Restriction map for
pFN21AE2089
(Circular) (Six-base) MAPSORT of: pFN21AE2089.seq Check: 7213 from: 1 to: 6997
pFN21AE2089 6997 bp
With 182 enzymes: SgfI *
August 16, 2010 14:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2033 2033
Size: 6997
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4807 278
Size: 53 83 186 936 3271 2468
Fragments arranged by size:
3271 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2421 4011 4340 1937
Size: 484 1590 329 4594
Fragments arranged by size:
4594 1590 484 329
Acc65I G'GTAC_C
Cuts at: 3400 4318 3400
Size: 918 6079
AclI AA'CG_TT
Cuts at: 1563 5124 5497 1563
Size: 3561 373 3063
Fragments arranged by size:
3561 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 5060 6444 6712 1632
Size: 3428 1384 268 1917
Fragments arranged by size:
3428 1917 1384 268
AfeI AGC'GCT
Cuts at: 3921 3921
Size: 6997
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6800
AflIII A'CryG_T
Cuts at: 1203 5896 6873 1203
Size: 4693 977 1327
Fragments arranged by size:
4693 1327 977
AgeI A'CCGG_T
Cuts at: 5828 5828
Size: 6997
AhdI GACnn_n'nnGTC
Cuts at: 5726 5726
Size: 6997
AleI CACnn'nnGTG
Cuts at: 1166 2282 3077 3419 3935 1166
Size: 1116 795 342 516 4228
Fragments arranged by size:
4228 1116 795 516 342
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2912 1184
Size: 1728 5269
AlwNI CAG_nnn'CTG
Cuts at: 2608 3520 3743 4740 6312 2608
Size: 912 223 997 1572 3293
Fragments arranged by size:
3293 1572 997 912 223
ApaI G_GGCC'C
Cuts at: 2786 2875 3175 2786
Size: 89 300 6608
Fragments arranged by size:
6608 300 89
ApaLI G'TGCA_C
Cuts at: 5054 6210 5054
Size: 1156 5841
ApoI r'AATT_y
Cuts at: 1493 2807 3464 4076 4306 4532 4684 6793 1493
Size: 1314 657 612 230 226 152 2109 1697
Fragments arranged by size:
2109 1697 1314 657 612 230 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5551 160
Size: 872 4519 1606
Fragments arranged by size:
4519 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6997
AvaI C'yCGr_G
Cuts at: 1870 1943 2078 2723 2729 4322 1870
Size: 73 135 645 6 1593 4545
Fragments arranged by size:
4545 1593 645 135 73 6
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3619 3652 3619
Size: 33 6964
BamHI G'GATC_C
Cuts at: 2892 3805 4327 4694 2892
Size: 913 522 367 5195
Fragments arranged by size:
5195 913 522 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2169 3269 3400 3937 4318
Size: 325 217 627 382 1100 131 537 381
Cuts at: 4318 5773 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1100 627 537 382 381 325
217 131
BanII G_rGCy'C
Cuts at: 1391 2403 2730 2759 2786 2875 2986 3175 3994
Size: 1012 327 29 27 89 111 189 819
Cuts at: 3994 4316 1391
Size: 322 4072
Fragments arranged by size:
4072 1012 819 327 322 189 111 89
29 27
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2032 2751 928
Size: 971 133 719 5174
Fragments arranged by size:
5174 971 719 133
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3535 3569 5188 5222 1903
Size: 34 50 34 1514 34 1619 34 3678
Fragments arranged by size:
3678 1619 1514 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4889 6099 4889
Size: 1210 5787
BclI T'GATC_A
Cuts at: 1548 2937 1548
Size: 1389 5608
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4226 4271 5608
Size: 107 122 71 631 605 2554 45 1337
Cuts at: 5608 136
Size: 1525
Fragments arranged by size:
2554 1525 1337 631 605 122 107 71
45
BglII A'GATC_T
Cuts at: 6992 6992
Size: 6997
BlpI GC'TnA_GC
Cuts at: 2303 2639 4414 2303
Size: 336 1775 4886
Fragments arranged by size:
4886 1775 336
Bme1580I G_kGCm'C
Cuts at: 1790 2779 2786 2875 3175 3942 5058 6214 1790
Size: 989 7 89 300 767 1116 1156 2573
Fragments arranged by size:
2573 1156 1116 989 767 300 89 7
BmgBI CAC'GTC
Cuts at: 1318 2845 1318
Size: 1527 5470
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3220 4728 5686
Size: 416 53 83 399 24 1803 1508 958
Cuts at: 5686 442
Size: 1753
Fragments arranged by size:
1803 1753 1508 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6997
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3452 3452
Size: 6997
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2212 2399 2524 3272 3370 3898 4054 4232
Size: 973 187 125 748 98 528 156 178
Cuts at: 4232 5657 1239
Size: 1425 2579
Fragments arranged by size:
2579 1425 973 748 528 187 178 156
125 98
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3380 3422 5122 5987 6285 3380
Size: 42 1700 865 298 4092
Fragments arranged by size:
4092 1700 865 298 42
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3931 4169 5660 882
Size: 617 2432 238 1491 2219
Fragments arranged by size:
2432 2219 1491 617 238
BsaAI yAC'GTr
Cuts at: 493 1204 2121 4788 493
Size: 711 917 2667 2702
Fragments arranged by size:
2702 2667 917 711
BsaBI GATnn'nnATC
Cuts at: 6991 6991
Size: 6997
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3309 4804 5186 5821
Size: 53 83 186 936 1776 1495 382 635
Cuts at: 5821 275
Size: 1451
Fragments arranged by size:
1776 1495 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5430 5828 6102 6249 6660 5430
Size: 398 274 147 411 5767
Fragments arranged by size:
5767 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3087 3117 1178
Size: 30 165 30 1684 30 5058
Fragments arranged by size:
5058 1684 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2096 2200 3062 3269 3460 1186
Size: 910 104 862 207 191 4723
Fragments arranged by size:
4723 910 862 207 191 104
BseYI C'CCAG_C
Cuts at: 2044 2298 2648 2669 2875 3424 3524 3542 6200
Size: 254 350 21 206 549 100 18 2658
Cuts at: 6200 6696 2044
Size: 496 2345
Fragments arranged by size:
2658 2345 549 496 350 254 206 100
21 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6424
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4015 4447 5208 6236 1540
Size: 275 186 2014 432 761 1028 2301
Fragments arranged by size:
2301 2014 1028 761 432 275 186
BsiHKAI G_wGCw'C
Cuts at: 2022 3114 4169 5058 5143 6214 2022
Size: 1092 1055 889 85 1071 2805
Fragments arranged by size:
2805 1092 1071 1055 889 85
BsiWI C'GTAC_G
Cuts at: 3474 3474
Size: 6997
BsmI GAATG_Cn'
Cuts at: 2482 2704 4508 4601 2482
Size: 222 1804 93 4878
Fragments arranged by size:
4878 1804 222 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2230 2230
Size: 6997
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2022 2403 2730 2759 2779 2786 2875
Size: 399 232 381 327 29 20 7 89
Cuts at: 2875 2986 3114 3175 3942 3994 4169 4316 5058
Size: 111 128 61 767 52 175 147 742
Cuts at: 5058 5143 6214 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 767 742 399 381 327 232
175 147 128 111 89 85 61 52
29 20 7
BspEI T'CCGG_A
Cuts at: 6660 6660
Size: 6997
BspHI T'CATG_A
Cuts at: 4886 6616 4886
Size: 1730 5267
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2033 4352 844
Size: 1189 2319 3489
Fragments arranged by size:
3489 2319 1189
BsrBI CCG'CTC
Cuts at: 1394 1823 4884 1394
Size: 429 3061 3507
Fragments arranged by size:
3507 3061 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2292 5492 5666 65
Size: 1183 1044 3200 174 1396
Fragments arranged by size:
3200 1396 1183 1044 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2148 2172 3311 3576 5641 5828 1700
Size: 110 338 24 1139 265 2065 187 2869
Fragments arranged by size:
2869 2065 1139 338 265 187 110 24
BsrGI T'GTAC_A
Cuts at: 96 2499 96
Size: 2403 4594
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6997
BssSI C'ACGA_G
Cuts at: 5057 6069 5057
Size: 1012 5985
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3743 794
Size: 2949 4048
BstBI TT'CG_AA
Cuts at: 5801 5801
Size: 6997
BstEII G'GTnAC_C
Cuts at: 1184 2446 3604 1184
Size: 1262 1158 4577
Fragments arranged by size:
4577 1262 1158
BstXI CCAn_nnnn'nTGG
Cuts at: 2396 2993 3659 3922 4261 2396
Size: 597 666 263 339 5132
Fragments arranged by size:
5132 666 597 339 263
BstYI r'GATC_y
Cuts at: 1972 2892 3805 4327 4694 5079 5096 6548 6634
Size: 920 913 522 367 385 17 1452 86
Cuts at: 6634 6646 6992 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 920 913 522 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2750 2787 2948 2750
Size: 37 161 6799
Fragments arranged by size:
6799 161 37
BtgI C'CryG_G
Cuts at: 513 1065 3360 3969 4260 513
Size: 552 2295 609 291 3250
Fragments arranged by size:
3250 2295 609 552 291
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2658 3732 487
Size: 683 56 1432 1074 3752
Fragments arranged by size:
3752 1432 1074 683 56
BtsI GCAGTG_nn'
Cuts at: 2833 3966 4513 5307 5327 2833
Size: 1133 547 794 20 4503
Fragments arranged by size:
4503 1133 794 547 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6997
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6962
DraI TTT'AAA
Cuts at: 4301 4301
Size: 6997
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2417 2849 6004 809
Size: 507 1101 432 3155 1802
Fragments arranged by size:
3155 1802 1101 507 432
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2599 2802 3105
Size: 54 1425 50 275 18 769 203 303
Cuts at: 3105 3181 3984 4263 4444 5333 6962 8
Size: 76 803 279 181 889 1629 43
Fragments arranged by size:
1629 1425 889 803 769 303 279 275
203 181 76 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4444 1537
Size: 275 2632 4090
Fragments arranged by size:
4090 2632 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2018 2054 2861 3041 3687 4111 4137 4927
Size: 665 36 807 180 646 424 26 790
Cuts at: 4927 1353
Size: 3423
Fragments arranged by size:
3423 807 790 665 646 424 180 36
26
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5584 5958 6104 387
Size: 1064 138 3995 374 146 1280
Fragments arranged by size:
3995 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2212 2399 2524 3272 3370 3898 4054
Size: 393 580 187 125 748 98 528 156
Cuts at: 4054 4232 5060 5657 6444 6712 1239
Size: 178 828 597 787 268 1524
Fragments arranged by size:
1524 828 787 748 597 580 528 393
268 187 178 156 125 98
EcoNI CCTnn'n_nnAGG
Cuts at: 2951 3251 3731 2951
Size: 300 480 6217
Fragments arranged by size:
6217 480 300
EcoO109I rG'GnC_Cy
Cuts at: 2393 2783 2871 2872 3171 3442 3517 3539 3711
Size: 390 88 1 299 271 75 22 172
Cuts at: 3711 2393
Size: 5679
Fragments arranged by size:
5679 390 299 271 172 88 75 22
1
EcoRI G'AATT_C
Cuts at: 4306 4684 4306
Size: 378 6619
FspI TGC'GCA
Cuts at: 5503 5503
Size: 6997
HaeII r_GCGC'y
Cuts at: 1678 2326 2971 3923 6144 1678
Size: 648 645 952 2221 2531
Fragments arranged by size:
2531 2221 952 648 645
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3909 3941 4008 4040 5638
Size: 32 135 32 2337 32 67 32 1598
Cuts at: 5638 5670 5712 5744 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2337 1598 135 67 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2146 2854 4012 4341 4587 669
Size: 988 281 208 708 1158 329 246 3079
Fragments arranged by size:
3079 1158 988 708 329 281 246 208
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6997
HpaI GTT'AAC
Cuts at: 4587 4587
Size: 6997
KpnI G_GTAC'C
Cuts at: 3404 4322 3404
Size: 918 6079
MfeI C'AATT_G
Cuts at: 4596 4596
Size: 6997
MluI A'CGCG_T
Cuts at: 6873 6873
Size: 6997
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6111 6295 1305
Size: 4806 184 2007
Fragments arranged by size:
4806 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2601 2804 3986 10
Size: 54 1425 343 769 203 1182 3021
Fragments arranged by size:
3021 1425 1182 769 343 203 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2282 2991 3077 3419 3657 3935 4049
Size: 648 1116 709 86 342 238 278 114
Cuts at: 4049 4955 5314 5473 518
Size: 906 359 159 2042
Fragments arranged by size:
2042 1116 906 709 648 359 342 278
238 159 114 86
MspA1I CmG'CkG
Cuts at: 1606 2302 3001 3186 3707 4413 5090 6238 6483
Size: 696 699 185 521 706 677 1148 245
Cuts at: 6483 1606
Size: 2120
Fragments arranged by size:
2120 1148 706 699 696 677 521 245
185
NaeI GCC'GGC
Cuts at: 2174 3313 3578 2174
Size: 1139 265 5593
Fragments arranged by size:
5593 1139 265
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6445
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6997
NgoMIV G'CCGG_C
Cuts at: 2172 3311 3576 2172
Size: 1139 265 5593
Fragments arranged by size:
5593 1139 265
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6997
NotI GC'GGCC_GC
Cuts at: 4444 4444
Size: 6997
NspI r_CATG'y
Cuts at: 1132 1229 4054 4355 5900 1132
Size: 97 2825 301 1545 2229
Fragments arranged by size:
2825 2229 1545 301 97
PasI CC'CwG_GG
Cuts at: 1348 2779 2860 3943 1348
Size: 1431 81 1083 4402
Fragments arranged by size:
4402 1431 1083 81
PciI A'CATG_T
Cuts at: 5896 5896
Size: 6997
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6997
PfoI T'CCnGG_A
Cuts at: 2265 2265
Size: 6997
PmeI GTTT'AAAC
Cuts at: 4301 4301
Size: 6997
PmlI CAC'GTG
Cuts at: 2121 2121
Size: 6997
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3378 3410 5066 5098 6579 6611 3378
Size: 32 1656 32 1481 32 3764
Fragments arranged by size:
3764 1656 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2393 3442 3711 2393
Size: 1049 269 5679
Fragments arranged by size:
5679 1049 269
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6997
PsiI TTA'TAA
Cuts at: 4567 4567
Size: 6997
PspOMI G'GGCC_C
Cuts at: 2782 2871 3171 2782
Size: 89 300 6608
Fragments arranged by size:
6608 300 89
PstI C_TGCA'G
Cuts at: 830 3006 3673 4349 830
Size: 2176 667 676 3478
Fragments arranged by size:
3478 2176 676 667
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6997
PvuII CAG'CTG
Cuts at: 2302 3001 2302
Size: 699 6298
SalI G'TCGA_C
Cuts at: 1936 4010 4339 1936
Size: 2074 329 4594
Fragments arranged by size:
4594 2074 329
SanDI GG'GwC_CC
Cuts at: 3711 3711
Size: 6997
SapI GCTCTTCn'nnn_
Cuts at: 2018 2018
Size: 6997
SbfI CC_TGCA'GG
Cuts at: 4349 4349
Size: 6997
ScaI AGT'ACT
Cuts at: 2165 3152 5245 6957 2165
Size: 987 2093 1712 2205
Fragments arranged by size:
2205 2093 1712 987
SexAI A'CCwGG_T
Cuts at: 3600 3600
Size: 6997
SfcI C'TryA_G
Cuts at: 826 1045 3002 3669 4033 4345 5480 6161 6352
Size: 219 1957 667 364 312 1135 681 191
Cuts at: 6352 826
Size: 1471
Fragments arranged by size:
1957 1471 1135 681 667 364 312 219
191
SfiI GGCCn_nnn'nGGCC
Cuts at: 4271 4271
Size: 6997
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6997
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6997
SmaI CCC'GGG
Cuts at: 1872 4324 1872
Size: 2452 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3395 3437 5101 6002 6264 820
Size: 197 926 1452 42 1664 901 262 1553
Fragments arranged by size:
1664 1553 1452 926 901 262 197 42
SnaBI TAC'GTA
Cuts at: 493 4788 493
Size: 4295 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6997
SphI G_CATG'C
Cuts at: 1132 4355 1132
Size: 3223 3774
SspI AAT'ATT
Cuts at: 5 52 4921 5
Size: 47 4869 2081
Fragments arranged by size:
4869 2081 47
StuI AGG'CCT
Cuts at: 1517 2358 2514 2946 3026 3551 1517
Size: 841 156 432 80 525 4963
Fragments arranged by size:
4963 841 525 432 156 80
StyI C'CwwG_G
Cuts at: 513 1065 4209 4436 513
Size: 552 3144 227 3074
Fragments arranged by size:
3144 3074 552 227
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2149 4029 5024 5041 5194 5379 5824
Size: 1255 569 1880 995 17 153 185 445
Cuts at: 5824 325
Size: 1498
Fragments arranged by size:
1880 1498 1255 995 569 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2163 2499 3150
Size: 274 80 33 51 1443 186 336 651
Cuts at: 3150 5243 6955 96
Size: 2093 1712 138
Fragments arranged by size:
2093 1712 1443 651 336 274 186 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2047 2079 3087 3119 6957 6989 2047
Size: 32 1008 32 3838 32 2055
Fragments arranged by size:
3838 2055 1008 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2846 1316
Size: 1530 5467
XbaI T'CTAG_A
Cuts at: 4333 4333
Size: 6997
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2915 4093 2915
Size: 1178 5819
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6997
XmaI C'CCGG_G
Cuts at: 1870 4322 1870
Size: 2452 4545
XmnI GAAnn'nnTTC
Cuts at: 5126 5126
Size: 6997
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4805 276
Size: 53 83 186 936 3271 2468
Fragments arranged by size:
3271 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI AvrII BbeI BbvCI BfrBI Bpu10I BstZ17I
DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI
NruI NsiI PacI PspXI PsrI RsrII SacI SacII
SfoI SrfI SwaI