135 681 407 355 268 219
191 113
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7330
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7330
SmaI CCC'GGG
Cuts at: 1872 4657 1872
Size: 2785 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2222 2294 2638 2838 3273 4561
Size: 197 926 279 72 344 200 435 1288
Cuts at: 4561 5434 6335 6597 820
Size: 873 901 262 1553
Fragments arranged by size:
1553 1288 926 901 873 435 344 279
262 200 197 72
SnaBI TAC'GTA
Cuts at: 493 5121 493
Size: 4628 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7330
SphI G_CATG'C
Cuts at: 1132 4627 4688 1132
Size: 3495 61 3774
Fragments arranged by size:
3774 3495 61
SspI AAT'ATT
Cuts at: 5 52 5254 5
Size: 47 5202 2081
Fragments arranged by size:
5202 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7330
StyI C'CwwG_G
Cuts at: 513 1065 2004 2526 2965 3906 4769 513
Size: 552 939 522 439 941 863 3074
Fragments arranged by size:
3074 941 939 863 552 522 439
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3984 5357 5374 5527 5712 6157 325
Size: 1255 2404 1373 17 153 185 445 1498
Fragments arranged by size:
2404 1498 1373 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3507 3856 3895
Size: 274 80 33 51 1443 1530 349 39
Cuts at: 3895 4245 5576 7288 96
Size: 350 1331 1712 138
Fragments arranged by size:
1712 1530 1443 1331 350 349 274 138
80 51 39 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3150 3182 7290 7322 3150
Size: 32 4108 32 3158
Fragments arranged by size:
4108 3158 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7330
XbaI T'CTAG_A
Cuts at: 3376 4297 4666 3376
Size: 921 369 6040
Fragments arranged by size:
6040 921 369
XhoI C'TCGA_G
Cuts at: 1943 2222 2838 3273 1943
Size: 279 616 435 6000
Fragments arranged by size:
6000 616 435 279
XmaI C'CCGG_G
Cuts at: 1870 4655 1870
Size: 2785 4545
XmnI GAAnn'nnTTC
Cuts at: 2861 3425 3726 4383 5459 2861
Size: 564 301 657 1076 4732
Fragments arranged by size:
4732 1076 657 564 301
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5138 276
Size: 53 83 186 936 3604 2468
Fragments arranged by size:
3604 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspXI PstI PvuI PvuII SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspOMI PsrI RsrII SacI SacII
SanDI SexAI SfiI SfoI SrfI SwaI XcmI