Restriction map for
pFN21AE2084
(Circular) (Six-base) MAPSORT of: pFN21AE2084.seq Check: 1510 from: 1 to: 6982
pFN21AE2084 6982 bp
With 182 enzymes: SgfI *
December 8, 2014 13:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3680 3680
Size: 6982
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4792 278
Size: 53 83 186 936 3256 2468
Fragments arranged by size:
3256 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3123 3452 4325 1937
Size: 1186 329 873 4594
Fragments arranged by size:
4594 1186 873 329
Acc65I G'GTAC_C
Cuts at: 4303 4303
Size: 6982
AclI AA'CG_TT
Cuts at: 1563 5109 5482 1563
Size: 3546 373 3063
Fragments arranged by size:
3546 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2126 5045 6429 6697 1632
Size: 494 2919 1384 268 1917
Fragments arranged by size:
2919 1917 1384 494 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6785
AflIII A'CryG_T
Cuts at: 1203 5881 6858 1203
Size: 4678 977 1327
Fragments arranged by size:
4678 1327 977
AgeI A'CCGG_T
Cuts at: 5813 5813
Size: 6982
AhdI GACnn_n'nnGTC
Cuts at: 5711 5711
Size: 6982
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6982
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6982
AlwNI CAG_nnn'CTG
Cuts at: 2893 4725 6297 2893
Size: 1832 1572 3578
Fragments arranged by size:
3578 1832 1572
ApaLI G'TGCA_C
Cuts at: 5039 6195 5039
Size: 1156 5826
ApoI r'AATT_y
Cuts at: 1493 2346 2421 2660 2734 2818 3059 3289 3518
Size: 853 75 239 74 84 241 230 229
Cuts at: 3518 4066 4211 4291 4517 4669 6778 1493
Size: 548 145 80 226 152 2109 1697
Fragments arranged by size:
2109 1697 853 548 241 239 230 229
226 152 145 84 80 75 74
AseI AT'TA_AT
Cuts at: 160 1032 5536 160
Size: 872 4504 1606
Fragments arranged by size:
4504 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6982
AvaI C'yCGr_G
Cuts at: 1870 1943 2023 2038 3891 4307 1870
Size: 73 80 15 1853 416 4545
Fragments arranged by size:
4545 1853 416 80 73 15
BamHI G'GATC_C
Cuts at: 2596 4312 4679 2596
Size: 1716 367 4899
Fragments arranged by size:
4899 1716 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2042 3668 4303 5758 618
Size: 325 217 627 255 1626 635 1455 1842
Fragments arranged by size:
1842 1626 1455 635 627 325 255 217
BanII G_rGCy'C
Cuts at: 1391 4301 1391
Size: 2910 4072
BbeI G_GCGC'C
Cuts at: 2046 2046
Size: 6982
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3252 3876 928
Size: 971 1353 624 4034
Fragments arranged by size:
4034 1353 971 624
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5173 5207 1903
Size: 34 3236 34 3678
Fragments arranged by size:
3678 3236 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4874 6084 4874
Size: 1210 5772
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6982
BfrBI ATG'CAT
Cuts at: 3374 3374
Size: 6982
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 5593 136
Size: 107 122 71 631 605 337 3584 1525
Fragments arranged by size:
3584 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 4201 6977 4201
Size: 2776 4206
BlpI GC'TnA_GC
Cuts at: 3908 4399 3908
Size: 491 6491
Bme1580I G_kGCm'C
Cuts at: 1790 5043 6199 1790
Size: 3253 1156 2573
Fragments arranged by size:
3253 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6982
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2866 4713 5671
Size: 416 53 83 399 24 1449 1847 958
Cuts at: 5671 442
Size: 1753
Fragments arranged by size:
1847 1753 1449 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6982
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2884 2884
Size: 6982
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5642 1239
Size: 4403 2579
Bpu10I CC'TnA_GC
Cuts at: 2709 3703 2709
Size: 994 5988
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5107 5972 6270 5107
Size: 865 298 5819
Fragments arranged by size:
5819 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5645 882
Size: 617 4146 2219
Fragments arranged by size:
4146 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4773 493
Size: 711 3569 2702
Fragments arranged by size:
3569 2702 711
BsaBI GATnn'nnATC
Cuts at: 2550 6976 2550
Size: 4426 2556
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2043 4789 5171 5806
Size: 53 83 186 936 510 2746 382 635
Cuts at: 5806 275
Size: 1451
Fragments arranged by size:
2746 1451 936 635 510 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5415 5813 6087 6234 6645 5415
Size: 398 274 147 411 5752
Fragments arranged by size:
5752 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3922 3952 1178
Size: 30 165 30 2519 30 4208
Fragments arranged by size:
4208 2519 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2038 2041 2044 2144 2606 1186
Size: 852 3 3 100 462 5562
Fragments arranged by size:
5562 852 462 100 3 3
BseYI C'CCAG_C
Cuts at: 6185 6681 6185
Size: 496 6486
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6409
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4432 5193 6221 1540
Size: 275 186 2431 761 1028 2301
Fragments arranged by size:
2431 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2030 5043 5128 6199 2030
Size: 3013 85 1071 2813
Fragments arranged by size:
3013 2813 1071 85
BsmI GAATG_Cn'
Cuts at: 2489 2527 3374 4493 4586 2489
Size: 38 847 1119 93 4885
Fragments arranged by size:
4885 1119 847 93 38
BsmBI CGTCTCn'nnnn_
Cuts at: 3443 3443
Size: 6982
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2030 4301 5043 5128 6199 1391
Size: 399 240 2271 742 85 1071 2174
Fragments arranged by size:
2271 2174 1071 742 399 240 85
BspEI T'CCGG_A
Cuts at: 6645 6645
Size: 6982
BspHI T'CATG_A
Cuts at: 3285 3343 3865 4871 6601 3285
Size: 58 522 1006 1730 3666
Fragments arranged by size:
3666 1730 1006 522 58
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3680 4337 844
Size: 2836 657 3489
Fragments arranged by size:
3489 2836 657
BsrBI CCG'CTC
Cuts at: 1394 1823 4869 1394
Size: 429 3046 3507
Fragments arranged by size:
3507 3046 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2699 3649 3837 5477 5651 65
Size: 1183 1451 950 188 1640 174 1396
Fragments arranged by size:
1640 1451 1396 1183 950 188 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5626 5813 1700
Size: 110 3816 187 2869
Fragments arranged by size:
3816 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6982
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6982
BssSI C'ACGA_G
Cuts at: 2739 5042 6054 2739
Size: 2303 1012 3667
Fragments arranged by size:
3667 2303 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3311 794
Size: 2517 4465
BstBI TT'CG_AA
Cuts at: 5786 5786
Size: 6982
BstEII G'GTnAC_C
Cuts at: 1184 2924 1184
Size: 1740 5242
BstXI CCAn_nnnn'nTGG
Cuts at: 2474 3823 2474
Size: 1349 5633
BstYI r'GATC_y
Cuts at: 1972 2596 4201 4312 4679 5064 5081 6533 6619
Size: 624 1605 111 367 385 17 1452 86
Cuts at: 6619 6631 6977 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1605 1452 624 385 367 346 111
86 17 12
BstZ17I GTA'TAC
Cuts at: 3453 3453
Size: 6982
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6430
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6243
Fragments arranged by size:
6243 683 56
BtsI GCAGTG_nn'
Cuts at: 4498 5292 5312 4498
Size: 794 20 6168
Fragments arranged by size:
6168 794 20
ClaI AT'CG_AT
Cuts at: 1554 2554 1554
Size: 1000 5982
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6947
DraI TTT'AAA
Cuts at: 2634 4053 4286 2634
Size: 1419 233 5330
Fragments arranged by size:
5330 1419 233
DraIII CAC_nnn'GTG
Cuts at: 2493 2493
Size: 6982
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5989 809
Size: 507 4673 1802
Fragments arranged by size:
4673 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2562 2984 4429
Size: 54 1425 50 275 18 732 422 1445
Cuts at: 4429 5318 6947 8
Size: 889 1629 43
Fragments arranged by size:
1629 1445 1425 889 732 422 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4429 1537
Size: 275 2617 4090
Fragments arranged by size:
4090 2617 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2083 4073 4912 1353
Size: 730 1990 839 3423
Fragments arranged by size:
3423 1990 839 730
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5569 5943 6089 387
Size: 1064 138 3980 374 146 1280
Fragments arranged by size:
3980 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2126 5045 5642 6429 6697 1239
Size: 393 494 2919 597 787 268 1524
Fragments arranged by size:
2919 1524 787 597 494 393 268
EcoRI G'AATT_C
Cuts at: 2346 2660 2734 3289 4291 4669 2346
Size: 314 74 555 1002 378 4659
Fragments arranged by size:
4659 1002 555 378 314 74
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2173 2173
Size: 6982
FspI TGC'GCA
Cuts at: 5488 5488
Size: 6982
HaeII r_GCGC'y
Cuts at: 1678 2046 2105 6129 1678
Size: 368 59 4024 2531
Fragments arranged by size:
4024 2531 368 59
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3847 3879 3997 4029 5623
Size: 32 135 32 2275 32 118 32 1594
Cuts at: 5623 5655 5697 5729 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2275 1594 135 118 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2586 4326 4572 669
Size: 988 281 648 1740 246 3079
Fragments arranged by size:
3079 1740 988 648 281 246
HindIII A'AGCT_T
Cuts at: 748 2150 3844 748
Size: 1402 1694 3886
Fragments arranged by size:
3886 1694 1402
HpaI GTT'AAC
Cuts at: 2586 4572 2586
Size: 1986 4996
KasI G'GCGC_C
Cuts at: 2042 2042
Size: 6982
KpnI G_GTAC'C
Cuts at: 4307 4307
Size: 6982
MfeI C'AATT_G
Cuts at: 3558 4581 3558
Size: 1023 5959
MluI A'CGCG_T
Cuts at: 6858 6858
Size: 6982
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3222 6096 6280 1305
Size: 1917 2874 184 2007
Fragments arranged by size:
2874 2007 1917 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2564 2986 10
Size: 54 1425 343 732 422 4006
Fragments arranged by size:
4006 1425 732 422 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4940 5299 5458 518
Size: 648 3774 359 159 2042
Fragments arranged by size:
3774 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4398 5075 6223 6468 1606
Size: 2792 677 1148 245 2120
Fragments arranged by size:
2792 2120 1148 677 245
NarI GG'CG_CC
Cuts at: 2043 2043
Size: 6982
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6430
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6982
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6982
NotI GC'GGCC_GC
Cuts at: 4429 4429
Size: 6982
NsiI A_TGCA'T
Cuts at: 3376 3376
Size: 6982
NspI r_CATG'y
Cuts at: 1132 1229 3713 4340 5885 1132
Size: 97 2484 627 1545 2229
Fragments arranged by size:
2484 2229 1545 627 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6982
PciI A'CATG_T
Cuts at: 5881 5881
Size: 6982
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6982
PmeI GTTT'AAAC
Cuts at: 4286 4286
Size: 6982
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5051 5083 6564 6596 5051
Size: 32 1481 32 5437
Fragments arranged by size:
5437 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2917 1613
Size: 1304 5678
PsiI TTA'TAA
Cuts at: 4552 4552
Size: 6982
PspXI vC'TCGA_Gb
Cuts at: 3891 3891
Size: 6982
PstI C_TGCA'G
Cuts at: 830 2307 3756 4334 830
Size: 1477 1449 578 3478
Fragments arranged by size:
3478 1477 1449 578
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6982
SalI G'TCGA_C
Cuts at: 1936 4324 1936
Size: 2388 4594
SbfI CC_TGCA'GG
Cuts at: 4334 4334
Size: 6982
ScaI AGT'ACT
Cuts at: 5230 6942 5230
Size: 1712 5270
SfcI C'TryA_G
Cuts at: 826 1045 2303 3752 4330 5465 6146 6337 826
Size: 219 1258 1449 578 1135 681 191 1471
Fragments arranged by size:
1471 1449 1258 1135 681 578 219 191
SfoI GGC'GCC
Cuts at: 2044 2044
Size: 6982
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6982
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6982
SmaI CCC'GGG
Cuts at: 1872 4309 1872
Size: 2437 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3891 5086 5987 6249 820
Size: 197 926 1948 1195 901 262 1553
Fragments arranged by size:
1948 1553 1195 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4773 493
Size: 4280 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6982
SphI G_CATG'C
Cuts at: 1132 4340 1132
Size: 3208 3774
SspI AAT'ATT
Cuts at: 5 52 4906 5
Size: 47 4854 2081
Fragments arranged by size:
4854 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6982
StyI C'CwwG_G
Cuts at: 513 1065 4421 513
Size: 552 3356 3074
Fragments arranged by size:
3356 3074 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5009 5026 5179 5364 5809 325
Size: 1255 3429 17 153 185 445 1498
Fragments arranged by size:
3429 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4157 5228 6940
Size: 274 80 33 51 1443 2180 1071 1712
Cuts at: 6940 96
Size: 138
Fragments arranged by size:
2180 1712 1443 1071 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2725 2757 6942 6974 2725
Size: 32 4185 32 2733
Fragments arranged by size:
4185 2733 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6982
XbaI T'CTAG_A
Cuts at: 4318 4318
Size: 6982
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3466 3662 3823 3466
Size: 196 161 6625
Fragments arranged by size:
6625 196 161
XhoI C'TCGA_G
Cuts at: 1943 3891 1943
Size: 1948 5034
XmaI C'CCGG_G
Cuts at: 1870 4307 1870
Size: 2437 4545
XmnI GAAnn'nnTTC
Cuts at: 2153 2346 2754 5111 2153
Size: 193 408 2357 4024
Fragments arranged by size:
4024 2357 408 193
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4790 276
Size: 53 83 186 936 3256 2468
Fragments arranged by size:
3256 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI
PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BsiWI
Bsu36I EcoICRI EcoNI EcoO109I EcoRV FseI FspAI NaeI
NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SrfI SwaI