Restriction map for
pFN21AE2103
(Circular) (Six-base) MAPSORT of: pFN21AE2103.seq Check: 2382 from: 1 to: 7471
pFN21AE2103 7471 bp
With 182 enzymes: SgfI *
March 28, 2012 12:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2729 2729
Size: 7471
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2151 5281 278
Size: 53 83 186 936 615 3130 2468
Fragments arranged by size:
3130 2468 936 615 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2634 2669 4692 4708 4814 1937
Size: 697 35 2023 16 106 4594
Fragments arranged by size:
4594 2023 697 106 35 16
Acc65I G'GTAC_C
Cuts at: 4792 4792
Size: 7471
AclI AA'CG_TT
Cuts at: 1563 5598 5971 1563
Size: 4035 373 3063
Fragments arranged by size:
4035 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3371 3494 5534 6918 7186 1632
Size: 1739 123 2040 1384 268 1917
Fragments arranged by size:
2040 1917 1739 1384 268 123
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7274
AflIII A'CryG_T
Cuts at: 1203 4310 4394 6370 7347 1203
Size: 3107 84 1976 977 1327
Fragments arranged by size:
3107 1976 1327 977 84
AgeI A'CCGG_T
Cuts at: 6302 6302
Size: 7471
AhdI GACnn_n'nnGTC
Cuts at: 6200 6200
Size: 7471
AleI CACnn'nnGTG
Cuts at: 1166 3395 1166
Size: 2229 5242
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2907 3362 1184
Size: 1723 455 5293
Fragments arranged by size:
5293 1723 455
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4554 4586 4554
Size: 32 7439
AlwNI CAG_nnn'CTG
Cuts at: 2827 5214 6786 2827
Size: 2387 1572 3512
Fragments arranged by size:
3512 2387 1572
ApaLI G'TGCA_C
Cuts at: 2174 3571 5528 6684 2174
Size: 1397 1957 1156 2961
Fragments arranged by size:
2961 1957 1397 1156
ApoI r'AATT_y
Cuts at: 1493 4351 4780 5006 5158 7267 1493
Size: 2858 429 226 152 2109 1697
Fragments arranged by size:
2858 2109 1697 429 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6025 160
Size: 872 4993 1606
Fragments arranged by size:
4993 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7471
AvaI C'yCGr_G
Cuts at: 1870 1943 2156 2555 3472 4335 4346 4796 1870
Size: 73 213 399 917 863 11 450 4545
Fragments arranged by size:
4545 917 863 450 399 213 73 11
AvrII C'CTAG_G
Cuts at: 2650 2650
Size: 7471
BamHI G'GATC_C
Cuts at: 2229 2316 2954 4801 5168 2229
Size: 87 638 1847 367 4532
Fragments arranged by size:
4532 1847 638 367 87
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2551 2599 4433 4792 6247
Size: 325 217 627 764 48 1834 359 1455
Cuts at: 6247 618
Size: 1842
Fragments arranged by size:
1842 1834 1455 764 627 359 325 217
48
BanII G_rGCy'C
Cuts at: 1391 4680 4790 1391
Size: 3289 110 4072
Fragments arranged by size:
4072 3289 110
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3162 3531 3611 3781 928
Size: 971 1263 369 80 170 4618
Fragments arranged by size:
4618 1263 971 369 170 80
BbvCI CC'TCA_GC
Cuts at: 2015 2015
Size: 7471
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3711 3745 5662 5696 1903
Size: 34 1774 34 1917 34 3678
Fragments arranged by size:
3678 1917 1774 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5363 6573 5363
Size: 1210 6261
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7471
BfrBI ATG'CAT
Cuts at: 2543 2543
Size: 7471
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6082 136
Size: 107 122 71 631 605 4410 1525
Fragments arranged by size:
4410 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4261 7466 4261
Size: 3205 4266
BlpI GC'TnA_GC
Cuts at: 4888 4888
Size: 7471
Bme1580I G_kGCm'C
Cuts at: 1790 2178 2202 3575 5532 6688 1790
Size: 388 24 1373 1957 1156 2573
Fragments arranged by size:
2573 1957 1373 1156 388 24
BmgBI CAC'GTC
Cuts at: 1318 2404 1318
Size: 1086 6385
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2752 3115 4638
Size: 416 53 83 399 24 1335 363 1523
Cuts at: 4638 5202 6160 442
Size: 564 958 1753
Fragments arranged by size:
1753 1523 1335 958 564 416 399 363
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7471
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3781 4259 3781
Size: 478 6993
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2087 2827 3354 3378 3776 6131 1239
Size: 848 740 527 24 398 2355 2579
Fragments arranged by size:
2579 2355 848 740 527 398 24
Bpu10I CC'TnA_GC
Cuts at: 2015 4224 2015
Size: 2209 5262
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4229 5596 6461 6759 4229
Size: 1367 865 298 4941
Fragments arranged by size:
4941 1367 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2491 6134 882
Size: 617 992 3643 2219
Fragments arranged by size:
3643 2219 992 617
BsaAI yAC'GTr
Cuts at: 493 1204 5262 493
Size: 711 4058 2702
Fragments arranged by size:
4058 2702 711
BsaBI GATnn'nnATC
Cuts at: 7465 7465
Size: 7471
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2148 5278 5660 6295
Size: 53 83 186 936 615 3130 382 635
Cuts at: 6295 275
Size: 1451
Fragments arranged by size:
3130 1451 936 635 615 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2133 3725 5904 6302 6576 6723 7134 2133
Size: 1592 2179 398 274 147 411 2470
Fragments arranged by size:
2470 2179 1592 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7246
Fragments arranged by size:
7246 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7471
BseYI C'CCAG_C
Cuts at: 2189 2305 2949 4513 6674 7170 2189
Size: 116 644 1564 2161 496 2490
Fragments arranged by size:
2490 2161 1564 644 496 116
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2395 2957 1163
Size: 573 659 562 5677
Fragments arranged by size:
5677 659 573 562
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4921 5682 6710 1540
Size: 275 186 2920 761 1028 2301
Fragments arranged by size:
2920 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2076 2178 3575 4680 5532 5617 6688 2076
Size: 102 1397 1105 852 85 1071 2859
Fragments arranged by size:
2859 1397 1105 1071 852 102 85
BsmI GAATG_Cn'
Cuts at: 4293 4982 5075 4293
Size: 689 93 6689
Fragments arranged by size:
6689 689 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2076 2178 2202 3575 4680 4790 5532
Size: 399 286 102 24 1373 1105 110 742
Cuts at: 5532 5617 6688 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1373 1105 1071 742 399 286 110
102 85 24
BspEI T'CCGG_A
Cuts at: 2133 7134 2133
Size: 5001 2470
BspHI T'CATG_A
Cuts at: 5360 7090 5360
Size: 1730 5741
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2729 4826 844
Size: 1885 2097 3489
Fragments arranged by size:
3489 2097 1885
BsrBI CCG'CTC
Cuts at: 1394 1823 4439 5358 1394
Size: 429 2616 919 3507
Fragments arranged by size:
3507 2616 919 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2751 3370 3688 3804 3810 5966 6140
Size: 1183 1503 619 318 116 6 2156 174
Cuts at: 6140 65
Size: 1396
Fragments arranged by size:
2156 1503 1396 1183 619 318 174 116
6
BsrFI r'CCGG_y
Cuts at: 1700 1810 2428 2571 6115 6302 1700
Size: 110 618 143 3544 187 2869
Fragments arranged by size:
3544 2869 618 187 143 110
BsrGI T'GTAC_A
Cuts at: 96 4386 4539 4729 96
Size: 4290 153 190 2838
Fragments arranged by size:
4290 2838 190 153
BssHII G'CGCG_C
Cuts at: 1925 2082 2099 2310 1925
Size: 157 17 211 7086
Fragments arranged by size:
7086 211 157 17
BssSI C'ACGA_G
Cuts at: 5531 6543 5531
Size: 1012 6459
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3251 794
Size: 2457 5014
BstBI TT'CG_AA
Cuts at: 6275 6275
Size: 7471
BstEII G'GTnAC_C
Cuts at: 1184 3476 3653 1184
Size: 2292 177 5002
Fragments arranged by size:
5002 2292 177
BstYI r'GATC_y
Cuts at: 1972 2229 2316 2523 2886 2954 3286 4261 4801
Size: 257 87 207 363 68 332 975 540
Cuts at: 4801 5168 5553 5570 7022 7108 7120 7466 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 975 540 385 367 363 346
332 257 207 87 86 68 17 12
BstZ17I GTA'TAC
Cuts at: 4709 4709
Size: 7471
Bsu36I CC'TnA_GG
Cuts at: 2510 2510
Size: 7471
BtgI C'CryG_G
Cuts at: 513 1065 2004 2628 3027 513
Size: 552 939 624 399 4957
Fragments arranged by size:
4957 939 624 552 399
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2261 3663 487
Size: 683 56 1035 1402 4295
Fragments arranged by size:
4295 1402 1035 683 56
BtsI GCAGTG_nn'
Cuts at: 2969 3500 3539 3969 4987 5781 5801 2969
Size: 531 39 430 1018 794 20 4639
Fragments arranged by size:
4639 1018 794 531 430 39 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7471
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3805 3840 529
Size: 35 3241 35 4160
Fragments arranged by size:
4160 3241 35 35
DraI TTT'AAA
Cuts at: 4775 4775
Size: 7471
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6478 809
Size: 507 5162 1802
Fragments arranged by size:
5162 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2104 2920 3831
Size: 54 1425 50 275 18 274 816 911
Cuts at: 3831 4918 5807 7436 8
Size: 1087 889 1629 43
Fragments arranged by size:
1629 1425 1087 911 889 816 275 274
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4918 1537
Size: 275 3106 4090
Fragments arranged by size:
4090 3106 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2522 5401 1353
Size: 1169 2879 3423
Fragments arranged by size:
3423 2879 1169
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6058 6432 6578 387
Size: 1064 138 4469 374 146 1280
Fragments arranged by size:
4469 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4678 4678
Size: 7471
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2087 2827 3354 3371 3378 3494 3776
Size: 393 455 740 527 17 7 116 282
Cuts at: 3776 5534 6131 6918 7186 1239
Size: 1758 597 787 268 1524
Fragments arranged by size:
1758 1524 787 740 597 527 455 393
282 268 116 17 7
EcoO109I rG'GnC_Cy
Cuts at: 2186 2647 2186
Size: 461 7010
EcoRI G'AATT_C
Cuts at: 4780 5158 4780
Size: 378 7093
EcoRV GAT'ATC
Cuts at: 2989 2989
Size: 7471
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4606 4606
Size: 7471
FspI TGC'GCA
Cuts at: 5977 5977
Size: 7471
HaeII r_GCGC'y
Cuts at: 1678 2381 2578 4514 6618 1678
Size: 703 197 1936 2104 2531
Fragments arranged by size:
2531 2104 1936 703 197
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2573 2605 6112 6144 6186
Size: 32 135 32 1001 32 3507 32 42
Cuts at: 6186 6218 1373
Size: 32 2626
Fragments arranged by size:
3507 2626 1001 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3132 4815 5061 669
Size: 988 281 1194 1683 246 3079
Fragments arranged by size:
3079 1683 1194 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4588 748
Size: 3840 3631
HpaI GTT'AAC
Cuts at: 5061 5061
Size: 7471
KpnI G_GTAC'C
Cuts at: 4796 4796
Size: 7471
MfeI C'AATT_G
Cuts at: 5070 5070
Size: 7471
MluI A'CGCG_T
Cuts at: 7347 7347
Size: 7471
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2424 2437 3228 3611 6585 6769 1305
Size: 1119 13 791 383 2974 184 2007
Fragments arranged by size:
2974 2007 1119 791 383 184 13
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2922 3833 10
Size: 54 1425 343 1090 911 3648
Fragments arranged by size:
3648 1425 1090 911 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2979 3395 3580 5429 5788 5947 518
Size: 648 1813 416 185 1849 359 159 2042
Fragments arranged by size:
2042 1849 1813 648 416 359 185 159
MspA1I CmG'CkG
Cuts at: 1606 2014 2193 2949 4448 4887 5564 6712 6957
Size: 408 179 756 1499 439 677 1148 245
Cuts at: 6957 1606
Size: 2120
Fragments arranged by size:
2120 1499 1148 756 677 439 408 245
179
NcoI C'CATG_G
Cuts at: 513 1065 2004 2628 513
Size: 552 939 624 5356
Fragments arranged by size:
5356 939 624 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7471
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7471
NotI GC'GGCC_GC
Cuts at: 4918 4918
Size: 7471
NsiI A_TGCA'T
Cuts at: 2545 2545
Size: 7471
NspI r_CATG'y
Cuts at: 1132 1229 3532 3684 4314 4398 4531 4829 6374
Size: 97 2303 152 630 84 133 298 1545
Cuts at: 6374 1132
Size: 2229
Fragments arranged by size:
2303 2229 1545 630 298 152 133 97
84
PasI CC'CwG_GG
Cuts at: 1348 3771 1348
Size: 2423 5048
PciI A'CATG_T
Cuts at: 4310 4394 6370 4310
Size: 84 1976 5411
Fragments arranged by size:
5411 1976 84
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2239 2629 1108
Size: 1131 390 5950
Fragments arranged by size:
5950 1131 390
PfoI T'CCnGG_A
Cuts at: 2319 2826 2319
Size: 507 6964
PmeI GTTT'AAAC
Cuts at: 4775 4775
Size: 7471
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3498 3530 5540 5572 7053 7085 3498
Size: 32 2010 32 1481 32 3884
Fragments arranged by size:
3884 2010 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2186 2186
Size: 7471
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7471
PsiI TTA'TAA
Cuts at: 5041 5041
Size: 7471
PstI C_TGCA'G
Cuts at: 830 4823 830
Size: 3993 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7471
PvuII CAG'CTG
Cuts at: 2014 2949 2014
Size: 935 6536
SacI G_AGCT'C
Cuts at: 4680 4680
Size: 7471
SalI G'TCGA_C
Cuts at: 1936 4813 1936
Size: 2877 4594
SanDI GG'GwC_CC
Cuts at: 2186 2186
Size: 7471
SapI GCTCTTCn'nnn_
Cuts at: 2522 2522
Size: 7471
SbfI CC_TGCA'GG
Cuts at: 4823 4823
Size: 7471
ScaI AGT'ACT
Cuts at: 5719 7431 5719
Size: 1712 5759
SfcI C'TryA_G
Cuts at: 826 1045 4819 5954 6635 6826 826
Size: 219 3774 1135 681 191 1471
Fragments arranged by size:
3774 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7471
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7471
SmaI CCC'GGG
Cuts at: 1872 3474 4798 1872
Size: 1602 1324 4545
Fragments arranged by size:
4545 1602 1324
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4244 5575 6476 6738 820
Size: 197 926 2301 1331 901 262 1553
Fragments arranged by size:
2301 1553 1331 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5262 493
Size: 4769 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7471
SphI G_CATG'C
Cuts at: 1132 4829 1132
Size: 3697 3774
SspI AAT'ATT
Cuts at: 5 52 5395 5
Size: 47 5343 2081
Fragments arranged by size:
5343 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7471
StyI C'CwwG_G
Cuts at: 513 1065 2004 2181 2628 2650 4281 4910 513
Size: 552 939 177 447 22 1631 629 3074
Fragments arranged by size:
3074 1631 939 629 552 447 177 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2473 2923 4195 4688 5498 5515 5668
Size: 1255 893 450 1272 493 810 17 153
Cuts at: 5668 5853 6298 325
Size: 185 445 1498
Fragments arranged by size:
1498 1272 1255 893 810 493 450 445
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4313 4386 4539
Size: 274 80 33 51 1443 2336 73 153
Cuts at: 4539 4729 5717 7429 96
Size: 190 988 1712 138
Fragments arranged by size:
2336 1712 1443 988 274 190 153 138
80 73 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7431 7463 7431
Size: 32 7439
Tth111I GACn'n_nGTC
Cuts at: 1316 3245 1316
Size: 1929 5542
XbaI T'CTAG_A
Cuts at: 4807 4807
Size: 7471
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2302 3751 3826 3955 2302
Size: 1449 75 129 5818
Fragments arranged by size:
5818 1449 129 75
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7471
XmaI C'CCGG_G
Cuts at: 1870 3472 4796 1870
Size: 1602 1324 4545
Fragments arranged by size:
4545 1602 1324
XmnI GAAnn'nnTTC
Cuts at: 3199 5600 3199
Size: 2401 5070
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2149 5279 276
Size: 53 83 186 936 615 3130 2468
Fragments arranged by size:
3130 2468 936 615 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SanDI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI BaeI BbeI BsiWI BsmBI BstXI
DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII
SexAI SfiI SfoI SrfI SwaI