Restriction map for
pFN21AE2090
(Circular) (Six-base) MAPSORT of: pFN21AE2090.seq Check: 1846 from: 1 to: 7012
pFN21AE2090 7012 bp
With 182 enzymes: SgfI *
July 6, 2012 17:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4243 4243
Size: 7012
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4822 278
Size: 53 83 186 936 3286 2468
Fragments arranged by size:
3286 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4355 1937
Size: 2418 4594
Acc65I G'GTAC_C
Cuts at: 4333 4333
Size: 7012
AclI AA'CG_TT
Cuts at: 1563 5139 5512 1563
Size: 3576 373 3063
Fragments arranged by size:
3576 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2172 2598 3716 4005 5075 6459 6727 1632
Size: 540 426 1118 289 1070 1384 268 1917
Fragments arranged by size:
1917 1384 1118 1070 540 426 289 268
AflII C'TTAA_G
Cuts at: 820 1017 4247 820
Size: 197 3230 3585
Fragments arranged by size:
3585 3230 197
AflIII A'CryG_T
Cuts at: 1203 3186 5911 6888 1203
Size: 1983 2725 977 1327
Fragments arranged by size:
2725 1983 1327 977
AgeI A'CCGG_T
Cuts at: 3986 5843 3986
Size: 1857 5155
AhdI GACnn_n'nnGTC
Cuts at: 5741 5741
Size: 7012
AleI CACnn'nnGTG
Cuts at: 1166 3571 1166
Size: 2405 4607
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2469 1184
Size: 1285 5727
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2214 2246 3585 3617 2214
Size: 32 1339 32 5609
Fragments arranged by size:
5609 1339 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2522 4258 4755 6327 2522
Size: 1736 497 1572 3207
Fragments arranged by size:
3207 1736 1572 497
ApaLI G'TGCA_C
Cuts at: 5069 6225 5069
Size: 1156 5856
ApoI r'AATT_y
Cuts at: 1493 2050 2383 2805 2980 3151 3710 3762 3877
Size: 557 333 422 175 171 559 52 115
Cuts at: 3877 4129 4321 4547 4699 6808 1493
Size: 252 192 226 152 2109 1697
Fragments arranged by size:
2109 1697 559 557 422 333 252 226
192 175 171 152 115 52
AseI AT'TA_AT
Cuts at: 160 1032 5566 160
Size: 872 4534 1606
Fragments arranged by size:
4534 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7012
AvaI C'yCGr_G
Cuts at: 1870 1943 4337 1870
Size: 73 2394 4545
Fragments arranged by size:
4545 2394 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3159 3192 3159
Size: 33 6979
BamHI G'GATC_C
Cuts at: 4342 4709 4342
Size: 367 6645
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3635 4333 5788 618
Size: 325 217 627 1848 698 1455 1842
Fragments arranged by size:
1848 1842 1455 698 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4331 1391
Size: 2940 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2020 2148 2504 2922 4249 928
Size: 971 121 128 356 418 1327 3691
Fragments arranged by size:
3691 1327 971 418 356 128 121
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5203 5237 1903
Size: 34 3266 34 3678
Fragments arranged by size:
3678 3266 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4904 6114 4904
Size: 1210 5802
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7012
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2548 5623 136
Size: 107 122 71 631 605 876 3075 1525
Fragments arranged by size:
3075 1525 876 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7007 7007
Size: 7012
BlpI GC'TnA_GC
Cuts at: 4429 4429
Size: 7012
Bme1580I G_kGCm'C
Cuts at: 1790 3640 5073 6229 1790
Size: 1850 1433 1156 2573
Fragments arranged by size:
2573 1850 1433 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7012
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4187 4743 5701
Size: 416 53 83 399 24 2770 556 958
Cuts at: 5701 442
Size: 1753
Fragments arranged by size:
2770 1753 958 556 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7012
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2517 2850 3156 3500 3804 5672 1239
Size: 1278 333 306 344 304 1868 2579
Fragments arranged by size:
2579 1868 1278 344 333 306 304
Bpu10I CC'TnA_GC
Cuts at: 4001 4001
Size: 7012
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2841 3651 5137 6002 6300 2841
Size: 810 1486 865 298 3553
Fragments arranged by size:
3553 1486 865 810 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3579 5675 882
Size: 617 2080 2096 2219
Fragments arranged by size:
2219 2096 2080 617
BsaAI yAC'GTr
Cuts at: 493 1204 4803 493
Size: 711 3599 2702
Fragments arranged by size:
3599 2702 711
BsaBI GATnn'nnATC
Cuts at: 7006 7006
Size: 7012
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4819 5201 5836 275
Size: 53 83 186 936 3286 382 635 1451
Fragments arranged by size:
3286 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3986 4204 5445 5843 6117 6264 6675 3986
Size: 218 1241 398 274 147 411 4323
Fragments arranged by size:
4323 1241 411 398 274 218 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6787
Fragments arranged by size:
6787 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7012
BseYI C'CCAG_C
Cuts at: 3300 6215 6711 3300
Size: 2915 496 3601
Fragments arranged by size:
3601 2915 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2639 1163
Size: 573 903 5536
Fragments arranged by size:
5536 903 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4462 5223 6251 1540
Size: 275 186 2461 761 1028 2301
Fragments arranged by size:
2461 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5073 5158 6229 5073
Size: 85 1071 5856
Fragments arranged by size:
5856 1071 85
BsmI GAATG_Cn'
Cuts at: 2851 3952 4523 4616 2851
Size: 1101 571 93 5247
Fragments arranged by size:
5247 1101 571 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3640 4331 5073 5158 6229 1391
Size: 399 1850 691 742 85 1071 2174
Fragments arranged by size:
2174 1850 1071 742 691 399 85
BspEI T'CCGG_A
Cuts at: 6675 6675
Size: 7012
BspHI T'CATG_A
Cuts at: 4901 6631 4901
Size: 1730 5282
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4243 4367 844
Size: 3399 124 3489
Fragments arranged by size:
3489 3399 124
BsrBI CCG'CTC
Cuts at: 1394 1823 4899 1394
Size: 429 3076 3507
Fragments arranged by size:
3507 3076 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2603 5507 5681 65
Size: 1183 1355 2904 174 1396
Fragments arranged by size:
2904 1396 1355 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3986 5656 5843 1700
Size: 110 2176 1670 187 2869
Fragments arranged by size:
2869 2176 1670 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7012
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7012
BssSI C'ACGA_G
Cuts at: 4048 5072 6084 4048
Size: 1024 1012 4976
Fragments arranged by size:
4976 1024 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7012
BstBI TT'CG_AA
Cuts at: 5816 5816
Size: 7012
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7012
BstXI CCAn_nnnn'nTGG
Cuts at: 2874 2874
Size: 7012
BstYI r'GATC_y
Cuts at: 1972 3600 4342 4709 5094 5111 6563 6649 6661
Size: 1628 742 367 385 17 1452 86 12
Cuts at: 6661 7007 1972
Size: 346 1977
Fragments arranged by size:
1977 1628 1452 742 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2656 2656
Size: 7012
BtgI C'CryG_G
Cuts at: 513 1065 3312 513
Size: 552 2247 4213
Fragments arranged by size:
4213 2247 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6273
Fragments arranged by size:
6273 683 56
BtsI GCAGTG_nn'
Cuts at: 4528 5322 5342 4528
Size: 794 20 6198
Fragments arranged by size:
6198 794 20
ClaI AT'CG_AT
Cuts at: 1554 3024 1554
Size: 1470 5542
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2922 2957 529
Size: 35 2358 35 4584
Fragments arranged by size:
4584 2358 35 35
DraI TTT'AAA
Cuts at: 2958 3715 4316 2958
Size: 757 601 5654
Fragments arranged by size:
5654 757 601
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2527 6019 809
Size: 507 1211 3492 1802
Fragments arranged by size:
3492 1802 1211 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3315 4459 5348
Size: 54 1425 50 275 18 1485 1144 889
Cuts at: 5348 6977 8
Size: 1629 43
Fragments arranged by size:
1629 1485 1425 1144 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4459 1537
Size: 275 2647 4090
Fragments arranged by size:
4090 2647 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2269 3692 3926 4942 1353
Size: 916 1423 234 1016 3423
Fragments arranged by size:
3423 1423 1016 916 234
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5599 5973 6119 387
Size: 1064 138 4010 374 146 1280
Fragments arranged by size:
4010 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2172 2517 2598 2850 3156 3500 3716
Size: 393 540 345 81 252 306 344 216
Cuts at: 3716 3804 4005 5075 5672 6459 6727 1239
Size: 88 201 1070 597 787 268 1524
Fragments arranged by size:
1524 1070 787 597 540 393 345 344
306 268 252 216 201 88 81
EcoNI CCTnn'n_nnAGG
Cuts at: 3734 3734
Size: 7012
EcoRI G'AATT_C
Cuts at: 3151 3762 4129 4321 4699 3151
Size: 611 367 192 378 5464
Fragments arranged by size:
5464 611 378 367 192
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3282 4295 3282
Size: 1013 5999
FspI TGC'GCA
Cuts at: 5518 5518
Size: 7012
HaeII r_GCGC'y
Cuts at: 1678 6159 1678
Size: 4481 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3193 3225 3269 3301 3338
Size: 32 135 32 1621 32 44 32 37
Cuts at: 3338 3370 3602 3634 5653 5685 5727 5759 1373
Size: 32 232 32 2019 32 42 32 2626
Fragments arranged by size:
2626 2019 1621 232 135 44 42 37
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4356 4602 669
Size: 988 281 2418 246 3079
Fragments arranged by size:
3079 2418 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7012
HpaI GTT'AAC
Cuts at: 4602 4602
Size: 7012
KpnI G_GTAC'C
Cuts at: 4337 4337
Size: 7012
MfeI C'AATT_G
Cuts at: 2285 2834 4611 2285
Size: 549 1777 4686
Fragments arranged by size:
4686 1777 549
MluI A'CGCG_T
Cuts at: 6888 6888
Size: 7012
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3424 6126 6310 1305
Size: 2119 2702 184 2007
Fragments arranged by size:
2702 2119 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3317 10
Size: 54 1425 343 1485 3705
Fragments arranged by size:
3705 1485 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3571 4970 5329 5488 518
Size: 648 2405 1399 359 159 2042
Fragments arranged by size:
2405 2042 1399 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4428 5105 6253 6498 1606
Size: 2822 677 1148 245 2120
Fragments arranged by size:
2822 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 3312 513
Size: 552 2247 4213
Fragments arranged by size:
4213 2247 552
NdeI CA'TA_TG
Cuts at: 387 3378 387
Size: 2991 4021
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7012
NotI GC'GGCC_GC
Cuts at: 4459 4459
Size: 7012
NspI r_CATG'y
Cuts at: 1132 1229 2089 4370 5915 1132
Size: 97 860 2281 1545 2229
Fragments arranged by size:
2281 2229 1545 860 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7012
PciI A'CATG_T
Cuts at: 5911 5911
Size: 7012
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7012
PmeI GTTT'AAAC
Cuts at: 4316 4316
Size: 7012
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5081 5113 6594 6626 5081
Size: 32 1481 32 5467
Fragments arranged by size:
5467 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7012
PsiI TTA'TAA
Cuts at: 3954 4582 3954
Size: 628 6384
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2326 2358 2326
Size: 32 6980
PstI C_TGCA'G
Cuts at: 830 4364 830
Size: 3534 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7012
SalI G'TCGA_C
Cuts at: 1936 4354 1936
Size: 2418 4594
SapI GCTCTTCn'nnn_
Cuts at: 2269 2269
Size: 7012
SbfI CC_TGCA'GG
Cuts at: 4364 4364
Size: 7012
ScaI AGT'ACT
Cuts at: 5260 6972 5260
Size: 1712 5300
SfcI C'TryA_G
Cuts at: 826 1045 2823 2894 4360 5495 6176 6367 826
Size: 219 1778 71 1466 1135 681 191 1471
Fragments arranged by size:
1778 1471 1466 1135 681 219 191 71
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7012
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7012
SmaI CCC'GGG
Cuts at: 1872 4339 1872
Size: 2467 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2856 3666 4247 5116 6017 6279
Size: 197 926 913 810 581 869 901 262
Cuts at: 6279 820
Size: 1553
Fragments arranged by size:
1553 926 913 901 869 810 581 262
197
SnaBI TAC'GTA
Cuts at: 493 4803 493
Size: 4310 2702
SpeI A'CTAG_T
Cuts at: 152 2176 152
Size: 2024 4988
SphI G_CATG'C
Cuts at: 1132 4370 1132
Size: 3238 3774
SspI AAT'ATT
Cuts at: 5 52 3244 4936 5
Size: 47 3192 1692 2081
Fragments arranged by size:
3192 2081 1692 47
StuI AGG'CCT
Cuts at: 1517 2672 1517
Size: 1155 5857
StyI C'CwwG_G
Cuts at: 513 1065 3312 3640 4451 513
Size: 552 2247 328 811 3074
Fragments arranged by size:
3074 2247 811 552 328
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5039 5056 5209 5394 5839 325
Size: 1255 3459 17 153 185 445 1498
Fragments arranged by size:
3459 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3452 5258 6970
Size: 274 80 33 51 1443 1475 1806 1712
Cuts at: 6970 96
Size: 138
Fragments arranged by size:
1806 1712 1475 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2791 2823 6972 7004 2791
Size: 32 4149 32 2799
Fragments arranged by size:
4149 2799 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7012
XbaI T'CTAG_A
Cuts at: 4348 4348
Size: 7012
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3779 3779
Size: 7012
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7012
XmaI C'CCGG_G
Cuts at: 1870 4337 1870
Size: 2467 4545
XmnI GAAnn'nnTTC
Cuts at: 2386 4221 5141 2386
Size: 1835 920 4257
Fragments arranged by size:
4257 1835 920
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4820 276
Size: 53 83 186 936 3286 2468
Fragments arranged by size:
3286 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PsrI PstI PvuI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BbeI BbvCI BfrBI BplI
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoO109I EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PvuII RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI