Restriction map for
pFN21AE2106
(Circular) (Six-base) MAPSORT of: pFN21AE2106.seq Check: 5823 from: 1 to: 7387
pFN21AE2106 7387 bp
With 182 enzymes: SgfI *
August 16, 2010 14:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5197 278
Size: 53 83 186 936 3661 2468
Fragments arranged by size:
3661 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4730 1937
Size: 2793 4594
Acc65I G'GTAC_C
Cuts at: 4708 4708
Size: 7387
AclI AA'CG_TT
Cuts at: 1563 5514 5887 1563
Size: 3951 373 3063
Fragments arranged by size:
3951 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2185 2382 2718 4058 5450 6834 7102 1632
Size: 553 197 336 1340 1392 1384 268 1917
Fragments arranged by size:
1917 1392 1384 1340 553 336 268 197
AfeI AGC'GCT
Cuts at: 3197 3197
Size: 7387
AflII C'TTAA_G
Cuts at: 820 1017 2213 820
Size: 197 1196 5994
Fragments arranged by size:
5994 1196 197
AflIII A'CryG_T
Cuts at: 1203 2040 6286 7263 1203
Size: 837 4246 977 1327
Fragments arranged by size:
4246 1327 977 837
AgeI A'CCGG_T
Cuts at: 6218 6218
Size: 7387
AhdI GACnn_n'nnGTC
Cuts at: 6116 6116
Size: 7387
AleI CACnn'nnGTG
Cuts at: 1166 4164 1166
Size: 2998 4389
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7387
AlwNI CAG_nnn'CTG
Cuts at: 3136 3664 4231 4344 5130 6702 3136
Size: 528 567 113 786 1572 3821
Fragments arranged by size:
3821 1572 786 567 528 113
ApaI G_GGCC'C
Cuts at: 3957 4619 3957
Size: 662 6725
ApaLI G'TGCA_C
Cuts at: 4635 5444 6600 4635
Size: 809 1156 5422
Fragments arranged by size:
5422 1156 809
ApoI r'AATT_y
Cuts at: 1493 3459 4382 4696 4922 5074 7183 1493
Size: 1966 923 314 226 152 2109 1697
Fragments arranged by size:
2109 1966 1697 923 314 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5941 160
Size: 872 4909 1606
Fragments arranged by size:
4909 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7387
AvaI C'yCGr_G
Cuts at: 1870 1943 2930 3701 4612 4712 1870
Size: 73 987 771 911 100 4545
Fragments arranged by size:
4545 987 911 771 100 73
BamHI G'GATC_C
Cuts at: 3960 4512 4717 5084 3960
Size: 552 205 367 6263
Fragments arranged by size:
6263 552 367 205
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2171 3122 3450 3921 4424
Size: 325 217 627 384 951 328 471 503
Cuts at: 4424 4708 6163 618
Size: 284 1455 1842
Fragments arranged by size:
1842 1455 951 627 503 471 384 328
325 284 217
BanII G_rGCy'C
Cuts at: 1391 2022 3322 3408 3825 3957 3995 4015 4619
Size: 631 1300 86 417 132 38 20 604
Cuts at: 4619 4706 1391
Size: 87 4072
Fragments arranged by size:
4072 1300 631 604 417 132 87 86
38 20
BbeI G_GCGC'C
Cuts at: 3925 3925
Size: 7387
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2135 2202 2706 2942 4026 928
Size: 971 236 67 504 236 1084 4289
Fragments arranged by size:
4289 1084 971 504 236 236 67
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5578 5612 1903
Size: 34 3641 34 3678
Fragments arranged by size:
3678 3641 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5279 6489 5279
Size: 1210 6177
BclI T'GATC_A
Cuts at: 1548 3547 3666 1548
Size: 1999 119 5269
Fragments arranged by size:
5269 1999 119
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3575 5998 136
Size: 107 122 71 631 605 1903 2423 1525
Fragments arranged by size:
2423 1903 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3270 7382 3270
Size: 4112 3275
BlpI GC'TnA_GC
Cuts at: 4804 4804
Size: 7387
Bme1580I G_kGCm'C
Cuts at: 1790 2569 3861 3957 4619 4639 5448 6604 1790
Size: 779 1292 96 662 20 809 1156 2573
Fragments arranged by size:
2573 1292 1156 809 779 662 96 20
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7387
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3491 4576 5118
Size: 416 53 83 399 24 2074 1085 542
Cuts at: 5118 6076 442
Size: 958 1753
Fragments arranged by size:
2074 1753 1085 958 542 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2873 1056
Size: 1817 5570
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3597 3597
Size: 7387
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2936 2984 3047 3158 3212 3581 4109 6047
Size: 1697 48 63 111 54 369 528 1938
Cuts at: 6047 1239
Size: 2579
Fragments arranged by size:
2579 1938 1697 528 369 111 63 54
48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3772 5512 6377 6675 3772
Size: 1740 865 298 4484
Fragments arranged by size:
4484 1740 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4089 6050 882
Size: 617 2590 1961 2219
Fragments arranged by size:
2590 2219 1961 617
BsaAI yAC'GTr
Cuts at: 493 1204 2041 5178 493
Size: 711 837 3137 2702
Fragments arranged by size:
3137 2702 837 711
BsaBI GATnn'nnATC
Cuts at: 7381 7381
Size: 7387
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3299 3922 5194 5576
Size: 53 83 186 936 1766 623 1272 382
Cuts at: 5576 6211 275
Size: 635 1451
Fragments arranged by size:
1766 1451 1272 936 635 623 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2922 3825 4509 5820 6218 6492 6639 7050 2922
Size: 903 684 1311 398 274 147 411 3259
Fragments arranged by size:
3259 1311 903 684 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2063 2093 1178
Size: 30 165 30 660 30 6472
Fragments arranged by size:
6472 660 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2087 2090 3176 3517 3665 3899 3992 4562
Size: 901 3 1086 341 148 234 93 570
Cuts at: 4562 1186
Size: 4011
Fragments arranged by size:
4011 1086 901 570 341 234 148 93
3
BseYI C'CCAG_C
Cuts at: 2104 6590 7086 2104
Size: 4486 496 2405
Fragments arranged by size:
4486 2405 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2048 3029 1163
Size: 573 312 981 5521
Fragments arranged by size:
5521 981 573 312
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4837 5598 6626 1540
Size: 275 186 2836 761 1028 2301
Fragments arranged by size:
2836 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3408 3825 3995 4053 4161 4639 5448 5533 6604
Size: 417 170 58 108 478 809 85 1071
Cuts at: 6604 3408
Size: 4191
Fragments arranged by size:
4191 1071 809 478 417 170 108 85
58
BsiWI C'GTAC_G
Cuts at: 2745 2745
Size: 7387
BsmI GAATG_Cn'
Cuts at: 4898 4991 4898
Size: 93 7294
BsmBI CGTCTCn'nnnn_
Cuts at: 2755 4460 2755
Size: 1705 5682
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2022 2569 3322 3408 3825 3861 3957
Size: 399 232 547 753 86 417 36 96
Cuts at: 3957 3995 4015 4053 4161 4619 4639 4706 5448
Size: 38 20 38 108 458 20 67 742
Cuts at: 5448 5533 6604 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 753 742 547 458 417 399
232 108 96 86 85 67 38 38
36 20 20
BspEI T'CCGG_A
Cuts at: 3825 4509 7050 3825
Size: 684 2541 4162
Fragments arranged by size:
4162 2541 684
BspHI T'CATG_A
Cuts at: 2739 4327 4455 5276 7006 2739
Size: 1588 128 821 1730 3120
Fragments arranged by size:
3120 1730 1588 821 128
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2100 4483 4742 844
Size: 1256 2383 259 3489
Fragments arranged by size:
3489 2383 1256 259
BsrBI CCG'CTC
Cuts at: 1394 1823 3718 5274 1394
Size: 429 1895 1556 3507
Fragments arranged by size:
3507 1895 1556 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2386 5882 6056 65
Size: 1183 1138 3496 174 1396
Fragments arranged by size:
3496 1396 1183 1138 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2168 2583 6031 6218 1700
Size: 110 358 415 3448 187 2869
Fragments arranged by size:
3448 2869 415 358 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7387
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7387
BssSI C'ACGA_G
Cuts at: 3242 3335 3854 5447 6459 3242
Size: 93 519 1593 1012 4170
Fragments arranged by size:
4170 1593 1012 519 93
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2950 3274 3856 794
Size: 2156 324 582 4325
Fragments arranged by size:
4325 2156 582 324
BstBI TT'CG_AA
Cuts at: 6191 6191
Size: 7387
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7387
BstYI r'GATC_y
Cuts at: 1972 3270 3616 3654 3960 4227 4408 4512 4717
Size: 1298 346 38 306 267 181 104 205
Cuts at: 4717 5084 5469 5486 6938 7024 7036 7382 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1298 385 367 346 346 306
267 205 181 104 86 38 17 12
Bsu36I CC'TnA_GG
Cuts at: 2159 2159
Size: 7387
BtgI C'CryG_G
Cuts at: 513 1065 2649 3813 4356 513
Size: 552 1584 1164 543 3544
Fragments arranged by size:
3544 1584 1164 552 543
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3379 4180 4483 487
Size: 683 56 2153 801 303 3391
Fragments arranged by size:
3391 2153 801 683 303 56
BtsI GCAGTG_nn'
Cuts at: 2136 3669 4482 4630 4903 5697 5717 2136
Size: 1533 813 148 273 794 20 3806
Fragments arranged by size:
3806 1533 813 794 273 148 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7387
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7352
DraI TTT'AAA
Cuts at: 4691 4691
Size: 7387
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6394 809
Size: 507 5078 1802
Fragments arranged by size:
5078 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2646 3732 4834
Size: 54 1425 50 275 18 816 1086 1102
Cuts at: 4834 5723 7352 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1102 1086 889 816 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4834 1537
Size: 275 3022 4090
Fragments arranged by size:
4090 3022 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3494 4663 5317 1353
Size: 2141 1169 654 3423
Fragments arranged by size:
3423 2141 1169 654
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3722 4559 5974 6348 6494 387
Size: 1064 138 2133 837 1415 374 146 1280
Fragments arranged by size:
2133 1415 1280 1064 837 374 146 138
EcoICRI GAG'CTC
Cuts at: 3406 3823 3993 3406
Size: 417 170 6800
Fragments arranged by size:
6800 417 170
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2185 2382 2718 2936 2984 3047 3158
Size: 393 553 197 336 218 48 63 111
Cuts at: 3158 3212 3581 4058 4109 5450 6047 6834 7102
Size: 54 369 477 51 1341 597 787 268
Cuts at: 7102 1239
Size: 1524
Fragments arranged by size:
1524 1341 787 597 553 477 393 369
336 268 218 197 111 63 54 51
48
EcoNI CCTnn'n_nnAGG
Cuts at: 3130 3881 4544 3130
Size: 751 663 5973
Fragments arranged by size:
5973 751 663
EcoO109I rG'GnC_Cy
Cuts at: 3954 4117 3954
Size: 163 7224
EcoRI G'AATT_C
Cuts at: 4696 5074 4696
Size: 378 7009
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2570 2570
Size: 7387
FspI TGC'GCA
Cuts at: 3675 5893 3675
Size: 2218 5169
HaeII r_GCGC'y
Cuts at: 1678 2361 3199 3609 3925 6534 1678
Size: 683 838 410 316 2609 2531
Fragments arranged by size:
2609 2531 838 683 410 316
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3278 3284 3310 3316 3646
Size: 32 135 32 1706 6 26 6 330
Cuts at: 3646 3678 3707 3739 6028 6060 6102 6134 1373
Size: 32 29 32 2289 32 42 32 2626
Fragments arranged by size:
2626 2289 1706 330 135 42 32 32
32 32 32 32 29 26 6 6
HincII GTy'rAC
Cuts at: 669 1657 1938 2305 4731 4977 669
Size: 988 281 367 2426 246 3079
Fragments arranged by size:
3079 2426 988 367 281 246
HindIII A'AGCT_T
Cuts at: 748 2310 2609 748
Size: 1562 299 5526
Fragments arranged by size:
5526 1562 299
HpaI GTT'AAC
Cuts at: 4977 4977
Size: 7387
KasI G'GCGC_C
Cuts at: 3921 3921
Size: 7387
KpnI G_GTAC'C
Cuts at: 4712 4712
Size: 7387
MfeI C'AATT_G
Cuts at: 3592 4986 3592
Size: 1394 5993
MluI A'CGCG_T
Cuts at: 7263 7263
Size: 7387
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2670 3090 4150 6501 6685 1305
Size: 1365 420 1060 2351 184 2007
Fragments arranged by size:
2351 2007 1365 1060 420 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2648 3734 10
Size: 54 1425 343 816 1086 3663
Fragments arranged by size:
3663 1425 1086 816 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4164 5345 5704 5863 518
Size: 648 2998 1181 359 159 2042
Fragments arranged by size:
2998 2042 1181 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2798 3025 4036 4803 5480 6628 6873 1606
Size: 1192 227 1011 767 677 1148 245 2120
Fragments arranged by size:
2120 1192 1148 1011 767 677 245 227
NaeI GCC'GGC
Cuts at: 2170 2170
Size: 7387
NarI GG'CG_CC
Cuts at: 3922 3922
Size: 7387
NcoI C'CATG_G
Cuts at: 513 1065 2649 3813 4356 513
Size: 552 1584 1164 543 3544
Fragments arranged by size:
3544 1584 1164 552 543
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7387
NgoMIV G'CCGG_C
Cuts at: 2168 2168
Size: 7387
NheI G'CTAG_C
Cuts at: 1052 2869 1052
Size: 1817 5570
NotI GC'GGCC_GC
Cuts at: 4834 4834
Size: 7387
NspI r_CATG'y
Cuts at: 1132 1229 3905 4745 6290 1132
Size: 97 2676 840 1545 2229
Fragments arranged by size:
2676 2229 1545 840 97
PasI CC'CwG_GG
Cuts at: 1348 2395 1348
Size: 1047 6340
PciI A'CATG_T
Cuts at: 6286 6286
Size: 7387
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7387
PfoI T'CCnGG_A
Cuts at: 2067 2097 2973 4110 2067
Size: 30 876 1137 5344
Fragments arranged by size:
5344 1137 876 30
PmeI GTTT'AAAC
Cuts at: 4691 4691
Size: 7387
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3478 3510 5456 5488 6969 7001 3478
Size: 32 1946 32 1481 32 3864
Fragments arranged by size:
3864 1946 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2747 1613
Size: 1134 6253
PsiI TTA'TAA
Cuts at: 4957 4957
Size: 7387
PspOMI G'GGCC_C
Cuts at: 3953 4615 3953
Size: 662 6725
PstI C_TGCA'G
Cuts at: 830 3051 3282 3882 4739 830
Size: 2221 231 600 857 3478
Fragments arranged by size:
3478 2221 857 600 231
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7387
PvuII CAG'CTG
Cuts at: 3025 4036 3025
Size: 1011 6376
SacI G_AGCT'C
Cuts at: 3408 3825 3995 3408
Size: 417 170 6800
Fragments arranged by size:
6800 417 170
SalI G'TCGA_C
Cuts at: 1936 4729 1936
Size: 2793 4594
SbfI CC_TGCA'GG
Cuts at: 3882 4739 3882
Size: 857 6530
ScaI AGT'ACT
Cuts at: 4586 5635 7347 4586
Size: 1049 1712 4626
Fragments arranged by size:
4626 1712 1049
SfcI C'TryA_G
Cuts at: 826 1045 3047 3278 3878 4735 5870 6551 6742
Size: 219 2002 231 600 857 1135 681 191
Cuts at: 6742 826
Size: 1471
Fragments arranged by size:
2002 1471 1135 857 681 600 231 219
191
SfoI GGC'GCC
Cuts at: 3923 3923
Size: 7387
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7387
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7387
SmaI CCC'GGG
Cuts at: 1872 4714 1872
Size: 2842 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2213 3787 5491 6392 6654 820
Size: 197 926 270 1574 1704 901 262 1553
Fragments arranged by size:
1704 1574 1553 926 901 270 262 197
SnaBI TAC'GTA
Cuts at: 493 5178 493
Size: 4685 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7387
SphI G_CATG'C
Cuts at: 1132 3905 4745 1132
Size: 2773 840 3774
Fragments arranged by size:
3774 2773 840
SspI AAT'ATT
Cuts at: 5 52 2638 5311 5
Size: 47 2586 2673 2081
Fragments arranged by size:
2673 2586 2081 47
StuI AGG'CCT
Cuts at: 1517 3233 1517
Size: 1716 5671
StyI C'CwwG_G
Cuts at: 513 1065 2554 2649 3003 3813 4356 4826 513
Size: 552 1489 95 354 810 543 470 3074
Fragments arranged by size:
3074 1489 810 552 543 470 354 95
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2189 3016 3492 4271 4361 5414 5431
Size: 1255 609 827 476 779 90 1053 17
Cuts at: 5431 5584 5769 6214 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1053 827 779 609 476 445
185 153 90 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2031 2058 4584
Size: 274 80 33 51 1443 54 27 2526
Cuts at: 4584 5633 7345 96
Size: 1049 1712 138
Fragments arranged by size:
2526 1712 1443 1049 274 138 80 54
51 33 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2063 2095 7347 7379 2063
Size: 32 5252 32 2071
Fragments arranged by size:
5252 2071 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7387
XbaI T'CTAG_A
Cuts at: 4723 4723
Size: 7387
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4213 4213
Size: 7387
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7387
XmaI C'CCGG_G
Cuts at: 1870 4712 1870
Size: 2842 4545
XmnI GAAnn'nnTTC
Cuts at: 2132 2892 4274 5516 2132
Size: 760 1382 1242 4003
Fragments arranged by size:
4003 1382 1242 760
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5195 276
Size: 53 83 186 936 3661 2468
Fragments arranged by size:
3661 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BstXI BstZ17I DraIII EcoRV FseI FspAI NruI NsiI
PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SrfI SwaI