Restriction map for
pFN21AE2098
(Circular) (Six-base) MAPSORT of: pFN21AE2098.seq Check: 9020 from: 1 to: 7141
pFN21AE2098 7141 bp
With 182 enzymes: SgfI *
August 16, 2010 14:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2619 2965 3459 4311 2619
Size: 346 494 852 5449
Fragments arranged by size:
5449 852 494 346
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4951 278
Size: 53 83 186 936 3415 2468
Fragments arranged by size:
3415 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4484 1937
Size: 2547 4594
Acc65I G'GTAC_C
Cuts at: 4462 4462
Size: 7141
AclI AA'CG_TT
Cuts at: 1563 5268 5641 1563
Size: 3705 373 3063
Fragments arranged by size:
3705 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3255 3854 4136 5204 6588 6856 1632
Size: 1623 599 282 1068 1384 268 1917
Fragments arranged by size:
1917 1623 1384 1068 599 282 268
AfeI AGC'GCT
Cuts at: 4277 4277
Size: 7141
AflII C'TTAA_G
Cuts at: 820 1017 3017 820
Size: 197 2000 4944
Fragments arranged by size:
4944 2000 197
AflIII A'CryG_T
Cuts at: 1203 2369 3623 6040 7017 1203
Size: 1166 1254 2417 977 1327
Fragments arranged by size:
2417 1327 1254 1166 977
AgeI A'CCGG_T
Cuts at: 5972 5972
Size: 7141
AhdI GACnn_n'nnGTC
Cuts at: 5870 5870
Size: 7141
AleI CACnn'nnGTG
Cuts at: 1166 3820 1166
Size: 2654 4487
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7141
AlwNI CAG_nnn'CTG
Cuts at: 2777 3233 3859 4012 4884 6456 2777
Size: 456 626 153 872 1572 3462
Fragments arranged by size:
3462 1572 872 626 456 153
ApaI G_GGCC'C
Cuts at: 2123 2206 2827 3544 3726 2123
Size: 83 621 717 182 5538
Fragments arranged by size:
5538 717 621 182 83
ApaLI G'TGCA_C
Cuts at: 5198 6354 5198
Size: 1156 5985
ApoI r'AATT_y
Cuts at: 1493 4450 4676 4828 6937 1493
Size: 2957 226 152 2109 1697
Fragments arranged by size:
2957 2109 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2026 2026
Size: 7141
AseI AT'TA_AT
Cuts at: 160 1032 5695 160
Size: 872 4663 1606
Fragments arranged by size:
4663 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7141
AvaI C'yCGr_G
Cuts at: 1870 1943 2015 2085 2116 2814 2832 2964 3841
Size: 73 72 70 31 698 18 132 877
Cuts at: 3841 4466 1870
Size: 625 4545
Fragments arranged by size:
4545 877 698 625 132 73 72 70
31 18
BamHI G'GATC_C
Cuts at: 4471 4838 4471
Size: 367 6774
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2011 2095 2313 3062 3133
Size: 325 217 627 224 84 218 749 71
Cuts at: 3133 4462 5917 618
Size: 1329 1455 1842
Fragments arranged by size:
1842 1455 1329 749 627 325 224 218
217 84 71
BanII G_rGCy'C
Cuts at: 1391 2111 2123 2206 2553 2827 2861 3461 3509
Size: 720 12 83 347 274 34 600 48
Cuts at: 3509 3544 3726 3805 4460 1391
Size: 35 182 79 655 4072
Fragments arranged by size:
4072 720 655 600 347 274 182 83
79 48 35 34 12
BbeI G_GCGC'C
Cuts at: 2015 2099 2317 2015
Size: 84 218 6839
Fragments arranged by size:
6839 218 84
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2345 2427 3243 928
Size: 971 446 82 816 4826
Fragments arranged by size:
4826 971 816 446 82
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2627 2661 5332 5366 1903
Size: 34 690 34 2671 34 3678
Fragments arranged by size:
3678 2671 690 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5033 6243 5033
Size: 1210 5931
BclI T'GATC_A
Cuts at: 1548 2455 1548
Size: 907 6234
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2909 4246
Size: 107 122 71 631 605 337 900 1337
Cuts at: 4246 5752 136
Size: 1506 1525
Fragments arranged by size:
1525 1506 1337 900 631 605 337 122
107 71
BglII A'GATC_T
Cuts at: 7136 7136
Size: 7141
BlpI GC'TnA_GC
Cuts at: 2547 2634 2886 3145 3455 4558 2547
Size: 87 252 259 310 1103 5130
Fragments arranged by size:
5130 1103 310 259 252 87
Bme1580I G_kGCm'C
Cuts at: 1790 2123 2206 2827 3136 3544 3577 3726 5202
Size: 333 83 621 309 408 33 149 1476
Cuts at: 5202 6358 1790
Size: 1156 2573
Fragments arranged by size:
2573 1476 1156 621 408 333 309 149
83 33
BmgBI CAC'GTC
Cuts at: 1318 2082 2864 1318
Size: 764 782 5595
Fragments arranged by size:
5595 782 764
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4872 5830 442
Size: 416 53 83 399 24 3455 958 1753
Fragments arranged by size:
3455 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3144 1056
Size: 2088 5053
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2207 2588 3069 3494 3635 3791 4274 4292
Size: 968 381 481 425 141 156 483 18
Cuts at: 4292 5801 1239
Size: 1509 2579
Fragments arranged by size:
2579 1509 968 483 481 425 381 156
141 18
Bpu10I CC'TnA_GC
Cuts at: 3159 3159
Size: 7141
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2267 2582 5266 6131 6429 2267
Size: 315 2684 865 298 2979
Fragments arranged by size:
2979 2684 865 315 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2564 5804 882
Size: 617 1065 3240 2219
Fragments arranged by size:
3240 2219 1065 617
BsaAI yAC'GTr
Cuts at: 493 1204 2417 3626 4932 493
Size: 711 1213 1209 1306 2702
Fragments arranged by size:
2702 1306 1213 1209 711
BsaBI GATnn'nnATC
Cuts at: 7135 7135
Size: 7141
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2012 2096 2314 2446
Size: 53 83 186 936 479 84 218 132
Cuts at: 2446 4948 5330 5965 275
Size: 2502 382 635 1451
Fragments arranged by size:
2502 1451 936 635 479 382 218 186
132 84 83 53
BsaWI w'CCGG_w
Cuts at: 5574 5972 6246 6393 6804 5574
Size: 398 274 147 411 5911
Fragments arranged by size:
5911 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2066 2096 1178
Size: 30 165 30 663 30 6223
Fragments arranged by size:
6223 663 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3088 3764 4189 1186
Size: 1902 676 425 4138
Fragments arranged by size:
4138 1902 676 425
BseYI C'CCAG_C
Cuts at: 3037 3338 3804 4063 4278 6344 6840 3037
Size: 301 466 259 215 2066 496 3338
Fragments arranged by size:
3338 2066 496 466 301 259 215
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2462 2573 2937 3014 3246 1163
Size: 573 726 111 364 77 232 5058
Fragments arranged by size:
5058 726 573 364 232 111 77
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4591 5352 6380 1540
Size: 275 186 2590 761 1028 2301
Fragments arranged by size:
2590 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2111 2984 3205 3461 3643 3827 3996 5202 5287
Size: 873 221 256 182 184 169 1206 85
Cuts at: 5287 6358 2111
Size: 1071 2894
Fragments arranged by size:
2894 1206 1071 873 256 221 184 182
169 85
BsmI GAATG_Cn'
Cuts at: 3721 4156 4652 4745 3721
Size: 435 496 93 6117
Fragments arranged by size:
6117 496 435 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2111 2123 2206 2553 2827 2861 2984
Size: 399 321 12 83 347 274 34 123
Cuts at: 2984 3136 3205 3461 3509 3544 3577 3643 3726
Size: 152 69 256 48 35 33 66 83
Cuts at: 3726 3805 3827 3996 4460 5202 5287 6358 1391
Size: 79 22 169 464 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 464 399 347 321 274
256 169 152 123 85 83 83 79
69 66 48 35 34 33 22 12
BspEI T'CCGG_A
Cuts at: 6804 6804
Size: 7141
BspHI T'CATG_A
Cuts at: 5030 6760 5030
Size: 1730 5411
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2619 2965 3459 4311 4385 4496 844
Size: 1775 346 494 852 74 111 3489
Fragments arranged by size:
3489 1775 852 494 346 111 74
BsrBI CCG'CTC
Cuts at: 1394 1823 2193 5028 1394
Size: 429 370 2835 3507
Fragments arranged by size:
3507 2835 429 370
BsrDI GCAATG_nn'
Cuts at: 65 1248 5636 5810 65
Size: 1183 4388 174 1396
Fragments arranged by size:
4388 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2916 5785 5972 1700
Size: 110 1106 2869 187 2869
Fragments arranged by size:
2869 2869 1106 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7141
BssHII G'CGCG_C
Cuts at: 1925 2026 1925
Size: 101 7040
BssSI C'ACGA_G
Cuts at: 5201 6213 5201
Size: 1012 6129
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2656 794
Size: 1862 5279
BstBI TT'CG_AA
Cuts at: 5945 5945
Size: 7141
BstEII G'GTnAC_C
Cuts at: 1184 3072 4298 1184
Size: 1888 1226 4027
Fragments arranged by size:
4027 1888 1226
BstXI CCAn_nnnn'nTGG
Cuts at: 2389 3346 3899 4270 2389
Size: 957 553 371 5260
Fragments arranged by size:
5260 957 553 371
BstYI r'GATC_y
Cuts at: 1972 3334 4471 4838 5223 5240 6692 6778 6790
Size: 1362 1137 367 385 17 1452 86 12
Cuts at: 6790 7136 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1362 1137 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2070 2674 3794 4285 4437 513
Size: 552 939 66 604 1120 491 152 3217
Fragments arranged by size:
3217 1120 939 604 552 491 152 66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2905 3759 487
Size: 683 56 1679 854 3869
Fragments arranged by size:
3869 1679 854 683 56
BtsI GCAGTG_nn'
Cuts at: 3602 3641 3815 4657 5451 5471 3602
Size: 39 174 842 794 20 5272
Fragments arranged by size:
5272 842 794 174 39 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7141
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7106
DraI TTT'AAA
Cuts at: 4445 4445
Size: 7141
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6148 809
Size: 507 4832 1802
Fragments arranged by size:
4832 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2577 2910 4588
Size: 54 1425 50 275 18 747 333 1678
Cuts at: 4588 5477 7106 8
Size: 889 1629 43
Fragments arranged by size:
1678 1629 1425 889 747 333 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4588 1537
Size: 275 2776 4090
Fragments arranged by size:
4090 2776 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2099 2478 3112 3282 4316 5071 1353
Size: 746 379 634 170 1034 755 3423
Fragments arranged by size:
3423 1034 755 746 634 379 170
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2594 3021 3509 5728 6102 6248
Size: 1064 138 1005 427 488 2219 374 146
Cuts at: 6248 387
Size: 1280
Fragments arranged by size:
2219 1280 1064 1005 488 427 374 146
138
EcoICRI GAG'CTC
Cuts at: 2109 3459 2109
Size: 1350 5791
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2207 2588 3069 3255 3494 3635 3791
Size: 393 575 381 481 186 239 141 156
Cuts at: 3791 3854 4136 4274 4292 5204 5801 6588 6856
Size: 63 282 138 18 912 597 787 268
Cuts at: 6856 1239
Size: 1524
Fragments arranged by size:
1524 912 787 597 575 481 393 381
282 268 239 186 156 141 138 63
18
EcoO109I rG'GnC_Cy
Cuts at: 2120 2203 2774 2824 2844 3218 3541 3658 3722
Size: 83 571 50 20 374 323 117 64
Cuts at: 3722 3723 4120 2120
Size: 1 397 5141
Fragments arranged by size:
5141 571 397 374 323 117 83 64
50 20 1
EcoRI G'AATT_C
Cuts at: 4450 4828 4450
Size: 378 6763
FspI TGC'GCA
Cuts at: 5647 5647
Size: 7141
HaeII r_GCGC'y
Cuts at: 1678 2015 2099 2172 2317 4063 4279 6288 1678
Size: 337 84 73 145 1746 216 2009 2531
Fragments arranged by size:
2531 2009 1746 337 216 145 84 73
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2066 2098 2828 2860 3552
Size: 32 135 32 494 32 730 32 692
Cuts at: 3552 3581 3584 3613 4166 4178 4181 4198 4210
Size: 29 3 29 553 12 3 17 12
Cuts at: 4210 4213 5782 5814 5856 5888 1373
Size: 3 1569 32 42 32 2626
Fragments arranged by size:
2626 1569 730 692 553 494 135 42
32 32 32 32 32 32 29 29
17 12 12 3 3 3
HincII GTy'rAC
Cuts at: 669 1657 1938 3676 4485 4731 669
Size: 988 281 1738 809 246 3079
Fragments arranged by size:
3079 1738 988 809 281 246
HindIII A'AGCT_T
Cuts at: 748 3366 748
Size: 2618 4523
HpaI GTT'AAC
Cuts at: 4731 4731
Size: 7141
KasI G'GCGC_C
Cuts at: 2011 2095 2313 2011
Size: 84 218 6839
Fragments arranged by size:
6839 218 84
KpnI G_GTAC'C
Cuts at: 4466 4466
Size: 7141
MfeI C'AATT_G
Cuts at: 4740 4740
Size: 7141
MluI A'CGCG_T
Cuts at: 7017 7017
Size: 7141
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2521 3759 4251 6255 6439 1305
Size: 1216 1238 492 2004 184 2007
Fragments arranged by size:
2007 2004 1238 1216 492 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2579 2912 10
Size: 54 1425 343 747 333 4239
Fragments arranged by size:
4239 1425 747 343 333 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2429 3585 3820 5099 5458 5617 518
Size: 648 1263 1156 235 1279 359 159 2042
Fragments arranged by size:
2042 1279 1263 1156 648 359 235 159
MspA1I CmG'CkG
Cuts at: 1606 2461 2653 3342 3691 3859 4213 4557 5234
Size: 855 192 689 349 168 354 344 677
Cuts at: 5234 6382 6627 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 855 689 677 354 349 344
245 192 168
NaeI GCC'GGC
Cuts at: 2918 2918
Size: 7141
NarI GG'CG_CC
Cuts at: 2012 2096 2314 2012
Size: 84 218 6839
Fragments arranged by size:
6839 218 84
NcoI C'CATG_G
Cuts at: 513 1065 2004 2070 2674 4437 513
Size: 552 939 66 604 1763 3217
Fragments arranged by size:
3217 1763 939 604 552 66
NdeI CA'TA_TG
Cuts at: 387 2175 387
Size: 1788 5353
NgoMIV G'CCGG_C
Cuts at: 2916 2916
Size: 7141
NheI G'CTAG_C
Cuts at: 1052 3140 1052
Size: 2088 5053
NotI GC'GGCC_GC
Cuts at: 4588 4588
Size: 7141
NspI r_CATG'y
Cuts at: 1132 1229 2373 2521 4499 6044 1132
Size: 97 1144 148 1978 1545 2229
Fragments arranged by size:
2229 1978 1545 1144 148 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7141
PciI A'CATG_T
Cuts at: 2369 6040 2369
Size: 3671 3470
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2777 1108
Size: 1669 5472
PfoI T'CCnGG_A
Cuts at: 3612 3612
Size: 7141
PmeI GTTT'AAAC
Cuts at: 4445 4445
Size: 7141
PmlI CAC'GTG
Cuts at: 3626 3626
Size: 7141
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5210 5242 6723 6755 5210
Size: 32 1481 32 5596
Fragments arranged by size:
5596 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2844 3658 2844
Size: 814 6327
PshAI GACnn'nnGTC
Cuts at: 1613 2445 1613
Size: 832 6309
PsiI TTA'TAA
Cuts at: 4711 4711
Size: 7141
PspOMI G'GGCC_C
Cuts at: 2119 2202 2823 3540 3722 2119
Size: 83 621 717 182 5538
Fragments arranged by size:
5538 717 621 182 83
PstI C_TGCA'G
Cuts at: 830 2526 2902 3190 3855 4493 830
Size: 1696 376 288 665 638 3478
Fragments arranged by size:
3478 1696 665 638 376 288
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7141
PvuII CAG'CTG
Cuts at: 2653 3342 3859 4213 2653
Size: 689 517 354 5581
Fragments arranged by size:
5581 689 517 354
SacI G_AGCT'C
Cuts at: 2111 3461 2111
Size: 1350 5791
SalI G'TCGA_C
Cuts at: 1936 4483 1936
Size: 2547 4594
SanDI GG'GwC_CC
Cuts at: 2844 2844
Size: 7141
SapI GCTCTTCn'nnn_
Cuts at: 2099 2099
Size: 7141
SbfI CC_TGCA'GG
Cuts at: 4493 4493
Size: 7141
ScaI AGT'ACT
Cuts at: 5389 7101 5389
Size: 1712 5429
SfcI C'TryA_G
Cuts at: 826 1045 2522 2898 3186 3851 4004 4489 5624
Size: 219 1477 376 288 665 153 485 1135
Cuts at: 5624 6305 6496 826
Size: 681 191 1471
Fragments arranged by size:
1477 1471 1135 681 665 485 376 288
219 191 153
SfoI GGC'GCC
Cuts at: 2013 2097 2315 2013
Size: 84 218 6839
Fragments arranged by size:
6839 218 84
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7141
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7141
SmaI CCC'GGG
Cuts at: 1872 2087 4468 1872
Size: 215 2381 4545
Fragments arranged by size:
4545 2381 215
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2282 2597 3017 5245 6146 6408
Size: 197 926 339 315 420 2228 901 262
Cuts at: 6408 820
Size: 1553
Fragments arranged by size:
2228 1553 926 901 420 339 315 262
197
SnaBI TAC'GTA
Cuts at: 493 4932 493
Size: 4439 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7141
SphI G_CATG'C
Cuts at: 1132 4499 1132
Size: 3367 3774
SspI AAT'ATT
Cuts at: 5 52 5065 5
Size: 47 5013 2081
Fragments arranged by size:
5013 2081 47
StuI AGG'CCT
Cuts at: 1517 2021 2292 2347 2396 2810 3839 4415 1517
Size: 504 271 55 49 414 1029 576 4243
Fragments arranged by size:
4243 1029 576 504 414 271 55 49
StyI C'CwwG_G
Cuts at: 513 1065 2004 2070 2674 3396 4167 4437 4580
Size: 552 939 66 604 722 771 270 143
Cuts at: 4580 513
Size: 3074
Fragments arranged by size:
3074 939 771 722 604 552 270 143
66
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3454 3696 5168 5185 5338 5523 5968
Size: 1255 1874 242 1472 17 153 185 445
Cuts at: 5968 325
Size: 1498
Fragments arranged by size:
1874 1498 1472 1255 445 242 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3927 5387 7099
Size: 274 80 33 51 1443 1950 1460 1712
Cuts at: 7099 96
Size: 138
Fragments arranged by size:
1950 1712 1460 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3184 3216 3363 3395 3594 3626 7101 7133 3184
Size: 32 147 32 199 32 3475 32 3192
Fragments arranged by size:
3475 3192 199 147 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7141
XbaI T'CTAG_A
Cuts at: 4477 4477
Size: 7141
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2414 3056 2414
Size: 642 6499
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7141
XmaI C'CCGG_G
Cuts at: 1870 2085 4466 1870
Size: 215 2381 4545
Fragments arranged by size:
4545 2381 215
XmnI GAAnn'nnTTC
Cuts at: 5270 5270
Size: 7141
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4949 276
Size: 53 83 186 936 3415 2468
Fragments arranged by size:
3415 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI
NruI NsiI PacI PspXI PsrI RsrII SacII SexAI
SfiI SrfI SwaI