Restriction map for
pFN21AE2085
(Circular) (Six-base) MAPSORT of: pFN21AE2085.seq Check: 1648 from: 1 to: 6982
pFN21AE2085 6982 bp
With 182 enzymes: SgfI *
August 16, 2010 14:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2668 2668
Size: 6982
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4792 278
Size: 53 83 186 936 3256 2468
Fragments arranged by size:
3256 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4325 1937
Size: 2388 4594
Acc65I G'GTAC_C
Cuts at: 4303 4303
Size: 6982
AclI AA'CG_TT
Cuts at: 1563 5109 5482 1563
Size: 3546 373 3063
Fragments arranged by size:
3546 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3638 3689 3811 5045 6429 6697 1632
Size: 2006 51 122 1234 1384 268 1917
Fragments arranged by size:
2006 1917 1384 1234 268 122 51
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6785
AflIII A'CryG_T
Cuts at: 1203 2187 2412 3913 5881 6858 1203
Size: 984 225 1501 1968 977 1327
Fragments arranged by size:
1968 1501 1327 984 977 225
AgeI A'CCGG_T
Cuts at: 5813 5813
Size: 6982
AhdI GACnn_n'nnGTC
Cuts at: 4134 5711 4134
Size: 1577 5405
AleI CACnn'nnGTG
Cuts at: 1166 2483 1166
Size: 1317 5665
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2501 3902 1184
Size: 1317 1401 4264
Fragments arranged by size:
4264 1401 1317
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3470 3502 3470
Size: 32 6950
AlwNI CAG_nnn'CTG
Cuts at: 3010 3793 3895 4725 6297 3010
Size: 783 102 830 1572 3695
Fragments arranged by size:
3695 1572 830 783 102
ApaLI G'TGCA_C
Cuts at: 3626 5039 6195 3626
Size: 1413 1156 4413
Fragments arranged by size:
4413 1413 1156
ApoI r'AATT_y
Cuts at: 1493 2705 3767 4017 4175 4291 4517 4669 6778
Size: 1212 1062 250 158 116 226 152 2109
Cuts at: 6778 1493
Size: 1697
Fragments arranged by size:
2109 1697 1212 1062 250 226 158 152
116
AseI AT'TA_AT
Cuts at: 160 1032 5536 160
Size: 872 4504 1606
Fragments arranged by size:
4504 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6982
AvaI C'yCGr_G
Cuts at: 1870 1943 2252 4307 1870
Size: 73 309 2055 4545
Fragments arranged by size:
4545 2055 309 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2954 2987 2954
Size: 33 6949
BamHI G'GATC_C
Cuts at: 4312 4679 4312
Size: 367 6615
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4303 5758 618
Size: 325 217 627 2516 1455 1842
Fragments arranged by size:
2516 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2262 2925 4301 1391
Size: 871 663 1376 4072
Fragments arranged by size:
4072 1376 871 663
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2343 2379 928
Size: 971 444 36 5531
Fragments arranged by size:
5531 971 444 36
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3677 3711 5173 5207 1903
Size: 34 1740 34 1462 34 3678
Fragments arranged by size:
3678 1740 1462 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4874 6084 4874
Size: 1210 5772
BclI T'GATC_A
Cuts at: 1548 2442 2448 1548
Size: 894 6 6082
Fragments arranged by size:
6082 894 6
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2658 5593 136
Size: 107 122 71 631 605 986 2935 1525
Fragments arranged by size:
2935 1525 986 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6977 6977
Size: 6982
BlpI GC'TnA_GC
Cuts at: 4399 4399
Size: 6982
Bme1580I G_kGCm'C
Cuts at: 1790 3630 5043 6199 1790
Size: 1840 1413 1156 2573
Fragments arranged by size:
2573 1840 1413 1156
BmgBI CAC'GTC
Cuts at: 1318 2344 1318
Size: 1026 5956
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4713 5671 442
Size: 416 53 83 399 24 3296 958 1753
Fragments arranged by size:
3296 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6982
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4146 4146
Size: 6982
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3043 3311 4212 5642 1239
Size: 1804 268 901 1430 2579
Fragments arranged by size:
2579 1804 1430 901 268
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3425 5107 5972 6270 3425
Size: 1682 865 298 4137
Fragments arranged by size:
4137 1682 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3199 5645 882
Size: 617 1700 2446 2219
Fragments arranged by size:
2446 2219 1700 617
BsaAI yAC'GTr
Cuts at: 493 1204 2190 2415 3127 3914 4276 4773 493
Size: 711 986 225 712 787 362 497 2702
Fragments arranged by size:
2702 986 787 712 711 497 362 225
BsaBI GATnn'nnATC
Cuts at: 2447 3059 3314 6976 2447
Size: 612 255 3662 2453
Fragments arranged by size:
3662 2453 612 255
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4789 5171 5806 275
Size: 53 83 186 936 3256 382 635 1451
Fragments arranged by size:
3256 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2634 5415 5813 6087 6234 6645 2634
Size: 2781 398 274 147 411 2971
Fragments arranged by size:
2971 2781 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2282 2312 2808 2838 3284
Size: 30 165 30 879 30 496 30 446
Cuts at: 3284 3314 3783 3813 1178
Size: 30 469 30 4347
Fragments arranged by size:
4347 879 496 469 446 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2270 2629 3299 3791 3851 1186
Size: 1084 359 670 492 60 4317
Fragments arranged by size:
4317 1084 670 492 359 60
BseYI C'CCAG_C
Cuts at: 6185 6681 6185
Size: 496 6486
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2186 2243 3477 1163
Size: 573 450 57 1234 4668
Fragments arranged by size:
4668 1234 573 450 57
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3057 4432 5193 6221 1540
Size: 275 186 1056 1375 761 1028 2301
Fragments arranged by size:
2301 1375 1056 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2262 3630 3801 5043 5128 6199 2262
Size: 1368 171 1242 85 1071 3045
Fragments arranged by size:
3045 1368 1242 1071 171 85
BsmI GAATG_Cn'
Cuts at: 4493 4586 4493
Size: 93 6889
BsmBI CGTCTCn'nnnn_
Cuts at: 3182 3182
Size: 6982
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2262 2925 3630 3801 4301 5043 5128
Size: 399 472 663 705 171 500 742 85
Cuts at: 5128 6199 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 705 663 500 472 399
171 85
BspEI T'CCGG_A
Cuts at: 6645 6645
Size: 6982
BspHI T'CATG_A
Cuts at: 2445 3312 4871 6601 2445
Size: 867 1559 1730 2826
Fragments arranged by size:
2826 1730 1559 867
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2668 2682 4337 844
Size: 1824 14 1655 3489
Fragments arranged by size:
3489 1824 1655 14
BsrBI CCG'CTC
Cuts at: 1394 1823 2619 3157 3295 4869 1394
Size: 429 796 538 138 1574 3507
Fragments arranged by size:
3507 1574 796 538 429 138
BsrDI GCAATG_nn'
Cuts at: 65 1248 5477 5651 65
Size: 1183 4229 174 1396
Fragments arranged by size:
4229 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5626 5813 1700
Size: 110 3816 187 2869
Fragments arranged by size:
3816 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6982
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6982
BssSI C'ACGA_G
Cuts at: 2045 2204 5042 6054 2045
Size: 159 2838 1012 2973
Fragments arranged by size:
2973 2838 1012 159
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3010 3538 794
Size: 2216 528 4238
Fragments arranged by size:
4238 2216 528
BstBI TT'CG_AA
Cuts at: 3506 5786 3506
Size: 2280 4702
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6982
BstXI CCAn_nnnn'nTGG
Cuts at: 2131 3475 2131
Size: 1344 5638
BstYI r'GATC_y
Cuts at: 1972 2355 4312 4679 5064 5081 6533 6619 6631
Size: 383 1957 367 385 17 1452 86 12
Cuts at: 6631 6977 1972
Size: 346 1977
Fragments arranged by size:
1977 1957 1452 385 383 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2021 2076 2628 3334 3618 3852
Size: 552 939 17 55 552 706 284 234
Cuts at: 3852 513
Size: 3643
Fragments arranged by size:
3643 939 706 552 552 284 234 55
17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6243
Fragments arranged by size:
6243 683 56
BtsI GCAGTG_nn'
Cuts at: 2428 4498 5292 5312 2428
Size: 2070 794 20 4098
Fragments arranged by size:
4098 2070 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6982
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3222 3257 529
Size: 35 2658 35 4254
Fragments arranged by size:
4254 2658 35 35
DraI TTT'AAA
Cuts at: 2400 3738 3751 4286 2400
Size: 1338 13 535 5096
Fragments arranged by size:
5096 1338 535 13
DraIII CAC_nnn'GTG
Cuts at: 4276 4276
Size: 6982
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5989 809
Size: 507 4673 1802
Fragments arranged by size:
4673 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2085 3602 3849
Size: 54 1425 50 275 18 255 1517 247
Cuts at: 3849 4278 4429 5318 6947 8
Size: 429 151 889 1629 43
Fragments arranged by size:
1629 1517 1425 889 429 275 255 247
151 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4429 1537
Size: 275 2617 4090
Fragments arranged by size:
4090 2617 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2709 2805 3788 4912 1353
Size: 1356 96 983 1124 3423
Fragments arranged by size:
3423 1356 1124 983 96
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 2209 5569 5943 6089 387
Size: 1064 138 435 185 3360 374 146 1280
Fragments arranged by size:
3360 1280 1064 435 374 185 146 138
EcoICRI GAG'CTC
Cuts at: 2260 2260
Size: 6982
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3043 3311 3638 3689 3811 4212 5045
Size: 393 1411 268 327 51 122 401 833
Cuts at: 5045 5642 6429 6697 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1411 833 787 597 401 393 327
268 268 122 51
EcoNI CCTnn'n_nnAGG
Cuts at: 2239 3173 2239
Size: 934 6048
EcoO109I rG'GnC_Cy
Cuts at: 2017 3560 2017
Size: 1543 5439
EcoRI G'AATT_C
Cuts at: 3767 4291 4669 3767
Size: 524 378 6080
Fragments arranged by size:
6080 524 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2050 3199 2050
Size: 1149 5833
FspI TGC'GCA
Cuts at: 3878 5488 3878
Size: 1610 5372
HaeII r_GCGC'y
Cuts at: 1678 2045 3011 6129 1678
Size: 367 966 3118 2531
Fragments arranged by size:
3118 2531 966 367
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2427 2459 3913 3945 4120
Size: 32 135 32 855 32 1454 32 175
Cuts at: 4120 4152 5623 5655 5697 5729 1373
Size: 32 1471 32 42 32 2626
Fragments arranged by size:
2626 1471 1454 855 175 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3166 4326 4572 669
Size: 988 281 1228 1160 246 3079
Fragments arranged by size:
3079 1228 1160 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6982
HpaI GTT'AAC
Cuts at: 4572 4572
Size: 6982
KpnI G_GTAC'C
Cuts at: 4307 4307
Size: 6982
MfeI C'AATT_G
Cuts at: 4581 4581
Size: 6982
MluI A'CGCG_T
Cuts at: 6858 6858
Size: 6982
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3081 3282 6096 6280 1305
Size: 1776 201 2814 184 2007
Fragments arranged by size:
2814 2007 1776 201 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3604 3851 10
Size: 54 1425 343 1772 247 3141
Fragments arranged by size:
3141 1772 1425 343 247 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2483 3617 3623 4940 5299 5458 518
Size: 648 1317 1134 6 1317 359 159 2042
Fragments arranged by size:
2042 1317 1317 1134 648 359 159 6
MspA1I CmG'CkG
Cuts at: 1606 2023 2078 2977 4171 4398 5075 6223 6468
Size: 417 55 899 1194 227 677 1148 245
Cuts at: 6468 1606
Size: 2120
Fragments arranged by size:
2120 1194 1148 899 677 417 245 227
55
NcoI C'CATG_G
Cuts at: 513 1065 2004 3618 513
Size: 552 939 1614 3877
Fragments arranged by size:
3877 1614 939 552
NdeI CA'TA_TG
Cuts at: 387 3537 387
Size: 3150 3832
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6982
NotI GC'GGCC_GC
Cuts at: 4429 4429
Size: 6982
NspI r_CATG'y
Cuts at: 1132 1229 2665 3242 3487 3731 4340 5885 1132
Size: 97 1436 577 245 244 609 1545 2229
Fragments arranged by size:
2229 1545 1436 609 577 245 244 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6982
PciI A'CATG_T
Cuts at: 5881 5881
Size: 6982
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2272 2935 3793 4276 1108
Size: 1164 663 858 483 3814
Fragments arranged by size:
3814 1164 858 663 483
PmeI GTTT'AAAC
Cuts at: 4286 4286
Size: 6982
PmlI CAC'GTG
Cuts at: 2190 2415 3127 3914 2190
Size: 225 712 787 5258
Fragments arranged by size:
5258 787 712 225
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5051 5083 6564 6596 5051
Size: 32 1481 32 5437
Fragments arranged by size:
5437 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6982
PsiI TTA'TAA
Cuts at: 3363 4552 3363
Size: 1189 5793
PspXI vC'TCGA_Gb
Cuts at: 2252 2252
Size: 6982
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2963 2995 2963
Size: 32 6950
PstI C_TGCA'G
Cuts at: 830 2435 2493 2679 2916 3018 3223 3891 4334
Size: 1605 58 186 237 102 205 668 443
Cuts at: 4334 830
Size: 3478
Fragments arranged by size:
3478 1605 668 443 237 205 186 102
58
PvuI CG_AT'CG
Cuts at: 2001 3057 2001
Size: 1056 5926
PvuII CAG'CTG
Cuts at: 2977 4171 2977
Size: 1194 5788
SacI G_AGCT'C
Cuts at: 2262 2262
Size: 6982
SacII CC_GC'GG
Cuts at: 2024 2079 2024
Size: 55 6927
SalI G'TCGA_C
Cuts at: 1936 4324 1936
Size: 2388 4594
SbfI CC_TGCA'GG
Cuts at: 2679 4334 2679
Size: 1655 5327
ScaI AGT'ACT
Cuts at: 5230 6942 5230
Size: 1712 5270
SfcI C'TryA_G
Cuts at: 826 1045 2431 2489 2675 2912 3014 3219 3564
Size: 219 1386 58 186 237 102 205 345
Cuts at: 3564 3887 4330 5465 6146 6337 826
Size: 323 443 1135 681 191 1471
Fragments arranged by size:
1471 1386 1135 681 443 345 323 237
219 205 191 186 102 58
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6982
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6982
SmaI CCC'GGG
Cuts at: 1872 4309 1872
Size: 2437 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2252 3440 5086 5987 6249 820
Size: 197 926 309 1188 1646 901 262 1553
Fragments arranged by size:
1646 1553 1188 926 901 309 262 197
SnaBI TAC'GTA
Cuts at: 493 4773 493
Size: 4280 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6982
SphI G_CATG'C
Cuts at: 1132 2665 4340 1132
Size: 1533 1675 3774
Fragments arranged by size:
3774 1675 1533
SspI AAT'ATT
Cuts at: 5 52 4906 5
Size: 47 4854 2081
Fragments arranged by size:
4854 2081 47
StuI AGG'CCT
Cuts at: 1517 2330 1517
Size: 813 6169
StyI C'CwwG_G
Cuts at: 513 1065 2004 3618 4421 513
Size: 552 939 1614 803 3074
Fragments arranged by size:
3074 1614 939 803 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5009 5026 5179 5364 5809 325
Size: 1255 3429 17 153 185 445 1498
Fragments arranged by size:
3429 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3030 5228 6940
Size: 274 80 33 51 1443 1053 2198 1712
Cuts at: 6940 96
Size: 138
Fragments arranged by size:
2198 1712 1443 1053 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2282 2314 3783 3815 3930 3962 6942 6974 2282
Size: 32 1469 32 115 32 2980 32 2290
Fragments arranged by size:
2980 2290 1469 115 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2342 3086 1316
Size: 1026 744 5212
Fragments arranged by size:
5212 1026 744
XbaI T'CTAG_A
Cuts at: 4318 4318
Size: 6982
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2518 2518
Size: 6982
XhoI C'TCGA_G
Cuts at: 1943 2252 1943
Size: 309 6673
XmaI C'CCGG_G
Cuts at: 1870 4307 1870
Size: 2437 4545
XmnI GAAnn'nnTTC
Cuts at: 3767 5111 3767
Size: 1344 5638
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4790 276
Size: 53 83 186 936 3256 2468
Fragments arranged by size:
3256 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI
PvuI PvuII SacI SacII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BbeI BbvCI BfrBI Bpu10I
BsiWI BstZ17I Bsu36I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI
RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI